Atp10a (NM_009728) Mouse Untagged Clone

CAT#: MC224614

Atp10a (untagged) - Mouse ATPase, class V, type 10A (Atp10a), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Atp10a
• Synonyms: Atp10c; pfatp
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224614 representing NM_009728
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCGGGAGCTCCCGGCGGCCGAAGAGTCCGCATCCTCGGGATGGCGGCGGCCGCGAAGAAGGCGCT
  GGGAGGGCAGGACGCGCACGGTGCGCTCCAACCTGCTACCGCCTCTGGGCACCGAGGACTCCACGATCGG
  AGCCCCCAAGGGCGAGCGGCTGCTGATGCGCGGCTGCATCCAGCACCTGGCCGACAACCGCCTCAAGACC
  ACCAAGTACACGCTGCTGTCCTTCTTGCCCAAGAATCTCTTCGAGCAGTTCCACCGCCTAGCCAATGTGT
  ACTTCGTCTTCATCGCGCTGCTCAACTTTGTGCCGGCTGTGAACGCCTTCCAGCCGGGCCTGGCACTGGC
  GCCTGTGCTCTTCATTCTGGCGGTCACAGCCATCAAAGACCTGTGGGAGGACTACAGCCGACACCGCTCG
  GACCATGAAATCAACCACCTGGGCTGCCTTGTCTTCAGCAGGGAAGAAAAGAAGTATGTGAATCGATACT
  GGAAAGAAATCCGAGTGGGCGACTTTGTCCGTCTTTGCTGCAATGAAATCATACCTGCGGACATTTTGCT
  GCTGTCATCCAGTGACCCTGACGGGTTGTGCCACATCGAGACTGCTAACCTGGATGGAGAGACCAACCTG
  AAGCGGCGCCAAGTGGTCCGTGGCTTCTCGGAGCTTGTTTCTGAATTCAACCCTCTGACGTTCACCAGTG
  TAATCGAGTGTGAGAAGCCAAACAATGACCTGAGTAGGTTTCGTGGCTACATCATGCACAGCAATGGGGA
  GAAGGCTGGGCTGCATAAGGAAAACTTGCTGCTTCGTGGGTGCACCATCAGAAACACAGAGGCTGTGGCT
  GGAATTGTCATCTACGCAGGACATGAAACCAAAGCCCTGCTGAACAATAGTGGGCCCCGCTACAAGCGCA
  GCCAGCTTGAAAGGCAGATGAACTGCGATGTCCTCTGGTGTGTCCTTCTCCTCGTTTGTATATCTCTATT
  TTCAGCGGTTGGGCATGGATTGTGGGTTCGGCGCTATCAAGAGAAAAAAGCACTATTTGATGTTCCCGAG
  TCTGATGGCAGCTCTTTATCCCCAGCCACAGCTGCAGTTTACTCATTTTTTACAATGATAATAGTTCTGC
  AGGTTTTGATTCCAATTTCCTTATATGTTTCCATTGAAATTGTTAAAGTGTGCCAAGTATACTTCATTAA
  CCAAGATATAGAGCTCTATGATGAAGAGACAGATTCACAGCTGCAGTGCCGAGCTCTGAACATAACAGAA
  GACTTAGGGCAGATAAAATACATCTTCTCGGACAAAACCGGCACATTAACTGAGAACAAGATGGTCTTTC
  GAAGATGCACTGTGTCCGGCATAGAATATTCTCACGATGCCAATGCTCAGCGACTGGCCAGGTATCAAGA
  GGCAGACTCAGAAGAGGAGGAAGTGGTGTCCAAGGTGGGCACAATATCCCACCGTGGCAGCACTGGGAGC
  CATCAGAGCATCTGGATGACTCATAAGACCCAAAGTATCAAGTCCCACCGGCGCACAGGCAGTCGAGCTG
  AGGCAAAGAGAGCCAGCATGCTGTCTAAGCACACTGCCTTCAGCAGCCCCATGGAGAAAGACATTACACC
  TGACCCCAAGCTGTTGGAGAAGGTGAGTGAGTGTGACCGATTCCTCGCCATTGCAAGGCACCAGGAACAC
  CCTCTGGCCCATCTCTCACCCGAGCTGTCTGATGTTTTTGATTTCTTCATCGCACTCACCATCTGCAATA
  CAGTAGTTGTCACCTCTCCAGACCAACCACGACAAAAGGTGAGGGTGAGGTTTGAGCTGAAGTCCCCAGT
  AAAGACCATAGAAGATTTCCTTCGGAGGTTCACGCCCAGCCGCCTGGCTTCTGGCTGCAGCAGTATCGGG
  AACCTGAGCACCAGTAAATCCAGCCACAAATCTGGATCAGCTTTTCTGCCCAGCCTGTCTCAGGACAGCA
  TGCTTCTTGGGCTGGAGGAGAAGCTGGGTCAAACAGCACCGTCCATTGCCAGCAATGGCTATGCCAGCCA
  GGCAGGCCAGGAGGAAAGCTGGGCCTCAGAGTGTACTACAGACCAGAAGTGTCCAGGGGAGCAGAGGGAG
  CAGCAGGAGGGGGAGCTGCGATATGAGGCTGAGAGCCCAGATGAGGCTGCACTGGTGTATGCAGCCAGAG
  CCTATAACTGTGCCCTGGTGGACCGGCTTCACGACCAGGTGTCTGTGGAGTTGCCACACCTAGGCAGGCT
  CACCTTCGAGCTGCTGCACACACTTGGCTTCGACTCCATCCGCAAGAGGATGTCAGTGGTGATCCGCCAC
  CCGCTGACTGACGAGATCAATGTATACACCAAGGGGGCCGATTCGGTGGTCATGGATCTTCTGCTGCCCT
  GTTCCTCAGATGACGCCAGAGGAAGGCACCAGAAAAAGATCCGGAGTAAAACACAGAATTACCTCAATCT
  TTATGCAGTAGAAGGCCTGCGCACCCTGTGCATTGCCAAGAGGGTTCTGAGCAAAGAAGAGTATGCCTGC
  TGGTTGCAGAGCCACATAGAAGCCGAGGCGTCTGTGGAGAGCCGTGAGGAGCTCCTCTTCCAGTCTGCCG
  TTCGCCTGGAGACGAACTTGCACCTGCTGGGTGCCACGGGGATAGAAGACCGCCTTCAGGAAGGAGTACC
  TGAAACCATTGCCAAATTACGACAAGCTGGCCTGCAGATTTGGGTTCTCACAGGTGACAAACAGGAAACA
  GCCATTAACATTGCATATGCCTGCAAACTGCTGGATCATGGTGAAGAGGTCATCACACTGAATGCTGACT
  CTCAGGAAGCATGTGCAGCCCTGCTGGACCAGTGCCTCAGTTATGTGCAATCCAGAAACCCTAGAAGTAC
  CCTTCAGAACTCTGAAAGCAACTTGAGTGTGGGGTTCTCTTTCAACCCAGTCTCCACATCCACTGACGCC
  AGCCCCAGCCCAAGCCTTGTGATTGATGGAAGAAGTCTGGCCTATGCCCTCGAGAAAAGCCTGGAGGACA
  AATTTCTTTTCCTTGCCAAGCAGTGTCGCTCAGTCCTCTGCTGCCGATCGACCCCTTTGCAAAAGAGCAT
  GGTGGTGAAGCTTGTCAGAAGCAAGCTCAAGGCCATGACCTTGGCCATAGGTGATGGGGCCAATGATGTC
  AGCATGATCCAGGTGGCAGATGTAGGTGTTGGGATCTCAGGCCAGGAGGGCATGCAGGCTGTGATGGCAA
  GTGACTTTGCAGTGCCAAGATTCCGATACCTGGAGAGACTTTTGATCGTCCATGGACACTGGTGCTACTC
  CAGACTGGCCAATATGGTGCTGTATTTCTTCTATAAAAACACAATGTTTGTGGGTCTCTTGTTTTGGTTC
  CAGTTTTACTGTGGCTTTTCTGCATCTGCCATGATTGATCAGTGGTATCTGATTTTCTTTAATCTGCTCT
  TCTCATCACTTCCCCAGCTGGTGACTGGGGTGCTGGATAAAGATGTTCCAGCTGACATGCTGCTGAGAGA
  GCCACAGCTTTACAAGAGTGGCCAAAACATGGAGGAATACAGACCACGAGCATTCTGGTTAAACATGGTG
  GATGCTGCCTTTCAAAGCCTGGTATGCTTTTTCATTCCCTACCTGGCCTACTATGACTCCGATGTGGATG
  TCTTTACCTGGGGGACCCCTGTCACAGCAATAGCACTGTTCACCTTCCTGCTGCACCTGGGTATTGAAAC
  CAAAACCTGGACCTGGCTCAACTGGTTAGCCTGTGGCTTCAGTACCTTTTTGTTTTTCTCCGTGGCTCTG
  ATTTACAACACTTCCTGTGCCACATGCTACCCTCCATCTAACCCTTACTGGACCATGCAGACATTACTGG
  GTGACCCTCTGTTCTACTTGACTTGTCTCATCGCACCTATTGCTGCATTGTTGCCCAGATTGTTTTTCAA
  AGCCCTCCAGGGGAGTCTTTTCCCCACCCAACTCCAGCTAGGACGCCAGTTGGCCAAGAAACCCCTCAAC
  AAATTCAGTGATCCCAAAGAGACTTTTGCTCAGGGACAGCCACCTGGACACTCAGAAACCGAGCTTTCAG
  AAAGGAAGACCATGGGACCTTTTGAGACTCTCCCTAGAGACTGTGCCTCTCAAGCTTCCCAGTTCACACA
  GCAGCTGACCTGTTCCCCAGAAGCCAGCGGGGAACCCAGTGCAGTGGATACAAACATGCCATTAAGAGAG
  AACACCTTGCTGGAAGGACTTGGCTCTCAGGCCTCAGGGTCCTCCATGCCCAGAGGGGCTATTTCAGAAG
  TATGTCCTGGGGATTCTAAGAGACAATCCACCAGTGCTAGTCAGACTGCCCGTTTGTCGTCACTCTTCCA
  TCTGCCCAGCTTTGGCTCCCTAAATTGGATCTCCTCGCTGTCACTGGCCAGTGGGCTGGGAAGTGTCTTG
  CAATTGTCTGGAAGTAGTTTACAGATGGATAAGCAGGATGGTGAGTTTCTATCCAACCCACCACAGCCAG
  AGCAGGACCTGCACAGCTTCCAGGGACAAGTCACGGGCTACTTATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009728
• Insert Size: 4527 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009728.2, NP_033858.2
• RefSeq Size: 5469 bp
• RefSeq ORF: 4527 bp
• Locus ID: 11982
• UniProt ID: O54827
• Cytogenetics: 7 B5
• Gene Summary: Catalytic component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids from the outer to the inner leaflet of various membranes and ensures the maintenance of asymmetric distribution of phospholipids. Phospholipid translocation seems also to be implicated in vesicle formation and in uptake of lipid signaling molecules (By similarity).[UniProtKB/Swiss-Prot Function]