Arid1a (BC082554) Mouse Untagged Clone

CAT#: MC224596

Arid1a (untagged) - Mouse AT rich interactive domain 1A (Swi1 like) (cDNA clone MGC:96305 IMAGE:30622048), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Arid1a
• Synonyms: 1110030E03Rik; BAF250a; Osa1; Smarcf1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC082554
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCAGATGGGAAAGATGAGACCTCAGCCGTATGGTGGGACTAACCCATACTCGCAACAACAGGGAC
  CTCCTTCAGGACCGCAACAAGGACATGGGTACCCAGGGCAGCCATATGGGTCCCAGACTCCACAGCGGTA
  CCCCATGACCATGCAGGGCCGGGCTCAGAGTGCCATGGGCAGCCTCTCTTATGCACAGCAGATTCCACCT
  TATGGCCAGCAAGGCCCCAGTGCGTATGGCCAGCAGGGCCAGACTCCATACTATAACCAGCAAAGTCCTC
  ATCCCCAGCAGCAGCCACCTTACGCCCAGCAACCACCATCCCAGACCCCTCATGCCCAGCCTTCGTATCA
  GCAGCAGCCGCAGACTCAGCAACCACAGCTTCAGTCCTCTCAGCCTCCATATTCCCAGCAGCCATCCCAG
  CCTCCACATCAGCAGTCCCCAACTCCATATCCCTCCCAGCAGTCCACCACACAACAGCATCCCCAGAGCC
  AGCCCCCCTACTCACAACCACAGGCACAGTCTCCCTACCAGCAGCAGCAACCTCAGCAGCCAGCATCCTC
  GTCGCTCTCCCAGCAGGCTGCATATCCTCAGCCCCAGCCTCAGCAGTCCCAGCAAACTGCCTATTCCCAG
  CAGCGCTTCCCTCCACCACAGGAGCTTTCTCAAGATTCATTTGGGTCTCAGGCATCCTCAGCCCCCTCAA
  TGACCTCCAGTAAGGGAGGGCAAGAAGATATGAACCTGAGTCTTCAGTCAAGGCCCTCCAGCTTGCCTGA
  TCTGTCTGGTTCAATCGATGATCTCCCCATGGGGACAGAAGGAGCTCTGAGTCCTGGCGTGAGCACATCA
  GGGATTTCCAGCAGCCAAGGAGAGCAGAGCAATCCAGCTCAGTCTCCCTTTTCTCCTCACACCTCCCCTC
  ACCTGCCTGGCATCCGAGGCCCGTCCCCGTCCCCTGTTGGCTCTCCTGCCAGTGTCGCGCAGTCTCGCTC
  AGGACCACTCTCGCCTGCTGCAGTGCCAGGCAACCAGATGCCACCTCGGCCACCCAGTGGCCAGTCAGAC
  AGCATCATGCACCCTTCCATGAACCAATCAAGCATTGCCCAAGATCGAGGTTATATGCAGAGGAACCCCC
  AGATGCCCCAGTACACTTCCCCTCAGCCTGGCTCGGCCTTATCCCCACGTCAGCCGTCTGGAGGACAGAT
  GCACTCGGGCGTGGGCTCCTACCAGCAGAACTCCATGGGGAGCTACGGCCCCCAGGGCAGTCAGTATGGC
  CCACAAGGAGGCTATCCTAGGCAGCCTAACTATAATGCCTTGCCCAACGCCAACTACCCCAATGCAGGCA
  TGGCCGGAAGTATGAACCCTATGGGTGCTGGAGGTCAGATGCATGGGCAGCCTGGAATCCCACCTTACGG
  CACACTCCCTCCAGGGAGAATGGCTCATGCGTCTATGGGCAACAGGCCCTATGGCCCTAATATGGCCAAT
  ATGCCACCTCAGGTTGGGTCAGGGATGTGTCCTCCACCAGGGGGAATGAACAGGAAAACTCAAGAGTCTG
  CTGTTGCCATGCATGTTGCTGCCAACTCTATCCAAAACAGGCCACCAGGCTACCCAAATATGAATCAAGG
  GGGCATGATGGGAACTGGACCTCCCTATGGACAGGGGATCAATAGTATGGCTGGCATGATCAACCCTCAG
  GGACCCCCATATCCTATGGGTGGAACCATGGCCAACAATTCAGCAGGGATGGCAGCCAGCCCAGAGATGA
  TGGGCCTTGGGGATGTTAAGTTAACTCCCGCCACAAAAATGAACAACAAGGCAGATGGAACACCCAAGAC
  AGAATCCAAATCTAAGAAATCCAGTTCTTCTACCACCACCAATGAGAAGATCACCAAATTGTATGAGTTG
  GGTGGTGAGCCCGAGAGGAAGATGTGGGTGGACCGGTACCTGGCCTTCACAGAGGAGAAGGCCATGGGCA
  TGACAAATCTGCCTGCTGTGGGGAGGAAGCCTCTGGACCTCTATCGCCTCTATGTGTCTGTGAAGGAGAT
  TGGTGGGTTGACTCAGGTCAACAAGAACAAAAAATGGCGGGAACTTGCAACCAACCTCAATGTGGGTACA
  TCAAGCAGTGCTGCCAGCTCACTGAAAAAGCAGTATATCCAATGTCTCTATGCCTTTGAGTGCAAGATCG
  AGCGTGGAGAAGACCCTCCCCCCGATATCTTCGCAGCTGCTGACTCCAAGAAGTCCCAACCCAAGATCCA
  GCCCCCCTCTCCTGCGGGATCAGGGTCTATGCAGGGGCCACAAACTCCTCAGTCAACCAGCAGTTCTATG
  GCAGAAGGAGGAGACCTGAAGCCACCAACTCCAGCATCCACACCACATAGTCAAATTCCCCCCTTACCAG
  GCATGAGGAGCAACTCAGTCGGAATCCAGGATGCCTTTCCTGATGGAAGTGACCCCACATTCCAGAAGCG
  GAATTCCATGACTCCAAACCCTGGGTACCAGCCCAGTATGAATACCTCTGACATGATGGGGCGCATGTCC
  TATGAGCCAAATAAGGATCCTTATGGCAGCATGAGGAAAGCGCCAGGAAGTGATCCCTTCATGTCCTCAG
  GGCAGGGCCCCAATGGCGGGATGGGTGATCCCTACAGCCGTGCTGCTGGCCCTGGGCTGGGAAGTGTGGC
  GATGGGACCACGGCAGCACTATCCCTATGGAGGTCCTTACGACAGAGTGAGGACGGAGCCTGGAATCGGG
  CCTGAAGGAAATATGGGCACTGGAGCCCCTCAGCCAAATCTCATGCCTTCCACCCCAGATTCGGGGATGT
  ATTCTCCTAGCCGCTACCCCCCGCAGCAGCAGCAGCAACAGCAGCAACAGCAGCAACAGCAACGACATGA
  TTCCTATGGCAATCAATTCTCTACCCAAGGCACCCCTTCCAGCAGCCCCTTCCCCAGCCAGCAGACCACA
  ATGTATCAGCAGCAGCAGCAGAATTATAAGAGGCCAATGGATGGCACATATGGCCCCCCTGCCAAGCGGC
  ATGAAGGGGAGATGTACAGTGTGCCGTACAGCGCTGGGCAAGGCCAGCCTCAACAGCAGCAGTTGCCTGC
  AGCTCAGTCCCAGCCTGCCAGCCAGCCACAAGCTGCCCAGCCTTCCCCTCAGCAGGACGTGTACAACCAG
  TACAGCAATGCCTACCCTGCCTCCGCCACCGCTGCTACTGATCGCCGACCAGCAGGCGGCCCCCAGAACC
  AATTTCCATTCCAGTTTGGCCGAGACCGAGTCTCTGCACCTCCTGGTTCCAGTGCCCAGCAGAACATGCC
  ACCACAAATGATGGGTGGCCCCATACAGGCATCAGCTGAGGTTGCTCAGCAGGGCACCATGTGGCAGGGG
  CGAAATGACATGACCTACAATTATGCCAACAGGCAGAACACAGGCTCTGCCACCCAGGGCCCTGCGTATC
  ATGGTGTGAACCGAACAGATGAAATGCTCCACACAGATCAGAGGGCCAACCATGAAGGCCCATGGCCTTC
  CCATGGCACACGCCAGCCTCCGTATGGTCCTTCAGCCCCTGTTCCCCCCATGACAAGGCCCCCTCCATCT
  AACTACCAGCCCCCACCAAGCATGCCGAATCACATTCCTCAGGTATCCAGCCCCGCTCCCCTCCCCCGGC
  CCATGGAGAACCGTACTTCTCCTAGCAAGTCTCCATTCCTGCACTCTGGGATGAAAATGCAAAAGGCGGG
  TCCACCGGTGCCTGCTTCGCACATAGCGCCTACCCCTGTGCAGCCGCCTATGATTCGGCGGGATATCACC
  TTCCCACCTGGCTCTGTAGAGGCCACTCAGCCTGTGTTGAAGCAGAGAAGGCGGCTCACAATGAAAGACA
  TTGGAACCCCGGAGGCATGGCGGGTAATGATGTCCCTCAAGTCCGGGCTCCTGGCAGAGAGCACGTGGGC
  GTTAGACACCATTAACATTCTACTGTATGATGACAACAGCATTATGACCTTCAACCTCAGCCAGCTCCCA
  GGCTTGCTAGAGCTCCTTGTGGAATATTTCCGTAGATGCCTAATTGAAATCTTTGGCATTTTAAAGGAGT
  ATGAGGTAGGGGACCCAGGACAGAGAACATTACTAGACCCTGGGAGATTCACCAAGGTGTATAGTCCAGC
  CCATACAGAGGAAGAAGAGGAAGAACACCTTGATCCTAAACTGGAGGAGGAAGAGGAAGAAGGGGTTGGA
  AATGATGAGGAGATGGCCTTTTTGGGCAAGGACAAGCCATCTTCAGAGAATAATGAGGAGAAGCTAGTCA
  GTAAGTTTGACAAGCTTCCGGTAAAGATCGTGCAGAGGAATGACCCATTTGTGGTGGACTGCTCAGATAA
  GCTTGGGCGCGTGCAGGAGTTTGACAGTGGCCTGCTACACTGGCGGATTGGTGGTGGGGATACCACTGAG
  CATATCCAGACCCACTTTGAGAGCAAGATAGAGCTGCTGCCTTCCCGGCCTTATGTGCCCTGCCCAACGC
  CCCCTCGGAAACACCTCACAACAGTAGAGGGCACACCAGGGACAACGGAGCAGGAGGGCCCCCCGCCCGA
  TGGCCTTCCAGAGAAAAGGATCACAGCCACCATGGATGACATGTTGTCTACCCGGTCTAGCACATTGACT
  GATGAGGGGGCAAAGAGTGCAGAGGCCACCAAGGAAAGCAGCAAGTTTCCATTTGGCATTAGCCCAGCAC
  AGAGCCACCGGAACATCAAAATTTTAGAGGATGAACCCCATAGTAAGGATGAGACCCCACTGTGTACCCT
  TCTGGACTGGCAGGATTCCCTTGCTAAGCGCTGTGTCTGTGTCTCCAATACCATCCGGAGCCTGTCGTTT
  GTGCCAGGCAACGACTTTGAGATGTCCAAACACCCAGGGCTGCTGCTTATCCTGGGCAAGCTGATCCTGC
  TGCACCACAAGCACCCAGAGCGGAAGCAGGCACCACTAACTTATGAGAAGGAGGAGGAACAGGACCAAGG
  GGTGAGCTGTGACAAAGTGGAGTGGTGGTGGGACTGCTTGGAGATGCTCCGAGAAAACACGCTGGTCACC
  CTCGCCAACATCTCGGGGCAATTGGACCTATCCCCATATCCTGAGAGCATCTGCCTGCCTGTCCTGGACG
  GACTCCTACACTGGGCAGTTTGCCCTTCAGCTGAAGCCCAGGACCCCTTCTCAACCCTAGGCCCCAATGC
  CGTCCTCTCCCCCCAGAGATTGGTCTTGGAAACCCTCAGCAAACTCAGCATCCAGGACAACAATGTGGAC
  CTGATCCTGGCCACTCCCCCTTTTAGCCGCCTGGAGAAGTTGTATAGTACCATGGTGCGCTTCCTCAGTG
  ACCGAAAGAACCCAGTGTGCCGGGAGATGGCCGTGGTACTGCTGGCAAATCTGGCCCAGGGGGACAGCCT
  GGCAGCCCGGGCCATTGCAGTGCAGAAGGGCAGCATCGGCAACCTCCTGGGTTTCCTGGAGGACAGCCTT
  GCTGCCACACAGTTCCAGCAGAGCCAGGCAAGCCTCCTGCATATGCAGAATCCACCCTTTGAACCAACTA
  GTGTGGACATGATGCGGCGGGCTGCCCGAGCACTGCTTGCCCTGGCCAAGGTGGATGAGAACCACTCAGA
  GTTCACTCTGTATGAGTCACGGCTGTTGGACATCTCCGTGTCACCACTGATGAACTCATTGGTTTCACAA
  GTCATTTGTGATGTACTGTTTTTGATTGGCCAGTCATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC082554
• Insert Size: 5709 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC082554, AAH82554
• RefSeq Size: 6487 bp
• RefSeq ORF: 5708 bp
• Locus ID: 93760
• Cytogenetics: 4 D2.3
• Gene Summary: Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Binds DNA non-specifically (PubMed:22952240, PubMed:26601204). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (PubMed:17640523).[UniProtKB/Swiss-Prot Function]