Med1 (NM_013634) Mouse Untagged Clone

CAT#: MC224683

Med1 (untagged) - Mouse mediator complex subunit 1 (Med1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Med1
• Synonyms: AI480703; CRSP210; DRIP205; l11Jus15; PBP; Pparbp; TRAP220
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224683 representing NM_013634
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTCCCTCCTGGAACGGCTCCATGCAAAATTTAACCAGAACAGACCTTGGAGTGAAACCATTAAGC
  TTGTGCGTCAAGTAATGGAGAAGAGGGTCGTAATGAGTTCTGGAGGGCATCAGCATTTGGTCAGCTGTTT
  GGAGACATTGCAGAAGGCTCTCAAAGTAACATCTTTGCCAGCAATGACTGATCGTTTGGAATCTATAGCC
  AGACAGAATGGACTGGGCTCTCACCTCAGTGCCAGTGGCACTGAGTGTTACATCACGTCAGATATGTTCT
  ATGTGGAAGTGCAGTTAGATCCTGCAGGACAGCTTTGTGATGTCAAAGTGGCTCACCATGGGGAGAATCC
  TGTGAGCTGTCCAGAGCTTGTACAGCAGTTAAGGGAAAAGAATTTTGAGGAATTTTCCAAGCATCTTAAG
  GGTCTTGTTAATCTGTATAATCTCCCAGGGGACAACAAACTGAAGACTAAAATGTATCTGGCTCTCCAAT
  CCTTAGAACAGGACCTTTCTAAAATGGCTATTATGTACTGGAAGGCAACCAACGCCGCTCCCTTGGATAA
  GATTCTTCATGGAAGTGTTGGTTATCTCACCCCGCGGAGTGGGGGTCATTTAATGAATATGAAATACTAT
  GCCTCTCCATCTGACCTGCTGGATGATAAGACTGCCTCTCCTATCATTTTGCATGAAAAGAATGTTCCTC
  GGTCTTTGGGAATGAATGCCTCAGTGACAATTGAAGGAACCTCTGCTATGTACAAACTCCCAATTGCCCC
  ATTAATTATGGGGTCACACCCAGCTGACAACAAATGGACCCCTTCTTTCTCCGCAGTCACTAGTGCCAAC
  AGTGTTGATCTTCCTGCGTGTTTCTTCTTGAAATTTCCCCAGCCAATTCCAGTATCTAAAGCATTTGTTC
  AGAAACTGCAAAATTGCACAGGAATCCCGTTGTTTGAGACTCCGCCCACTTACCTGCCCCTGTATGAACT
  CATCACTCAGTTTGAGCTGTCAAAGGATCCTGACCCTTTACCTTTGAATCACAACATGCGATTTTACGCT
  GCTCTTCCAGGTCAGCAGCACTGCTATTTTCTCAATAAAGATGCTCCTCTTCCTGATGGTCAGAGCCTGC
  AGGGAACACTGGTCAGCAAAATCACCTTCCAGCACCCTGGCCGAGTTCCTCTTATCTTGAATATGATCAG
  ACACCAAGTGGCCTATAACACTCTAATTGGAAGCTGTGTCAAAAGAACTATTTTAAAAGAAGATTCTCCT
  GGGCTCCTCCAGTTTGAAGTGTGTCCTCTCTCAGAATCTCGCTTCAGTGTATCTTTTCAGCACCCTGTGA
  ATGACTCCCTTGTGTGTGTGGTGATGGATGTGCAAGACTCAACACATGTGAGCTGTAAACTCTACAAGGG
  GCTGTCAGATGCACTAATCTGTACAGACGACTTCATTGCCAAAGTTGTTCAAAGATGTATGTCCATTCCT
  GTGACGATGAGGGCTATTCGGAGGAAGGCTGAAACCATACAGGCTGACACCCCAGCACTGTCTCTCATTG
  CAGAGACAGTTGAAGACATGGTGAAAAAGAACCTGCCCCCGGCTAGCAGCCCAGGGTATGGCATGACCAC
  AGGCAACAACCCAATGAGTGGTACCACTACACCAACCAACACCTTTCCGGGGGGTCCCATTACCACCTTG
  TTTAATATGAGCATGAGCATCAAAGATCGGCATGAGTCGGTGGGCCATGGGGAGGACTTCAGCAAGGTGT
  CTCAGAACCCAATTCTTACCAGTTTGTTGCAAATCACAGGGAACGGGGGGTCTACCATTGGCTCGAGTCC
  GACCCCTCCTCATCACACGCCGCCACCTGTCTCTTCGATGGCCGGCAACACCAAGAACCACCCGATGCTC
  ATGAACCTTCTTAAAGATAACCCTGCCCAGGATTTCTCAACCCTTTATGGAAGCAGCCCTTTAGAAAGGC
  AGAACTCCTCTTCCGGATCACCCCGGATGGAAATGTGCTCGGGGAGCAACAAGGCCAAGAAGAAGAAGTC
  GTCAAGAGTCCCACCTGACAAACCCAAGCACCAGACTGAAGACGATTTCCAGAGGGAGCTCTTTTCCATG
  GATGTCGACTCACAGAACCCTATGTTTGACGTCAGCATGACCGCTGACGCGCTGGATACACCTCATATCA
  CCCCAGCTCCAAGCCAGTGTAGCACTCCCCCAGCAACGTACCCACAGCCAGTGTCTCACCCCCAGCCCAG
  TATTCAGAGGATGGTCCGACTGTCCAGTTCAGACAGCATTGGCCCAGATGTAACTGATATTCTTTCAGAT
  ATTGCCGAAGAAGCTTCAAAGCTTCCCAGCACGAGTGATGACTGCCCACCAATTGGCACCCCTGTTCGAG
  ATTCCTCAAGTTCTGGGCATTCTCAGAGTGCCCTCTTTGATTCTGATGTCTTTCAAACTAATAATAATGA
  AAATCCATACACTGATCCAGCTGACCTTATTGCAGATGCTGCTGGAAGCCCCAATAGTGATTCTCCTACC
  AATCATTTTTTCCCTGATGGAGTAGATTTCAATCCTGATTTGTTGAACAGCCAAAGCCAAAGTGGTTTTG
  GAGAGGAGTATTTTGATGAAAGTAGTCAGAGTGGGGATAATGATGATTTCAAAGGATTTGCATCTCAGGC
  ATTAAATACATTGGGGATGCCAATGCTTGGAGGTGACAATGGGGAGCCAAAATTTAAGGGCAGCAGCCAG
  GCTGACACGGTGGACTTCAGTATTATATCAGTAGCCGGTAAGGCTTTGGGTGCTGCAGATCTGATGGAGC
  ACCACAGTGGGAGTCAGAGTCCTTTACTGACCACTGGAGAATTAGGGAAAGAAAAAACTCAAAAGAGGGT
  GAAGGAAGGCAACGGCACAGGTGCTAGCAGTGGATCAGGTCCAGGGTCAGACAGCAAGCCAGGCAAGCGC
  AGCCGCACTCCCTCCAATGATGGGAAGAGCAAGGATAAGCCTCCAAAGCGGAAGAAGGCAGACACTGAGG
  GGAAGTCCCCATCTCACAGTTCTTCTAATAGACCTTTCACCCCACCTACCAGCACGGGTGGGTCCAAATC
  CCCAGGCAGTTCAGGACGATCTCAGACGCCCCCAGGTGTTGCCACCCCGCCCATTCCCAAGATTACCATT
  CAGATTCCTAAAGGGACAGTGATGGTGGGCAAGCCCTCCTCTCACAGTCAGTACACTAGCAGTGGTTCTG
  TGTCTTCCTCTGGCAGCAAAAGCCACCATAGTCATTCTTCCTCCTCCTCTTCCTTAGCTTCTGCTTCCAC
  CTCAGGCAAGGTGAAAAGCAGTAAATCTGAAGGCTCATCAAGTTCCAAGCTCAGTGGCAGTATGTATGCT
  AGCCAAGGGTCTTCTGGATCCAGCCAGTCCAAAAATTCATCTCAGACTGGGGGGAAGCCAGGCTCCTCTC
  CCATTACCAAACATGGACTGAGCAGTGGGTCCAGCAGTACCAAGATGAAACCTCAAGGCAAGCCATCCTC
  CCTTATGAACCCTTCTATAAGTAAGCCAAACATATCCCCTTCCCATTCAAGGCCTCCCGGAGGCTCAGAT
  AAGCTTGCCTCTCCAATGAAGCCTGTTCCTGGAACCCCCCCATCCTCTAAAGCCAAGTCCCCTATCAGTT
  CAGGTTCCAGTGGTTCTCATGTGTCAGGAACTAGTTCAAGCTCTGGTATGAAGTCATCTTCAGGGTCAGC
  ATCCTCAGGCTCAGTGTCTCAAAAAACCCCTCCAGCATCTAATTCTTGTACACCATCTTCCTCTTCGTTT
  TCCTCAAGTGGTTCTTCCATGTCATCCTCTCAGAATCAACATGGCAGTTCCAAAGGGAAATCTCCCAGTA
  GGAATAAGAAGCCTTCCTTGACAGCTGTCATAGATAAATTGAAGCATGGGGTGGTTACCAGTGGGCCTGG
  GGGTGAGGATCCAATAGACAGTCAGATGGGCGCAAGCACAAATTCTTCTAACCATCCCATGTCCTCCAAA
  CATAACACGTCAGGAGGGGAGTTCCAGAGCAAACGTGAGAAAAGTGATAAAGACAAATCCAAGGTCTCTG
  CTTCTGGGGGGTCAGTGGATTCCTCTAAGAAGACTTCAGAGTCAAAAAATGTGGGGAGCACGGGGGTGGC
  AAAAATCATTATCAGCAAGCACGACGGAGGCTCCCCGAGCATCAAAGCCAAAGTGACGCTACAGAAACCT
  GGAGAAAGTGGTGGAGATGGGCTCAGGCCACAGATAGCCTCATCAAAGAACTATGGCTCTCCACTTATCA
  GTGGTTCCACTCCAAAGCACGAACGGGGTTCTCCCAGCCACAGTAAGTCGCCAGCATATACACCACAGAA
  TGTGGACAGTGAAAGTGAGTCAGGCTCCTCCATAGCAGAGAGATCCTACCAGAACAGTCCCAGCTCAGAG
  GATGGTATCCGACCACTTCCAGAGTACAGCACTGAGAAGCATAAGAAGCACAAAAAGGAAAAGAAGAAAG
  TCAGAGACAAAGACAGAGACAAGAAGAAGTCTCACAGCATGAAGCCAGAGAACTGGTCGAAATCCCCCAT
  TTCTTCAGATCCGACGGCGTCTGTGACAAATAACCCTATCTTATCTGCAGACAGGCCTTCTAGGCTCAGC
  CCTGACTTCATGATTGGGGAGGAAGATGATGATCTCATGGATGTGGCCCTGATTGGCAATTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_013634
• Insert Size: 4683 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013634.2, NP_038662.2
• RefSeq Size: 6383 bp
• RefSeq ORF: 4683 bp
• Locus ID: 19014
• UniProt ID: Q925J9
• Cytogenetics: 11 61.75 cM
• Gene Summary: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Essential for embryogenesis, including development of the central nervous system, heart, liver and placenta and for erythropoiesis. Also required for normal transcriptional control of thyroid-stimulating hormone beta (TSHB) in the pituitary. Acts as a coactivator for GATA1-mediated transcriptional activation during erythroid differentiation of K562 erythroleukemia cells (By similarity).[UniProtKB/Swiss-Prot Function]