Eif2ak4 (NM_013719) Mouse Untagged Clone

CAT#: MC224787

Eif2ak4 (untagged) - Mouse eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Eif2ak4
• Synonyms: 2610011M03; GCN2; MGCN2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224787 representing NM_013719
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGGGGGCCGCGGGGCCTCTGGACGCGGCCGGGCTGAGCCACAGGAGAGCTATTCGCAGCGACAGG
  ACCACGAGCTGCAGGCCCTGGAGGCCATTTACGGCTCGGACTTCCAGGATCTGCGGCCGGATGCGCGCGG
  ACGGGTGAGAGAGCCTCCTGAAATCAACTTAGTTCTTTACCCTCAGGGCCTAGCTGGTGAAGAAGTATAC
  GTGCAAGTGGAACTGAGGGTTAAATGCCCACCTACATACCCAGATGTAGTTCCCGAAATAGACTTAAAAA
  ATGCTAAAGGCCTGTCGAATGAAAGTGTTAATTTGTTAAAATCACACCTGGAAGAACTGGCCAAAAAGCA
  GTGTGGAGAGGTGATGATATTTGAACTGGCACACCATGTGCAGTCGTTTCTCAGCGAGCATAACAAGCCC
  CCACCAAAGTCTTTCCATGAAGAAATGCTGGAAAGGCAGGCTCAGGAGAAGCAGCAGAGGTTGCTGGAGG
  CCAGGCGGAAGGAGGAGCAGGAGCAACGTGAAATCCTCCACGAGATTCAGAGAAGGAAAGAGGAGATCAA
  GGAAGAGAAGAAAAGGAAGGAAATGGCTAAGCAGGAACGTTTGGAAATCACTAGCTTGACAAACCAGGAC
  TATGCCTCTAAGAGAGACCCAGCAGGGCACAGGGCAGCTGCCATCCTCCATGGAGGCTCTCCTGACTTTG
  TAGGAAATGGTAAAGCCCGGACATACTCCTCAGGAAGGTCCAGGCGAGAACGTCAGTATTCTGTGTGTAG
  TGGTGAACCCTCTCCTGGCTCGTGTGACATCCTCCATTTCTCTGTGGGTAGCCCTGATCAGCTCATGGTG
  CACAAAGGGAGATGTGTTGGCAGTGATGAGCAGCTGGGAAAGGTGGTATACAATGCTTTGGAAACAGCCA
  CGGGCAGCTTTGTCTTGCTGCATGAGTGGGTCCTTCAATGGCAGAAGATGGGCCCTTGCCTTACCAGTCA
  AGAGAAAGAGAAGATTGACAAGTGCAAAAGGCAGATTCAAGGAGCAGAAACAGAATTCAGCTCCCTAGTG
  AAACTGAGCCATCCAAATATCGTCCGCTACTTTGCGATGAACTCCAGAGAGGAGGAGGACTCCATTGTGA
  TCGACATTCTGGCAGAGCACGTCAGTGGCATCTCTCTGGCTACACACCTGAGCCACTCGGGCCCAGTCCC
  CGCCCACCAGCTGCGCAAGTACACAGCCCAGCTCCTGGCAGGCCTGGATTACCTACACAGCAACTCCGTG
  GTGCACAAGGTTCTGAGCGCGTCCAGTGTCTTGGTAGATGCCGAGGGCACTGTCAAGATAACAGACTACA
  GCATCTCTAAGCGTCTGGCAGACATTTGCAAGGAGGATGTATTTGAGCAAGCTCGAGTTCGTTTTAGTGA
  CAGTGCCCTGCCTTATAAAACAGGAAAGAAAGGGGATGTGTGGCGGCTCGGCCTCCTGCTGTTGTCTCTC
  AGCCAAGGACAGGAGTGTGGGGAGTATCCGGTGACCATCCCCAGTGACCTGCCAGCTGACTTCCAAGACT
  TCCTGAAGAAGTGTGTCTGCCTGGACGACAAGGAAAGATGGAGCCCTCAGCAGCTGCTGAAACACAGCTT
  TATAAACCCTCAACCAAAGCTGCCTTTGGTGGAGCAGAGTCCTGAAGACTCTGGGGGACAAGACTACATT
  GAGACCGTCATTCCCAGCAACCAGCTGCCCAGCGCTGCGTTCTTCAGTGAGACACAGAAACAGTTCTCCC
  GGTACTTCATTGAGTTTGAAGAACTACAACTCCTAGGGAAAGGAGCTTTTGGAGCTGTCATCAAGGTGCA
  AAACAAGCTTGATGGCTGCTGCTATGCTGTGAAGCGCATCCCTATCAACCCTGCAAGCAGACACTTCCGC
  CGGATCAAGGGCGAGGTGACACTGCTATCGCGCCTGCACCATGAGAACATTGTGCGCTACTACAACGCCT
  GGATCGAGCGGCATGAGCGCCCAGCGGTGCCAGGGACACCGCCCCCAGACTGCACACCCCAGGCCCAGGA
  CAGCCCAGCCACCTGCGGGAAAACATCAGGCGACACTGAAGAACTGGGCAGCGTGGAGGCTGCAGCACCG
  CCACCCATCCTCAGCAGCTCGGTGGAGTGGAGCACATCTGCAGAGCGTTCTACCAGCACCCGCTTCCCAG
  TCACGGGCCAGGATTCCAGCAGCGATGAGGAGGACGAGGACGAGCGCGATGGCGTCTTCTCCCAGTCCTT
  TCTACCTGCTTCAGATTCTGACAGTGACATCATCTTTGACAATGAAGATGAAAACAGTAAAAGTCAAAAT
  CAGGATGAAGACTGCAATCAAAAGGACGGTAGCCATGAGATTGAGCCTTCAGTGACAGCTGAGGCTGTGC
  ACTACCTTTATATCCAGATGGAGTACTGCGAGAAGAGCACGCTGCGGGACACCATTGACCAGGGGCTGTT
  CCGAGACACCAGCCGGCTCTGGAGGCTTTTCCGAGAGATTCTGGATGGATTAGCTTATATCCATGAGAAA
  GGAATGATTCATCGCGACTTGAAGCCTGTCAATATTTTTTTGGATTCTGATGACCATGTGAAAATAGGTG
  ACTTTGGCCTGGCAACAGACCATCTGGCCTTCACTGCTGAAGGTAAACAGGACGACCAGGCAGGTGACGG
  AGTGATTAAGTCAGACCCTTCAGGCCATTTGACTGGCATGGTTGGTACTGCTCTGTATGTAAGCCCTGAG
  GTCCAAGGAAGCACCAAGTCTGCATACAACCAGAAAGTGGATCTCTTCAGCCTGGGAATTATCTTCTTTG
  AGATGTCTTATCACCCGATGGTGACTGCCTCAGAAAGAATTTTCGTTCTCAACCAACTTCGAGATCCCAC
  ATCGCCCAAGTTTCCAGACGACTTCGATGATGGAGAACATACAAAGCAGAAATCTGTCATCTCCTGGCTG
  TTGAACCATGATCCAGCTAAACGGCCCACGGCCATGGAACTGCTCAAGAGTGAGCTGCTCCCCCCGCCGC
  AGATGGAGGAGTCCGAGCTGCATGAAGTGCTGCACCACACGCTGGCCAACATAGATGGGAAGGCCTACCG
  CACCATGATGAGTCAGATCTTCTGCCAGCACATCTCCCCCGCTATAGACTACACCTACGACAGTGACATC
  CTGAAGGGCAACTTCTTGATTCGTACAGCCAAGATCCAGCAGCTTGTATGTGAAACCATCGTCCGCGTCT
  TTAAAAGGCATGGCGCTGTCCAGCTGTGCACCCCACTGCTGCTTCCCCGAAACAGGCAAATATATGAGCA
  CAACGAAGCGGCTTTGTTCATGGATCACAGCGGCATGCTGGTGATGCTTCCGTTCGACCTGCGGGTCCCT
  TTTGCAAGATATGTGGCAAGAAATAACATACTGAATTTAAAACGATACTGCATAGAGCGGGTGTTCAGAC
  CTCGGAAGTTAGACCGATTTCATCCCAAAGAACTCCTGGAATGTGCATTTGATATCGTCACTTCTACCAC
  CAACAGCTCTCTGCCCACTGCCGAAACCATCTACACCATCTATGAAATAATCCAGGAGTTTCCTGCACTT
  CAGGAAAGGAATTACAGCATTTACTTGAACCATACGATGCTTCTGAAGGCAATACTCCTACACTGTGGGA
  TCCCAGAGGACAAGCTCAGCCAAGTCTACGTCATTCTGTATGATGCTGTGACAGAGAAGCTGACTAGGAG
  AGAAGTGGAAGCTAAATTCTGTAACCTGTCCTTGTCTTCTAATAGCCTGTGTAGACTCTACAAGTTCATT
  GAGCAGAAGGGGGATTTGCAAGACTTAACGCCAACCATCAACTCACTAATAAAACAGAAAACAGGCGTTG
  CTCAGTTGGTGAAGTATAGCTTAAAAGACCTCGAGGACGTCGTCGGGCTGCTGAAGAAACTTGGCGTGAA
  GTTACAGGTCTCCATCAACCTGGGCTTGGTCTACAAGGTGCAGCAGCACACTGGCATCATCTTCCAGTTC
  CTGGCATTCAGCAAACGCAGGCAAAGGGTTGTGCCTGAGATCCTTGCCGCCGGTGGCAGATACGACCTGC
  TGATTCCCAAGTTCAGAGGCCCACAGACTGTGGGGCCAGTCCCCACTGCTGTCGGTGTCAGCATAGCCAT
  AGACAAGATATTTGCTGCTGTCCTCAACATGGAAGAGCCTGTTACAGTGAGCTCCTGTGACCTCCTGGTT
  GTCAGTGTTGGCCAGATGTCCATGTCCAGGGCCATCAACCTAACCCAGAAACTGTGGACAGCGGGCATCA
  CTGCAGAAATCATGTATGACTGGTCCCAGTCCCAGGAAGAGTTACAAGAGTACTGCAGACACCATGAAAT
  CACCTATGTGGCTCTGGTCTCCGATAAAGAAGGAAGCCATGTCAAGGTCAAGTCCTTTGAGAAGGAGAGG
  CAAACAGAAAAGCGTGTATTGGAATCGGATCTTGTGGATCACGTTATGCAGAAACTAAGAACCAAAGTTG
  GTGACGAAAGAAATTTCAGAGACGCTTCTGATAATCTTGCCGTACAAACTCTGAAGGGGTCGTTTTCTAA
  TGCTTCAGGTTTGTTTGAAATCCATGGAACTACAGTAGTCCCCAACGTGATTGTTCTAGCACCAGAAAAG
  CTGTCAGCCAGCACCAGGAGGCGACATGAGATTCAGGTGCAAACCCGACTTCAGACTACACTTGCCAACT
  TACATCAGAAAAGCAGTGAAATTGAAATTTTGGCTGTGGACCTACCCAAGGAAACAATCTTACAGTTTCT
  ATCATTAGAGTGGGATGCTGATGAACAGGCATTTAACACAACTGTGAAGCAGCTGCTGTCACGCCTGCCA
  AAGCAAAGATACCTCAAACTCGTCTGCGATGAAATTTATAACATCAAAGTTGAAAAGAAGGTGTCAGTGC
  TGTTCCTGTACAGCTACAGAGATGACTACTACAGAATCCTATTTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_013719
• Insert Size: 4947 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013719.3, NP_038747.2
• RefSeq Size: 5220 bp
• RefSeq ORF: 4947 bp
• Locus ID: 27103
• UniProt ID: Q9QZ05
• Cytogenetics: 2 E5
• Gene Summary: Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (eIF-2-alpha/EIF2S1) on 'Ser-52' in response to low amino acid availability (PubMed:10504407, PubMed:10655230, PubMed:12176355, PubMed:12215525, PubMed:15213227, PubMed:16054071, PubMed:16176978, PubMed:16121183, PubMed:15774759, PubMed:16601681, PubMed:26102367). Plays a role as an activator of the integrated stress response (ISR) required for adapatation to amino acid starvation. Converts phosphorylated eIF-2-alpha/EIF2S1 either to a competitive inhibitor of the translation initiation factor eIF-2B, leading to a global protein synthesis repression, and thus to a reduced overall utilization of amino acids, or to a translational initiation activation of specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion (PubMed:10655230, PubMed:11106749, PubMed:12176355, PubMed:15213227, PubMed:16176978, PubMed:26102367). Binds uncharged tRNAs (By similarity). Involved in cell cycle arrest by promoting cyclin D1 mRNA translation repression after the unfolded protein response pathway (UPR) activation or cell cycle inhibitor CDKN1A/p21 mRNA translation activation in response to amino acid deprivation (PubMed:16176978, PubMed:26102367). Plays a role in the consolidation of synaptic plasticity, learning as well as formation of long-term memory (PubMed:16121183). Plays a role in neurite outgrowth inhibition (PubMed:23447528). Plays a role in feeding behavior to maintain amino acid homeostasis; contributes to the innate aversion toward diets of imbalanced amino acid composition (PubMed:16054071, PubMed:15774759). Plays a proapoptotic role in response to glucose deprivation (PubMed:20660158). Promotes global cellular protein synthesis repression in response to UV irradiation independently of the stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK) and p38 MAPK signaling pathways (PubMed:12176355).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.