Mbd5 (NM_029924) Mouse Untagged Clone

CAT#: MC224878

Mbd5 (untagged) - Mouse methyl-CpG binding domain protein 5 (Mbd5), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mbd5
• Synonyms: 9430004D19Rik; AA536666; AI426407; C030040A15Rik; Gm1630; OTTMUSG00000012483
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224878 representing NM_029924
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGGAGGCAAAGAGTGTGACGGAGGAGACAAGGAAGGAGGTCTTGCAGCTATCCAGGTTCCTGTGG
  GTTGGCAGCGGCGTGTGGACCATAATGGCGTGCTGTACATCAGTCCCAGTGGGTCTTTGTTATCTTGCTT
  GGACCAGGTTAAGACATACCTGCTCACTGACGGAACATGCAAGTGTGGCTTGGAATGTCCTCTTATTCTT
  CCCAAGGTGTTTAATTTTGATCCTGGAGCTGCTGTGAAGCAGAGAACTGCAGAAGATGTTAAGGCAGATG
  ATGATGTCACAAAGCTATGCATACATAAAAGAAAGATCATTGCAGTGGCCACACTCCATCAAAGCATGGA
  GGCCCCGCATCCTTCTCTGGTGCTCACCAGTCCTGGAGGAGGAACAAATGCAACTCCAGTAGTACCTTCA
  CGGGCGGCAACTCCAAGATCAGTAAGAAACAAGTCTCATGAAGGAATTACAAATTCTGTAATGCCAGAAT
  GTAAGAATCCTTTCAAGTTAATGACTGGATCATCAAATGCCATGGGAAGGCTATACATGCAAGACCTGCC
  TGGAAGCCAGCAGCAGGAGCTCCACCCTGTCTACCCCCGCCAGAGATTGGGCAGTAGTGAGCATGGACAG
  AAATCTCCATTTCGTGGCAGCCATGGAGGCCTGCCTAGCCCAGCATCATCAGGGTCTCAGATCTATGGAG
  ATGGCTCAATCTCTCCAAGGACTGACCCGCTCGGAAGCCCTGATGTCTTCACAAGAAATAACCCTGGTTT
  TCATGGAGCTCCCAATTCCAGCCCTATTCACCTAAATAGGACTCCTCTTTCTCCACCTTCAGTAATGCTA
  CATGGTTCTCCCGTACAGTCATCCTGTGCAATGGCTGGAAGAACTAATATACCTCTTTCCCCAACCTTGA
  CTACAAAGAGTCCAGTAATGAAAAAACCAATGTGTAATTTTTCAACTAATATGGAAATACCACGAGCAAT
  GTTCCACCACAAACCACCCCAAGGCCCACCTCCCCCTCCTCCACCTTCTTGTGCTCTTCAGAAAAAGCCA
  TTAACATCTGAGAAAGATCCACTTGGCATTCTTGACCCTATTCCTAGTAAACCAGTGAATCAGAACCCTA
  TTATCATTAATCCAACCAGTTTCCATTCAAATGTCCACTCTCAGGTACCTGTGATGAATGTAAGCATGCC
  TCCTGCTGTTGTTCCTTTGCCAAGTAATCTCCCTTTGCCAACTGTAAAACCTGGTCATATGAATCATGGG
  AGTCATGTACAAAGAATTCAGCATTCAGCTTCAACCTCCCTGTCCCCTTCTCCAGTGACATCCCCAGTGC
  ACATGATGGGGACTGGAATTGGAAGGATTGAGGCATCGCCCCAAAGATCACGCTCATCTTCCACATCATC
  AGATCATGGAAATTTCATGATGCCACCTGTAGGACCCCAGGCCACTTGTAGTGGCATAAAGGTTCCACCT
  AGGTCACCAAGGTCAACAATAGGTTCCCCAAGGCCATCAATGCCATCAAGCCCTTCTACCAAGTCTGATG
  GACATCATCAGTACAAGGATATCCCTAACCCATTAATTGCTGGAATGAGTAATGTACTCAATACCCCAAG
  CAGTGCAGCTTTTCCTACTGCACCTGCCGGAAATGGTTCTGTAAAGAGTCAGCCTGGTTTGCTGGGAATG
  CCTTTAAATCAGATCTTGAACCAGCACAATGCTGCCTCCTTTCCAGCAAGTAGTTTACTCTCAGCAGCAG
  CCAAAGCACAGCTAGCAAATCAAAACAAACTTGCTGGTAACAACAGTAGCAGCAGTAACAATTCTGGAGC
  TGTTGCCAGCAGCGGCAACACTGAAGGACACAGCACTTTAAACACCATGTTCCCTCCAACTGCCAACATG
  CTTCTCCCAACAGGTGAAGGTCAAAGTGGTCGAGCAGCACTAAGAGATAAGTTGATGTCTCAGCAAAAAG
  ACTCATTACGGAAAAGAAAGCAGCCACCCACCACAGTGTTGAGTTTGCTCAGACAGTCCCAAATGGATAG
  TTCTGCAGCTCCTAAACCTGGACCTGACTTGCTAAGGAAGCATGGGCAGGGGTCATTTCCCATCAGTTCA
  ATGTCTCAGTTACTACAGTCTATGAGTTGTCAAAGCTCTCACTTGAGTAGCAATAGTACCCCGGGTTGTG
  GGGGATCAAATACTGCTTTGCCTTGCTCTGCTAACCAGCTGCATTTTCCAGATCCTAATATGAATTCCAC
  TGTTCTTCAGAATTCACTGACACAGAGCATACCTTTAAGAGGGGAAGCCGTGCACTGCCACAATGCAAAC
  ACTAACTTTGTTCACAGTAACAGTCCAGTCCCCAACCACCATCTTGCAGGTTTAATAAATCAGATTCAGG
  CTAGCGGGAACTGTGGGATGCTCAGTCAGTCGGGCATGGCTTTAGGAAACTCATTACATCCCAATCCACC
  TCAGTCAAGAATTTCAACGTCCTCCACTCCAGTGATACCAAACAGCATTGTTAGCAGCTATAATCAAACA
  AGTTCTGAAGCAGGTGGTTCAAGCCTGCCGTCCTCCATAGCTATAGCTGGCAGCAACCACCCTGCCATCA
  CAAAGACAACATCTGTTCTCCAAGATGGTGTCATAGTCACCACCGCAGCTGGAAACCCACTGCAGAGTCA
  GCTGCCCATTGGGAGTGACTTTCCTTTTGTTGGCCAAGAGCATGCCCTTCATTTTCCATCCAACAGCACC
  GCAAACAACCATCTTCCACACCCCTTGAACCCCAGCCTCCTCAGCTCTCTACCTATCTCTTTGCCAGTGA
  ATCAACAGCATCTCCTAAACCAGAATCTATTAAATATCCTCCAGCCTTCAGCAGGAGAAGGCAAGTCTGA
  GATCAACCTCCACCCTTTAGGTTTTCTCAACCCGAATGTAAACGCTGCTTTAGCTTTCCTCTCCGGTGAC
  GTGGATGGGCAGGTATTACAGCCTGTCCACTTTCAGCTCTTAGCAGCCCTGCTTCAGAACCAAGCCCAAG
  CAGCTGCCATGCTTCCCCTGCCGTCTTTCAATCTGACCATCTCAGATCTTTTGCAACCGCAAAATACCCC
  TTTACCCTTATTAACACAGATGACAGCCCCACCAGACCGTTTGCCAAGCAATCAGTCAGACAACAGCCGA
  GCTGATACCCTTTTAGGCAGCCCCCTGGGAAACCCTTTAGCAGGCAGTGACACTACTTTTAACCCCCTGC
  TCCTCCCAGCTGTCACTGGGGCCTCAGGATTAATGGCCTTGAATCCCCAGCTGTTGGGGGGTGTCCTGAA
  CTCGGCATCGGCCAACACCGCTATTCATCCAGAGGTTTCCATAGCAACCTCCTCCCAGGCAACCACTACC
  ACAACCACTACATCATCAGCAGTGGCAGCACTGACTGTCTCAACACTTGGTGGGACAGCAGTGGTGTCAA
  TGGCAGAAACATTGCTGAATATATCTAATAATGCTGGGAATCCACCTGGTCCAGCTAAACTCAACAGTAA
  CTCTATGGTGCCACAGCTACTTAACCCTCTACTGGGGACAGGTCTGCTTGGTGATATATCATCCATAAAC
  AATTCATTGAATAACCATCAACTGACTCATCTACAGTCGCTACTAAACAGCAATCAGATGTTTCCTCCAA
  ATCAGCAGCCGCAGCAACACCTTCTCCAGGGACACCAGAATCTCCAGGCCTTCCAAGGACAACCCACAGT
  CCCTTGCCCAGCTAACAATAACCCCATGGCTTGTCTGTTTCAGAACTTCCAGGTGAGGATGCAAGGAGAT
  GCAGCTCTTCTAAATAAAAGAATAAGCACTCAGCCTGGGCTCACAACACTTCCTGAAAACCCAAACCTTG
  CACTTCCACATTTCCAAGATACACCCTGTGAGCTGCAGCCAAGGATTGACCTTGGTCAGCCTATGAAGGA
  CGGCCTCGTTATGGGTGGCCAAGGAGATGCCTCCGTAGACGCCATTTACAAAGCAGTTGTGGATGCAGCC
  AGCAAAGGAATGCAGGTTGTCATCACCACTGCTGTCAACAGCACGACTCAAATCAGCCCTATCCCAGCCC
  TGAGCGCCATGAGTGCCTTCACTGCCTCCATTGGTGACCCATTAAATCTGTCCAGTGCTGTGAGCGCTGT
  CATCCACGGGCGGAACATGGGAGGAGTGGATCATGATGGCAGGCTGAGGAATGCGAGAGGGGCACGGCTA
  CCCAAGAACATAGACCATGGGAAGAACTCCAGCGAAGGAGACGGGTTTGAGTGTTTCAAGTCAGCGAGCT
  GCCACACATCCAGAAAACAGTGGGATGGGGAGCAAAGTCCCAGAGGGGAGCGCAACAGGTGGAAGTATGA
  GGAGTTTTTAGATCATCCAGGCCATATCCACAGTAGTCCTTGCCACGAAAGGCCCAACAATGTCTCTACA
  CTGCCATTTCTAGCTGGGGAACAGCACCCGATACTGTTACCACCAAGAAACTGTCAAGGTGATAAGATTC
  TGGAGGAAAATTTCAGGTATAATAATTACAAAAGAACTATGATGAGTTTTAAAGAGAGACTAGAGAGTAC
  TGTGGAGAGATGTACACACATTAACGGAAACAGACCTCGTCAGAGTCGGGGCTTTGGAGAGCTGCTAGGC
  ACTGCAAAACAAGACCTAGTTCTAGAGGGGCAGTCTCCGGGTTCTTCAAATAGTTTGGAAAGTTCTCTGG
  TCAAAGACTACATCCATTACAATGGAGACTTTAATGCCAAAACCATTAATGGGTGTGTGCCTAGTCCCTC
  AGATGCTAAAAGCATTAGTAGTGAAGACGACCTACGGAATCCAGACTCCCCCTCCTCACATGAGTTGATA
  CACTATAGACCAAGGACGTTCAACGTTGGCGACTTGGTCTGGGGCCAAATCAAAGGACTGACTTCCTGGC
  CTGGGAAATTCATAAGAGAAGACGACGTTCACAATTCATGCCAACAAAGCCCCGAGGAAGGGAAGGTGGA
  GCCCGAGAAGTTGAAGACTCTCACAGAAGGTTTAGAAGCCTACAGCCGCGTCCGGAAAAGGAGCAGAAAA
  AGTGGGAAACTAAATAACCATTTAGAAGCTGCTATTCACGAGGCCATGAGCGAACTGGACAAAATGTCTG
  GGACTGTTCACCAAATCCCACAGGGTGACAGACAAATGAGGCCCCCCAAACCCAAGAGGAGGAAGATCTC
  CAGATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_029924
• Insert Size: 5187 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029924.2, NP_084200.1
• RefSeq Size: 7055 bp
• RefSeq ORF: 5187 bp
• Locus ID: 109241
• Cytogenetics: 2 C1.1
• Gene Summary: Binds to heterochromatin. Does not interact with either methylated or unmethylated DNA (in vitro) (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). This variant has an inferred 5' UTR exon combination based on the mRNA AK155574.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.