Arhgap35 (NM_172739) Mouse Untagged Clone

CAT#: MC224608

Grlf1 (untagged) - Mouse glucocorticoid receptor DNA binding factor 1 (Grlf1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Arhgap35
• Synonyms: 6430596G11Rik; AI841135; Grlf1; mKIAA1722; p190A; p190RhoGAP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224608 representing NM_172739
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGATGGCAAGAAAGCAAGATGTCCGCATCCCCACCTACAACATCAGTGTGGTGGGATTGTCAGGCA
  CTGAGAAGGAGAAAGGCCAGTGCGGCATTGGGAAGTCTTGTCTGTGTAACCGCTTTGTGCGCCCAAGTGC
  TGATGAGTTTCACCTGGACCACACTTCTGTCCTTAGCACCAGTGACTTTGGTGGGCGGGTGGTCAATAAT
  GACCACTTTCTGTACTGGGGAGAAGTTAGCCGCTCCCTGGAGGATTGTGTGGAATGTAAGATGCACATTG
  TGGAGCAGACTGAGTTTATTGACGATCAGACTTTTCAACCTCATCGAAGCACGGCACTGCAGCCCTACAT
  CAAGAGAGCCGCAGCCACCAAGCTTGCTTCAGCTGAAAAACTCATGTATTTTTGCACTGACCAGCTGGGA
  CTGGAGCAAGACTTTGAGCAGAAACAGATGCCAGATGGGAAGCTGCTGGTTGACGGTTTCCTTCTGGGCA
  TTGATGTTAGCAGGGGCATGAACAGGAACTTCGATGATCAGCTCAAATTTGTCTCCAATCTCTACAATCA
  GCTTGCAAAAACAAAAAAGCCCATAGTGGTAGTCCTGACTAAGTGTGATGAGGGCGTTGAGCGGTACATT
  AGAGATGCACATACTTTTGCCTTAAGCAAAAAGAACCTCCAGGTTGTAGAGACCTCTGCAAGGTCCAATG
  TGAATGTGGACTTGGCTTTCAGCACCTTAGTGCAACTCATTGATAAGAGTCGAGGGAAGACAAAAATCAT
  CCCTTACTTTGAAGCTCTCAAGCAGCAGAGTCAGCAGATAGCTACAGCAAAGGACAAGTATGAGTGGTTA
  GTGAGCCGCATTGTGAAAAATCACAATGAGAACTGGCCGAGTGTCAGCCGAAAGATGCAGGCCTCCCCTG
  AGTACCAGGACTATGTCTACCTGGAAGGGACACAGAAGGCCAAGAAGCTCTTCCTGCAGCACATTCACCG
  CCTCAAGCATGAGCATATTGAGCGTCGGAGAAAGCTGTACCTGGCAGCTCTGCCATTGGCTTTTGAAGCC
  CTCATACCTAATCTAGATGAAGTGGACCACCTGAGCTGTATTAAAGCAAAAAAGCTGTTAGAGACCAAGC
  CAGAGTTCTTAAAGTGGTTTGTTGTACTTGAAGAAACACCATGGGATGCCACCAGCCACATTGACAACAT
  GGAAAATGAGCGGATTCCCTTTGACTTGATGGATACTGTCCCTGCTGAGCAGTTGTATGAGACCCACTTG
  GAGAAGCTGAGGAACGAGAGGAAGAGAGCTGAGATGCGAAGGGCTTTCAAAGAAAACCTGGAGACCTCTC
  CTTTCATAACTCCTGGGAAACCTTGGGAAGAAGCTCGTAGTTTTATTATGAATGAAGACTTCTACCAGTG
  GCTGGAAGAATCTGTGTACATGGACATCTATGGCAAGCACCAAAAGCAGATTATAGACCGAGCAAAGGAA
  GAGTTCCAAGAGTTGCTTTTGGAGTACTCAGAATTGTTTTATGAGCTGGAGCTGGACGCTAAGCCCAGCA
  AGGAGAAGATGGGTGTCATCCAGGATGTTCTGGGCGAAGAGCAGAGATTTAAAGCATTGCAGAAGCTCCA
  AGCAGAGCGTGATGCCCTCATTCTGAAGCACATCCATTTTGTGTACCACCCGACGAAGGAGACATGCCCA
  AGCTGTCCAGCTTGTGTAGATGCTAAGATTGAACACTTGATCAGTTCTCGCTTTATCAGACCATCTGATC
  GGAATCAGAAGAACTCTCTGTCTGACCTCAATATTGATAGGATCAATTTGGTTATCTTAGGCAAAGATGG
  CCTTGCCCGAGAGTTGGCCAATGAAATTCGAGCTCTTTGTACAAATGATGACAAGTATGTAATAGATGGT
  AAAATGTATGAGCTTTCTCTGAGGCCAATAGAGGGGAATGTCCGGCTTCCTGTGAACTCTTTCCAGACAC
  CAACCTTCCAACCCCATGGCTGTCTCTGCCTTTACAATTCAAAGGAGTCACTGTCCTATGTGGTGGAGAG
  TATAGAGAAGAGCAGAGAGTCTACACTGGGCAGGCGGGATAATCATTTAGTCCACCTTCCCTTGACCTTA
  ATTTTAGTTAACAAGAGAGGGGACACAAGTGGAGAGACTCTGCACAGCTTGATACAGCAAGGTCAGCAGA
  TTGCCAGCAAACTTCAGTGTGTCTTTCTTGATCCGGCTTCTGCTGGCATTGGTTATGGACGCAACATTAA
  TGAGAAGCAGATCAGTCAAGTTCTGAAAGGACTCCTGGACTCCAAGCGCAACTTAAACCTGGTTAGCTCC
  ACTGCAAGTATCAAAGATTTGGCTGATGTGGACCTTCGAATTGTCATGTGTCTGATGTGTGGTGATCCCT
  TTAGTGCAGATGACGTTCTCTCTCCTGTCCTGCAATCCCAAACTTGTAAATCTTCCCACTGTGGGAGCAG
  CAACTCTGTTTTACTTGAACTTCCAATTGGACTACACAAGAAGCGCATTGAGCTGTCTGTTCTTTCATAC
  CATTCCTCATTTAGCATCCGAAAGAGCCGGTTGGTTCATGGGTACATTGTTTTTTATTCAGCCAAACGTA
  AGGCCTCCTTGGCAATGTTACGTGCCTTTCTTTGTGAAGTGCAGGATATTATCCCCATCCAGCTTGTCGC
  ACTCACTGATGGCGCTATAGATGTCCTAGACAATGACTTAAGTCGAGAGCAGCTAACAGAGGGAGAGGAA
  ATTGCACAAGAGATTGACGGGAGATTCACAAGCATCCCTTGTAGCCAGCCCCAGCATAAACTTGAGCTCT
  TTCATCCCTTTTTTAAAGATGTGGTGGAGAAAAAGAACATAATTGAGGCCACACATATGTATGATAATGT
  AGCTGAGGCCTGCAGCACCACTGAGGAAGTGTTCAACTCCCCCAGAGCTGGCTCACCCCTCTGCAACTCA
  AACTTACAGGACTCAGAAGAAGATGTGGAGCCTCCATCATACCACCTGTTTCGGGAAGATGCGACATTGC
  CCTCCCTGTCCAAAGATCATTCCAAGTTCTCTATGGAACTGGAGGGAAATGACGGGCTATCTTTCATAAT
  GAGCAATTTTGAGAGTAAACTGAACAACAAAGTACCTCCCCCAGTCAAACCAAAGCCTCCTGTGCATTTT
  GATATCACAAAAGATCTTTCTTACTTAGACCAAGGCCATCGGGAGGGACAGAGGAAATCTATGTCTTCTA
  GCCCCTGGATGCCTCAGGATGGGTTTGATCCTTCTGACTACGCAGAGCCCATGGATGCTGTGGTCAAGCC
  AAGGAATGAGGAAGAAAACATATACTCGGTGCCCCACGACAGCACCCAGGGCAAGATCATTACCATTCGG
  AACATCAACAAAGCCCAGTCCAATGGCAGTGGTAATGGTTCTGACAGTGAGATGGACACAAGTTCCCTAG
  AGCGAGGCCGCAAGGTATCTGCAGTGAGTAAGCCTGTGCTGTACAGGACAAGATGCACTCGCCTGGGGCG
  GTTTGCTAGTTACCGTACCAGCTTCAGTGTCGGGAGTGATGATGAGCTGGGACCCATCCGAAAGAAAGAG
  GAGGACCAGGCATCCCAAGGTTATAAAGGGGACAATGCTGTCATTCCATATGAAACAGATGAGGACCCCA
  GGAGGAGGAATATCCTTCGAAGTCTAAGAAGGAACACCAAGAAACCAAAGCCCAAACCCCGGCCATCCAT
  CACAAAGGCAACCTGGGAGAGTAACTATTTTGGGGTGCCCTTAACAACAGTGGTGACTCCAGAGAAACCG
  ATACCCATTTTCATTGAAAGATGCATTGAGTACATCGAAGCCACAGGACTAAGCACTGAAGGCATCTACC
  GGGTCAGTGGAAACAAGTCAGAAATGGAAAGTTTGCAAAGACAGTTTGATCAAGACCACAATCTGGACCT
  GGCAGAGAAAGACTTCACTGTGAACACTGTGGCAGGGGCCATGAAGAGTTTTTTCTCAGAGCTACCAGAC
  CCCCTGGTACCATACAGCATGCAGATTGACTTGGTGGAAGCTCACAAAATCAACGACAGGGAACAGAAGC
  TGCATGCTCTGAAGGAAGTGCTGAAGAAGTTCCCGAAGGAAAACCATGAAGTCTTCAAATATGTCATCTC
  CCACCTGAACAAAGTCAGCCACAACAACAAGGTGAATCTTATGACCAGTGAGAACCTGTCCATCTGCTTC
  TGGCCCACGCTGATGCGGCCTGACTTCAGCAGCATGGACGCGCTCACAGCCACGCGCTCCTACCAGACCA
  TCATCGAGCTCTTCATCCAGCAGTGCCCCTTCTTCTTCTACAACCGGCCCATCAGCGAGCCTCCAGGGGC
  TGCGCCTGGCTCCCCTTCAGCCATGGCTCCCACTGTTCCCTTCCTCACCTCTACACCTGCTACCAGTCAG
  CCATCACCTCCCCAGTCACCTCCTCCAACCCCTCAGTCCCCAATGCAGCCATTGCTGTCCTCTCAGCTCC
  AAGCCGAACACACGCTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_172739
• Insert Size: 4500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172739.4, NP_766327.3
• RefSeq Size: 8393 bp
• RefSeq ORF: 4500 bp
• Locus ID: 232906
• UniProt ID: Q91YM2
• Cytogenetics: 7 A2
• Gene Summary: Rho GTPase-activating protein (GAP). Binds several acidic phospholipids which inhibits the Rho GAP activity to promote the Rac GAP activity (PubMed:16971514). This binding is inhibited by phosphorylation by PRKCA (By similarity). Involved in cell differentiation as well as cell adhesion and migration, plays an important role in retinal tissue morphogenesis, neural tube fusion, midline fusion of the cerebral hemispheres and mammary gland branching morphogenesis (PubMed:11044403, PubMed:11283609, PubMed:18502760, PubMed:21945077). Transduces signals from p21-ras to the nucleus, acting via the ras GTPase-activating protein (GAP) (PubMed:16971514). Transduces SRC-dependent signals from cell-surface adhesion molecules, such as laminin, to promote neurite outgrowth. Regulates axon outgrowth, guidance and fasciculation (PubMed:11283609). Modulates Rho GTPase-dependent F-actin polymerization, organization and assembly, is involved in polarized cell migration and in the positive regulation of ciliogenesis and cilia elongation (PubMed:11044403, PubMed:26859289, PubMed:18502760). During mammary gland development, is required in both the epithelial and stromal compartments for ductal outgrowth (PubMed:21945077). Represses transcription of the glucocorticoid receptor by binding to the cis-acting regulatory sequence 5'-GAGAAAAGAAACTGGAGAAACTC-3'; this function is however unclear and would need additional experimental evidences (By similarity).[UniProtKB/Swiss-Prot Function]