Col4a4 (NM_007735) Mouse Untagged Clone

CAT#: MC224813

Col4a4 (untagged) - Mouse collagen, type IV, alpha 4 (Col4a4), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Col4a4
• Synonyms: E130010M05Rik; [a]4(IV)
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224813 representing NM_007735
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGTGCTTTTTCAGATGGACCAAGTCCTTTGTCACAGCCCCCTGGTCACTAATATTTATCCTCTTTA
  CTATACAATATGAATATGGGAGTGGCAAGAAGTATGGCGGCCCCTGCGGAGGAAGAAACTGCTCTGTCTG
  CCAGTGTTTTCCTGAGAAGGGATCTCGGGGTCACCCAGGACCACTGGGACCACAGGGACCAATAGGGCCC
  TTGGGACCCCTGGGACCTATTGGGATTCCAGGAGAAAAAGGAGAGAGAGGAGACAGTGGCTCACCTGGAC
  CACCAGGAGAGAAAGGAGACAAGGGTCCAACTGGTGTCCCTGGATTTCCAGGTGTGGATGGTGTACCTGG
  TCACCCAGGGCCTCCTGGACCCAGAGGCAAACCTGGTGTGGACGGCTACAATGGCTCACGTGGTGATCCG
  GGCTATCCAGGAGAAAGAGGAGCTCCAGGCCCAGGCGGCCCCCCTGGTCAGCCTGGGGAAAATGGAGAAA
  AAGGAAGATCTGTGTACATTACTGGTGGCGTTAAAGGTATTCAGGGAGACCGTGGGGACCCAGGACCACC
  CGGCTTACCAGGATCTAGGGGTGCACAAGGGTCACCAGGGCCCATGGGGCATGCAGGCGCACCAGGGCTA
  GCAGGACCTATAGGTCATCCTGGAAGCCCTGGGTTGAAGGGAAATCCTGCCACTGGACTGAAGGGACAAA
  GGGGAGAGCCGGGTGAAGTTGGCCAGCGTGGTCCTCCTGGGCCCACGCTGTTGGTACAGCCGCCTGATTT
  GAGTATCTATAAAGGAGAAAAGGGTGTAAAAGGAATGCCTGGAATGATTGGTCCCCCAGGACCTCCAGGT
  CGCAAGGGAGCGCCTGGTGTCGGGATCAAAGGAGAGAAAGGTATCCCTGGGTTCCCAGGACCCCGGGGTG
  AGCCTGGTTCGCATGGGCCTCCAGGTTTTCCAGGATTCAAGGGGATACAAGGAGCAGCTGGAGAGCCTGG
  GCTGTTCGGATTTCTTGGTCCAAAGGGGGATCTGGGAGATCGCGGGTACCCGGGACCACCAGGCATTTTG
  TTGACTCCAGCTCCACCACTCAAAGGTGTTCCAGGGGACCCAGGACCCCCTGGCTACTATGGGGAGATTG
  GAGATGTTGGATTACCAGGTCCTCCAGGGCCCCCAGGCAGACCAGGGGAAACTTGTCCAGGCATGATGGG
  ACCTCCTGGACCACCAGGGGTTCCTGGTCCTCCAGGATTTCCAGGAGAAGCTGGGGTTCCTGGAAGACTC
  GACTGTGCCCCAGGAAAACCTGGGAAGCCAGGGCTACCCGGCCTGCCTGGCGCACCAGGACCAGAGGGGC
  CCCCTGGATCAGATGTGATATACTGTCGCCCTGGGTGCCCTGGGCCAATGGGTGAAAAGGGAAAAGTGGG
  TCCTCCAGGAAGAAGAGGAGCCAAAGGAGCAAAAGGAAACAAGGGACTCTGTACCTGTCCACCTGGTCCC
  ATGGGACCCCCTGGTCCCCCAGGACCTCCTGGAAGACAAGGAAGTAAAGGAGACTTAGGGCTCCCTGGTT
  GGCATGGAGAAAAGGGCGATCCAGGTCAGCCTGGTGCTGAAGGACCGCCAGGGCCACCAGGAAGACCTGG
  TGCCATGGGGCCCCCTGGTCACAAAGGGGAAAAGGGTGACATGGTCATATCAAGAGTGAAAGGGCAAAAA
  GGAGAAAGAGGACTGGATGGGCCACCAGGATTTCCGGGGCCACATGGACAAGATGGTGGGGATGGACGTC
  CTGGAGAAAGAGGGGATCCTGGGCCACGAGGGGACCATAAGGATGCAGCCCCGGGTGAGAGAGGGCTTCC
  CGGACTGCCAGGCCCTCCAGGAAGAACAGGACCTGAGGGGCCTCCAGGCCTGGGATTTCCAGGCCCACCA
  GGACAGCGAGGCCTACCCGGAGAGCCCGGGCGTCCGGGCACCAGAGGCTTTGATGGCACGAAGGGGCAGA
  AAGGTGACTCCATTCTGTGTAATGTAAGCTACCCGGGAAAACCAGGTCTGCCAGGTCTTGATGGACCTCC
  AGGTCTGAAGGGGTTTCCAGGCCCCCCAGGGGCTCCTGGTATGAGATGTCCAGATGGGCAGAAAGGCCAG
  CGGGGCAAACCGGGAATGTCTGGGATTCCAGGTCCACCTGGTTTTCGTGGTGACATGGGAGATCCAGGCA
  TCAAAGGTGAAAAGGGGACCTCCCCTATTGGACCCCCAGGCCCACCTGGGTCTCCTGGAAAGGATGGCCA
  GAAAGGAATCCCAGGAGACCCTGCATTTGGTGACCCAGGACCCCCTGGAGAGAGGGGTCTTCCAGGAGCA
  CCAGGCATGAAAGGACAGAAAGGTCACCCAGGGTGCCCAGGGGCTGGAGGCCCTCCTGGCATCCCTGGAT
  CTCCAGGTCTCAAAGGTCCCAAAGGTAGAGAGGGAAGTCGTGGCTTTCCAGGGATCCCAGGATCCCCTGG
  CCATTCCTGTGAAAGAGGTGCTCCCGGGATCCCAGGACAACCGGGACTTCCTGGAACTCCAGGTGATCCA
  GGGGCCCCAGGTTGGAAAGGCCAGCCAGGAGACATGGGTCCCTCTGGACCAGCTGGGATGAAGGGCCTCC
  CTGGTCTCCCAGGCCTACCAGGAGCTGATGGTCTCCGAGGGCCTCCTGGGATTCCAGGCCCCAATGGGGA
  AGATGGTCTACCTGGTCTTCCAGGCCTGAAGGGACTCCCAGGGCTGCCTGGCTTCCCTGGTTTTCCAGGA
  GAGAGAGGAAAGCCAGGGCCAGATGGAGAACCTGGAAGAAAGGGAGAGGTTGGAGAGAAAGGCTGGCCTG
  GCTTGAAGGGAGACCTGGGAGAGCGAGGCGCCAAAGGAGACAGAGGACTTCCTGGTGATGCAGGAGAAGC
  AGTCACTTCCAGAAAGGGTGAACCTGGGGATGCTGGGCCTCCAGGAGATGGTGGGTTCTCAGGAGAGAGA
  GGTGATAAAGGAAGCTCAGGGATGCGAGGGGGAAGAGGGGATCCCGGAAGAGATGGACTACCGGGACTCC
  ACAGAGGGCAGCCTGGGATAGATGGGCCTCCTGGGCCTCCTGGGCCCCCTGGCCCTCCAGGATCACCTGG
  ACTGAGAGGAGTCATTGGTTTCCCAGGATTTCCAGGTGACCAGGGTGATCCAGGTTCTCCAGGACCCCCT
  GGATTCCCAGGAGATGATGGAGCAAGAGGACCTAAAGGATACAAAGGTGACCCTGCCAGTCAGTGTGGTC
  CACCTGGTCCAAAGGGTGAGCCAGGTAGTCCTGGATACCAAGGACGTACTGGAGTCCCCGGAGAGAAAGG
  CTTTCCTGGAGATGAAGGACCCCGAGGACCACCAGGCAGACCTGGACAGCCTGGATCTTTTGGACCACCA
  GGGTGTCCAGGTGACCCAGGGATGCCCGGACTCAAGGGACATCCAGGAGAAGTGGGAGACCCTGGGCCAA
  GAGGTGATGCGGGAGATTTCGGGAGGCCAGGTCCTGCTGGAGTGAAAGGGCCTCTCGGGTCTCCTGGTCT
  GAATGGCTTACATGGTCTGAAGGGTGAGAAAGGAACAAAAGGCGCCTCAGGTTTGCTCGAAATGGGTCCA
  CCTGGGCCAATGGGGATGCCTGGGCAAAAAGGAGAGAAAGGAGACCCTGGGAGTCCAGGGATTTCTCCTC
  CAGGTCTTCCTGGAGAAAAGGGCTTCCCAGGACCCCCAGGGAGACCAGGACCACCTGGTCCCGCAGGTGC
  CCCAGGAAGAGCTGCTAAAGGTGACATTCCTGATCCAGGTCCACCTGGAGACCGGGGACCTCCTGGCCCC
  GATGGCCCAAGAGGAGTACCTGGGCCTCCGGGTTCCCCTGGGAATGTTGACCTTCTGAAAGGGGACCCAG
  GTGACTGTGGTTTGCCAGGACCACCTGGCTCCCGAGGCCCACCAGGCCCTCCAGGGTGTCAGGGTCCCCC
  AGGATGTGATGGCAAAGATGGCCAGAAAGGACCAATGGGACTTCCAGGGTTGCCAGGGCCACCTGGTCTT
  CCTGGGGCACCTGGCGAGAAGGGATTACCTGGCCCTCCAGGCAGAAAAGGGCCAGTAGGTCCTCCAGGCT
  GCAGAGGTGAACCTGGGCCTCCTGCAGACGTGGATTCCTGTCCTCGAATCCCAGGACTTCCTGGAGTGCC
  AGGTCCCAGAGGACCAGAAGGAGCTATGGGGGAACCTGGACGGAGAGGCCTGCCTGGACCAGGGTGCAAA
  GGAGAGCCTGGACCGGATGGCAGACGGGGCCAGGATGGCATCCCTGGATCTCCCGGGCCTCCTGGACGCA
  AAGGTGACACTGGAGAGGCAGGGTGTCCTGGAGCACCAGGCCCTCCTGGTCCAACTGGTGATCCTGGGCC
  CAAGGGGTTTGGCCCTGGATCGCTCAGTGGCTTCCTCCTGGTTCTCCACAGTCAGACTGACCAGGAACCG
  GCCTGCCCCGTGGGCATGCCTCGGCTTTGGACAGGGTACAGCTTGTTGTACATGGAAGGACAGGAGAAGG
  CGCACAATCAAGATCTTGGTTTGGCAGGCTCTTGTCTTCCTGTGTTTAGCACTCTGCCCTTTGCCTACTG
  CAACATCCACCAAGTGTGCCACTACGCCCAGAGAAATGACAGGTCCTACTGGCTGTCCAGTGCGGCTCCT
  CTTCCTATGATGCCACTCTCAGAGGAAGAGATCCGCTCTTACATCAGCCGCTGTGCGGTGTGTGAGGCCC
  CAGCACAGGCCGTGGCGGTCCACAGTCAGGACCAATCCATCCCACCGTGTCCACGGACATGGAGGAGTCT
  CTGGATTGGGTACTCATTTCTGATGCACACTGGAGCTGGGGACCAAGGAGGTGGGCAGGCACTGATGTCA
  CCTGGCAGCTGCCTAGAGGACTTCAGAGCTGCACCGTTCGTTGAATGCCAGGGCCGACAGGGAACCTGTC
  ACTTTTTTGCAAACGAGTACAGCTTCTGGCTGACCACCGTGAACCCAGACCTGCAGTTTGCCTCTGGCCC
  GTCACCAGACACCTTGAAAGAAGTTCAGGCCCAGCGCCGGAAAATCAGCAGGTGCCAGGTTTGCATGAAG
  CACAGCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_007735
• Insert Size: 5049 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007735.2, NP_031761.1
• RefSeq Size: 7808 bp
• RefSeq ORF: 5049 bp
• Locus ID: 12829
• UniProt ID: Q9QZR9
• Cytogenetics: 1 C5
• Gene Summary: Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.[UniProtKB/Swiss-Prot Function]