Mink1 (BC052474) Mouse Untagged Clone

CAT#: MC224580

Mink1 (untagged) - Mouse misshapen-like kinase 1 (zebrafish) (cDNA clone MGC:63403 IMAGE:6833893), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mink1
• Synonyms: B55, MINK, RP23-122P1.6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC052474
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGACCCAGCCCCCGCCCGCAGCCTGGACGACATCGACCTGTCTGCCCTGCGGGACCCTGCAGGAA
  TCTTTGAGCTGGTGGAGGTGGTTGGCAATGGAACCTATGGACAGGTATACAAGGGGCGGCACGTCAAGAC
  TGGGCAGCTGGCTGCCATTAAGGTCATGGATGTCACAGAGGATGAGGAGGAAGAGATCAAACAGGAAATC
  AACATGTTAAAGAAGTACTCTCACCATCGCAATATTGCCACCTACTATGGGGCCTTTATCAAGAAGAGCC
  CTCCTGGGAACGATGACCAGCTCTGGCTGGTGATGGAGTTCTGCGGTGCTGGTTCAGTGACCGACCTGGT
  AAAGAACACAAAAGGGAACGCACTGAAGGAGGATTGCATTGCTTACATCTGCAGGGAGATTCTCAGGGGT
  CTTGCCCATCTCCATGCCCACAAGGTGATCCACAGAGATATCAAGGGACAAAATGTGCTGCTGACAGAGA
  ATGCTGAAGTCAAGCTAGTGGATTTTGGGGTGAGTGCTCAGCTGGACCGCACTGTGGGCAGGCGGAACAC
  TTTCATTGGAACCCCATACTGGATGGCTCCAGAAGTCATTGCCTGTGACGAGAACCCCGATGCCACCTAT
  GACTACAGGAGTGACATTTGGTCTCTAGGAATCACAGCCATTGAAATGGCAGAGGGAGCCCCCCCTCTGT
  GTGACATGCACCCTATGCGGGCCCTCTTCCTCATCCCTCGGAACCCTCCCCCCAGGCTCAAGTCAAAGAA
  ATGGTCTAAGAAGTTCACTGACTTCATCGACACGTGTCTCATCAAGACTTACCTGAGCCGCCCACCCACC
  GAACAGTTACTCAAATTCCCCTTCATCCGAGACCAGCCCACGGAGCGGCAGGTCCGCATCCAGCTCAAGG
  ACCACATCGACCGCTCGCGGAAGAAGCGGGAGGAGACAGAGTATGAGTACAGCGGCAGTGAGGAGGAAGA
  CGACAGCCATGGAGAGGAAGGCGAGCCAAGCTCCATCATGAATGTGCCCGGTGAGTCCACACTGCGCAGA
  GAATTCCTCAGACTCCAGCAGGAGAATAAGAGCAACTCTGAGGCTTTAAAGCAGCAGCAGCAGCTGCAGC
  AACAGCAGCAGCGGGACCCGGAGGCACACATCAAACACCTGCTGCACCAGCGGCAGCGTCGCATAGAGGA
  GCAGAAGGAGGAGCGGCGACGTGTGGAGGAGCAACAGCGGCGAGAGCGAGAACAGCGTAAGCTACAAGAG
  AAGGAGCAGCAGCGGCGATTGGAAGACATGCAAGCCCTACGACGAGAGGAAGAGAGGCGGCAAGCAGAGC
  GGGAACAGGAATACAAGCGGAAGCAGCTGGAGGAGCAGCGGCAGTCAGAGCGGCTGCAGAGACAGCTGCA
  GCAGGAGCACGCCTACCTCAAGTCCCTGCAGCAGCAGCAGCAGCAGCAGCAGCTCCAGAAGCAGCAGCAG
  CAGCAGCAGCAGATCCTGCCTGGAGACAGGAAGCCCCTGTATCATTACGGTCGGGGCATTAATCCTGCTG
  ACAAGCCAGCATGGGCCCGCGAGGTGGAAGAGAGAGCACGGATGAACAAGCAGCAGAACTCTCCCTTGGC
  GAAGGCGAAGCCAAGCAGTGCGGGGCCAGAGCCCCCCATCTCCCAGGCCTCTCCTAGCCCCCCAGGACCT
  CTTTCCCAGACTCCTCCTATGCAGAGGCCTGTGGAGCCCCAGGAAGGACCGCACAAGAGCCTGGTGGCAC
  ACCGGGTCCCACTGAAGCCATATGCAGCACCTGTACCCCGATCCCAGTCCCTGCAGGACCAGCCGACTCG
  AAACCTGGCTGCCTTCCCAGCCTCCCACGACCCTGACCCTGCTGCTGTCCCTACACCCACTGCCACACCC
  AGTGCCCGAGGAGCTGTCATCCGCCAGAATTCAGACCCCACCTCTGAAGGGCCAGGGCCTAGCCCAAACC
  CTCCATCCTGGGTTCGGCCTGATAATGAGGCTCCACCTAAGGTTCCACAGAGGACCTCTTCTATCGCCAC
  TGCCCTTAACACCAGTGGGGCCGGAGGGTCCCGGCCAGCTCAGGCTGTCCGTGCCAGACCTCGCAGTAAC
  TCCGCCTGGCAAATCTATCTGCAGAGGCGGGCAGAGCGGGGCACCCCCAAGCCTCCCGGGCCCCCAGCTC
  AGCCCCCTGGCCCGCCCAACGCCTCTAGTAACCCTGACCTCAGGAGGAGTGACCCTGGCTGGGAGCGCTC
  AGACAGTGTCCTCCCGGCCTCCCACGGCCACCTCCCTCAGGCTGGCTCCTTGGAGCGGAACCGAAACCGT
  GTGGGAGCCTCCACAAAACTGGATAGCTCTCCAGTGCTCTCCCCTGGGAACAAAGCCAAGCCTGAAGACC
  ACCGCTCAAGGCCAGGCCGGCCCGCAGACTTTGTGTTGCTCAAAGAGCGGACTCTGGATGAGGCCCCTAA
  GCCTCCCAAGAAGGCCATGGACTACTCCTCATCCAGTGAGGAGGTGGAAAGCAGTGAAGAGGAGGAGGAG
  GAAGGCGATGGGGAGCCGTCAGAGGGGAGCAGAGACACTCCCGGGGGCCGCAGTGATGGTGATACAGACA
  GCGTCAGCACCATGGTGGTTCATGATGTTGAGGAGATATCCGGGACCCAGCCCTCATATGGCGGCGGCAC
  CATGGTGGTCCAGCGTACTCCTGAAGAGGAACGAAGCCTGCTGCTTGCTGATAGCAATGGCTACACAAAC
  CTGCCTGATGTGGTCCAGCCCAGCCACTCACCTACTGAGAACAGCAAAGGTCAAAGCCCTCCAACAAAGG
  ATGGAGGCAGTGATTACCAGTCTCGTGGGCTGGTAAAGGCCCCAGGAAAGAGCTCATTCACCATGTTTGT
  GGATCTAGGGATCTACCAGCCTGGAGGCAGTGGGGACACCATCCCTATCACAGCCCTAGTGGGTGGAGAA
  GGTGGTCGCCTTGATCAACTGCAGTTCGATGTGAGGAAGGGCTCTGTGGTCAACGTCAATCCCACCAACA
  CCCGAGCTCATAGTGAAACTCCTGAAATTCGCAAGTACAAGAAGCGATTCAACTCAGAGATCCTATGTGC
  AGCTCTCTGGGGGGTCAACCTCCTAGTGGGCACAGAGAATGGGCTGATGTTGCTGGACCGAAGTGGGCAG
  GGCAAGGTGTATGGACTTATTGGGCGACGACGCTTCCAGCAAATGGATGTCTTAGAAGGGCTCAACTTGC
  TCATCACCATCTCAGGGAAAAGGAACAAACTGCGGGTATATTACCTGTCCTGGCTTCGGAACAAGATCCT
  ACACAATGACCCAGAGGTGGAAAAGAAGCAGGGGTGGACCACCGTGGGGGACATGGAGGGCTGCGGCCAC
  TACCGTGTTGTGAAATATGAACGGATTAAGTTCCTGGTCATTGCCCTGAAGAACTACGTGGAGGTTTATG
  CCTGGGCTCCCAAACCCTACCACAAATTCATGGCCTTCAAGTCCTTTGCTGACCTCCCTCACCGCCCTCT
  ACTGGTGGACCTGACAGTAGAGGAGGGACAGCGGCTCAAGGTCATCTATGGCTCCAGTGCTGGCTTCCAT
  GCTGTGGATGTTGATTCTGGGAACAGCTATGACATCTACATCCCTGTACATATCCAGAGCCAGATCACAC
  CCCACGCCATCATCTTCCTCCCCAACACTGATGGCATGGAGATGCTGCTGTGCTATGAAGATGAGGGTGT
  CTATGTCAACACTTACGGGCGGATCATCAAGGATGTGGTGCTGCAGTGGGGAGAGATGCCCACCTCTGTG
  GCCTACATCTGCTCCAACCAGATAATGGGCTGGGGTGAGAAGGCCATAGAGATCCGCTCTGTGGAGACAG
  GCCACCTAGATGGGGTCTTCATGCACAAACGAGCCCAGAGGCTCAAGTTCCTGTGTGAGCGCAATGACAA
  GGTGTTTTTTGCCTCTGTCCGCTCTGGAGGAAGCAGCCAAGTTTACTTTATGACTCTGAACCGTAACTGC
  ATCATGAACTGGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC052474
• Insert Size: 4005 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC052474, AAH52474
• RefSeq Size: 4842 bp
• RefSeq ORF: 4004 bp
• Locus ID: 50932
• Cytogenetics: 11 B3
• Gene Summary: Serine/threonine kinase which acts as a negative regulator of Ras-related Rap2-mediated signal transduction to control neuronal structure and AMPA receptor trafficking. Required for normal synaptic density, dendrite complexity, as well as surface AMPA receptor expression in hippocampal neurons. Can activate the JNK and MAPK14/p38 pathways and mediates stimulation of the stress-activated protein kinase MAPK14/p38 MAPK downstream of the Raf/ERK pathway. Phosphorylates: TANC1 upon stimulation by RAP2A, MBP and SMAD1. Has an essential function in negative selection of thymocytes, perhaps by coupling NCK1 to activation of JNK1.[UniProtKB/Swiss-Prot Function]