Hecw2 (NM_001001883) Mouse Untagged Clone

CAT#: MC224704

Hecw2 (untagged) - Mouse HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (Hecw2), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Hecw2
• Synonyms: A730039N16Rik; D030049F17Rik; Gm971; mKIAA1301; Nedl2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224704 representing NM_001001883
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTAGCTCAGCCCGGGAGCATTTGCTTTTTGTGCGGCGTCGAAATCCACAGATGCGGTACACACTGA
  GCCCAGAGAACCTCCAGAGCCTGGCAGCCCAGAACTCAATGCCCGAGAACATGGCCCTACAGCGGGCCAA
  CAGTGACACGGATCTGGTGACTTCTGAGAGCCGCTCCAGCCTGACTGCCAGCATGTACGAATACACGCTG
  GGGCAAGCCCAGAACCTCATTATCTTCTGGGACATTAAGGAAGAGGTTGACCCCAGCGATTGGATAGGAC
  TCTATCACATAGATGAGAATTCTCCAGCCAACTTCTGGGACTCTAAAAACAGGGGTGTGACTGGAACCCA
  AAAAGGACAAATTGTATGGCGAATTGAGCCTGGGCCCTACTTCATGGAACCGGAGATCAAAATCTGTTTC
  AAATACTACCATGGTATTAGTGGAGCCCTGCGAGCCACAACCCCCTGTATTACCGTGAAGAACCCAGCTG
  TGATGATGGGGGCCGAAGGCATGGAAGGAGGTGCTTCAGGAAGCCTGCACTCTAGGAAACTCGTCAGCTT
  CACATTGTCAGATCTCAGGGCAGTTGGGCTCAAGAAAGGGATGTTCTTCAATCCTGACCCTTATCTTAAG
  ATGTCCATTCAGCCAGGGAAGAAGAGCAGTTTTCCTACCTGCGCCCACCATGGGCAGGAGAGAAGGTCCA
  CTATCATCAGCAACACCACCAATCCGATCTGGCACAGAGAGAAATACTCCTTTTTTGCACTTTTGACTGA
  TGTCTTAGAAATTGAAATCAAAGACAAATTTGCCAAGAGCCGGCCCATCATCAAACGCTTCCTGGGGAAA
  CTAACCATTCCAGTGCAGAGGCTGCTGGAGCGGCAGGCTGGGGACCAAATGCTCAGCTACAACCTTGGCA
  GACGGCTCCCAGCTGACCACGTGAGTGGGTACCTCCAGTTTAAAGTGGAAGTTACATCTTCTGCTCACGA
  AGATGCTTCTCCAGAGGCTGTCGGCACCATCCTCGGAGTCCACACTGTGAATGGAGACCTGGGCAGCCCT
  TCCGATGAGGAGGATATGCCAGGCAGCCATCATGACAGTACTATCTGTGCTAATGGGCCAGTGTCCGAGG
  ACAGTGTTGCTGATGGCACCCCTAAGCATTCTTTCAGGACTAGCTCTACCCTGGAGATCGACACGGAGGA
  CTTGATCTCTACTTCTTCTAGAAATTCACCCCCCAGGGGCCGCCAGGATTCCCTCAATGATTACTTAGAT
  GCTATTGAACACAATGGCCCCGCCAGGCCAGGGGCAGCCTCCTCCTCTGAGCGGTCCATGGGAGCCTCTC
  CTAAACTGAGGAGTAGTTTTCCCACTGACACAAGACTCAATGCGATGCTTCATATTGACTCAGACGAAGA
  AGACCATGAGTTTCAGCAAGACTTGGGCTACCCATCTTCTTTGGAGGAGGAGGGAGGCTTGATCATGTGC
  AGCAGGGCGTCGAGAATTGATGATGGGAGCCTGACATCTCAGACCAAACCAGAGGATGACAACCCTGTGG
  AGAATGAGGATGCCTCTATACATGAAACTGCATCCTTAGAAGAGAGGCTGGAGAATCTTCCAGAGGTTGC
  AGATGGCTCCTTGCCATCAAGTACAGCCCCTGATGAAAATGAAGCCAACCTGGAGCCTCAACCCAGTGCA
  GACCAGGGTAGCACTGAATTGTGTAGCTCTCAAGAAGTAGATCAGCCCACAAGTGGTGCAGATGCAGGAG
  CTTCTGACACATCTGGAGGGAGCCGCAGAGCTGCCAGTGAGACCGAGTCCCTGGACCAGGGATCTGAACC
  TTCCCAGGTGTCCTCTGAGACAGAACCCAGTGACCCTGCAAGGACAGAGAGTGTGAGTGAGGCCAGTACC
  AGACCTGAGGGGGAGAGTGACCCGGAAGGCGCAGACAGCTCATGCAATGAGAGCGTGACTACACAGCTGT
  CCTCCGTGGAGACACGGTGCTCATCTCTAGAGAGTGCGCGATTTCCAGAAACCCCCGCCTTTTCTTCTCA
  GGAGGAAGAAGATGGGGCCTGTGCAGCTGAGCCCACCAGCAGTGGCCCCGCAGAGGGGTCGCAGGAGTCC
  GTCTGCACTCCTAGTTCTTTACCTGCTGTGCAGGTGCCCAGCAGGGAAGAGGAAGGGTCAGCGGCTGAAG
  CTGCTGCTTTATCTGAGCAGGGGGAGCTAGGGGAAGTCTGGCAGAGGAGGGGGAGTCTTGAGGGAGCAGC
  CGCTGCTGCTCCTGCTGCTGCTGCCACTGATAGCCAGCCCCAGGAAGATGGGGATGCTGGGGATGCCCAA
  GGTGCCTGCGAAGGGGCCACTGCCCAGGAGGAGGGCGCCACTGGAGGTTCTCAAACCAATGGCCACCAGC
  CCTTGAGGTCACTACCTTCCGTGCGCCAGGACGTCAGCCGGTACCAGAGGGTGGATGAGGCTCTCCCACC
  AAACTGGGAGGCACGGATTGACAGCCACGGCAGGATCTTCTATGTGGACCATGTAAACAGAACCACAACG
  TGGCAGCGGCCCACAGCTCCCCCTGCCCCACAGGTCCTGCAGCGATCTAACTCCATACAGCAAATGGAGC
  AGCTGAACCGACGTTATCAGAGCATCCGCAGAACCATGACTAATGAGAGGCCTGAGGAAAATACCAGTGC
  TATCGATGGGGCAGGGGAGGAAGCCGACTTTCACCAAGCCAGCGCAGACTTCCGTCGGGAGAACGTCCTG
  CCTCACTCTACCTCCAGATCCAGGCTCACGCTGCTGTTACAGTCACCCCCCGTGAAGTTCCTCATCAGCC
  CAGAGTTCTTCACCGTGCTGCATTCTAACCCTAGTGCCTACCGCATGTTTACAAACAACACGTGTTTGAA
  GCACATGATCACCAAAGTCCGGCGGGACACCCACCACTTTGAGCGCTATCAGCATAACAGGGACCTCGTG
  GGATTCCTCAACATGTTTGCTAACAAACAGCTGGAACTACCAAGGGGCTGGGAGATGAAACATGATCATC
  AGGGCAAGGCGTTTTTTGTCGACCACAACTCCCGGACCACCACGTTCATTGATCCTCGGCTCCCACTTCA
  GAGCAGTAGGCCCACAAGTGCATTGGTTCATCGACAACACCTGACGAGGCAACGCAGCCACAGTGCGGGT
  GAGGTAGGAGAAGACTCTCGGCACGCAGGACCACCAGTTCTTCCCAGGCCATCCAGTACATTCAATACGG
  TCAGTAGGCCGCAGTACCAGGACATGGTTCCAGTGGCCTACAATGACAAAATTGTTGCGTTTTTGCGTCA
  ACCCAACATCCTTGAAATTCTGCAAGAGCGTCAACCTGATCTTGCCAGAAACCACTCACTCAGGGAGAAG
  ATCCAATTTATCCGAACGGAAGGGACCCCTGGATTGGTGCGGCTCTCAAGCGATGCAGACCTTGTCATGT
  TACTGAGCTTATTCGAGGAAGAGATAATGTCATATGTGCCTCCTCATGCCTTACTCCACCCCAGCTACTG
  TCAGTCCCCGCGTGGCTCCCCCGTATCATCTCCCCAGAACTCACCAGGTACTCAGCGTGCCAATGCCCGG
  GCTCCAGCCCCTTACAAGAGAGATTTTGAAGCCAAACTAAGGAACTTTTACAGGAAGTTAGAAACTAAAG
  GATACGGACAAGGCCCAGGAAAATTAAAGCTAATTATCCGAAGAGATCACTTGCTGGAAGATGCTTTCAA
  TCAGATCATGGGCTACTCCAGAAAGGACCTGCAGAGGAATAAACTGTACGTCACCTTTGTCGGGGAGGAA
  GGGTTGGATTACAGTGGGCCATCCAGAGAGTTCTTCTTCCTGGTATCCCGAGAGCTCTTTAACCCCTATT
  ACGGCTTATTTGAATATTCAGCCAATGACACATATACAGTGCAAATAAGTCCCATGTCTGCCTTTGTAGA
  CAATCATCATGAATGGTTCCGGTTCAGCGGGAGGATCCTCGGCCTTGCGCTCATACACCAGTACTTGCTG
  GACGCCTTCTTCACGCGACCCTTCTATAAGGCTCTTCTCAGAATCCTGTGTGACCTGAGTGATCTGGAAT
  ACCTTGATGAGGAGTTCCACCAGAGCCTTCAGTGGATGAAAGACAATGACATCCATGACATCCTGGACCT
  CACGTTCACTGTGAATGAAGAAGTTTTTGGGCAGATTACTGAACGAGAGTTAAAACCAGGGGGAGCCAAT
  ATTCCAGTCACAGAGAAGAACAAGAAGGAGTACATCGAGAGAATGGTGAAGTGGAGGATCGAGAGGGGTG
  TGGTGCAGCAGACAGAGAGCCTAGTTCGTGGTTTCTACGAGGTAGTAGATGCCAGGCTAGTGTCTGTCTT
  TGATGCGAGAGAGCTGGAACTGGTCATTGCCGGCACAGCTGAGATAGATCTCAATGACTGGAGAAACAAC
  ACTGAATACAGAGGCGGGTACCATGACAATCACATTGTAATTCGGTGGTTCTGGGCTGCAGTGGAAAGAT
  TCAACAATGAACAACGACTAAGGTTGTTACAGTTTGTTACAGGCACATCCAGCATTCCCTATGAAGGATT
  CGCTTCTCTCCGAGGGAGTAATGGTCCAAGAAGATTCTGTGTGGAAAAATGGGGAAAAATCACCGCTCTT
  CCCAGAGCCCACACTTGTTTTAACCGCCTGGATCTGCCTCCATACCCTTCCTTTTCCATGCTTTATGAAA
  AACTCTTGACAGCAGTTGAAGAGACCAGTACCTTTGGACTTGAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001001883
• Insert Size: 4737 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001001883.3, NP_001001883.1
• RefSeq Size: 11179 bp
• RefSeq ORF: 4737 bp
• Locus ID: 329152
• UniProt ID: Q6I6G8
• Cytogenetics: 1 C1.1
• Gene Summary: E3 ubiquitin-protein ligase that mediates ubiquitination of TP73. Acts to stabilize TP73 and enhance activation of transcription by TP73. Involved in the regulation of mitotic metaphase/anaphase transition.[UniProtKB/Swiss-Prot Function]