Pcf11 (NM_029078) Mouse Untagged Clone

CAT#: MC224677

Pcf11 (untagged) - Mouse cleavage and polyadenylation factor subunit homolog (S. cerevisiae) (Pcf11), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pcf11
• Synonyms: 2500001H09Rik; 5730417B17Rik; C77803
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224677 representing NM_029078
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAGAGCAGACGCCGGCGGAGGCCGGGGCTGCGGGGGCCCGGGAGGACGCCTGTCGGGATTATCAGT
  CGTCGCTGGAAGACCTGACCTTCAATAGCAAACCGCACATCAATATGCTGACCATTCTAGCCGAGGAGAA
  CCTGCCCTTCGCCAAGGAGATCGTCTCTCTCATCGAGGCCCAAACCGCCAAGGCTCCTTCCTCAGAGAAG
  CTTCCAGTTATGTACCTTATGGATTCTATCGTGAAAAATGTTGGAAGAGAATATCTCACTGCCTTTACTA
  AAAATCTAGTTGCAACATTTATTTGTGTGTTTGAAAAGGTGGATGAAAATACTAGAAAAAGTTTATTTAA
  ATTACGTTCCACATGGGATGAAATATTCCCTTTGAAGAAACTTTATGCCTTGGATGTCAGAGTCAATTCA
  TTAGATCCTGCTTGGCCTATTAAACCTCTGCCTCCTAATGTGAATACATCTAGCATCCATGTGAATCCCA
  AATTTTTAAATAAATCGCCTGATGAGCCTTCAACACCTGGCACAGTGGTCAGTTCCCCCAGTATCTCCAC
  TCCTCCCATTGTACCTGATATACAAAAGAATCTTACCCAAGAACAACTAATAAGGCAACAGTTACTGGCA
  AAACAAAAACAGTTGTTAGAACTTCAGCAGAAAAAGCTGGAGCTTGAGTTAGAACAAGCTAAGGCACAGC
  TGGCAGTATCTCTGAGTGTTCAGCAGGAAACAGCCAACTTGGGTCCTGGATCAGTGCCGTCTAAATTACA
  TGTTCCACAAATTCCCACAATGGCTGTTAAAACTCCCCATCAGGTCCCTGTACAACCTGACAAAAGCCGA
  GCAGGTCCATCCTTACAAATGCAAGACTTGAAAGGAACTAACCGGGATCCCCGTCTTAACAGGATGAGCC
  AACATTCTTCCCATGGCAAAGAGCAGAGTCATAGGAAAGAATTTGTAATGAACACAATAAACCAGTCTGA
  TATTAAAACAAGTAAGAATGTACCCTCTGAAAAGCTAAATTCATCCAAACAAGAAAAAAGTAAATCAGGT
  GAAAGAATAACCAAGAAAGAACTTGACCAGTTAGATTCTAAATCCAAATCTAAGTCTAAATCACCATCAC
  CTTTGAAAAACAAATTATCCCACACAAAAGACTTGAAAAATCAAGACTCTGAAAGTATGAGGTTGTCTGA
  TATGAGTAAGAGAGATCCACGATTAAAGAAGCATCTTCAGGATAAAGCTGAGGGCAAAGATGAAGATGTA
  AAAGAAAAGAGAAAAACTGCAGAAAAGAAGGAGAAAGATGAGCACATGAAATCATCTGAACACAGGGTGA
  TTGGAAGTAGAAGTAAAATCATAAATGGCATTGTCCAAAAACAAGACATGGTTACAGAGGAGTTGGAAAA
  ACAGGGGACAAAACCAGGGAGATCGAGTACTAGAAAGAGATCAAGATCAAGGTCACCTAAGTCTCGGTCA
  CCAATTATACATTCCCCAAAGAGAAGAGATAGACGGTCACCCAAACGAAGGCAAAGAAGTATGTCTCCCA
  ATTTGGCACCCAAAGCTGGAAAGATGCGTCAGTCGGGACTAAAACAGTCACATATGGAAGAATTCCCACC
  ACCTTCCAGGGAAGAAAGAAATATTAAGAGAAGTGCTAAGCAGGATGTTAGAGATCCCAGACGACTGAAA
  AAGATGGATGAGGACCGGCCTCAAGAAACTGCAGGTCAACATTCTATGAAGTCAGGTGGTGACCCAAAGG
  AGAATATAGAGAATTGGCAAAGCTCTAAGTCTGCCAAAAGATGGAAATCTGGCTGGGAAGAAAATAAAAG
  CTTGCAGCAGGGTGATGAACATAGTAAACCTCCTCATCTAAGGCATAGGGAGAGCTGGTCAAGCACTAAA
  GGGATCTTGTCACCTCGAGCCCCCAAGCAGCAGCACCGACTAAGTGTAGATGCCAATCTTCAAATTCCTA
  AAGAGTTAACTCTTGCAAGCAAAAGAGAATTGCTTCAAAAGACGAGCGAACGTTTAGCATCTGGTGAAAT
  TACACAGGATGAGTTCCTTGTTGTTGTGCATCAAATTCGACAGCTATTTCAGTATCAAGAAGGTGTACGA
  GAGGAGCAGAGATCACCATTCAATGATCGTTTTCCACTTAAGCGACCTAGATATGAAGATTCAGATAAAC
  CATTTGTAGATGGCCCAGCATCAAGATTTGCTGGCCTTGATACAAATCAGCGACTTACAGCTCTAGCTGA
  AGACCGACCATTATTTGATGGACCTGGTAGGCCATCTGTGACGAGAGATGGCCCAGCCAAGATGATTTTT
  GAAGGACCTAATAAATTAAGCCCTAGAATTGATGGACCTCCTACACCAGGTTCTCTTCGGTTTGATGGGT
  CACCAGGACAAATGGGGGGAGGAGGCCCTATGAGATTTGAAGGACCACAAGGTCAGTTAGGAGGTGGGTG
  TCCTTTGAGATTTGAAGGTCCTCCAGGACCAGTAGGAACACCTCTGCGGTTTGAAGGGCCAATTGGTCAA
  GGAGGAGGAGGTGGTTTTCGATTTGAAGGTTCACCTAGTTTGAGGTTTGAGGGATCTACAGGTGGTTTAC
  GATTTGAAGGACCAGGGGGTCAGCCCGTGGGTGGTCTCAGGTTTGAAGGACATCGTGGTCAACCTGTGGG
  TGGTCTCAGGTTTGAAGGACCTCATGGACAGCCTGTGGGCAGTCTTAGATTTGATAATCCTCGAGGTCAG
  CCTGTAGGAGGACTTAGATTTGAAGGGGGTCATGGTCCATCAGGGGCTGCAATTAGGTTTGATGGGCCTC
  ATGGTCAGCCAGGTGGTGGTGGTGGTATCAGATTTGAGGGCCCTTTGCTACAGCAAGGAGTTGGAATGAG
  GTTTGAGGGTCCCCATGGTCAGTCTGTAGCTGGTCTGAGATTTGAAGGACATAATCAACTAGGTGGGAAC
  CTTAGGTTTGAGGGACCACATGGTCAACCAGGGGTTGGGATCAGGTTTGAAGGACCTATAGTTCAACAAG
  GAGGTGGAATGAGGTTTGAAGGTCCTGTACCAGGAGGTGGCCTGAGAATTGAAGGGCCTCTGGGTCAAGG
  TGGTCCTAGATTTGAAGGTTGTCACTCTTTAAGGTTTGATGGACAGCCAGGTCAACCATCACTTTTGCCA
  AGATTTGATGGATTACATGGCCAGCCAGGTCCTAGATTTGAAAGAACTGGTCAGCCAGGTCCACAGAGGT
  TTGATGGACCACCTGGACAGCAGGTTCAACCAAGATTTGATGGTGTACCTCAAAGATTTGATGGGCCACA
  ACACCAGCAAGCATCAAGATTTGATATTCCTCTTGGTCTACAAGGAACTCGATTTGACAATCATCCTTCA
  CAAAGGATTGAATCTTTCAATCATTCTGGCCCATATAATGATCCACCTGGCAATACTTTTAATGTTCCAT
  CTCAAGGATTACAGTTCCAAAGACACGAACAAATATTTGATACACCTCAAGGACCAAATTTTAATGGACC
  ACATGGCCCTGGAAATCAGAATTTCCCAAATCCCATTAACAGAGCTTCTGGACACTATTTTGATGAAAAG
  AATCTTCAGAGTTCTCAGTTTGGAAACTTTGGCAATTTGCCTACACCAATATCAGTAGGAAATATTCAGG
  CATCTCAACAGGTTCTTACTGGTGTTGCTCAGCCAGTAGCGTTTGGCCAAGGACAACAGTTCTTACCAGT
  TCATCCACAGAATCCTGGAGCTTTTATTCAAAATCCTTCAGGCGGTCTTCCCAAGGCATATCCTGATAAT
  CATCTCAGTCAGGTGGATGTAAACGAATTGTTTTCAAAACTGCTAAAAACAGGAATTCTCAAATTGTCAC
  AGCCTGATTCAGCTACAGCACAGGTAACCGAGGCTGTTGCTCAGCCTCCCCCTGAGGAGGACGAGGATCA
  GAATGAAGATCAGGATGTTCCAGATCTTACCAATTTCACCATTGAAGAATTGAAGCAACGTTATGACAGT
  GTTATAAACCGGCTGTACACTGGGATTCAATGTTACTCGTGTGGAATGAGGTTCACGACATCCCAGACAG
  ATGTTTATGCAGATCACTTGGATTGGCATTATCGGCAAAACAGAACCGAGAAAGATGTTAGCAGAAAAGT
  CACTCATAGACGTTGGTACTACAGTCTAACAGATTGGATAGAATTTGAAGAAATAGCTGATCTGGAAGAA
  CGTGCAAAAAGCCAGTTTTTTGAAAAGGTTCATGAAGAGGTTGTCCTTAAAACTCAGGAGGCTGCTAAAG
  AAAAGGAGTTCCAAAGTGTACCTGCTGGACCAGCTGGAGCAGTTGAGAGTTGTGAAATCTGTCAAGAACA
  ATTTGAACAGTACTGGGATGAAGAGGAGGAAGAATGGCACTTAAAAAATGCTATTAGAGTAGACGGAAAG
  ATTTACCATCCATCATGTTATGAAGATTATCAAAATACATCTTCATTTGATTGCACACCATCTCCCAGCA
  AGACACCAGTTGAAAACCCTTTGAACATTATGTTGAACATTGTCAAAAACGAATTGCAAGAACCCTGTGA
  AAGTCCCAAAGTTAAGGAAGAACAAATTGATGCCCCACCAGCTTGTTCAGAAGAAAGTGTAGCAACACCC
  ACTGAAATTAAAACAGAAAGTGATACAGTTGAGTCAGTTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_029078
• Insert Size: 4662 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029078.3, NP_083354.3
• RefSeq Size: 5804 bp
• RefSeq ORF: 4662 bp
• Locus ID: 74737
• Cytogenetics: 7 E1