Tiam2 (NM_011878) Mouse Untagged Clone

CAT#: MC224853

Tiam2 (untagged) - Mouse T-cell lymphoma invasion and metastasis 2 (Tiam2), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tiam2
• Synonyms: 3000002F19Rik; mKIAA2016; STEF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224853 representing NM_011878
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGAACTCCGAGAGTCAATATACCTTCCAAGGATCCAAGAATCATAGTAATACTGTCACTGGTGCTA
  AGCAAAAGCCTTGCTCTCTGAAAATACGCAGCGTTCATGCAAAAGACGAGAAGTCCTTACATGGTTGGAC
  TCATGGGAGCAGCGGTGCAGGCTACAAGTCCAGGTCCCTAGCCCGAAGCTGCCTTTCTCACTTTAAGAAT
  CACCAGCCTTACGCCACCAGACTCAGTGGACCCACATGTAAAGTCTCAAAGGGCACCACCTACTCTAAGC
  ACAGAGCAAATACCCCGGGAAATGATTTCCAGGGCAACAGTGGTGCTTTCTTACCTGAGAATGGCTTCCA
  CTATGTTGACCGCGAGTCAGAGGAAAGCCATATCACCTCCAATGGGCACCTTCTCACCTGCTATGGGAGA
  AAGGAAAGCCTCGCCTCCACTCCTCCAGGCGAGGACCACAGGAGCCCCAGGGTGCTCATCAAGACCCTGG
  GAAAGCTAGACGGGTGTTTAAGAGTCGAGTTCCACAACGGTGGCAACCCCCACAAGGGGACCTCCGAGGA
  CCCCAGTGGACCTGTACGGCTGCTGAGATACTCCCCTACCTTAGCATCGGAAACCTGCCCGGTGCGGGAA
  ACCAGGCGGCATTCCGCTGCAGGCTCCCCATCCAGCCAGCGGCCCTCTCCCACTGACTCTCGCCTGCGCT
  CCAGCAAAGGCAGCTCCCTGAGCTCAGAGTCATCCTGGTATGACTCCCCCTGGGGCAACGCTGGGGAGGT
  GAGCGAGGTGGAGGGCTCCTTCCTGGCTCCCAGCACTCCAGACCCCAGCCTCCCCAGCAGCTTCCCACCC
  AGTGACACCAAAAAGCCTTTCAACCAAAGCTCTTCCCTCTCCTCCCTCCGGGAATTGTACAAAGATCCCA
  ACCTGGGGTGCCGCTCACCTTCCGGCACCTGCCTTTCTTCCAATGAGTACATCAGCTCTCAAGTCAGCCT
  GAACAACCGAGTCTCCTTTGCGTCTGACATGGATGTGCCCTCCAGGGTGGATCACAGGGATCCCCTGCAC
  TATAGTTCCTTTACTCTCCCCTGTCGCAAGTCCAAAGCCTTAACTGAAGATGCAGCTAAGAAAGACACCC
  TCAAAGCCAGAATGCGGCGCTTCAGTGACTGGACAGGAAGCCTCTCCAGGAAGAAGAGGAAACTGCAGGA
  ACCCAGGTCCATGGAGGGCAGTGAGTACTTTGATAGCCACTCAGATGGACTGAATGCAGAAGGGCAGGTG
  CCCGCGCAGACATCTTCCTTACTGTGGTCAGGGGGCTCGGCTCAGACCCTGCCTCACAGAAGCGAATCCA
  CTCACGCTATCAGCGTCGATCCCCTCCGACAGAACATCTATGAGAATTTCATGCGAGAGCTCGAAATGAG
  CAGGAGCAACACAGAACACGTGGAAACTTCCACAGAGACCATGGAGTCCAGCAGCGAGTCTGTCAGCTCA
  CTGGAGCAGCTGGATCTGCTCTTTGAGAAGGAGCAGGGAGTGGTCCGGAAAGCCGGGTGGCTCTTCTTCA
  AACCCCTTGTCACCTTGCAGAAGGAGAGGAAACTGGAGCTGGTGGCTCGGAGGAAGTGGAAACAATACTG
  GGTGACCCTGAAAGGCTGTACTCTGCTGTTTTATGAGACCTATGGAAAGAATTCCACAGAGCAGAATAGT
  GCCCCACGGTGTGCCCTCTTTGCAGAGGACAGCATCGTGCAGTCTGTCCCAGAGCATCCCAAGAAGGAAC
  ACGTGTTCTGCCTGAGTAACTCCTGTGGGGACGTCTACCTATTCCAGGCCACTAGCCAGACAGATCTGGA
  AAACTGGGTCACAGCCATCCACTCGGCGTGCGCATCCCTCTTTGCAAAGAAGCACGGAAAGGAGGACACG
  GTGCGACTGCTAAAGAGCCAGACCAGAAGCCTGCTTCAGAAGATAGACATGGATAGCAAGATGAAGAAGA
  TGGCAGAGTTGCAGCTGTCTGTGGTGAGCGACCCCAAGAACAGGAAGGCCATCGAGAATCAGATCCGGCA
  ATGGGAGCAGAATCTGGAAAAATTCCACATGGACCTGTTCCGCATGCGCTGCTATTTGGCGAGCTTACAA
  GGTGGGGAGTTACCAAATCCCAAGAGTCTCCTTGCTGCCACCAGCCGCCCCTCCAAGCTGGCTCTTGGCA
  GGCTGGGCGTCCTGTCTGTTTCGTCTTTCCATGCTCTGGTGTGTTCCAGAGATGATTCCACTCTCAGGAA
  AAGAACACTTTCCCTTACCCAGAGAGGAAAAAGCAAGAAAGGCATATTTTCTTCATTGAAAGGTCTGGAC
  ACCCTAGCAAGAAAGGGTAGGGAGAAGAGAGCTTCCATAACTCAGATGTTTGATTCTAGCCACAGCCATG
  GATTTCTTGGAACTCAGCTACCTCAAAAGTCCACTAACTCCAACAAGGCCCATGACCTGCATCTGTATGG
  CTCCGCAGTAGACAGCGCGCTGCGAGACAGCATGTGGGAAGTCCAGACTTATGTCCACTTCCAGGATAAC
  GAAGGAGTTACTGTGACCATCAAGCCAGAGCACAGGGTGGAAGATGTTCTGGCTTTGGTGTGCAAGATGA
  GACAGTTGGAACCCACTCACTATGGTCTTCAGCTCCGAAAGGTGGTCGATAAAAGTGTGGAGTGGTGTGT
  GCCCGCGCTGTATGAATACATGCAAGAGCAGGTTTATGATGAAATCGAAGTTTTCCCACTCAGTGTGTAT
  GACGTGCAGCTAACCAAGACTGGGGACATGACTGACTTTGGGTTTGCAGTCACAGTTCAGGTGGACGAAC
  ACCAGCATCTCAACCGGATATTTATCAGTGATGTTCTCCCTGACAGCCTGGCATACGGAGGAGGGCTGAG
  AAAGGGCAACGAAATCACAAGCTTAAATGGGGAACCAGTGTCTGACCTTGACATCCAGCAGATGGAGGCT
  TTGTTTTCTGAGAAGAGCGTTGGCCTCACTCTAGTCGCCCGGCCTGTGACCACAAGACGAACCCTGTGTG
  CTTCCTGGTCAGACAGTGACCTGTTCTCCAGGGACCAGAAAAGTCTGCCGCCCTCTCCCAACCAGTCCCA
  GCTGCTGGAGGAATTCCTGGATAACTTTAGAAAAACCGCCACGAGTGATTTCAGCAATGTCCCTGAGATC
  ACAACTGGCTTGAAGAGGAGCCAGACAGAAGGCACCCTGGATCAAGTGCCCCACAGGGAAAAGATGGAGC
  AGACATTCCTGAGCGCTGACCAGATTGCGGAGCTCTGCAGGGACTTGAACAACACCCACACCAACAGTAT
  GGAAGCACCAACAGAGAGCCATGACCCACCTCCCAGGCCTCTGGCTCGTCACCTCTCAGATGCAGATCGC
  CTCCGGAAAGTCATCCAGGAGCTTGTAGACACAGAGAAGTCTTACGTGAAGGATCTGAGCTGCCTCTTTG
  AACTATACTTGGAGCCACTTCAGAATGAGACCTTTCTTACCCAAGATGAGATGGAGTCACTTTTTGGGAG
  CCTGCCAGAGATGCTGGAGTTTCAAAAGGTGTTCCTGGAGACGTTGGAGGATGCGATCTCCGCTTCCTCG
  GACTTTAGTGTCCTGGAAACCCCCTCACAGTTTCGAAAATTGCTGTTCTCCCTTGGAGGTTCTTTCCTCT
  ACTATGCGGATCACTTTAAGCTATACAGTGGGTTCTGTGCCAACCACATTAAAGTACAGAGGGTTCTAGA
  GCGAGCTAAAACGGACAAGGCCTTCAAGGCTTTTCTGGATGCCCGAAATCCCACCAAGCAGCACTCCTCC
  ACGCTGGAGTCCTATCTCATCAAGCCTGTTCAGAGAGTGCTCAAGTATCCTCTGCTTCTCAAGGAGCTAG
  TGTCACTGACTGACCATGAGAGTGAAGAACACTATCACCTGACAGAAGCACTAAAGGCCATGGAAAAAGT
  CGCCAGTCACATCAATGAGATGCAGAAGATCTACGAGGATTACGGGATGGTGTTTGACCAGCTGGTGGCA
  GAGCAGAGTGGCACAGAGAAGGAAGTGACAGAGCTGTCCATGGGGGAACTTCTGATGCACTCTACAGTTT
  CCTGGTTGAATCCGTTCCTGTCTCTAGGAAAAGCCAGGAAGGACATTGAGCTCACAGTATTTGTTTTTAA
  GAGAGCTGTCATACTGGTTTATAAAGAAAACTGCAAGCTGAAAAAGAAACTGCCCTCGAATTCCCGGCCT
  GCTCACAACTCTGCTGACTTGGATCCATTTAAATTCCGCTGGTTGATTCCCATATCTGCGCTTCAAGTTA
  GACTGGGGAACACGGCAGGGACTGAAAATAATTCCACGTGGGAGCTGATTCATACCAAGTCGGAAATTGA
  AGGACGGCCAGAAACCATCTTTCAACTGTGCTGCAGTGACAGCGAGAACAAAACCAGCATTGTTAAGGTG
  ATTCGTTCTATTCTGAGAGAGAACTTCCGGCGCCACATAAAGTGTGAGCTGCCACTGGAGAAGACGTGTA
  AGGACCGGCTAGTACCTCTTAAGAACCGAGTTCCTGTTTCAGCCAAATTAGCCTCGTCCAGGTCGTTGAA
  GGGCCTCAGAACATCCTCCAGCAGCGAGTGGCCCAGCGAGCCCAGCAAGGGCAACTCACTGGACTCAGAT
  GAGTGCAGCCTGAGCAGTGGCACCCAGAGTAGCGGCTGCCCCGTAGCCGAGAGCAGGCGAGACTCTAAGA
  GCACCGAGCTGGAGAAAGACGCTCAGGAGGGCCTGGCGGAGTTTCCAGATGGTCTTATCAAAGAAAGCGA
  CATTCTGAGTGATGAAGATGAGGACTTCCACCACCCTCTGAAACAGGGTAGCCCTACTAAGGACATTGAG
  ATTCAGTTCCAGAGACTGAAAATCTCTGAGGAATCCGACGTGCACCCAGTTGGGCAGCAGCCTCTCACAG
  AGTCAGGTGAACAGCCCAAGCTGGTCAGGGGCCATTTTTGCCCCATTAAACGGAAAGCAAACAGCACCAA
  GAGGGGCAGAGGAACTTTGCTCAAGGCGCAGACTCGTCACCAGTCCCTGGACAGCCACCCAGAAACTGCC
  AGCATTGATCTAAACTTGGTCCTGGAGAGAGAATTCAGTGTCCAGAGCTTAACTTCAGTCGTCAATGAGG
  AGGGTTTTTATGAAACACAGAGCCATGGCAAATCATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_011878
• Insert Size: 5148 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011878.2, NP_036008.2
• RefSeq Size: 6128 bp
• RefSeq ORF: 5148 bp
• Locus ID: 24001
• UniProt ID: Q6ZPF3
• Cytogenetics: 17 1.99 cM
• Gene Summary: Modulates the activity of RHO-like proteins and connects extracellular signals to cytoskeletal activities. Acts as a GDP-dissociation stimulator protein that stimulates the GDP-GTP exchange activity of RHO-like GTPases and activates them. Activates specifically RAC1, but not CDC42 and RHOA. Mediates extracellular laminin signals to activate Rac1, contributing to neurite growth. Involved in lamellipodial formation and advancement of the growth cone of embryonic hippocampal neurons. Promotes migration of neurons in the cerebral cortex. When overexpressed, induces membrane ruffling accompanied by the accumulation of actin filaments along the altered plasma membrane.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) and variant 2 encode the same isoform (1).