Col4a1 (NM_009931) Mouse Untagged Clone

CAT#: MC224806

Col4a1 (untagged) - Mouse collagen, type IV, alpha 1 (Col4a1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Col4a1
• Synonyms: Br; Bru; Col4; Col4a-1; Del(8)44; R; Raw; S; Svc
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224806 representing NM_009931
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCCCCGGCTCAGCGTCTGGCTTCTGCTGCTCTTCGCCGCCCTTCTGCTCCACGAGGAGCGCAGCC
  GAGCAGCTGCGAAGGGCGATTGTGGTGGCTCTGGCTGTGGAAAATGTGACTGTCATGGCGTGAAGGGACA
  AAAGGGAGAAAGAGGCTTGCCAGGGTTGCAAGGTGTCATTGGATTTCCCGGGATGCAAGGACCTGAGGGG
  CCACATGGACCACCAGGACAAAAGGGTGATGCTGGAGAACCTGGACTTCCTGGCACAAAAGGGACGAGGG
  GACCCCCTGGAGCAGCTGGCTACCCTGGGAATCCCGGACTTCCTGGTATTCCTGGCCAAGATGGCCCTCC
  AGGTCCCCCAGGTATCCCAGGATGCAACGGTACAAAGGGAGAGAGAGGGCCGCTCGGTCCTCCTGGCTTG
  CCTGGATTCAGCGGCAATCCTGGACCACCAGGGTTACCAGGAATGAAGGGAGATCCTGGTGAAATTCTCG
  GCCACGTTCCCGGAACACTGCTGAAAGGGGAGAGAGGATTTCCTGGTATCCCCGGGATGCCGGGCTCACC
  AGGGTTGCCAGGACTCCAGGGACCCGTGGGTCCTCCGGGATTTACTGGACCACCGGGTCCTCCAGGCCCT
  CCTGGACCTCCTGGAGAAAAGGGCCAGATGGGCTCCAGCTTCCAAGGACCAAAAGGTGACAAGGGAGAGC
  AAGGGGTCAGCGGTCCCCCGGGAGTTCCTGGACAGGCACAAGTTAAGGAAAAAGGAGACTTTGCCCCAAC
  AGGAGAAAAGGGTCAGAAAGGTGAACCTGGATTTCCGGGAGTACCAGGATATGGAGAGAAAGGTGAACCT
  GGCAAGCAAGGGCCTCGGGGAAAACCTGGGAAAGACGGTGAAAAAGGAGAAAGGGGGAGCCCGGGCATTC
  CTGGCGATTCCGGGTACCCAGGTCTCCCAGGCCGGCAGGGCCCGCAGGGAGAGAAGGGTGAAGCTGGACT
  TCCAGGCCCCCCTGGAACTGTGATAGGCACCATGCCTTTGGGAGAGAAAGGAGATCGAGGCTACCCAGGA
  GCACCAGGGTTGAGAGGAGAGCCCGGCCCTAAAGGTTTCCCTGGAACACCAGGCCAACCAGGCCCTCCAG
  GTTTCCCTACTCCAGGCCAGGCTGGTGCTCCAGGCTTCCCAGGTGAAAGGGGAGAAAAAGGTGACCAGGG
  ATTTCCAGGCGTGTCATTGCCAGGACCAAGTGGAAGAGATGGAGCCCCGGGGCCTCCCGGCCCTCCCGGC
  CCCCCTGGGCAGCCAGGCCACACAAATGGCATTGTGGAGTGTCAACCTGGACCACCCGGGGACCAGGGCC
  CTCCTGGGACTCCAGGACAGCCAGGTTTGACAGGTGAAGTTGGACAGAAAGGTCAGAAAGGAGAGAGCTG
  CCTTGCCTGTGACACAGAAGGACTTCGTGGCCCTCCAGGGCCACAGGGGCCTCCGGGAGAGATTGGTTTC
  CCTGGACAACCTGGGGCTAAAGGTGACCGAGGCCTGCCCGGCAGAGATGGTCTTGAAGGATTGCCGGGTC
  CACAAGGTTCACCAGGGCTTATAGGCCAGCCTGGAGCTAAGGGAGAGCCTGGAGAGATATTTTTTGACAT
  GCGACTCAAAGGTGACAAAGGAGACCCAGGTTTTCCGGGACAGCCTGGCATGCCAGGAAGAGCAGGAACT
  CCCGGAAGAGATGGCCACCCAGGACTCCCTGGACCCAAAGGCTCTCCGGGTTCAATAGGATTGAAAGGAG
  AGCGAGGTCCTCCCGGAGGAGTTGGCTTCCCTGGTAGTCGTGGAGACATCGGCCCTCCTGGACCCCCAGG
  AGTTGGCCCCATTGGTCCTGTTGGTGAAAAAGGACAAGCAGGCTTTCCTGGAGGCCCTGGGTCCCCAGGT
  CTTCCAGGCCCAAAGGGTGAGGCAGGAAAGGTTGTCCCACTCCCTGGCCCCCCAGGAGCTGCAGGACTTC
  CAGGATCCCCTGGCTTCCCAGGGCCACAAGGTGACCGAGGCTTCCCAGGAACCCCAGGACGTCCAGGCAT
  CCCGGGAGAGAAAGGTGCTGTGGGCCAGCCAGGAATTGGATTTCCTGGGCTTCCTGGCCCCAAAGGTGTT
  GATGGCTTGCCTGGAGAGATAGGACGGCCTGGGAGTCCAGGTCGCCCTGGATTTAACGGCTTACCTGGCA
  ACCCAGGACCTCAAGGTCAAAAGGGAGAACCTGGCATTGGGCTTCCAGGACTCAAAGGGCAACCAGGCCT
  TCCAGGCATTCCCGGTACACCTGGAGAGAAGGGTAGCATCGGGGGACCCGGCGTTCCAGGAGAACAGGGG
  TTGACAGGCCCCCCAGGACTCCAGGGGATCAGAGGTGACCCAGGGCCTCCTGGAGTTCAAGGCCCAGCAG
  GTCCACCAGGGGTCCCAGGAATAGGGCCACCCGGAGCTATGGGCCCTCCCGGAGGGCAAGGACCACCAGG
  GTCATCAGGTCCACCTGGAATTAAAGGAGAGAAAGGGTTCCCTGGATTCCCCGGACTGGATATGCCTGGC
  CCCAAAGGCGATAAAGGCTCTCAAGGACTTCCTGGCCTCACAGGACAGTCAGGCCTCCCTGGCCTTCCTG
  GACAGCAGGGGACACCTGGAGTTCCAGGGTTCCCAGGTTCTAAAGGTGAAATGGGTGTCATGGGAACCCC
  GGGACAACCAGGCTCGCCAGGACCAGCAGGCACCCCAGGGTTACCTGGAGAAAAAGGGGACCATGGCCTT
  CCGGGCTCCTCAGGACCCAGGGGCGACCCTGGCTTCAAAGGTGATAAAGGTGACGTTGGGCTTCCTGGCA
  TGCCAGGATCCATGGAGCATGTGGACATGGGGAGCATGAAGGGACAGAAAGGAGACCAGGGAGAGAAAGG
  ACAAATCGGACCCACTGGTGATAAAGGTTCCCGAGGAGACCCTGGAACACCAGGAGTACCTGGGAAGGAT
  GGGCAGGCAGGGCATCCCGGACAGCCAGGGCCTAAAGGTGACCCAGGCCTTAGTGGGACACCAGGATCCC
  CTGGACTCCCTGGACCCAAAGGATCAGTTGGAGGAATGGGCTTGCCAGGTTCGCCTGGAGAAAAAGGTGT
  GCCTGGCATCCCTGGCTCACAGGGTGTCCCTGGCTCACCTGGAGAGAAGGGAGCCAAAGGAGAGAAAGGG
  CAGTCAGGTCTACCTGGCATTGGGATTCCGGGACGGCCTGGTGACAAGGGAGACCAGGGCCTTGCTGGCT
  TCCCAGGCAGCCCCGGTGAGAAGGGAGAGAAAGGCAGTGCCGGAACCCCAGGGATGCCAGGGTCCCCAGG
  CCCGAGAGGTTCTCCGGGGAACATCGGCCATCCAGGAAGCCCAGGCCTGCCTGGAGAGAAAGGGGATAAA
  GGCCTCCCAGGACTGGATGGCGTTCCTGGTGTCAAAGGAGAAGCAGGTCTCCCTGGGACTCCTGGCCCCA
  CAGGCCCAGCTGGCCAGAAGGGAGAGCCGGGCAGCGATGGAATCCCGGGGTCGGCAGGAGAGAAGGGTGA
  ACAAGGTGTTCCAGGAAGAGGCTTCCCCGGCTTCCCTGGCTCCAAAGGAGACAAAGGCTCCAAGGGTGAA
  GTGGGTTTCCCTGGCCTAGCTGGAAGTCCTGGAATTCCTGGAGTCAAAGGCGAGCAAGGGTTCATGGGTC
  CTCCTGGCCCTCAAGGACAACCTGGCTTACCTGGCACTCCTGGTCACCCTGTGGAGGGGCCCAAAGGAGA
  CCGAGGACCTCAGGGTCAACCTGGCCTGCCAGGGCATCCGGGACCTATGGGGCCGCCAGGGTTCCCAGGA
  ATCAATGGGCCAAAAGGTGACAAGGGAAATCAAGGTTGGCCAGGAGCTCCGGGGGTTCCAGGCCCTAAGG
  GAGACCCAGGATTCCAAGGCATGCCGGGCATTGGCGGCTCTCCAGGGATCACAGGTTCAAAGGGAGATAT
  GGGACTGCCCGGAGTTCCAGGATTTCAAGGTCAGAAAGGGCTTCCTGGTCTGCAGGGAGTGAAAGGAGAC
  CAGGGAGACCAAGGTGTACCCGGCCCTAAAGGTCTCCAAGGTCCCCCTGGGCCTCCAGGTCCCTACGATG
  TCATCAAAGGAGAACCAGGGCTCCCTGGTCCTGAGGGTCCCCCTGGTCTTAAAGGACTCCAGGGACCACC
  AGGTCCAAAAGGACAGCAAGGTGTGACAGGCTCAGTGGGCTTGCCTGGACCTCCAGGTGTCCCTGGGTTC
  GATGGTGCTCCTGGCCAGAAAGGAGAGACAGGACCCTTTGGACCACCTGGTCCAAGAGGGTTTCCTGGCC
  CACCAGGCCCCGATGGGCTGCCAGGATCCATGGGTCCCCCAGGTACCCCATCTGTGGACCATGGCTTCCT
  TGTGACCAGGCATAGTCAGACAACAGATGACCCACTGTGTCCCCCAGGGACCAAAATTCTTTACCATGGA
  TACTCTCTGCTCTATGTCCAAGGCAACGAGCGTGCCCACGGGCAGGACTTGGGTACGGCTGGCAGCTGCC
  TGCGTAAGTTCAGCACCATGCCCTTTCTCTTCTGCAACATCAACAACGTCTGCAACTTCGCCTCCAGGAA
  CGACTACTCTTACTGGCTGTCCACGCCAGAGCCCATGCCCATGTCCATGGCACCCATCTCTGGGGACAAC
  ATCCGGCCCTTCATTAGCAGGTGTGCGGTTTGTGAAGCACCGGCCATGGTGATGGCGGTACACAGTCAGA
  CCATTCAGATTCCGCAGTGCCCTAACGGTTGGTCCTCACTGTGGATCGGCTATTCCTTCGTGATGCACAC
  CAGCGCTGGTGCTGAAGGTTCCGGCCAAGCCCTCGCATCCCCCGGGTCCTGTCTGGAAGAGTTTAGAAGC
  GCCCCATTCATCGAGTGCCACGGCAGAGGAACGTGCAATTACTACGCAAATGCTTACAGCTTTTGGCTCG
  CCACCATAGAGAGAAGCGAGATGTTCAAGAAGCCCACGCCATCCACCTTGAAGGCAGGGGAGCTGCGAAC
  ACACGTCAGCCGCTGCCAAGTGTGCATGAGAAGAACATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009931
• Insert Size: 5010 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009931.2, NP_034061.2
• RefSeq Size: 6636 bp
• RefSeq ORF: 5010 bp
• Locus ID: 12826
• UniProt ID: P02463
• Cytogenetics: 8 5.53 cM
• Gene Summary: This gene encodes the alpha-1 subunit of the type IV collagens, an essential component of basement membranes. The encoded protein forms a triple helical heterotrimer comprised of two alpha-1 and one alpha-2 subunits that assembles into a type IV collagen network. This gene is located adjacent to the gene encoding alpha-2 subunit. Mice lacking both the alpha-1 and alpha-2 subunits of collagen IV die in utero due to structural deficiencies in the basement membranes and certain mutations in this gene cause perinatal cerebral hemorrhage and porencephaly. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Nov 2015]
  Transcript Variant: This variant (1) represents the shorter transcript and encodes the functional protein.