Arhgef12 (NM_027144) Mouse Untagged Clone

CAT#: MC224664

Arhgef12 (untagged) - Mouse Rho guanine nucleotide exchange factor (GEF) 12 (Arhgef12), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Arhgef12
• Synonyms: 2310014B11Rik; AU019857; Larg; mKIAA0382
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224664 representing NM_027144
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGGCACACAGTCTACTATCACGGACAGGTTTCCCCTCAAAAAACCTATAAGGCATGGAAGTATTT
  TGAACCGAGAGTCACCAACAGATAAGAAACAGAAAGTTGAACGCAGTTCATCGCATGATTTTGACCCCAC
  AGATAGCTCCTCCAAGAAGACAAAGTCTAGTTCAGAGGAGAGTAGATCCGAGATCTATGGTCTTGTTCAG
  CGCTGTGTGATCATCCAGAAAGACGACAATGGCTTCGGGCTGACAGTCAGTGGAGACAATCCGGTCTTCG
  TACAATCTGTCAAAGAAGATGGAGCAGCCATGCGTGCTGGAGTACAAACAGGTGATCGAATCATCAAGGT
  GAATGGTACTCTGGTGACTCATTCAAATCATCTGGAGGTGGTGAAACTAATCAGATCTGGTTCCTATGTA
  GCTCTCACTGTCCAGGGACGCCCACCTGGGTCGCCCCAGATCCCACTTGCTGATTCTGAAGTAGAGCCAT
  CAGTCACTGGACATATGTCTCCCATTATGACATCCCCTCATTCCCCTGGAGCAGCCGGGAATATGGAGAG
  AATCACCAGTCCTGTGCTCGTGGGGGAGGAAAACAATGTGGTCCATAACCAGAAAGTAGAGATTCTGAGA
  AAGATGTTACAGAAGGAACAGGAACGGCTGCAGTTGCTGCAGGAAGATTACAACCGAACGGCTACCCAAA
  GATTGCTCAAGGAGATCCAGGAGGCCAAGAAGCACATCCCGCAGCTGCAGGAGCAGCTGTCCAAAGCCAC
  GGGCTCTGCTCAGGACGGAGCTGTAATTGCACCCTCCAGGCCATTAGGTGATGCCCTAACACTCAGTGAG
  GCAGAAGCAGATCCTGGAGATGGCCTGTGCAGGACTGACTGGAGCAGTGGAGATGCTTCTCGGCCCAGTA
  GTGACAGTGCTGATAGTCCCAAGAGTAGCCTGAGAGAAAGGAGTTACCTGGAGGAAGCCCCAGAGAGAAG
  TGAAGGAGTTCAGGACGCTCAGGAGCCGCAGTCGCTGGTCGGCAGTCCCTCCACCCGGGGCGCCCCTCAC
  ATCATTGGAGCAGAGGATGATGATTTTGGTACTGAGCATGAGCAGATCAATGGACAGTGCAGCTGTTTCC
  AGAGCATTGAATTGCTGAAGTCTCGCCCTGCTCACTTGGCTGTTTTCTTACACCATGTAGTCTCACAGTT
  TGACCCTGCAACATTGCTCTGTTATCTCTATTCAGACCTGTATAAGCAGACAAACTCCAAGGAGACTCGG
  CGTGTCTTCCTGGAGTTTCATCAGTTCTTCTTGGACCGGTCTGCACACCTGAAGGTTCCAGTTCCTGAGG
  AAATCTCCGTGGATCTAGAAAAGAGAAGACCTGAGCTTATTCCTGAGGATCTGCATCGCCTCTACATTCA
  GACAATGCAGGAGAGAGTCCACCCTGAAGTTCAGAGGCACTTGGAGGATTTCCGACAGAAACGCAGCATG
  GGGCTGACCTTGGCTGAGAGTGAGCTGACTAAACTTGATGCAGAGAGAGACAAGGACCGGGGAACTCTGG
  AGAAGGAGCGAGCATGTGCGGAACAGATCGTTACCAAAATTGAAGAAGTGTTGATGACTGCTCAGGCTGT
  AGAAGAAGAAAGGAGTTCCACAATGCAGTATGTAATTCTCATGTATATGAAGTATTTGGGAGTAAAAGTG
  AAAGAACCTCGAAACTTGGAGCACAAGCGGGGTCGGATCGGCTTCCTTCCAAAGATCAAGCAAAGCATGA
  AGAAAGATAGAGAGGGCGAAGAAAAAGGGAAGCGAAGAGGATTCCCCAGCATTTTGGGACCCCCAAGAAG
  ACCAAGCCGCCACGACAACAGTGCAATTGGCAGGGCCATGGAGATACAGAAGTCACGCCACCCGAAGCAC
  TTATCCACTCCCTCGTCTGTGAGTCCTGAGCCTCAGGACCCTGCCAAGTTGCGCCAGAGTGGAGTAGCCA
  ATGAAGGAACAGACACTGGATACCTGCCCGCCAGTTCCATGTCCTCTGCCACCTCAGGGACTGCTCTTTC
  CCAAGAGGGAGGGAGAGAGAATGATACAGGAACAAAACAAGTGGGAGAAGCATCGGCGCCTGGAGACTGC
  TTGGACAGCACACCTCGAGTCCCCACTACTGTTTTCGACTTCCCTCCTCCTCTATTAGATCAAGTGCAGG
  AGGAGGAGTGTGAAGTAGAGAGGGTGGCAGAACATGGGACCCCAAAACCCTTTCGGAAGTTTGACAGCAT
  CGCCTTTGGAGAAAGTCAGAGTGAGGATGAGCAGTTTGAAAATGACCTGGAGACAGATCCACCCAACTGG
  CAGCAGCTTGTTAGCAGAGAAGTGTTACTGGGACTGAAACCTTCTGAGATCAAAAGACAGGAAGTGATTA
  ATGAATTGTTCTACACTGAGAGAGCTCATGTCCGGACACTGAAGGTTCTTGACCAAGTATTCTATCAGCG
  AGTGTCCAGAGAAGGGATTCTATCACCTTCAGAACTACGCAAGATTTTTTCAAACTTGGAAGATATCCTT
  CAACTTCATGTTGGGTTGAATGAGCAAATGAAAGCTGTCCGAAAAAGGAATGAAACTTCTGTTATTGATC
  ATATTGGGGAAGATCTGCTCATCTGGTTTAGTGGACCAGGAGAGGAGAAGCTGAAACATGCTGCTGCCAC
  ATTCTGCAGTAACCAACCTTTCGCCCTGGAAATGATCAAGTCTCGCCAGAAAAAGGATTCTCGATTCCAT
  ACTTTTGTGCAAGACGCTGAGAGTAACCCCCTGTGCCGTCGTCTTCAGCTGAAGGACATCATTCCCACCC
  AAATGCAGAGACTCACTAAGTACCCGCTTCTTTTGGATAACATTGCCAAGTACACAGAATGGCCTCCAGA
  AAGGGAAAAGGTGAAGAAGGCTGCAGACCATTGTCGTCAGATCTTAAATTATGTAAATCAGGCTGTCAGG
  GAGGCAGAAAACAAGCAGCGCTTAGAAGACTATCAGCGTCGCCTTGACACCTCCAATCTCAAGTTGTCCG
  AGTACCCAAATGTTGACGAGCTCAGGAATTTGGATTTAACAAAAAGAAAGATGATTCATGAAGGGCCGTT
  GGTCTGGAAGGTGAATAGAGATAAAAGCATCGATCTTTATACACTGCTGCTGGAAGACATCCTTGTGCTG
  CTACAGAAGCAGGATGACAGGCTGGTGCTAAGGTGCCACAGCAAGATTCTGGCATCCACAGCCGACAGCA
  AACACACCTTCAGCCCTGTCATTAAGCTGAGCACGGTGCTGGTTCGGCAGGTGGCGACAGATAACAAAGC
  TTTATTCGTCATCTCCATGTCAGATAATGGAGCCCAAATTTATGAATTGGTAGCACAGACGGTTTCTGAA
  AAGACAGTCTGGCAAGACCTCATCTGTCGAATGGCTGCGTCAGTGAAGGAGCAGTCTACAAAGCCGATTC
  CCCTCCCACAGCCGCCACCTTGCGAAGGAGACAATGATGAAGAAGAACCAGCCAAGTTAAAAGTGGAGCA
  CCATGATCTTTCAGTCGCTGGTCTGCAAAGTCCAGACAGAGTTTTGGGATTAGAATCTCCCTTAATTTCG
  TCAAAGCCTCAGTCTCATTCACTGAATACCCCTGGCAAATCAGCGGCAGAACATCTCTTTGTGACCGCAA
  CACAGTTTGCAAAGGAACAGCATGCAAATGGGGCACTCAAGGAAGGTGATGGCGGGTATCCAGTCACAAT
  CCCAGGTCCACACTTGCCTGTGTCAGAGGAGCGGTGGGCATTGGACGCACTGAGAAATTTGGGCTTGTTG
  AAGCAGTTGCTGGTACAGCAGTTAGGTCTCACCGAGAAGAGTACTCAGGAAGACTGGCAGTCCTTCTCAC
  GATACGGGCCAGCTTCTGAAGAGGTACAGGCAGACAGTGGCATCCGGGACTTGGAAAATGTTAAGGCTTG
  TCATGCCAGGGAAGGACAGATGTCTTTTAAAACTGGAACTGGTGATATTGCAACTTGTGACAGTCCACGG
  ACTTCAACTGAGTCTTGTGCTGCACAGGATTCAGTGATACTGGCATCCCAAGACAGCCAGGCAAGTAACG
  TTTTAGTAATGGACCACATGATCCTGACCCCAGAGATGCCTCCTGCAGAGCCAGAAGGGGGTCTGGATGA
  GAGTGGAGAGCACTTTTTTGATGCCCGTGAAGCACATAGTGATGATAATCCATCAGAAGGTGATGGAGCA
  GTTAAAAAGGAAGAGAAGGATGTTAATTTACGCATCTCAGGAAACTGTTTGATCCTTGATGGCTATGATG
  CAGTTCAGGAGAGCTCCACAGATGAGGAGGTTGCCTCCTCGTTCCCCCTGCAGCCTGTGACGGGCATCCC
  CGCTGTGGACTCCAGCCACCAGCAGCAACATTCCCCTCAGAATGTGCATCCCGAGGGGCCGGTTTCACCA
  TTCACCCCAGAATTCCTGGTCCAGCGGCACTGGCGAGCTATGGAGGATACCTGTTTTGAGATCCAGAGTC
  CCTCATGTACAGATTCACAAAGCCAAATCCTGGAGTACATTCATAAAATAGAGGCTGACCTGGAACACTT
  AAAGAAGGTGGAGGAAAGTTACGCTCTTCTTTGCCAAAGGCTGGCTGGCTCAGCCCTCCCCGACAAGCTC
  TCAGATAAAAGTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_027144
• Insert Size: 4635 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027144.2, NP_081420.2
• RefSeq Size: 10483 bp
• RefSeq ORF: 4635 bp
• Locus ID: 69632
• Cytogenetics: 9 A5.1
• Gene Summary: May play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13). Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase and may act as GTPase-activating protein (GAP) for GNA12 and GNA13 (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.