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Gemin5 (NM_001166669) Mouse Untagged Clone

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SKU
MC224603
Gemin5 (untagged) - Mouse gem (nuclear organelle) associated protein 5 (Gemin5), transcript variant 1, (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
  $1,415.00
7 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Gemin5
Synonyms AA407055; AA407208; AI451603; BB194447; C330013N08
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC224603 representing NM_001166669
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGCCGGAGCCGCGGACGCTGCCGCCGTCCCCCAACTGGTACTGCTCACGCTGCAGCGACGCGGCGC
CCGGAGGCATCTTTGGCTTCGCTGCGCGGACCTCCGTCTTCCTCGTCCGCGTGGGTCCGGGTGCGGGCGC
GAGCCCAGGGGCGCCCCCGTTTCGAGTGGTAGGAGAGTTGGTGGGACACACTGAAAGAGTATCTGGTTTC
ACTTTTTCTCATCACCCTGGACAATACAACCTCTGTGCCACCAGCTCAGACGATGGAACTGTGAAGGTTT
GGGATGTAGAGACCAAGACTGTAGTGACAGAACACACACTCCATCAGCATACAATATCAGCACTGCATTG
GTCTCCGACAGTTAAGGACTTAATAGTCTCTGGGGATGAAAAAGGAGTCGTTTTCTGTTACTGGCTTAAC
AGAAATGACAGCCAGCACCTCTTCACAGAGCCCAGGACCATATTCTGTCTGACGTGTTCACCTCATCACG
AAAATCTAGTAGCCATTGGCTACAAAGATGGCATAGTGGTTATCATTGACATCAGTAAAAAGGGAGAAGT
TATTCACAGACTTCGAGGCCACGATGACGAGATCCACTCCATAGCCTGGTGTCCTCTGTCTGGTGAAGAC
TGCTTATCCATCAGCCAAGAGGAAAACTCGGAAGAACCTGACATTCCCAATGGGAAGCTTATAGCAGAAA
CCCCCATCACAAAAGGCTGCTACTTAGCCACAGGAAGCAAAGATCAGACCATTCGAATCTGGAGCTGTTC
CCGCGGCCGGGGTGTAATGGTTTTGAAATTGCCCTTCCTGAAGAGAAGAAGTGGGGGTGTGGACCCAACA
GTTAAAGAACGGCTTTGGTTGACACTTCATTGGCCTAAGAACCAACCAACACAGCTGGTATCCAGTTGTT
TTGGAGGTGAGCTGCTGCTATGGGACCTCACCCAGTCATGGAGGCGGAAGTACACGCTCTTCAGCACCTC
AGCAGAAGGGCACAATCATTCCAGAATTGTCTTCAATCTGTGTTCTCTGAAGACTGAAGATGGCAAGCAG
CTGCTACTGTCCACATCCATGGATAGAGATGTAAAATGTTGGGACATGGCCACCCTGGAGTGTTGCTGGA
CATTGCCTTCTCTTGGAGGTTTTGCCTACAGCCTGGCCTTTTCTCCTGTGGACGTGGGCTCCCTGGCCAT
AGGTGTTGGGGACGGCATGATCCGGGTGTGGAATACACTCTCCATAAAGAACAACTACGACGTGAAAAAC
TTCTGGCAAGGAGTCAAGTCCAAGGTCACAGCGCTATGCTGGCACCCAAACAAGGAAGGCTGCTTAGCCT
TCGGGACCGATGACGGAAAAGTGGGATTGTATGACACCTGCTCCAACAAACCTCCACAGATTTCAAGCAC
GTATCATAAGAAGACCGTGTATAGGTTGGCTTGGGGGCCACCAGTCCCCCCCATGTCACTTGGAGGGGAA
GGAGACAGGCCTTCCCTCACCTTGTACAGCTGTGGAGGGGAAGGCGTTGTGCTACAGCATAACCCCTGGA
AGCTCAGTGGAGAGGCCTTCGACATCAACAAACTCGTGAGGGACACCAACTCCATTAGATACAAACTGCC
CGTGCACACAGAGATCAGCTGGAAAGGAGATGGCAAAGTCCTGGCTCTTGGGAATGAAGATGGATCAATA
GAAATATTCCAAGTCCCCAACTTGAGGCTGCTGTGCACCATCCAGCAGCACCACAAGCTTGTGAACGCCA
TAGTCTGGCACCATGAGCATGGCAGCCGTCCAGAGCTGAGCTGTCTCCTTGCTTCTGGCTCCAACAATGC
AGTCATCTACGTGCACAACCTGAAAGCAGTCCTAGAAAGCAATCCTGAGTCTCCAATAACCATCACAGAG
CCCTACCGCACCCTCTCGGGGCACACAGCCAAGATTACCAGTCTGGCATGGAGCCCACATCATGACGGAA
GGCTGGTATCTGCTTGCTACGATGGCACAGCTCAGGTGTGGGATGCCCTCCGGGAAGAACCTTTGTTCAA
TTTCCGAGGACATCGAGGCCGGCTGCTATGTGTCGCGTGGTCCCCAGTGGATCCAGAATGCATCTACTCA
GGTGCAGATGACTTCTGTGTCTACAGGTGGCTGACTTCCATGCAGGACCATTCCCGGCCTCCTCAAGGGA
AAAAATGTATTGAACTAGAGAAAAAACGACTCTCTCAATTTAAGCCAAAGCTTAAAAAGAAGAAAAAACC
TACCTTGCGACTGCCTGTAAAGCAGGATTCATCCGTTGGCAATGAAGATGAGAGTGTAAAGGAGAACTCT
GGACCTGCTGAGAACGGCTTGTCGGACCAGGATGGTGAGGAGGAAGCCCAGGAGCCAGAGCTGCCTCCCT
CTCCTGTAGTTTGTGTAGAACCTGTTTCCTGTACTGACATTTGCTCAGGCTTTGAAAAGTCAAAAGTCAC
TGTTAGTAGCAAAGCCACCTCACTGAAAAAGGAGCCAGCTAAAGAGAAACCAGAAGCCTTGCTCAAGAAG
AGGAAGGCTCGCTCCATGCTCCCTCTGAGCACCAGCTTAGACCACAGGTCCAAAGAGGAGCTCCATCGAG
ACTGCTTGGTACTAGCAACTGCAACACACGCCAAAGCAGAGCTAAATGAAGATGTGTCTGCCGACCTTGA
GGAACGATTCCACTTGGGCCTTTTCACAGACAGGGCTACTCTATACAGGATGATGGAAACAGAAGGGAAA
GGTCACTTGGAAAGTGGCCACCCCGAGCTTTTTCACCAGCTCATGCTCTGGAAAGGAGATCTGAAGGGTG
TTCTCCAGGCTGCAGCAGAGAGAGGCGAGCTGACAGACAGTCTCGTGGCTGTGGCACCAGTGGCTGGCTA
CAGTGTATGGCTGTGGGCTGTGGAAGCCTTTGCCAAGCAGCTGTGTTTCCAGGACCAGTACGTCAAGGCT
GCCTCTTATCTGCTATCCATCCACAAAGTGTACGAAGCTGTGGAACTTCTCAAGTCAAACCATCTCTACA
GAGAAGCTATTGCAGTTGCCAAGGCTCGGCTGCGCCCTGAGGACCCTGTTCTGAAGGAACTATACCTCAG
CTGGGGGTCCATCCTGGAGAGAGATGGCCACTATGCTATTGCAGCCAAGTGCTACTTAGGAGCCACTTCC
GCCTATGATGCAGCCAAAGTTCTGGCCAGAAAGGGAGATGCAGCCTCACTTAGAACAGCTGCAGAGCTGG
CTGCCATAGCAGGAGAACATGAGCTGGCTGCTTCCCTGGCTCTTAGATGTGCCCAAGAGCTGCTTCTGAT
GAAGAACTGGGTGGGTGCACAAGAAGCATTGGGACTGCATGAAAGCCTGCAGGGCCAGAGACTGGTCTTC
TGCCTCCTTGAACTTCTATGCCGGCACCTGGAGGAAAAACAGCCTCTAGAGGTCAGAGGCCCCTCTTCCA
TTTACCACCAGTGGGCCACAGGTTCTGAGGGAACCTTGGTGCAGAGAGTAACCGGTGTGTGGCGCAGCGC
CTTCAGTGTGGACACCCCGGAGCAGTGTCAGGCCGCCTTACAGAAGCTGCAGGATGTCAAGTACCCATCA
GCAACAAGTAACACTCCCTTCAGACAGCTGCTGCTTCATGTCTGCCATGACCTGACCTTGGCAATGCTGA
GCCAGCAGGCTGCTGCCTGGGAGGAGGCAGTGCCTGCTCTGCTGCAGGCTGTGGTTCGGAGCTATACCTC
AGGGAACTTCACCCTCATGCAGGAAATCTACTCAGCCTTTCTCCCAGGTGGCTGTGACCACCTACGAGAC
AAACTGGGTGACCTCTCTCCTGCCATGGCAGCTTACAAAAGCTTAGAGGCCTTTTGTATTTATGGGCAAC
TGTATGAAGTCTGGTGGTCGCTCTGCGGGCCTGGCCCTGAGTCAAGCGTCTGGGTGTTGTCAGCTGAAAG
CACGGTCTCTGATAAACAGAGCAAGCCAGAGGACAGTGCCAGTGCTGAGGACATGGAACAGCCTCCAGGC
CCGGGCCCGAGGCTCAGTGCAGAGAGTGAGCGTTTGCTGAGTGCCTGCAAGGAGCTTTTCTCAGAACGGC
ATGCCAGCCTGCAGACCTCCCAGAGGACTGTAGCCGAAGTCCAAGAGACACTGGCAGAAATGATCCGCCA
GCACCAAAAGAGTCAGCTCTGCAAGGCCACAACAAATGGTCCCAGCAGAGATGAACCCAGCAGAGATGAA
CCCAGCCAGGAAGCAGAGCGGGCTCCTTCTCAGCCTCCGAGCCCAACCAGAGAAGAAAGAAATGCACCAG
TGTCTCTCCCTGAACTAACCAGAAGGCTCACAGAGGCAAATGAGAGGATAGCAGAGTTTCCAGAGAGTGT
AAAGGCCTGGCCCTTTCCAGATGTGCTGGAGTGCTGTCTCGTCTTGCTTCACATTGGGTCCCAGTGTCCT
GACGCTGTGGACCCTGAGATGCAGCAACAGGCCCAGGAGCTCCTTCACAAGTATGGGCACACTCGAGCTT
ACAGAAGACACTGCCAGAGTCGGCACACGTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_001166669
Insert Size 4512 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001166669.1, NP_001160141.1
RefSeq Size 6229 bp
RefSeq ORF 4512 bp
Locus ID 216766
UniProt ID Q8BX17
Cytogenetics 11 B1.3
Summary Required for the assembly of the SMN complex that plays a catalyst role in the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus. GEMIN5 acts as the snRNA-binding protein of the SMN complex. Binds to the 7-methylguanosine cap of RNA molecules (By similarity). Binds to the 3' UTR of SMN1 mRNA and regulates its translation; does not affect mRNA stability (PubMed:25911097). May play a role in the regulation of protein synthesis via its interaction with ribosomes (By similarity).UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).
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Documents
Resources
"NM_001166669" in other vectors (4)
SKU Description Size Price
MG215628 Gemin5 (tGFP-tagged) - Mouse gem (nuclear organelle) associated protein 5 (Gemin5) transcript variant 1, (10ug) 10 ug
$1,486.00
MR215628 Gemin5 (Myc-DDK-tagged) - Mouse gem (nuclear organelle) associated protein 5 (Gemin5), transcript variant 1 10 ug
$589.00 $1,286.00
MR215628L3 Lenti ORF clone of Gemin5 (Myc-DDK-tagged) - Mouse gem (nuclear organelle) associated protein 5 (Gemin5), transcript variant 1 10 ug
$1,586.00
MR215628L4 Lenti ORF clone of Gemin5 (mGFP-tagged) - Mouse gem (nuclear organelle) associated protein 5 (Gemin5), transcript variant 1 10 ug
$1,586.00

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