Kif14 (NM_001081258) Mouse Untagged Clone

CAT#: MC224772

Kif14 (untagged) - Mouse kinesin family member 14 (Kif14), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Kif14
• Synonyms: D1Ertd367e; E130203M01
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224772 representing NM_001081258
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAGTACACACTTCGCATAGCAGACACAACATCGGAAGCCTGGAGGTTTCTTCTTCACAGAAGATTT
  CAGCATCCAGTGGCCTCGTCCACAGCAGCCGGCTGGAACTGCACTTGAAGGCAGATATGTCAGAGTGTGA
  GAATCATGATCCATTCGTGAATGCTGGAAGTAAAACCATCGACATAAATAGCACTTATGTTATCTCTGCC
  TGTAAAAAAACAAGAGAGACTCCTGTTACCTCTGACCCCCGGAGACTGAGCCTCCAGAGAAGGGCTACTT
  GTGGGGACAGAGAGTCGTCTTTACTTGGAAGTGAGTTGGGAAACAGAAGAACAGCAGACACAAGTCTTAG
  GTTACAACGGCGGCACGGTAGAGCGGATTATGTGGGAAAGTGGGAGACATTGAATCCTGTGGGAGGTAAC
  CCAGGAAGTGACTCTGCCTCCCAGGCATCGAGGACAGAAGCAAAGGGTGTAAATAATGATACCCGTGTCC
  TGTCGTCTGTCGTCTCCGTGAAAGACTCCAACGACACCGGGTTGACGAGATGCAAAGACCCAGGTCCTCC
  CGTTGGTGCCTCTAATGAAAAGGTGACAGTTAAGGACACAAACAGTAGAGCGCCTGTGGGTTCCCAGCGT
  CAGACTGAAGCTATGAGATCAGGACACTTAGTGGTGCAACTGACCGAGAGCAAGTCTGATACCCCAGTGT
  CAGGAGGAAGAAACTCACACCGTGGGAATGCTGGCAAGGACACTGCTAAACAAGTCGGTACATTTGGAAG
  CTCAGATACAAGAACCCCAGTGAAATGTGTTTTAGAACACAGATGGACACCAAGGCATGACCTGCCTCCA
  CCGAAAAGCCCAGCTTTATCCACACTGAAAAACAGGATTGCAAGCCCTCGAGTTAAACCGAGGCCCAAAA
  GTTCTCTTTTTGCAAATAAAAGGGAAAGCTCACGAGAAAGCACACTCCCTCCAGAAGAAAACAGTCTAGT
  TCAGAAGACCTTTACAGAGCCAGACTCCTTAAAAGTAGAGAACAGTCAGGTGACCGTGGCGGTGCGCGTG
  AGGCCTTTCAGCAAAAGAGAGAAGACTGAGAAAGCATCCCAGGTTGTCTTCACCAATGGGGAAGAGATAA
  CTGTGGAGCATCCCGATATGAAACAAGTTTACTCTTTTATTTACGATGTTTCCTTTTGGTCTTTTGATGA
  ATGTCACCCTGGCTATGCCAGCCAGACAACTGTATATGAGACACTAGCTGCACCACTCCTGGACAGAGCT
  TTTGAAGGCTATAACACCTGTCTCTTTGCTTATGGCCAGACTGGCTCTGGAAAGTCCTATACGATGATGG
  GGCTTAATGAAGAACCAGGAATAATTCCTAGATTCTGTGAAGATCTCTTTGCTCAAATAGCCAAAAAACA
  AACCTCAGAGGTCAGCTACCATCTTGAAATGAGCTTCTTTGAAGTTTATAATGAAAAAATTCATGATCTT
  CTGGTTTGTAAAGGTGAAAATGGGCAGAGGAAACAACCACTGCGCGCGAGGGAGCACCCTGTTTCTGGAC
  CGTATGTTGAAGGCCTGTCAATGAATGTTGTGAGTTCTTACTCTGATATTCAGAGCTGGCTGGAACTGGG
  AAATAAACAGAGAGCCACGGCGGCCACCGGCATGAACGATAAAAGCTCCCGGTCTCATTCTGTGTTCACC
  CTGGTGATGACACAGACCAAGACAGAGGTGGTGGAGGGAGAGGAACATGACCACAGGATCACGAGCCGCA
  TCAACCTTGTGGACCTGGCCGGCAGTGAGCGCTGCTCCACCGCCCACTCGAGTGGGCAGCGACTGAAGGA
  AGGTGTGAGCATTAACAAGTCCTTGCTGACTTTGGGGAAGGTCATATCTGCACTCTCCGAGCAAGCAAAC
  GGAAAGAGGGTTTTTATTCCATATCGGGAATCCACTCTTACATGGCTATTAAAAGAAAGCCTGGGTGGAA
  ATTCAAAAACAGCCATGATTGCTACCGTCAGTCCCGCTGCCAGCAACATAGAGGAAACGCTGAGCACACT
  CAGATACGCCACCCAAGCCCGCCTGATAGTCAATATCGCCAAAGTCAACGAGGACATGAATGCAAAGTTG
  ATCAGAGAGTTGAAAGCAGAAATTGAAAAGTTAAAAGCTGCCCAGAGAAGCAATCGGAACATTGACCCTG
  AACGGTACCGACTCTGTCGGCAAGAGATAACGTCCTTAAGGATGAAGCTGCATCAGCAGGAGAGAGACAT
  GGCAGAGATTCAGAGAGTATGGAAGGAAAAGTTTGAACAAGCTGAAAAAAGAAAACTTCAAGAGACAAAG
  GAGTTACAGAAAGCAGGAGTTACATTTCAAATGGACAACCACTTGCCAAACCTTGTCAATCTCAATGAAG
  ACCCACAGCTGTCGGAGATGCTGCTCTACATGGTAAAGGAAGGAGTGACCACAGTTGGAAAGCACACACC
  AAGCTCAAGCCATGACATCCAGTTGTCTGGAGTGCTGATTGCCGATGATCACTGTACTATCAGAAATTTT
  GGAGGAACAGTGAGTATTGTCCCAGCTGGAGAAGCAAAGACATATGTAAACGGGACGCACATCTCGGAGC
  CCACAGTGTTACATCATGGTGATCGGGTGGTTCTTGGTGGAGATCATTATTTTAGATTTAATCATCCAGT
  TGAAGTCCAGAAAGGAAAAAAACTGTCAAGTAGAAATAATCTCACAACAAGCGAAGGCCCAAAAGATTTT
  GAGTTTGCCAAAAATGAGTTACTTACAGCACAGAGGTCTCGGCTTGAGGCAGAAATTAAAGATGCGCAGC
  TGAAGGCGAAGGAAGAAATGATGCAAGGAATTCAGATCGCAAAGGAGATGGCCCAGCAAGAACTTTCTTC
  CCAAAAAGCTGTGTATGAGCGCAAAATCCAGGCCCTAGAGGCGGAGCTGAGGGAAGAGTCTCAAAGGAAG
  AGATTGGAGGAATTAAATAACCAAAAAGCTAGTCACAAGATTGAGGAGTTGGAAAGGGCGAAGCAGCATC
  TTGAACAGGAAGTGTACGTGAACAAAAGGCGACTGGAGATGGAGACCCTGGCCACAAAGCAGGCCTTAGA
  AGACCATAGAATCCGACATGCAAGGATCTTAGAAGCTTTAGAAATTGAAAAGCAAAAGATCGCTGAAGAA
  GTACAAATGCTTCAGGAGAATCGGGGAAACAGGGATAAAACTTTCACCATTCAGCCAAACTGGAACTCCA
  TGAAACTCTCCACGATGATTCAGGAAGCCAATGCCATCAGTGACAAATTTAAAAAGTGCTATATTTTTGG
  CAGACATGATGCATCAGACAAAGGCCGTTCTGATACCTCTGTTCGAGTTCGTAACCTCCAGCTTGGGATC
  TCAACTTTCTGGAGCCTGGAAAAGTTTGAATCTAAACTTGCAGCAATGAAAGAGCTTTATGAGAGTAACG
  GTGGTGACAGGGATGAGGATGTCTTCTGTGACCCTGCAGATGAGTGGGAGCCCGACATTACAAGCACACC
  AGTTTCTTCCCTTTCTAGAAGGAGGAGCAGAAGTTTGATGAAGAACAGGAGAGTCTCTGGCTGTCTGCAT
  GACATCCATCCGATTCAGAGCATGCAGTCTTCACACTCATCTGGATTAATGGAAAAACCGAGCACCATTT
  ACTCAAATTCATCAGAGTCCTTTCTTCCCGGGATTTGCAAAGAATTGATTGGCTCATCAATAGATTTCCT
  CGGACAGAGTTTCGATGAAGAGAAAACTATAGCAGATAGCCTGATAAATAATCTCCTTAGGCTTCATAAT
  GGGGTGATTGCCATCTCCAAAGCCCATGAAGAGCAGGATGAAGAAAGTCAAGATAACCTGTTCTCTGACC
  GGGCGGCACAGGCTCTTACCATCCAGGTTGCTTGTGCCTTTGAGCAGCTTGTGGTTTTGTTCAAACACTG
  GCTGGGTGATTTTCTACCTTGTACTGGCTCAGCGAGGCTTGAAGACGAATTGAGACAAGATATTAAAAAA
  CTCGGAGGCTATTTGCAATTGTTTTTGCAGGGATGCTGTTCGGATATTTCATCGATGGTTAAAGAGGCTC
  AAAACAAAGTCATGAAGATTATACAGCAGGCTGTTCAGTGTGTGGGTCAGCTAGCTGTTCTGAAAGGGAG
  CAAGCTCTGTGTTCTGGAAAACAGCAGCAAAGTCTCCAGTACCCAGGAGTTCATGGCCGCTCTCCAGGAT
  GGTGTAACCTCTGGGATGAAGAGTCTCTTAGACTCTGGGCTGGAGACCGCGCAAGACCTCAGGCAGGACC
  TCTCCAGGCAGAGTGCACGAGAGGAGGTCACTAAGCAGATGAAAGCCAGTACTGTGGAGTGGGTCGGGTC
  TCTGGAAAATGCTGTCGCTGAATGGAGGACGAAAAGCTTCAGAACTCAAGCACAAGAAGGTTCTAGACAA
  CAAGTTTCCAAGCTGTTAAGCCTTGCGTCAGAATTCTTGAAGCTGAAGTCGTGCTTGCAACAAACCGTTG
  AGATGATTGTATCTGCGCTGAGAGGGTGCCCCAGTGACTTGCACTGTCTCAGAAGCTGCACCGAGACTAT
  CTGCAGCCTGGCTCGCAAGCTTCACAGTGACTTCAGCGCACACTCTGCCTCTGCTGGCAGCTGCGGAAAT
  GAACTGCCTCGTGCTGACTGTGAGGAACTGGAGTCTCTAGCGAAGTCACTCCTCCTATGTTTTGAATGTG
  GAGAAAGCCCTGGTTTGTCGAAACCCTGGGAGTCTTGTTCTTCGAATAGCAAGGAAGAGCAGTGCAAGTC
  AGACAGGGCTGACTGCGGGAAAAGCGGGCCAAGACGCGCCTGTGAACCACACGGAGACGCAACCCCAGCA
  GTGTCCTCTGGGGACTGCACCCCGAATAGGATTCAGTGGGTATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001081258
• Insert Size: 4875 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081258.1, NP_001074727.1
• RefSeq Size: 8770 bp
• RefSeq ORF: 4875 bp
• Locus ID: 381293
• Cytogenetics: 1 59.8 cM
• Gene Summary: Microtubule motor protein that binds to microtubules with high affinity through each tubulin heterodimer and has an ATPase activity (PubMed:24949858). Plays a role in many processes like cell division, cytokinesis and also in cell proliferation and apoptosis (By similarity). During cytokinesis, targets to central spindle and midbody through its interaction with PRC1 and CIT respectively (By similarity). Regulates cell growth through regulation of cell cycle progression and cytokinesis. During cell cycle progression acts through SCF-dependent proteasomal ubiquitin-dependent protein catabolic process which controls CDKN1B degradation, resulting in positive regulation of cyclins, including CCNE1, CCND1 and CCNB1 (By similarity). During late neurogenesis, regulates the cerebellar and cerebral cortex development and olfactory bulb development through regulation of apoptosis, cell proliferation and cell division (PubMed:23308235, PubMed:24931760). Also is required for chromosome congression and alignment during mitotic cell cycle process (By similarity). Regulates cell spreading, focal adhesion dynamics, and cell migration through its interaction with RADIL resulting in regulation of RAP1A-mediated inside-out integrin activation by tethering RADIL on microtubules (By similarity).[UniProtKB/Swiss-Prot Function]