Zcchc11 (NM_175472) Mouse Untagged Clone
$1,548.00
8 Weeks*
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
|---|---|
| Target Symbol | Zcchc11 |
| Synonyms | 6030404K05Rik; 9230115F04Rik; mKIAA0191; PPAPD3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites | SgfI-NotI | |
| ACCN | NM_175472 |
| Insert Size | 4935 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_175472.3, NP_780681.2 |
| RefSeq Size | 6054 bp |
| RefSeq ORF | 4935 bp |
| Locus ID | 230594 |
| UniProt ID | B2RX14 |
| Cytogenetics | 4 C7 |
| Summary | Uridylyltransferase that mediates the terminal uridylation of mRNAs with short (less than 25 nucleotides) poly(A) tails, hence facilitating global mRNA decay (PubMed:28792939). Essential for both oocyte maturation and fertility. Through 3' terminal uridylation of mRNA, sculpts, with TUT7, the maternal transcriptome by eliminating transcripts during oocyte growth (PubMed:28792939). Involved in microRNA (miRNA)-induced gene silencing through uridylation of deadenylated miRNA targets. Also functions as an integral regulator of microRNA biogenesiS using 3 different uridylation mechanisms (By similarity). Acts as a suppressor of miRNA biogenesis by mediating the terminal uridylation of some miRNA precursors, including that of let-7 (pre-let-7), miR107, miR-143 and miR-200c. Uridylated miRNAs are not processed by Dicer and undergo degradation. Degradation of pre-let-7 contributes to the maintenance of embryonic stem (ES) cell pluripotency (By similarity). Also catalyzes the 3' uridylation of miR-26A, a miRNA that targets IL6 transcript. This abrogates the silencing of IL6 transcript, hence promoting cytokine expression (PubMed:19703396). In the absence of LIN28A, TUT7 and TUT4 monouridylate group II pre-miRNAs, which includes most of pre-let7 members, that shapes an optimal 3' end overhang for efficient processing (PubMed:28671666). Add oligo-U tails to truncated pre-miRNAS with a 5' overhang which may promote rapid degradation of non-functional pre-miRNA species (By similarity). May also suppress Toll-like receptor-induced NF-kappa-B activation via binding to T2BP (By similarity). Does not play a role in replication-dependent histone mRNA degradation (By similarity). Due to functional redundancy between TUT4 and TUT7, the identification of the specific role of each of these proteins is difficult (PubMed:28671666, PubMed:28792939, PubMed:22898984). TUT4 and TUT7 restrict retrotransposition of long interspersed element-1 (LINE-1) in cooperation with MOV10 counteracting the RNA chaperonne activity of L1RE1. TUT7 uridylates LINE-1 mRNAs in the cytoplasm which inhibits initiation of reverse transcription once in the nucleus, whereas uridylation by TUT4 destabilizes mRNAs in cytoplasmic ribonucleoprotein granules (By similarity).UniProtKB/Swiss-Prot Function |
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Resources
"NM_175472" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MG218919 | Zcchc11 (tGFP-tagged) - Mouse zinc finger CCHC domain containing 11 (Zcchc11), (10ug) | 10 ug |
$789.00
$1,607.00
|
|
| MR218919 | Zcchc11 (Myc-DDK-tagged) - Mouse zinc finger, CCHC domain containing 11 (Zcchc11) | 10 ug |
$589.00
$1,407.00
|
|
| MR218919L1 | Lenti ORF clone of Zcchc11 (Myc-DDK-tagged) - Mouse zinc finger, CCHC domain containing 11 (Zcchc11) | 10 ug |
$1,707.00
|
|
| MR218919L2 | Lenti ORF clone of Zcchc11 (mGFP-tagged) - Mouse zinc finger, CCHC domain containing 11 (Zcchc11) | 10 ug |
$1,707.00
|
|
| MR218919L3 | Lenti ORF clone of Zcchc11 (Myc-DDK-tagged) - Mouse zinc finger, CCHC domain containing 11 (Zcchc11) | 10 ug |
$1,707.00
|
|
| MR218919L4 | Lenti ORF clone of Zcchc11 (mGFP-tagged) - Mouse zinc finger, CCHC domain containing 11 (Zcchc11) | 10 ug |
$1,707.00
|
|
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
