2810474O19Rik (NM_026054) Mouse Untagged Clone

CAT#: MC224643

2810474O19Rik (untagged) - Mouse RIKEN cDNA 2810474O19 gene (2810474O19Rik), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: 2810474O19Rik
• Synonyms: 6720435I21Rik; AA536804; AU020969; GET; Kiaa1551
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224643 representing NM_026054
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATTGGAATACAAAACAAGAGAATGTTCCCAAACCCCCACCATATTCTAAAACCCAGTCATCTATCT
  TGCAGCATTTTTTAATGACTTCTACAACATCTCAAAGTTCTTTCAACTATTCTCCCCATAACCAAGAAGC
  ATCTCAAACTTCTTTCAACTATTCTCTCCATAACCAAGAAGCATGCATGTATTCTGGGAATTCAAATTCA
  GTTTCACAGCCACTTCTGAGCGGCAGAAATTATATAACTCCTCAGACTCAGATCTCTGTTTCTAATATGC
  CTACCAGAACAATTGTGGCCTCACAGTCTTCAATGGAAAGAGTTGTATCTACAAATGGTAAAGGACCACA
  ACAACCAAACCACAATTTGCAAACAGTGTCTTCAGGAATTATGCAGAATGTCTGGTTGCCTTCCCATACA
  GAAGCAACCATATCTCATAATCCCGACGGAGGGACTAATATGCCTTACATGCATCCACCACAGAATCAGC
  TTGTCACATCAGATACCTATTCTATGCAACTACAAATGGCTCCTTTACATTCTGGAAAAGTACCTATGAC
  ACATCAAGGAAGTCAGGGACTTAATCACTTCATACCAGATCAGTTGGTTGACTGGACACAGTATACATCC
  AACGAACTTTCTTATCCTGAATACAGGCCACCTCCAAAGCAATACTCCTACATACTGCCAGCTACTACAT
  CATTACAAGTTAAAAATAATCAGCTCCCAACTTATACACAGAGCTTACAGTCAAAGCATTCTGTACCTTT
  GTCATCACATCAGTATGCTGCAGAAGCCAGCAAAAGACTCTCTGCCCTTCCTTACAGCTGTAGATATGAA
  AACCAACATGTGCAGAATGCTCAGCCTGTTTCTAAACACTTGCCTATGGAAGTTCCTCAGAGTTCAGAAG
  TGCACTCATCTGAAAAAAAGAAAGATACTTACAGAGGCTTTAAGCAGCAGTGGCAGAACCCTAATGAGAA
  AGTCAGCATTGGACAATTCTCTGAGGTGAAAATAAATATCAAGCAGCCTTACAGTGAATCTGTTAGACCT
  TCTGGGGATGGTGTTCAGGCACTTGTTCAAAATAATCAAGAAAAAAGAAAATATACCTACAATCCTAATA
  CAAATCAAGTAATAGATACAAATGCCACAAAAGAAAAGCTGGTGAGGGATATTAAATCTCTAGTAGAAAT
  CAAAAAGAAGTTTTCAGAACTTGCAAGAAAAATTAAAATTAATAAAAGTCTTTTGATGGCAGCTGGTTGT
  AGTAAAACAGCTAATACTTCTTATACTGAACCAATTCAGCATTCTGAATTTTCAGCAAAAGAAATGTCTG
  CTAAAAATGGCAATGACTGTTCCATGGAATTGCTAGCAACATGTCTTTCTCTTTGGAAAAACCAACCTTC
  AAAAACCACAGAAGAAAATGTTCCAAAACCTTTAGAAGAAAAACAGTGTAACACATCAAGAATCAGTACA
  ACCGTAGTGGGCAGTGCCAATCCCACAAATGAAGTTCATGTTAAGAGTTTGTGTTCTGGTGTTGGAAATT
  CTCAGAAAATGATGAGCTCATCACAAACAGTATTGCCAGTTCTCATACCGAGCTGTGAGTCTTCCGGTGT
  AGCTGTAGGAAAAGGAACAGAGCTTCAGATTGCTGTGGTGTCGCCTTTAGTTCTTTCAGATACTAATACC
  TTACCTGGAAAAGACTCAGTGCCTGAAGTTTTACCTGAAACACTGTATCCAGTTGTTAAGGAAGGTAGTG
  TTTGTAGCTTACAAACCCAACCAACAGAAACTGTTGCTTTACCTTTTGATGTTATAGGAGCTGTAGCAAG
  TAATAACATATCAGCAGAGATTCCTCTGCCTGTTGATAAGGAAAAGCAGCACAAACCAATACAGGGTGAT
  CCAGACATAGCTGATAGCAGCTTAGGAAAACACTCTCCCCTGGGCACTGAAGTTCTGCCTAAGCCTATGG
  ATAGCACCATTGTGAGTGGACCTATGTTACAAATCGAAAGTATCTGTTCTCTTGCAGAAGGGGATGTATC
  TTACAATTCCCAGATAGCTGAGATATTCAACTCTGTACAAACTGAGCCCCAGAAACCTTCACCTAATCAG
  GTAATTGATAGTCAACAAGAACAAGTATATGATACCACTGAAAATAAAGACTTTAGCTTACAGAAAGATA
  AGTGTGTACAGTGTACAGATGTTCCTCATGAGGTCCCTGAGCAGCCAGAGCCTCTGCAGCCTGAAGAGCC
  AGCATCTAGTGAGTATGTGGAAGCAAACAGAGAAGCCACAGAGGAAAGCTGTAGGGAGTACACTGGTAGA
  AAAGAAAGTACAGCTAAGGATGTATGTTTACCAGCTGCTATTCAGCAGGATCCTCACCCTCGGGAAACTG
  ATATGTTCAGTAAATCAGACCACAGTCTTCCTGCAATAAATGAGATTAATGATGAAAGTGAACCTATCTC
  ATACCTACATGACCAGCTGTCAGAGCTTTTAAAAGAGTTCCCTTATGGCATTGAAACTTTTAACAGACAT
  GAAGTGTCTTTGGACCAACAAAAGACACATAAAATCGTAGAAAATCAAACTGGTGGTAAAACTAGTAATG
  TGTCTGGGGATAGCACAGACCAAATAAAAATTACAGTATTAAACTCTGAACAAATCAAAGAACTATTTCC
  TGAAGATGATCAGCCCTGTGACAAGTTGGCGGAACCTGAGAATAAAGAAATTGTTGCGGAAGTAAAGAGC
  CCATGTGACTCACAGATTCCTAGAGAAGAAAGTCATGACCTTGGAATGTTGGATCCAGAGAAAGACAAAA
  TCCATTGCTGTGCCTTGGGTTGGCTCTCAATGGTGTATGAAGGTGTGCCACAGTGTCATTGCAGTTCTAC
  AGAAAAGAAGGAGAAAGACCAGTGTTTGGACATCAACAGCAGCAAACAAGGAGAGCAGCCCTGCAATAGT
  GGAATCACTATTTTTGAAATTAATCCTGTTTCTAATAACTCAAAAACTCCTCTGACCCAAGCAACTGAGG
  AAGGCCATTTTTCTGCAGTGCATGGTGAAAAGACCAAAGCATCTAAAACAAAAGACAACAGGGAGGGACA
  GGAATTAGCCTGTCATTTTTCAGCTAAATGTTACAAAAAAGATAAAAAAGGTAATTTTAAAATAAGGCAT
  GATACCTCACTGAAAATGGAGCAAAAACTAAAAAATATTTCATCTAAATGTGACATACCAAACCCCTCAA
  AATGCAATAAGATAGCAGCCCCTGAAATACTTCACGTAACAACTTCTAACTCTGCTAAAAATATGCCATT
  TTCTAAACAAGCCTCTCAGGAAAGCCTACAGAAGAAACACACGTCCCAAGACTTGGGTCCAGTAAAAGCA
  CCTATAGAACTTTCGTCAAACACAGATCCGTGCAGGAGTAATACCTCTTCAGTACAGTCAGTATCACCAG
  AAAAGAAAAAACTAAAATTCAAAGCAGGTGGCTCCAGGTTGAAATATTTTGAAAAAAGAAAGACGGATCA
  CGTTATTATTCCAGATGTAGAAATAAAAAAGAAGAAATATGAAAAGCAAGAACAGAACAAAAATGCTGGA
  GACACACTCAAATTGTGCAGTATTCTTACAGAGTCAAATGAAAGAGCCAGTGTTCAAGAAAAGACAGTTC
  CAAGTCCTGAGTCCTCAGACCCAAAGGGTAGCTCCTCTAAGAGTACTAGAGTTATAACAGTGCAGGAATA
  TTTACAACGGCAGAAAGACAAACAAATAACTGGGAATAATGCTTCCAGAAACATCTGTGTAGAAACTGTG
  CTATGTGACTCGGGGCACACGAAGACCAGTAAGCATTCTGCAGCAGTAAGCTGGGGAAAGTTAGTTGAGG
  GGCAGAGTATCAGTGCAGAGACTGCAAAAGAACTGGAACATAATTCTAGCAGCCATGGTAAAGACTTCAA
  GATCCATCACTCTGAGGCGTCTAGAACACACAGTGTGTCAAATAATAATAAAGGAAAGTTTGATGGGAAG
  CAGCCTGATAAAATGTTTAAAAATAAGACCAGTATGAATAATGAGTCCAACCAGATGCCTCTCCAGGTAA
  AAGAACAAAGGAAACAGTACTTGAATCGAGTTGCGTTTAAATGCACAGAACGGGAAAGCATATGTCTCAC
  CAAATTGGATAGTGCATCCAAGAAGCTTAGTATAGAGAAAAAGAGTGGGGAATATACATCTAAGACAAAA
  GACACAGACAAACCTAGTATGCTGGAGTTTAAATTATGTCCAGATGTGCTATTGAAGAATACAAGCACTG
  TTGACAAGCAGGATTGTCCTGGTCCTGGTCCTGAGAAAGAGCAAGCACCTGTGCAAGTTTCAGGAATAAA
  AAGTACAAAAGAAGACTGGTTAAAATGCATCCCTACAAGGACAAAGATGCCTGAATCAAGTCAAAGAGAT
  AGTGCTGACTCAAGACTTTCTAAGAGAAGCCTCAGTGCAGATGAATTTGAAATACTACAAAACCCAGTAA
  AAGAATCAAATATCATGTTCCGGACCTACAAAAAGATGTACCTTGAGAAGAGAAGCAGAAGCCTCGGGAG
  TAGTCCAGTTAAATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_026054
• Insert Size: 4566 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_026054.3, NP_080330.1
• RefSeq Size: 6094 bp
• RefSeq ORF: 4566 bp
• Locus ID: 67246
• UniProt ID: Q5DTW7
• Cytogenetics: 6 G3
• Gene Summary: Plays a role in the regulation of imprinted gene expression, regulates repressive epigenetic modifications associated with SETDB1. Required for the recruitment or accumulation of SETDB1 to the endogenous retroviruses (ERVs) and maintenance of repressive chromatin configuration, contributing to a subset of the SETDB1-dependent ERV silencing in embryonic stem cells.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) lacks an exon in the 5' UTR, compared to variant 1. Variants 1, 2, and 3 encode the same protein.