Thoc2 (NM_001033422) Mouse Untagged Clone

CAT#: MC224729

Thoc2 (untagged) - Mouse THO complex 2 (Thoc2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Thoc2
• Synonyms: 6330441O12Rik; D130005M13Rik; Gm1139; Gm1793; Tho2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224729 representing NM_001033422
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCGCGGCTGTGGTGGTTCCCGCAGAGTGGATAAAGAACTGGGAGAAATCAGGGAGAGGCGAAT
  TTTTACATTTATGCCGGATTCTCAGTGAAAATAAAAGCCATGACAGTTCGACCTACAGAGATTTTCAACA
  AGCTCTGTATGAGTTGTCATACCATGTCATTAAAGGGAATTTAAAGCACGAACAGGCATCTAGTGTTCTT
  AATGACATTAGCGAATTCCGTGAGGATATGCCTTCTATCCTTGCTGACGTGTTCTGCATATTAGATATTG
  AAACAAATTGTTTAGAAGAAAAAAGCAAGAGAGACTATTTTACACAGTTGGTATTAGCATGTTTGTATTT
  AGTTTCAGACACAGTTTTGAAGGAACGATTGGATCCAGAAACTTTGGAATCATTAGGGCTTATCAAACAG
  TCACAGCAGTTCAACCAAAAGTCAGTTAAAATCAAGACAAAACTCTTTTATAAGCAGCAAAAATTCAATT
  TATTAAGAGAAGAAAATGAAGGTTATGCCAAGCTGATTGCTGAACTGGGACAAGATTTATCTGGAAATAT
  TACTAGTGATTTAATATTAGAAAATATCAAATCTTTAATCGGATGCTTTAATCTGGATCCTAATAGAGTT
  TTGGATGTCATTTTAGAAGTGTTTGAATGCAGGCCAGAACATGATGACTTCTTTATATCTTTATTAGAAT
  CTTACATGAGTATGTGTGAGCCACAAACACTGTGTCATATTCTTGGATTCAAATTCAAGTTTTACCAGGA
  ACCAAGTGGTGAGACGCCATCGTCTTTATACAGAGTTGCAGCAGTCCTCCTGCAATTTAATCTCATTGAT
  CTAGATGATCTTTATGTGCATCTCCTTCCTGCTGATAATTGTATTATGGATGAATACAAACGAGAAATTG
  TGGAAGCTAAGCAAATTGTAAGAAAACTCACAATGGTTGTGCTGTCTTCTGAAAAACTTGATGAACGAGA
  CAAAGAAAAGGACAAAGATGATGAGAAAGTTGAGAAGCCACCGGACAACCAAAAGCTGGGTTTGTTGGAA
  GCTTTGTTAAAAGTTGGTGATTGGCAGCATGCACAGAACATCATGGATCAGATGCCTCCATACTATGCAG
  CTTCACATAAACTAATAGCTCTTGCTATTTGCAAGCTCATTCACATAACCGTTGAGCCTCTCTACCGAAG
  AGTTGGTGTTCCTAAAGGTGCTAAGGGCTCACCGGTTAGCGCTTTGCAAAACAAGAGAGCACCGAAGCAA
  GTAGAGAGCTTTGAAGATTTGAGGAGGGATGTATTCAATATGTTCTGTTACCTTGGTCCTCACCTCTCAC
  ATGATCCCATTTTATTTGCAAAAGTCGTGCGCATAGGCAAGTCATTTATGAAGGAGTTTCAGTCTGATGG
  AAGCAAACAGGAAGATAAAGAGAAAACGGAAGTTATCCTTAGCTGTTTGCTTAGCATTACTGACCAGGTA
  TTACTTCCATCTCTTTCTTTGATGGACTGCAATGCTTGCATGTCTGAGGAGCTGTGGGGAATGTTTAAGA
  CATTTCCCTATCAGCATAGATATCGACTATATGGTCAGTGGAAGAATGAAACTTATAATGGTCACCCACT
  TTTAGTAAAAGTTAAAGCTCAAACAATAGACAGAGCCAAGTATATCATGAAGCGTCTAACCAAGGAAAAT
  GTGAAGCCTTCAGGAAGACAAATTGGCAAGCTGAGCCACAGCAATCCAACAATTTTGTTTGATTATATCT
  TGTCACAAATCCAGAAATACGATAACTTAATAACACCTGTAGTAGATTCATTGAAATACCTCACTTCGTT
  GAATTATGATGTCTTGGCCTATTGTATCATTGAAGCTTTAGCTAATCCAGAAAAGGAAAGAATGAAACAT
  GATGACACAACCATCTCAAGCTGGCTTCAGAGTCTGGCTAGTTTCTGTGGTGCAGTATTCCGTAAATACC
  CGATTGATCTTGCTGGTCTTCTTCAGTATGTAGCCAATCAATTAAAGGCAGGCAAAAGTTTTGACCTACT
  AATATTGAAAGAAGTGGTACAAAAAATGGCAGGAATTGAAATTACAGAGGAGATGACGATGGAGCAGTTA
  GAAGCCATGACTGGTGGGGAGCAGCTAAAGGCTGAGGGTGGTTATTTCGGCCAGATCAGAAACACTAAAA
  AATCTTCTCAGAGATTAAAGGATGCACTACTGGACCATGATCTTGCTCTTCCTCTCTGTTTGCTTATGGC
  ACAACAGAGAAATGGGGTAATTTTTCAGGAGGGTGGAGAGAAACACTTGAAACTTGTGGGGAAACTCTAT
  GACCAGTGTCATGACACCCTAGTACAGTTTGGTGGATTTTTAGCATCAAATCTAAGTACAGAAGATTATA
  TAAAACGAGTGCCTTCAATTGATGTGCTCTGTAATGAGTTTCATACACCACACGATGCAGCATTTTTTCT
  GTCTAGGCCAATGTATGCACACCATATTTCGTCAAAATACGATGAACTTAAAAAATCAGAAAAGGGAAGT
  AAACAGCAACATAAAGTCCATAAATACATTACATCATGTGAGATGGTGATGGCTCCTGTCCATGAAGCAG
  TGGTCTCCTTACATGTTTCCAAAGTCTGGGATGACATCAGTCCTCAGTTCTATGCTACATTTTGGTCATT
  GACAATGTATGACCTTGCAGTTCCACATACCAGCTATGAAAGAGAAGTCAATAAACTTAAAGTCCAGATG
  AAAGCGATTGATGACAATCAGGAAATGCCTCCAAATAAAAAGAAAAAAGAAAAGGAGCGTTGCACTGCCC
  TTCAGGACAAGCTCCTTGAAGAAGAAAAGAAGCAGATGGAACATGTTCAAAGAGTACTTCAGAGACTGAA
  ACTAGAAAAGGACAACTGGCTTTTAGCAAAGTCTACCAAAAATGAGACCATCACAAAATTTTTACAGCTG
  TGTATATTTCCTCGATGCATTTTTTCAGCAATTGATGCTGTTTATTGTGCTCGTTTTGTTGAATTGGTAC
  ATCAACAGAAAACTCCAAATTTTTCCACACTTCTTTGCTATGATCGAGTTTTTTCTGACATAATTTACAC
  GGTTGCAAGCTGCACTGAAAATGAAGCTAGTCGGTATGGGAGATTTCTTTGCTGTATGTTAGAGACGGTG
  ACCAGGTGGCATAGTGATAGAGCCACATATGAAAAGGAATGTGGAAACTATCCAGGATTCCTTACCATAT
  TACGGGCAACTGGATTTGATGGTGGAAATAAAGCTGATCAGTTAGATTATGAAAATTTTCGACACGTTGT
  CCACAAATGGCATTACAAACTAACCAAGGCATCAGTACATTGCCTTGAGACGGGCGAATATACTCACATC
  AGGAATATATTGATTGTACTAACAAAAATACTTCCTTGGTACCCAAAAGTTTTGAATCTGGGTCAGGCTT
  TGGAGAGAAGAGTGAATAAAATATGCCAAGAAGAAAAAGAGAAGAGGCCAGATCTTTATGCATTGGCTAT
  GGGCTACTCGGGGCAGTTGAAAAGTAGAAAGTCACACATGATACCTGAAAATGAATTTCATCATAAGGAT
  CCTCCTCCAAGGAATGCTGTTGCCAGTGTACAAAATGGGCCTGGTGGTGGGACTTCTTCATCATCTATAG
  GAAATGCATCTAAATCAGATGAAAGCGGTGCTGAGGAGACTGATAAATCAAGGGAGAGATCTCAGTGTGG
  TACAAAAGCTGTTAATAAGGCTTCTAGTACCACACCAAAGGGGAATTCAAGCAATGGAAATAGTGGCTCT
  AACAGCAACAAAGCTGTTAAGGAAAATGACAAAGAAAAAGTAAAAGAGAAAGAAAAAGAGAAAAAGGAGA
  AGACTCCAGCTACAACTCCAGAGGCCAGGGCACTTGGTAAAGATAGTAAAGAAAAACCGAAGGAAGAGCG
  GCCAAATAAAGAAGACAAAGCAAGAGAGACAAAGGAAAGAACACCTAAATCTGACAAAGAGAAAGAGAAG
  TTCAAGAAGGAAGAAAAAGCTAAAGATGAGAAATTCAAGACTACCGTCCCCATCGTAGAATCAAAATCGA
  CTCAAGAAAGGGAAAGAGAGAAGGAGCCATCCAGAGAAAGAGATGTAGCAAAGGAAATGAAGTCAAAGGA
  AAATGTTAAAGGAGGAGAAAAAACACCAGTTTCAGGGTCCTTGAAGTCACCTGTTCCCCGATCAGATATC
  TCGGAGCCTGACAGGGAACAAAAACGCCGCAAAATTGATAGTCATCCTTCTCCATCACATTCCTCCACAG
  TAAAGGACAGTCTCATCGATCTCAAGGACTCTTCAGCAAAGCTCTACATTAACCATAACCCTCCACCACT
  GTCCAAGAGTAAGGAGAGAGAAATGGACAAGAAAGATTTGGACAAGTCAAGGGAAAGATCCAGAGAAAGA
  GAGAAAAAAGATGAAAAGGACAGGAAAGAACGGAAAAGGGATCATTCAAACAATGACCGCGAAGTGCCAC
  CGGACATAACAAAGAGGCGGAAAGAGGAGAATGGAACAATGGGGGTTTCAAAACACAAAAGTGAGAGTCC
  ATGTGAGTCTCAATATCCAAATGAGAAAGACAAAGAGAAAAATAAGTCAAAATCTTCAGGCAAAGAAAAA
  AGCAGTAGTGATTCGTTTAAGTCTGAGAAGATGGATAAAATCTCATCTGGTGGCAAAAAGGAATCCAGGC
  ATGATAAAGAAAAGATAGAAAAGAAAGAAAAACGGGATAGTTCAGGAGGAAAGGAAGAGAAGAAACATCA
  TAAGTCCTCTGACAAGCACAGATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001033422
• Insert Size: 4785 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033422.1, NP_001028594.1
• RefSeq Size: 7631 bp
• RefSeq ORF: 4785 bp
• Locus ID: 331401
• UniProt ID: B1AZI6
• Cytogenetics: X A4
• Gene Summary: Required for efficient export of polyadenylated RNA and spliced mRNA. Acts as component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. Plays a role for proper neuronal development.[UniProtKB/Swiss-Prot Function]