Prx (BC068135) Mouse Untagged Clone

CAT#: MC224571

Prx (untagged) - Mouse periaxin (cDNA clone MGC:92965 IMAGE:30354483), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Prx
• Synonyms: L-Periaxin
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC068135
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGACCCCTTGCTGCCCCTCGCAGGAGCTGAGACGGGCGGAGTTGGTGGAGATTATCGTGGAGACCG
  AGGCACAGACCGGGGTCAGCGGCTTCAACGTAGCAGGCGGCGGCAAAGAAGGAATCTTTGTCCGTGAGCT
  GCGAGAGGACTCACCGGCAGCTAAGAGCCTCAGCTTGCAAGAAGGGGACCAGCTGCTGAGTGCCCGTGTG
  TTCTTTGAGAACTTCAAATATGAGGATGCACTTCGCCTGCTGCAATGCGCAGAGCCCTACAAGGTCTCCT
  TCTGCTTGAAGCGCACTGTGCCCACCGGGGATCTGGCACTGAGGCCCGGGACGGTGTCTGGATACGAGAT
  GAAGGGCCCACGGGCCAAAGTGGCCAAGCTGAACATCCAGAGTCTGGCCCCTGTGAAGAAGAAGAAGATG
  GTGACTGGGGCCCTGGGGACCCCTGCAGATTTGGCCCCTGTTGACGTCGAGTTCTCTTTTCCCAAGTTCT
  CCCGACTGCGTCGGGGTCTCAAAGCCGAGGCTGTCAAGGGACCTGTCCCAGCTGCCCCAGCCCGTCGCCG
  CCTCCAGCTGCCTCGGCTGCGTGTCCGAGAAGTAGCTGAAGAGGCCCAGGTAGCCCGAATGGCTGCTGCT
  GCTCCTCCCCCAAGGAAGGCCAAGGCAGAAGCTGAGGCAGCCACAGGAGCTGGGTTCACAGCCCCTCAGT
  TAGAGCTAGTTGGGCCTCGGCTGCCTAGTGCCGAGGTGGGTGTCCCTCAGGTCTCAGTTCCCAAGGGGAC
  CCCATCAACAGAGGCAGCCAGCGGCTTTGCCCTTCACCTGCCAACCCTTGGGCTAGGTGCCCCAGCTGCA
  CCGGCTGTGGAGCCCCCAGCCACGGGAATCCAGGTTCCACAAGTGGAACTCCCCACCCTGCCCTCTCTAC
  CCACTCTTCCCACACTTCCATGCCTGGACACCCAGGAAGGAGCTGCAGTGGTAAAAGTCCCTACCCTGGA
  TGTGGCAGCTCCGTCTATGGGGGTGGACCTGGCTTTGCCGGGTGCAGAGGTGGAGGCCCAGGGAGAGGTT
  CCTGAAGTGGCCCTCAAGATGCCCCGGCTCAGTTTCCCCCGTTTTGGGATTCGGGGGAAGGAAGCCACTG
  AAGCCAAAGTAGTCAAGGGCAGCCCTGAGGCCAAAGCAAAGGGTCCCAGACTTCGAATGCCCACCTTTGG
  GCTTTCTCTCCTGGAACCCCGGCCCTCTGGCCCTGAAGCTGTTGCTGAGAGCAAGCTGAAGCTACCCACC
  CTCAAGATGCCCTCTTTCGGCATTGGTGTGACTGGGCCTGAGGTCAAGGCACCCAAGGGGCCCGAAGTAA
  AGCTCCCTAAGGTTCCTGAGGTCAAACTCCCGAAAGTGCCCGAGGCAGCCATTCCAGATGTGCAACTCCC
  TGAGGTACAGCTGCCCAAAATGTCAGACATGAAACTTCCAAAGATCCCTGAGATGGTTGTACCCGACGTT
  CGTCTTCCGGAAGTGCAGCTGCCCAAAGTCCCTGAGATGAAAGTCCCAGAGATGAAGCTCCCGAAGGTGC
  CCGAGATGGCCGTGCCCGATGTACACCTTCCAGATGTACAGCTCCCGAAAGTCCCAGAGATGAAGCTCCC
  GAAGGTGCCCGAGATGGCCGTGCCCGATGTACACCTTCCAGATGTACAGCTCCCGAAAGTTCCAGAGATG
  AAGCTACCAGAGATGAAGCTCCCGAAGGTGCCGGAGATGGCCGTGCCGGATGTACGACTCCCGGAAGTTC
  AGCTGCCCAAAGTGTCTGAGGTGAAGCTCCCAAAGATGCCTGAGATGGCCGTGCCTGATGTCCACCTCCC
  GGAGCTACAACTTCCCAAAATGTCCGAGGTGAAGCTCCCAAAGATGCCCGAGATGGCCGTGCCCGATGTT
  CGCCTCCCGGAAGTTCAGCTGCCCAAAGTGTCAGAGATGAAACTCCCTAAGATGCCAGAGATGACCATGC
  CCGACATTCGCCTCCCAGAAGTTCAGTTGCCCAAAGTGCCTGACATTAAACTTCCTGAAATGAAGCTTCC
  AGAAATAAAACTCCCCAAAGTGCCTGACATGGCAGTGCCTGATGTCCCCCTTCCAGAGCTGCAGCTGCCC
  AAAGTGTCGGACATTCGGCTGCCTGAAATGCAAGTGTCACAGGTCCCAGAGGTGCAGCTTCCCAAGATGC
  CAGAGATGAAGTTGTCCAAGGTTCCTGAGGTACAGAGGAAATCTGCAGGGGCGGAGCAGGCAAAAGGGAC
  TGAATTTAGTTTCAAGTTGCCCAAGATGACCATGCCCAAGTTGGGGAAAGTGGGCAAGCCTGGGGAGGCA
  AGTATTGAGGTTCCAGACAAACTCATGACACTTCCCTGTCTGCAGCCAGAGGTGGGCACTGAGGCATCCC
  ATGTTGGTGTCCCTTCCCTCTCTCTCCCCTCTGTGGAGCTTGACTTGCCTGGGGCCCTGGGCCTGGAGGG
  ACAAGTCCAAGAAGCTGTCCCAGGCAAAGTGGAGAAGCCAGAGGGCCCCAGGGTAGCAGTGGGTGTTGGA
  GAGGTGGGCTTTCGTGTGCCCTCTGTGGAGATTGTCACTCCTCAGCTGCCCACAGTTGAAGTTGAGAAAG
  AGCAGCTAGAGATGGTGGAGATGAAAGTCAAACCCTCTTCCAAGTTCTCTCTGCCCAAATTCGGACTTTC
  AGGGCCCAAAGCTGTCAAGGGAGAGGTGGAGGGGCCTGGGCGAGCCACCAAGCTGAAGGTTTCCAAGTTT
  ACCATCTCACTTCCCAAAGCTCGAGCAGGGACTGAGGCCGAAGCGAAGGGAGCTGGGGAAGCCGGGTTGC
  TGCCAGCGCTGGATCTGTCCATCCCACAGCTCAGCCTGGATGCCCAGCTGCCCTCAGGCAAGGTGGAAGT
  AGCTGATAGCAAGCCTAAATCGTCCAGATTTGCTCTGCCCAAGTTTGGGGTGAAAGGCCGGGACTCTGAG
  GCTGATGTACTGGTGGCAGGGGAGGCTGAGCTTGAGGGAAAGGGTTGGGGCTGGGATGGGAAGGTGAAGA
  TGCCCAAGCTGAAAATGCCATCTTTTGGGTTGTCCCGAGGAAAGGAAGCAGAAACTCAGGATGGACGTGT
  CAGCCCCGGGGAAAAGCTGGAGGCCATAGCTGGGCAGCTTAAGATCCCTGCGGTGGAATTGGTCACACCG
  GGAGCTCAGGAGACAGAGAAGGTCACCAGTGGAGTGAAGCCGTCAGGCCTCCAGGTGTCCACCACTGGGC
  AGGTGGTTGCAGAGGGCCAGGAGAGTGTGCAGAGGGTGTCCACACTAGGTATCTCTTTGCCCCAGGTGGA
  ATTGGCCAGCTTTGGGGAGGCAGGCCCTGAGATCGTAGCCCCTTCTGCAGAGGGCACAGCAGGCTCTAGG
  GTCCAGGTGCCACAGGTGATGCTGGAGCTACCTGGAACCCAGGTGGCAGGGGGTGATCTGTTAGTGGGTG
  AGGGCATCTTCAAGATGCCCACAGTGACAGTGCCCCAGCTAGAGCTGGATGTGGGGCTGGGCCATGAAGC
  CCAGGCTGGTGAAGCAGCCAAGAGTGAGGGTGGGATAAAGTTGAAGTTGCCCACACTGGGGACCGGAAGC
  AGAGGAGAGGGCGTTGAGCCCCAGGGCCCCGAGGCCCAGCGCACCTTCCACCTCTCATTGCCCGATGTGG
  AACTCACGTCACCAGTGAGTAGCCACGCTGAGTACCAGGTAGTTGAGGGTGATGGGGATGGTGGGCACAA
  ACTCAAGGTTCGGCTGCCCCTGTTTGGTCTGGCAAAGGCCAAGGAAGGGATAGAAGTTGGAGAAAAGGCT
  AAGAGTCCAAAGCTCAGGCTACCCCGAGTGGGCTTCAGCCAGAGTGAGTCGGTCTCCGGAGAAGGCTCTC
  CAAGTCCTGAGGAGGAGGAAGAAGGCAGTGGGGAAGGGGCTTCCAGTCGCCGGGGTAGGGTAAGGGTCCG
  CCTGCCTCGGGTAGGCTTGGCTTCCCCTTCTAAAGTCTCTAAGGGACAGGAGGGTGATGCAACCTCCAAG
  TCCCCAGTTGGGGAGAAGTCACCCAAATTCCGTTTTCCTAGGGTGTCCTTAAGCCCCAAGGCCCGGAGTG
  GGAGTAGGGACCGGGAAGAAGGTGGATTCAGGGTCCGACTGCCCAGTGTGGGATTTTCAGAAACAGCAGT
  TCCAGGTTCCACCAGGATTGAGGGAACCCAGGCTGCTGCCATCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC068135
• Insert Size: 4176 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC068135, AAH68135
• RefSeq Size: 4502 bp
• RefSeq ORF: 4175 bp
• Locus ID: 19153
• Cytogenetics: 7 15.91 cM
• Gene Summary: Scaffolding protein that functions as part of a dystroglycan complex in Schwann cells, and as part of EZR and AHNAK-containing complexes in eye lens fiber cells (PubMed:11430802, PubMed:21745462, PubMed:22764250). Required for the maintenance of the peripheral myelin sheath that is essential for normal transmission of nerve impulses and normal perception of sensory stimuli (PubMed:10839370). Required for normal transport of MBP mRNA from the perinuclear to the paranodal regions (PubMed:15356632). Required for normal remyelination after nerve injury (PubMed:10839370). Required for normal elongation of Schwann cells and normal length of the internodes between the nodes of Ranvier. The demyelinated nodes of Ranvier permit saltatory transmission of nerve impulses; shorter internodes cause slower transmission of nerve impulses (PubMed:15356632, PubMed:23022068). Required for the formation of appositions between the abaxonal surface of the myelin sheath and the Schwann cell plasma membrane; the Schwann cell cytoplasm is restricted to regions between these appositions (PubMed:15356632, PubMed:23022068). Required for the formation of Cajal bands and of Schmidt-Lanterman incisures that correspond to short, cytoplasm-filled regions on myelinated nerves (PubMed:23022068, PubMed:22764250). Recruits DRP2 to the Schwann cell plasma membrane (PubMed:11430802, PubMed:23022068, PubMed:22764250). Required for normal protein composition of the eye lens fiber cell plasma membrane and normal eye lens fiber cell morphology (PubMed:21745462).[UniProtKB/Swiss-Prot Function]