Nacad (NM_001081652) Mouse Untagged Clone

CAT#: MC224623

Nacad (untagged) - Mouse NAC alpha domain containing (Nacad), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nacad
• Synonyms: D230024G13Rik; mKIAA0363
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224623 representing NM_001081652
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGCCCTCCCTCTGGCGCTCCGCAGTGCTCGGCGCGCTGGCGCACGCTCACTCGGCTTGATAGGCG
  GGCGGGGCAGCGCTGACGTCATTTACGCCCCCCCCCCCCCCAGCAGAGGGGCGGTGGCCGTGCTGGCTAC
  TCCGAGCGCATTGACGGACAGAGGGACAGAGAGACAGGGGTATCGGCAGGCACACGTCCAGGCCATGCCA
  GGGGAGGCTGCGGGCGCTGAGCTGCCGCTGCCTGAGGCAGGAGGGCCTGGATCCCGCACAGATCACTCAT
  GTGATGCAGCTATTGCAACCATCCTGAAAGGGGACCAACTGGAGCCCCATGGCCTGACCCCGGGGCCTAG
  TCCTCTGGCCCTCACGTTCCTGTCCAGCAAGCCAGGTGCCCGGCCCCAGCCTGAGGGGGCCAGCTGGGAT
  GCAGGGCCCAGTGGAGCTGCCTCAGCCTGGGTGGACCCGGCAGAGGGCAGCCCAAGCCTAGTGGTCCTCC
  CCGAGGGCCTGCCTCTCCGGCCTGTGCCTGCCGAAGGCCCTCTCCCCACCACCCTGGAGCCCCGCATCGT
  GATGGGTGAGGAGACATGCCAGGTCATCGCTTCACCTAGGGCAGCCTGGCCAGTGCTCAGGGACCGGGAG
  GGTGGGCACCCTGCCCTGCACCCACCTCCAGAGCTGTGTTCTCAGGGCGATCCTCCTGTGCCTTCCCCTC
  CCCCTGACCTCGAGTCTTACTTCACACCCCCCTCTACTCCCACCAAGAGCACCCATGCCCTGCTTCCTGA
  CCATGGGCCCCACAGGGATGCCTGGGATCTGGAGGCCGAGCTGCTGGATGAGTTATTGGACTCCACGCCA
  GCCTCACCCTCCGGCTCCTACATTACAGCAGACGGGGACAGCTGGGCTTCATCCCCTTCCTGTTCCCTCA
  GCCTGCTGGACCCAGCTGAAGGGCTGGACTTTCCCTCCGACTGGGGCCTTTCCCCATCAGGGTCAGTGGC
  AGATGACCTAGAGCCACACCCCGCAGCACCTCCAGAGCCCCCATCCTCTGAGTCTAGCCTCTCAGCAGAC
  AGCAGCTCTTCCTGGAGCCAAGAGGGCCATTTCTTTGACCCAAACTTCCTGGCCAACGACCCGATGATCC
  CTGCCGCCCTACTACCCTTCCGGGGTAGCCTCATCTTCCAGGTGGAGGCTGTGGAGGTGACACCATTGCC
  CCAAGAGGAGGAGGAAGACGAAGAGGATGTAGCTGCTACTGCTGCTGCTGCTGCTCCTGCTGCTGCAACC
  CCAGATGGAGACCTGGCTGGGGAGGGGGAGGATGACAGCACGTCTGCCTCCTTCCTGCAGTCGCTGTCCG
  ACCTGTCCATCATCGAGGGCATGGATGAGGCCTTCGCCTTCCGGGATGACACCTCCGCAGCCTCCTCTGA
  CTCAGACTCGGCTTCCTATGCTGGGGCAGATGATGACAGGCTGTACAGTGGGGAGCCCCATGCCCAGCCC
  AGTGCTCAGAATACAGAGCAGGCCTACAGAAGTAGGGCTACATTCCCAGGGATAGAGTCCACACCTCAGA
  CATCAGAGCAAGAGATCTGTCTTACCAATAGCCAGGAATCAGTTGCAGAAATAGCAGAAGAGATCCTAAC
  TCTAGGCCTAGAGTCTGAAGCTATGAGGACACCTCCTGATCAGCAAGCAGCTCCAGGCCCCCAGGTGGAG
  GAAACTCCCACAGTGACCCCTTGGGTGGGGAACAAAGTTGACCTGGTTGTAGAACAAGTATCCAAGGCTC
  TTCCTGAGCCCTGCCAGGAGGGGATAAGCACCACCTTAGGCTGTAAGCCCCTCACTGCAGAGGCAATTCC
  AGATCTGCAGGAAGGAGCAAGCCCCAGCTTGTGTCCAGTCCTTCCTGAGAAGAAGGAAGAAGGCCAGGGC
  CTTCCCTCGACACTGGAGTATGTGGCTGTGGCTTTAGAGGGGCCCTGGAAGGCTGAGGGAGGTGTCACAA
  TACCGCAGGACCCCCTTATGACATTGCCCCCACTCTTGCAAAGTACAGTTCCCACCTCAGGCCCAGAGTC
  TGTGGCGGTAGTCGCACTAGAGCCCCAGCAGAATGAAGGGTGTGTCACAGTACTCCCTGATGTCCCCGTG
  GTATTACCTCCATCCCCACAAAGTGTAGATCCAAGCTCAGGTCCAGAGGCTATGGCCGTGGCTACATATG
  AGTTCCAGCGGGCTAAGGAAGGTACCCCAGGACTCCAGGATTCCCCTGTGGCCGCATCCCCAGCACTGCA
  AGGCCCAGATCCCACCTCAGAGCCAGAACCTGAAGTTGTGGTCACCTCGAGGTCCCAGCAGGATGAAGGG
  ATTGTCACAGTACCCCAGGAGTCTCCTACAGCATCATCTCTAACCCTACAAAGCTCACATCCCACTTCGG
  ACCAAGAACGCGAGGTTGCTGCCACACTGGGACCACAGCAGGCTGAGGAGGGCGTCACCATACCCCAGGT
  TGCCCCAGTAGCATCACCTTCATTGCTGCAAGGCTTAGAGTCAACCTCAGACCTAGAATCTGTGGTTGTA
  GGCACACCGGAGTCCCAGCAGGATGAAGGGATTGCCACAGCAACCCAGGACACCCCTGTGATGGCACCTC
  CACCCCTTCGAGGTACAGATTCCACCTCGGACCCAGAGCTGATAGCTCCAGACACCTCTCAGGCCCTGCA
  GAGAGAAGCAGGCCACACCCCAGGGACCAAGCCTTCTGTCTCTGAGGCCCATCAGGAATTGGGTGTGGCC
  TCAGGACCAAGGCCAGTGCCTAAGGAGGGGGATGCAGAGCCTCCTCCACACTCAGCACCCCCAGCTTCAA
  ACCAGGCCCAACAGAATGGCTCAGAGCCAGGTTACAAGAGTGACAGCTTTGGGGCTCCAGAAGAGTCAGA
  TTCCACTTTGAGCACAAAGACCTCTGAGCCCACATCGTGCATGGGGGAGAAAGTAGCTGCGAACATGTCT
  GCACCCAAGCAAGGAGCATGTCTTGAAGCCCATGATGGGGTCAAGACCCACTCACCTCAAAGAGAGGCCT
  TGAGGTCCAAGAACAAGCGTGGTAGAGGCACCAAATCACCAGGGCAGGGAAATGGGCCCAAGTCAGCAAC
  ATCCCAAGGTGCCGTGGAGACTTGCAGGGCACATTCTGCTGCTCGCTCTGAGGTCAGCCAGCCACAGCTG
  AGAAGCAACGAGGACACCTCAGGCCCCAGGCTGCCTGTGGCTGTATCTGTACAAGCCAGGCTAGGTTCCT
  GCCCAGGGTCCCCAGCGAGAGCCACATGCACACTGAGCAGGGTCTACGCTGAAGAGACTAGCAGGTGTGC
  ACCACCCTTCCAGCATCTGGAGCCTATGCTGGGCCTGGGCAGTGCTGAACAGCCCAAGGTGACCCCTGGT
  ATCCTTAATCTTTCCCCAGACAACTCTGCTGGTGACCTGCTCACCACATCTCAGAATAGGTTTCTGGACC
  CTGACCCTGCTCCCAGTACCCTCGACAGGGCATCCCAATCATCCCCGGGGCCTCCAGACCCCTGCCTGTG
  CCCCCCTCCCCAGAAAGCCTCTGAGGAAGAAGAGAAGCCCCCAGCCTCGCGTGGCCCGATGCCTCGGGCA
  GGAGCCCAGGGAGCTGCCGCCATCACCACCTCAGGATCCACAAAGCCTCCGGGAGCCCGGCAGCGTGTCA
  GCCTCTCACCTCACTCCACCCTCAACCCCAAGGTGGCCCCCACAGATACCAAAGACCTGGCTTGCATCAT
  CTCAAGCCCCTGCCAAGTGCCTCCTCCCTCTGGGACCCAGAACCCATCAGGGCCTCGAGAGTTCCCAGCT
  CTTGAACAAAAGGATGAGGACAGCCTGGAGGAAGACGCGCAGAGGGCCCCAGGCTCCGGCCAGCGTTGGG
  AGAGCCACGGGGAGTCGTCCAGTGAGCTGGATGAGTACCTCGCCCCTCCCCCAGACGCGCAGAGGACCCC
  AGGCTCCGGCCAGCGTTCGGAGAGCCACGGGGAGTCGTCCAGTGAGCTGGGTGAGCAGGATCTCTCACCT
  CAGAAATCGCAGTGCCCGGCACAGGGCCCGGCAGGCAGCAACGAGGAGACCATTGCCAAAGCCAAGCAAA
  GCCGCAGTGAGAAGAAGGCTCGAAAGGCAATGTCAAAACTAGGCTTGCGTCAGATTCAAGGAGTCACCAG
  GATCACCATCCAGAAGTCCAAGAATATCTTGTTTGTCATTGCCAAGCCGGATGTCTTCAAGAGCCCCGCC
  TCAGACACCTACGTGGTCTTTGGCGAGGCCAAGATCGAGGACCTGTCCCAGCAAGTACACAAAGCTGCAG
  CAGAGAAGTTCAAGGTGCCCTCAGAGCCCTCAGCCCTGGTCCCTGAGCTGTCACCTGGGCCCCGTGTGAG
  GCCAGAGTGTGAGGAGCAAGAGGAGGAAGATGAGGAGGTAGAGGAGGCTGGACTAGAACCTCGTGACATT
  GAACTGGTGATGGCCCAGGCCAATGTGACCCGGGCAAAGGCTGTGCGGGCCCTGAAGGACAACCACAGTG
  ATATTGTCAATGCTATCATGGAACTGACAATGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001081652
• Insert Size: 4515 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081652.1, NP_001075121.1
• RefSeq Size: 4914 bp
• RefSeq ORF: 4515 bp
• Locus ID: 192950
• UniProt ID: Q5SWP3
• Cytogenetics: 11 A1
• Gene Summary: May prevent inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). May bind to nascent polypeptide chains as they emerge from the ribosome and block their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. May also reduce the inherent affinity of ribosomes for protein translocation sites in the ER membrane (M sites) (By similarity).[UniProtKB/Swiss-Prot Function]