Shprh (NM_172937) Mouse Untagged Clone

CAT#: MC224760

Shprh (untagged) - Mouse SNF2 histone linker PHD RING helicase (Shprh), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Shprh
• Synonyms: 2610103K11Rik; AA450458; AU024614; BC006883; D230017O13Rik; E130018M05
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224760 representing NM_172937
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCAGCCGAAGGAAACGTGCTCCCCCGATGAAGGTCGATGAAGAAAGGCAACAGCAGCTTCACTGGA
  ACATGCATGAGGACCTGAGGAGTGAGCCGCTCACCATGACGGTCGGGGAACAGGCCTGTTCAGATGCAGA
  CTCTTCTTCTGATTGCATCATCATCGATGAAGGCCCTCCTGAGAGTGCACTTCACAGAGATAAGAAGAGG
  CGTTCAGAGACTGTGAGCGTCTTAGAGGCCACCGAGGAAGAGACCCGTTTGTCTGTGACTTTGAATGTTA
  CAGTTTCTCCCTATCGTGTGGATAATTCTTGGAAAGCATTTCTGGGAGATTTTGCTCTTCAGCTTCTCCC
  CAAAGAGAGTTTAGTTGAACATTTTTCTGAAAGGACTTTTACATTGAGTCCTTCAGAGTCAAGCAGTCAG
  TTCCTGATCTATGTTCATTCAGAATGTAAAAATGTAGAGAAACAAGAAAATGTTCTTGAAGGATCAGCCG
  GTGTTTGTAGCAAGGGAATCCGAGTGGAATCCTCCTTCAGTAGTGACATGTTACAGGATTTGGCCTGGCT
  ACAGAAGAGAAGAGGAATAAAACTCTACCAGAGACCAGACGGCACTCACACAATCAAGGTTGGAATTTAC
  ATTTTGGAAGCTGGCCTAACAAGACTAGACTTCATGAGTGATGCAGGTTCCAGAATGAAAAAGTTCAATC
  AGCTCATGAAGAGAGTGATGGAAAAGTTACATAATTTCATTATTCCAGATGTGCTGGAAGAAGAGGAGGA
  GGGTTCAGAGAGCGAGCCCGAGGGTCAGGATATTGATGAGCTCTATCACTTTGTGAAGCAAACACACCAG
  CAAGAAACACGGTCAGTCCAAGTGGACGTCCAGCACCCTGCGTTGATTCCGGTCCTGAGACCTTACCAGA
  GAGAGGCAGTCAACTGGATGCTGCAGCAAGAGCAGTTCAGAAGCGCTCCTCCTGCTGATAACTCCCTGCA
  CTTCTTATGGCGAGAAATTGTTACACCTGATGGCTTGAAACTCTACTATAATCCATATACAGGCTGCATC
  ATTCGAGATTTCCCACATGCGGGTCCACAGTTGCTTGGTGGAATCTTAGCAGATGAGATGGGTCTTGGAA
  AGACAGTGGAGGTTCTGGCTCTGATTCTGACACATACCCGACAAGACGTCAAGCAGGACGCTCTCACCCT
  TCCTGAGGGAAAAGTGGTAAATTATTTCATCCCAACTCACTGTCCTAGAGAAAAAGTGAAAAATAGAGAA
  ATCCAGGATACAGAATATGAACCAAAAGAGAAAGTTCACTGCCCTCCTACACGTGTGATGATCCTGACAG
  CTGTGAAAGAAATGAATGGGAAGAAAGGAGTTTCTATTCTTTCTATCTATAAGTATGTCAGCTCCATATT
  CAGATATGATGTTCAGCGGAACCGGGGTCTTCTGAAGCGGATGTTGAAATGTTTAATCTTTGAAGGTCTT
  GTGAAACAGATCAAAGGCCATGGTTTCTCTGGGACTTTTACACTGGGGAAGAATTACAAAGAAGATGTAT
  TTGATAAAACAAAAAAGCAGGCAGTAGGGAGTCCAAGGAAAATTGAGAAGGAATTGAGGAAATCAGTGAA
  CAAGGATGCAGATTCTGAGTACCTGCCATCAAACACTTCTGATGACGATGAGCCTTACTATTATTACTGT
  AAGGCGGGAAAAAGCCGGAGTAAGTTGAAGAAGCCTGCTTTACTAACTAAAAAGGGAAAGGGTCAGTCTG
  TTCATCTGGATTCCCAAGGTGATGCTCCAGCTGCTGGAGTCTGTGCAAGTACTGATGTTCATGTGTCAGA
  AAATACATGTGTCTCTGAAGACAAGCAAACCCAAGAAGCAAAAGACTGTGCTGAGTCGCCAAACCCTGCT
  GCCGAGGAGTTGGCACAGTCTAACACTTCAAGTCCTTGTGAAACCTCTGATTACCGCTTTGAGTGTATAT
  GTGGTGAATTTGATCAAATTGGCCACAAGCCAAGAGTTCAATGTCTGAAGTGTCACCTGTGGCAGCATGC
  AAAGTGTGTGAATTATGAGGAGAAAAACCTGAAGGTTAAGCCTTTTTACTGCCCCCACTGTCTTGTTGCG
  ATGGAGCCAGTATCAACAAGAGCAACTCTGATCATCTCTCCGAGCTCCATTTGCCACCAGTGGGTGGATG
  AAATCAATCGGCACGTGAGGTCATCATCTCTCCGAGTTCTGGTATATCAAGGAGTGAAGAAACACGGCTT
  CTTACAGCCTCATTTTTTGGCAGAACAAGATATAGTTATCATTACCTATGATGTTCTTCGTTCAGAACTA
  AACTATGTCAATATCCCACATAGCAATAGTGAAGATGGGCGTCGCCTGAGGAACCAGAAGCGCTATATGG
  CCATTCCCAGCCCCTTGGTAGCGGTGGAATGGTGGAGGATCTGCCTTGATGAAGCTCAGATGGTCGAGTG
  TCCTACAGTAAAAGCCGCGGAAATGGCCCAGCGTTTGAGCGGGATTAATCGGTGGTGCATCAGTGGCACT
  CCAGTTCAGAGAGGATTGGAAGATCTTTTTGGGCTGGTGGTCTTTCTTGGAATTGAACCTTATTGTGTCA
  AACACTGGTGGATTCGGCTTCTCTATCATCCATACTGCAAGAAAAATCCTCAGCACCTGTACAGTTTTAT
  TGCCAAGATAATGTGGAGGTCTGCAAAGAAAGATGTCATTGACCAAATCCAGATTCCACCTCAGACTGAA
  GAAATGCACTGGCTCCATTTTTCCCCGGTGGAGAGGCATTTCTATCACCGCCAGCACGAGGTGTGCTGCC
  AGGATGCCATAGTGAAGCTCAGGAAGATCTCTGACTGGGCCCTGAAGCTCAGCAGCTTGGACAGGAGAAC
  TGTCTCCTCCATCCTCTACCCACTGCTGAGGCTCCGGCAGGCCTGCTGCCATCCACAGGCTGTTCGTGGG
  GAATTCTTGCCACTCCAGAAGAGCACCATGACAATGGAAGAGTTGCTGACATCTCTGCAGAAGAAATGTG
  GAACGGAATGTGAAGAGGCCCACCGACAACTAGTTTGTGCCCTTAACGGCTTAGCAGGCATTCACATCAT
  TAAAGGTGAATATGCCTTGGCAGCGGAGCTCTACAGAGAAGTATTACGTTCATCTGAGGAGCACAAAGGA
  AAACTCAAAACTGATTCCCTTCAAAGACTGCATGCCACACATAACTTAATGGAGCTGTTGGGAGCCAAAC
  ACCCAGGAATACCTCCCACCTTGAGAGATGGCAGACTTGAAGAAGAGGCCAAGCAGCTGCGAGAGCACTA
  TATGAGTAAGTGTAACACAGAAGTTGCCGAAGCCCAGCAAGCTTTGCAGCCTGTGCAGCAGTCCATTAGG
  GAGCTCCAAAGGAAGATTCATTCTAATTCTCCTTGGTGGCTGAATGTAATCCATAGAGCAATGGAATTTT
  CTGTTGATGAGGAACTTGTTCAGCGGGTGCGAAATGAAATAAGCAGTAACTACAAGCAACAAACTGACAA
  GCTGTCTATGTCAGAGAAGTTCCGTGACTGCAGAGGTCTTCAGTTCTTACTTACAACACAAATGGAAGAG
  CTCCATAAGTTCCAGAAGCTGGTGAGGGAGGCTGTGAAGAAGCTGGAGAAGCCTCCATCCCGGGAGGTGA
  TTGAGTCTGCAACAGTTTGCCACCTGCGGCCAGCTAGACTCCCTCTCAACTGCTGCGTCTTTTGTAAAGC
  TGATGAATTGTTCACAGAGTATGAATCAAAGCTATTTTTTAACACAGTCAAAGGCCAGACTGCAATCTTT
  GAAGAAATGATAGAAGATGAAGAAGGACTTGTAGATGATAGAGTACCTACCACTACCAGAGGTCTGTGGG
  CCGTAAGTGAGACGGAGCGATCTATGAAAGCAATATTATCATTTGCAAGATCACATAGGTTTGATGTTGA
  ATATGTAGATGAAGGAAGTGTTTCAATGGATCTCTTTGAGGCATGGAAGAAGGAATATAAGTTACTTCAT
  GAATACTGGATGACTCTGAGAAATCGTGTATCTGCTGTTGATGAACTTGCAATGGCTACAGAGCGACTAA
  GAGTGCGCCATCCCAAAGAGCCAAAGCCAAACCCACCCGTCCATCACATCATTGAACCACACGAGGTTGA
  ACAGAATCGTATAAAACTGGTGAATGATAAAGCTGTTGCTACATCACAGCTTCAGAAGAAACTTGGACAG
  CTTCTTTACCTAACGAATTTGGAGAAGTCTCAAGATAAAACATCAGGAGGTATTAATCCAGAGCCTTGTC
  CAATCTGTGCTCGACAGCTGGGAAAACAGTGGGCAGTGCTGACCTGCGGACACTGCTTCTGTAATGAATG
  CACTTCGATTATAATTGAACAGTACAGTGTGGGGTCTCATCGAAGTTCCATCAAGTGTGCTATCTGCCGC
  CAGACCACATCACACAAAGAAGTCTCCTATGTCTTCACCTCAGAGAAGGCAAACCAAGAGGATGATATCC
  CTGTGAAGGGTAGCCATTCTACAAAAGTGGAAGCTGTGGTCAGAACTCTGATGAAAATACAACTTAGAGA
  TCCAGGGGCCAAAGCGCTCGTTTTCTCAACGTGGCAAGATGTATTAGATATTATTTCAAAAGCTCTCACG
  GACAACAACATGGAATTTACACAAATCAGTCGCATTAAGACATTCCAGGAGAACCTCTCAGCGTTTAAAT
  ACGATCCCCACATCAATATTCTGCTGCTGCCCCTGCACACAGGCTCTAATGGATTAACTATCATTGAAGC
  CACCCATGTCCTCCTGGTGGAGCCCATACTGAACCCTGCCCATGAGCTGCAGGCCATTGGGAGAGTGCAC
  CGAATTGGACAGACCAAGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_172937
• Insert Size: 4851 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172937.3, NP_766525.3
• RefSeq Size: 7385 bp
• RefSeq ORF: 4851 bp
• Locus ID: 268281
• UniProt ID: Q7TPQ3
• Cytogenetics: 10 A1
• Gene Summary: E3 ubiquitin-protein ligase involved in DNA repair. Upon genotoxic stress, accepts ubiquitin from the UBE2N-UBE2V2 E2 complex and transfers it to 'Lys-164' of PCNA which had been monoubiquitinated by UBE2A/B-RAD18, promoting the formation of non-canonical poly-ubiquitin chains linked through 'Lys-63'.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks two exons and its transcription extends past a splice site that is used in variant 1, resulting in an immediate translation termination and a distinct 3' UTR compared to variant 1. It encodes isoform b which is truncated at the C-terminus, compared to isoform a.