Arhgef28 (NM_012026) Mouse Untagged Clone

CAT#: MC224839

Arhgef28 (untagged) - Mouse Rho-guanine nucleotide exchange factor (Rgnef), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Arhgef28
• Synonyms: 9230110L08Rik; AI323540; D13Bwg1089e; p190RhoGEF; Rgnef; RhoGEF; Rhoip2; RIP2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224839 representing NM_012026
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTTGAGCTGCAGTGAAGTGCCTCTTTATGGACAGAAGACGGTCTATGCAAAGTTTGGCAAAAACG
  TGTATCTACCCGAAGATGCGGAGTTTTACTTTGTTTATGGCGGGTCGCATCAGAGGCACGTGGTGATTGC
  TGACCGTGTCCAGGACAATGTCCTCCAATCTAGCATTCCAGGCCACTGGCTTCAGGAGACGGTGACGGTA
  TCTGTGTGCCTCTGCTCGGAAGGGTACTCCCCAGTGACCATGGGCTCTGGCTCAGTGACCTACGTGGACA
  ACATGGCCTGCAGGCTGGCTCGTCTGCTGGTCACTCAGGCTGACCGACTCACGGCCTGCAGTCACCAGAC
  GCTGCTGACCCCGTTTGCCCTGACAGTGGAAGCGCTGCCTGCCTTGGATGAGGAGCTCGTGCTGGCTCTG
  ACCCAACTGGAGCTGCCTTTGGGTTGGACAGTCCTGGGAAATTCCTCACTTGAAGTATCTCTACACAGAG
  AGTCTCTCCTGCATCTGGCTGTGAGATGGGCCCTCCCTAAGCTATTCCACTTCCTCTTGTGTCTCCCTGG
  AGGAGTCAAGGCCTTGAAGCTACCTAATGAAGAGGCCACCACACCATTAGACTTAGCTTTGCAAGGTGGA
  CACTCCACACTGGTAGAAGACATCACAAATTTTCAGGGTAGCCATTCCCCAGGCTTCTCCCGACTGCGTC
  TTAACGAAGAGGCCACTCTGCAGTTTGTTCATTCATCGGAAACATTGACCCTGACCGTTAACCACACAGC
  TGAGCATCTCTTGGAAGCAGACATCAAACTCTTCCGGAAATATTTCTGGGACAGAGCCTTTCTTGTCAAG
  GCTCTTGAGCAAGAAGCCAAGACTGAGAAAGCAACCATGCCTTCTGGAGCTGCAGAAACTGAAGAAGAGG
  TTAGGAATTTGGAGTCTGGCAGATCACCATCGGAGGAGGAGGAGGATGCAAAGAGCATCAAAAGCCAGGT
  GGATGGGCCGAGTGAACACGAAGACCAGGACCGCCTACCTCTGGATCGCTCCTTTGATGGCCTCAAAAAA
  TCCAAGCATGTCCCAGCGTCCCTTGCTGCAGGCCAGCTCTCTGATGTTCTGAACGGGGGTGATGAAGTCT
  ACGCTAACTGTATGGTGATTGACCAGGTTGGCGATTTGGATATCAACTATATAAACCTAGAGGGACTCTC
  CACCCATACGAGTCCCGAGTCTGGGAGGTCCATGCTGGGGCCTCAGGCTTGCATGCACACCCTTCCTCCC
  GACACCAGTCCCTGCGGGCGCCCACTCATTGAGAACAGTGAGGGGACCCTGGATGCTGCAGCTTCGCAGT
  CTTTCGTGACACCATCCAGTTCTCGCACTTCCAACTTGAACCTTTCTTTTGGTCTCCATGGATTTGAAAA
  GGAGCAGAGTCATCTCAAGAAAAGGAGTTCTAGCCTGGATGCCCTGGTTGCCGACAGTGAGGGGGAAGGA
  GGTTCTGAGCCCCCCATCTGCTATGCTGTGGGCTCTCAGAGTTCCCCAAGAACTGGACTCCCCAGCGGGG
  ATGAACTGGACTCTTTTGAGACCAACACTGAACCAGACTGTAATATCTCCAGGACCGAATCACTCTCTCT
  GTCCAGTACTCTGCATTCAAAGGAGTCCTTGCTTTCTGGGATCCGGTCTCGCTCCTACTCCTGCTCATCA
  CCCAAAATCTCTTCAGGAAAATCCCGGCTAGTGCGCGACTTCACAGTGTGCAGTACCAGCGAAGAACAGA
  GATCATATAGTTTCCAGGAGCCACCAGGAGAAAAAAGGATTCAGGAAGAAGAATGGGATGAATACGTCAT
  TCCTGCCAAATCCGAGTCTGAAAAGTACAAAGTGAGCCGAACTTTCAGCTTCCTCATGAACAGGATGACC
  AGCCCACGGAACAAATCTAAGATGAAAAACAAGGACACTAAAGAAAAAGAAAAAATGAATCGACATCAGT
  TTGTCCCGGGAACGTTCTCCGGGGTTCTGCAGTGCTCCGGTTGTGATAAAACACTCCTGGGGAAAGAGTC
  ACTCCAGTGTGCCAACTGTAAGGCAAACACACACAAGGGCTGCAAAGATGCTGTGCCTCCGTGTACCAAG
  AAATTCCAAGAGAAATACAACAAGAACAAACCACAGAGCATCCTTGGAAGCTCTTCAGTCAGAGACGTCC
  CAGCACCTGGGCTCTCCTTGCACCCTTCTTCCTCTATGCCCATCGGACTGCCAGCTGGAAGGAAGGAATT
  TGCGGCTCAGGTGCATCCACTGTCCAGAAGTGTCCCCGGCACCACTTTGGAAAGCTTCAGGAGGGCAGTG
  ACATCGTTAGAGTCTGAGGGTGACAGCTGGAGAAGCAGGTCTCATTCCGATGAGCTGTTCCAGTCTATGG
  GCTCTTCTCCTTCCACGGAGTCATTCATGATGGAAGATGTTGTGGATTCTTCTCTGTGGATCGACCTCAG
  TAGTGATGCTCAGGAGTTTGAAGCAGAGTCTTGGAGTCTCGTGGTGGACCCTTCCTTTTGCAGTAGGCAG
  GAGAAGGATGTCATCAAAAGACAAGATGTCATTTTTGAGCTGATGCAGACAGAGGTCCACCACATCCAGA
  CTCTGCTCATCATGTCCGAGGTCTTCAGGAAAGGCATGAAGGAGGAACTGCAGCTGGACCACAGCACGGT
  GGACAAGATCTTCCCCTGCTTAGATGAGCTGCTGGAGACTCACCGGCACTTCTTCTTCAGCATGAAGGAG
  CGCAGGCAGGAGTCCTGCGCTGGCAGTGACAGGAACTTTGTGATCAACCAAATTGGAGACATTCTGGTAC
  AACAGTTTTCAGAGGAAAATGCAAGCAAAATGAAGAGGATCTATGGAGAATTCTGCAGCCATCACAAAGA
  AGCCATGAGTCTCTTTAAAGAGCTGCAGCAGAACAAGAAGTTTCAGAATTTTATTAAGATCCGAAATAGC
  AACCTGTTGGCTCGTCGCCGCGGAATCCCGGAGTGCATCCTGCTAGTTACCCAGCGCATCACAAAGTACC
  CAGTCTTGGTGGAAAGGATCCTGCAGTACACCAAGGAAAGGACTGAAGAGCACCGGGACCTGTGCAAAGC
  TCTCGGCTTAATTAAAGACATGATCGCAGCCGTGGACCTGAAAGTCAGCGAATACGAGAAGAACCAAAAA
  TGGCTCGAGATCCTAAATAAAATCGAGAACAAAACATACACAAAGCTCAAAAATGGCCACGTGTTTAGAA
  AGCAGGCGCTGCTGAGCCAGGAACGGGCTCTCCTGCATGATGGGCTTGTCTACTGGAAAACCGCTACGGG
  TCGCTTCAAAGACATCCTGGCTCTGCTACTAACTGACGTGCTGCTCTTCTTACAAGAAAAAGACCAGAAA
  TACATCTTTGCAGCTGTTGACCAGAAGCCATCAGTGATTTCCCTCCAGAAGCTGATTGCTAGAGAAGTTG
  CCAACGAGGAGAGAGGCATGTTTCTGATCAGTGCTTCGTCAGCCGGCCCCGAGATGTACGAAATCCACAC
  CAATTCCAAGGAGGAGCGGAATAACTGGATGAGGCGGATCCAGCAGGCAGTAGAAAGTTGTCCAGAGGAA
  GAAGGAGGAAGGACGAGCGAGTCGGATGAAGAGAGGCGGAAAGCTGAAGCTAGAGTGGCCAAGATTCAGC
  AATGTCAAGAAATACTCAGTAACCAAGACCAACAGATTTGCACGTACTTGGAAGAAAAGCTGCATATCTA
  CGCTGAACTTGGGGAACTGAGTGGCTTTGAGGATGTCCACCTAGAACCCCATCTGCTCATCAAACCTGAC
  CCTGGAGAGCCTCCCCAGGCAGCGTCCCTGCTTGCAGCAGCCCTGAGAGAAGCCGAGAGTCTGCAAGTGG
  CTGTGAAGGCTTCAAAGATGGGCGATGTAAGCCAGTCATCCGAAGAAAGCCCTGGAGGGACTGTCCTGAT
  GGACACACCCAGTACACAGGATGTACCAGCGTCACCAACTGCTTCATTAGTCACAGAAGGGACAGAAGGA
  AGAGGCTGTTGGGATGTGGATCCCGGGCTCCAGGGTGTGGTAACCGACTTGGCAGTCTCTGATGCAGGGG
  AGAAGGTGGAATATAGAAGTTTCTCCGGTTCTTCACAGTCAGAGATTATACAAGCAATACAGAATTTAAC
  GCGTCTCCTCTACAGCCTTCAGGCTGCCTTGACCATCCAGGACAGTCACATTGAGATCCACAAACTGGTT
  CTACAGCAGCGGGAGAGCTTGGCCCCTAGCCACTCCTTCCGAGGTGGCCCCTTGCAAGACCAGGAGAAAT
  CCCGCTACCTGGAAAAGCAGCGTGAAGAGCTGGCCAACATCCACAAGCTTCAACACCAGTTCCAGCAGGA
  GCAGCGGCGCTGGCACCGCACGTGCGACCAGCAACAGCGCGAGCAGGAAGCCCAAGAGAGCTGGCTGCAG
  GCGCGGGAACGTGAGTGCCAGTCCCAGGAGGAATTGTTGCTGCGCCACCGTAGCGAGCTGGACCACCAAC
  TCCAGGAGTACCAGCAGAGCCTTGAGCGGCTACGGGAGGGCCAGCGGATGGTGGAGAGGGAGAGACAGAA
  GATGCGTGTCCAGCAGGGTCTGCTGGGCCACTGCAAGCATAGTCGCCAGCGGAGCCTCCCAGCCGTCTTC
  TCTCCAGGGAGCAAGGAGGTAACAGAACTTAACCGAGCTGAGAGTTTGTGTCATGAAAATTCATTTTTCA
  TCAATGAAGCTTTTGGACACATGTCACTTAACACGTCCAATAAACCAAACCCATCAGGGGTCCCCTGGGA
  TGCTCACCCCCTGGAGGGGTCTCATTTTGATTTGGCAAGGACTAGTGAGAGTCCAACAGAACTGAAGATA
  GACATTTCTCAGCCTCCTGATGTCAACTCTGAACTCTGGACAACGGGGCCGGGCCATCAGAGACCTGCTC
  TCCAAGAAAACAGCAAGGAGTCTTATAAAAATGTTGCAGATTTGGACTCCTTCCAGAGTGAATCGTCATC
  TCCCCAGGACTCCAATCAGCGTGGCCCCCAGCCACAGACACTCACAACAGAAGCAAAACTAAGTCTTCCG
  ATGGCGGCTGGACACGGTGGCGATGCTGGAGACGGGGCTGAAGAAAACATTCTTTACCTCTGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_012026
• Insert Size: 5103 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012026.2, NP_036156.2
• RefSeq Size: 6259 bp
• RefSeq ORF: 5103 bp
• Locus ID: 110596
• UniProt ID: P97433
• Cytogenetics: 13 51.6 cM
• Gene Summary: Functions as a RHOA-specific guanine nucleotide exchange factor regulating signaling pathways downstream of integrins and growth factor receptors. Functions in axonal branching, synapse formation and dendritic morphogenesis. Functions also in focal adhesion formation, cell motility and B-lymphocytes activation. May regulate NEFL expression and aggregation and play a role in apoptosis.[UniProtKB/Swiss-Prot Function]