Top2a (NM_011623) Mouse Untagged Clone

CAT#: MC224642

Top2a (untagged) - Mouse topoisomerase (DNA) II alpha (Top2a), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Top2a
• Synonyms: Top-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224642 representing NM_011623
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTTGTCACCGCTGCAGCCTGTAAATGAAAATATGCTAATGAACAAAAAGAAGAATGAAGATGGCA
  AGAAAAGATTGTCTATTGAAAGAATCTATCAGAAAAAAACACAACTGGAACATATACTGCTCCGCCCAGA
  TACCTACATTGGCTCTGTGGAGTTAGTGACCCAGCAAATGTGGGTTTACGATGAAGATGTTGGCATTAAC
  TACAGGGAAGTCACTTTTGTTCCTGGTTTGTATAAAATCTTTGATGAGATTCTAGTTAATGCTGCTGATA
  ACAAACAAAGGGACCCAAAAATGTCTTGTATTAGAGTCACAATTGATCCAGAAAATAATGTAATTAGCAT
  CTGGAATAATGGGAAAGGAATCCCTGTTGTTGAACACAAAGTTGAGAAAATATATGTCCCAGCTCTCATA
  TTTGGACAACTCCTGACCTCCAGTAACTATGACGACGATGAGAAGAAAGTGACAGGTGGTCGAAATGGCT
  ATGGAGCTAAACTGTGTAACATATTCAGCACCAAATTTACTGTGGAAACAGCCAGTAGAGAGTACAAGAA
  AATGTTCAAACAGACGTGGATGGATAACATGGGGAGAGCTGGTGACATGGAACTCAAGCCCTTTAGTGGA
  GAAGATTATACATGTATCACCTTCCAGCCTGACTTATCTAAGTTTAAAATGCAAAGCCTGGACAAAGATA
  TTGTTGCACTGATGGTCAGAAGAGCATATGATATCGCTGGCTCCACTAAAGATGTCAAAGTCTTTCTTAA
  TGGAAATAGTCTGCCAGTGAAAGGATTCCGCAGTTACGTGGATTTGTATTTGAAGGATAAGGTAGATGAA
  ACTGGGAATTCACTGAAGGTGATACATGAACAGGTCAATCCCCGGTGGGAAGTGTGCTTAACAATGAGCG
  AGAGAGGCTTCCAGCAGATTAGCTTCGTCAACAGCATTGCGACTTCTAAGGGCGGCAGACATGTCGATTA
  TGTAGCTGATCAGATTGTGAGCAAACTCGTTGATGTGGTGAAAAAGAAGAACAAGGGCGGGGTTGCAGTG
  AAGGCTCATCAGGTGAAAAATCACATGTGGATTTTTGTAAATGCCTTAATTGAAAACCCAACCTTTGACT
  CTCAAACAAAAGAAAACATGACTTTACAAGCCAAGAGCTTTGGATCAACATGTCAATTAAGTGAAAAGTT
  CATCAAAGCTGCAATTGGTTGTGGTATTGTGGAAAGCATACTAAACTGGGTGAAGTTCAAGGCTCAAATC
  CAACTGAACAAGAAGTGTTCAGCTGTAAAACATACCAAAATCAAGGGAATCCCCAAACTTGATGATGCCA
  ACGATGCAGGGAGCCGAAACTCTACTGAATGCACACTTATCCTGACTGAGGGAGACTCAGCAAAAACTCT
  AGCCGTTTCAGGCCTTGGAGTGGTTGGAAGAGACAAATATGGGGTGTTCCCTCTTAGAGGAAAGATACTC
  AATGTGCGAGAAGCCTCTCATAAACAGATCATGGAAAATGCTGAAATTAACAATATCATCAAGATTGTGG
  GTCTCCAGTACAAGAAAAACTATGAAGATGAAGATTCATTGAAAACTCTTCGTTATGGGAAGATCATGAT
  TATGACAGATCAGGACCAAGATGGTTCCCACATCAAAGGCTTGCTGATCAATTTTATCCATCACAATTGG
  CCATCTCTTCTGCGACATCGTTTTCTAGAAGAATTTATCACTCCCATTGTAAAGGTGTCCAAAAATAAGC
  AAGAAATAGCATTCTATAGTCTTCCCGAGTTTGAAGAATGGAAAAGTTCGACTCCGAATCATAAAAAGTG
  GAAAGTCAAATACTACAAAGGTTTGGGCACCAGCACATCAAAGGAAGCTAAGGAATATTTTGCAGATATG
  AAACGACATCGTATTCAGTTCAAATACTCTGGCCCTGAAGATGATGCTGCGATCAGCCTGGCCTTTAGTA
  AAAAACAAGTTGATGATCGAAAGGAATGGTTAACTAATTTTATGGAAGACAGAAGACAGCGGAAGTTACT
  TGGCCTTCCTGAGGATTATTTGTATGGACAAAGCACTTCGTACCTGACCTATAATGACTTCATAAACAAG
  GAACTCATCCTCTTCTCCAACTCTGATAATGAAAGGTCTATCCCATCCATGGTAGACGGTTTGAAACCAG
  GTCAGAGAAAGGTTTTGTTTACTTGTTTCAAACGGAATGACAAGCGAGAAGTGAAGGTTGCCCAGTTAGC
  TGGGTCAGTGGCAGAAATGTCCTCTTATCACCATGGTGAGATGTCACTGATGATGACCATTATCAATTTG
  GCGCAGAATTTTGTGGGTAGCAATAATTTGAACCTTTTACAGCCCATTGGTCAGTTTGGAACCAGGCTGC
  ATGGTGGCAAGGACTCAGCTAGTCCTAGGTACATCTTTACAATGCTCAGCCCTTTGGCTCGGTTGTTGTT
  TCCTCCAAAAGATGATCACACATTACGGTTTCTATATGATGACAACCAACGTGTTGAGCCTGAATGGTAC
  ATTCCTATAATACCCATGGTGCTGATAAATGGTGCTGAGGGAATTGGTACTGGGTGGTCCTGCAAAATCC
  CCAACTTTGATGTGCGTGAAGTTGTGAATAATATCAGACGGCTTCTGGATGGAGAGGAGCCCCTACCCAT
  GCTCCCAAGTTATAAGAATTTCAAAGGTACTATTGAAGAACTGGCTTCAAATCAGTATGTGATTAATGGA
  GAAGTAGCTATTCTGGATTCTACAACCATTGAAATCTCAGAGCTTCCCATCCGAACGTGGACTCAGACAT
  ATAAAGAACAGGTTCTAGAACCCATGTTGAATGGAACTGAGAAGACACCCTCTCTAATAACAGACTATAG
  GGAATACCATACAGATACCACTGTGAAGTTTGTCATAAAGATGACTGAAGAAAAACTGGCAGAGGCAGAG
  AGAGTCGGCCTACACAAAGTCTTCAAACTCCAGAGTAGTCTCACTTGCAACTCTATGGTGCTTTTTGACC
  ATGTAGGTTGCTTAAAGAAATATGACACTGTGTTGGATATTCTAAGAGACTTCTTTGAGCTCAGGCTTAA
  ATATTATGGATTAAGAAAAGAATGGCTTCTAGGAATGCTTGGTGCAGAATCTTCTAAACTGAATAATCAA
  GCTCGCTTTATATTAGAGAAAATAGATGGCAAAATAGTCATTGAAAATAAACCTAAAAAAGAATTAATTA
  AAGTTCTGATTCAGAGAGGCTACGACTCTGACCCTGTGAAAGCCTGGAAAGAAGCTCAGCAGAAGGTTCC
  AGATGAAGAAGAAAATGAAGAAAGTGACACTGAAACCAGCACCAGTGACTCTGCAGCCGAGGCTGGGCCG
  ACCTTCAACTACCTTCTCGACATGCCCCTGTGGTATCTGACCAAGGAGAAGAAGGATGAGCTGTGCAAAC
  AAAGAAACGAGAAGGAACAAGAGCTCAACACATTAAAGCAAAAGAGTCCATCAGATCTGTGGAAGGAAGA
  TCTGGCTGTTTTTATTGAAGAACTGGAGGTTGTCGAGGCCAAGGAAAAGCAGGATGAACAAGTGGGCCTT
  CCTGGAAAGGCGGGGAAAGCAAAGGGGAAGAAAGCACAGATGTGTGCTGACGTCTTGCCTTCTCCTCGGG
  GCAAAAGAGTCATCCCCCAAGTGACTGTGGAGATGAAAGCTGAGGCAGAGAAGAAAATTAGGAAGAAAAT
  TAAGAGTGAAAACGTTGAGGGTACCCCTGCTGAGGATGGTGCGGAACCAGGAAGCCTCCGGCAACGAATA
  GAGAAGAAGCAGAAGAAAGAGCCAGGTGCAAAGAAGCAGACTACATTGCCGTTTAAGCCTGTCAAAAAAG
  GGAGGAAGAAAAACCCCTGGTCTGACTCTGAGTCAGACGTGAGCAGTAATGAAAGTAACGTTGATGTCCC
  TCCTCGACAAAAAGAGCAAAGAAGTGCTGCAGCAAAAGCCAAATTCACAGTGGATTTAGACTCGGATGAA
  GATTTCTCAGGTTTGGATGAGAAGGATGAGGATGAAGATTTCCTCCCATTAGATGCTACTCCACCTAAGG
  CCAAAATCCCCCCAAAAAATACTAAAAAAGCACTGAAGACCCAGGGAAGCTCCATGTCGGTAGTTGATCT
  TGAAAGTGATGTTAAGGACAGTGTGCCAGCTTCTCCAGGCGTTCCTGCTGCTGACTTCCCAGCGGAAACT
  GAACAGTCAAAGCCATCCAAAAAGACCGTGGGAGTGAAGAAGACAGCAACCAAAAGCCAGTCTTCAGTCT
  CCACTGCTGGTACCAAAAAGAGAGCTGCGCCAAAGGGAACCAAATCAGATTCAGCCTTGAGTGCTCGTGT
  CTCGGAAAAACCTGCTCCTGCCAAAGCCAAGAACAGTCGCAAAAGGAAGCCATCTTCTTCTGATAGCTCT
  GACTCTGATTTTGAGAGAGCAATTTCTAAAGGTGCCACGAGCAAGAAAGCAAAGGGAGAGGAACAGGACT
  TCCCTGTGGACTTGGAAGACACGATAGCTCCTCGAGCCAAATCTGACCGGGCGAGGAAGCCAATTAAGTA
  CCTGGAGGAGTCCGATGACGATGACGACCTCTTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_011623
• Insert Size: 4587 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011623.2, NP_035753.2
• RefSeq Size: 5217 bp
• RefSeq ORF: 4587 bp
• Locus ID: 21973
• UniProt ID: Q01320
• Cytogenetics: 11 62.91 cM
• Gene Summary: Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes. May play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation (PubMed:24321095).[UniProtKB/Swiss-Prot Function]