Ercc6 (NM_001081221) Mouse Untagged Clone

CAT#: MC224549

Ercc6 (untagged) - Mouse excision repair cross-complementing rodent repair deficiency, complementation group 6 (Ercc6), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ercc6
• Synonyms: 4732403I04; C130058G22Rik; CSB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224549 representing NM_001081221
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCCACGAGGAAGTTCCCAACTCCACTCACCCTCAGGAGCAAGACTGTTTACCAAGTCAGCATGCCA
  ATGCTTACAAAGACATGCCAGTTGGGCAGGAGAATGGTGGGGTCTCAGAGGCCGGGGAGTGCCTCTCCTC
  TACTTCCTGTGAGTATGGGCCATCCACATCTGCTGAAGCTTGTGTGTTGGCAGCCACAAGGCGTGGGCCG
  ACCCTGCTACACATTGATCGGCATCAGATCCCTGCAGTTGAGCCCAGTGCTCAGGCCCTGGAGCTGCAGG
  GCCTGGGTGTGGACGTCTATGACCAGGCTGTCCTGGAACAAGGCGTGCTTCAGCAGGTGGACAGTGCGAT
  GCATGAGGCCAGCTGTGTGGCCCAGCTTGCTGATGCGGAGAAGGAGTATCAGTCAGTCCTGGATGACCTC
  ATGTCATGTACAACATCCCTGAGGCAAATCAATAAAATTATAGAGCAACTTAGCCCTCAAGCTGCCAGCA
  ACAGAGACATCAACAGGAAACTAGATTCCGTGAAGCGACAGAAGTATAACAAGGAACAACAGCTAAAGAA
  GATAACTGCAAAACAGAAACGTCTCCAGGCCATTCTCGGAGGGGCTGGGGTCCAAGTGGAACTGGATCAT
  GCCAGCCTAGAGGAGGACGATGCAGAGCCAGGGCCATCATGTCTTGGCAGTATGCTCATGCCTGCCCAGG
  AGACCGCCTGGGAGGAGCTTATCCGCACTGGCCAGATGACACCGTTTGGTACCCCAGCCCCTCAGAAACA
  GGAGAAAAAGCCCAGAAAAATAATGCTGAATGAAGCATCAGGCTTTGAAAAATATTTGGCAGAGCAAGCA
  CAACTTTCGTTTGAAAGGAAGAAACAAGCTGCTACTAAAAGAACAGCTAAAAAAGCTATAGTCATATCCG
  AGTCAAGCCGAGCAGCAATTGAAACCAAGGCAGACCAGAGAAGCCAAGTGCTCTCTCAGACAGACAAGCG
  CCTGAAGAAGCACAGCAGGAAACTCCAGAGGAGGGCGCTGCAGTTCCAGGGCAAAGTGGGATTGCCGAGC
  GGGAAGAAACCTTTGGAGCCTGAGGTGAGGCCAGAGGCCGAGGGAGACACTGAGGGTGAGGAGTCTGGGT
  CCTCTCCCACCGATGGGGAGGAGGAGGAAGAACAAGAGGAGGAGGAAGGGGTAGCCAGCCTGTCTTCTGA
  TGACGTTAGCTATGAGCTGAAGCCTCTTCGGAAGAGGCAGAAATACCAGAAGAAAGTCCCAGTTCAGGAG
  ATCGACGATGACTTCTTTCCAAGTTCTGAGGAGGAAGATGAAGCTATGGAAGGAAGAGGAGGAGGCCGAA
  AGGTAGCCAGGCGCCAAGACGATGGAGATGAGGATTATTATAAGCAGCGGTTAAGGAGGTGGAACAGGCT
  GAGGCTGCAGGACAAAGAGAAACGCCTGAAGCTTGAAGATGATTCTGAGGAGAGCGATGCCGAATTTGAC
  GAAGGTTTCAAAGTGCCGGGTTTTCTGTTCAAAAAGCTCTTTAAGTATCAGCAGACAGGTGTTAGGTGGC
  TGTGGGAATTGCACTGCCAGCAGGCAGGAGGAATTCTGGGAGACGAAATGGGATTGGGCAAGACCATCCA
  GATAATTGCCTTCTTGGCAGGTCTGAGCTACAGCAAGATCAGGACTCGCGGTTCAAATTACAGGTTCGAG
  GGACTAGGCCCCACTATAATTGTCTGCCCAACGACAGTGATGCATCAGTGGGTGAAGGAATTCCACACGT
  GGTGGCCTCCATTCAGAGTGGCAGTTCTGCATGAAACAGGTTCCTACACCCACAAAAAGGAGAGGCTGAT
  TCGAGATATTGTTTATTGTCATGGAGTTTTGATAACTTCATATTCATACATCCGACTGATGCAAGATGAC
  ATTAGCAGACATGACTGGCACTACGTCATCTTAGATGAAGGACACAAAATTCGAAATCCAAATGCTGCAG
  TCACCCTTGCTTGCAAACAGTTCCGGACACCTCATCGGATTATCTTGTCTGGCTCACCGATGCAGAATAA
  CCTCCGAGAGCTGTGGTCACTCTTCGACTTCATCTTCCCAGGAAAGCTGGGCACATTGCCTGTGTTTATG
  GAGCAGTTCTCTGTCCCCATCACCATGGGAGGCTATTCCAATGCTTCCCCAGTACAGGTTAAAACTGCTT
  ATAAGTGTGCATGTGTCTTAAGAGACACGATAAACCCGTACTTACTGCGGAGAATGAAATCAGATGTCAA
  GATGAGCCTTTCGTTGCCAGATAAAAATGAGCAGGTCTTATTTTGCCGTCTAACTGATGAGCAGCATAAA
  GTCTACCAGAATTTCATTGATTCTAAAGCAGTCTACAGGATTCTCAATGGAGAAAATCAGATTTTCTCTG
  GACTTGTAGCGCTGAGGAAGATCTGCAACCACCCTGACCTCTTTTCTGGCGGCCCCAAGAACGCCAGTGG
  TCCTCCAGAGGATGAGCTGGAAGAAGAGCAGTTTGGACACTGGAGACGCTCTGGGAAAATGATTGTTGTT
  GAGTCTTTGCTGAAAATATGGCACAGGCAGGGTCAGCGGGTGCTGCTGTTTTCCCAGTCCAGGCAGATGC
  TACACATACTGGAAGTGTTCCTGAGAGCCCATAAGTATTCCTATCTCAAGATGGATGGGACCACCACCAT
  AGCGTCAAGACAGCCGCTGATTACAAAATACAATGAGGACACGTCCATCTTTGTCTTTCTTCTGACCACA
  CGGGTGGGTGGCTTAGGAGTGAACCTGACCGGTGCCAACAGAGTCATCATCTACGACCCAGACTGGAATC
  CAAGCACCGACACACAGGCCCGGGAGCGAGCATGGAGGATAGGTCAGAAGAAGCAGGTGACTGTCTACAG
  GCTTCTGACGGCTGGCACCATTGAGGAAAAGATCTACCACCGACAAATCTTCAAGCAATTTTTGACAAAC
  AGAGTGCTAAAAGACCCAAAACAAAGGCGCTTTTTCAAATCCAACGATCTCTATGAGCTGTTCACTCTGA
  CCAGTCCTGACGCCTCCCAGGGCACTGAGACAAGTGCTATTTTTGCAGGAACGGGCTCCAGTATTCAGAC
  ACCCAAATGCCAGTTAAAAAAAAGGACTTCCACAGTCCTGGGAACAGATCCCAAATGCAAGAAGCCCCCT
  GTTTCTGACACACCTGCAAATGCTGCCACCCTGATTGGAGAAAAACCTAAGGCGGCGGGAGCTACAGGAA
  GAAGTGTCACTTCTGGAGAAAGTGGTCCTTTCAAAGGCGACCATGACACAAATGGGAACAGAGCTAGCAG
  TGTTGCTTTTGGAGAAGAGACAGATGCAGGGTCCACACTGGAGCATCTCTCAGTGATGAGTGGAGATGGG
  AAGCATTCAGATTCTCCAACAGTTGACCACACATCCAGGCCACCAGTGGAGGCAAGCACCAGTGAGAAGC
  AGGGTTCTTCCTATGCAGGGGCGCGCTGCCAGGCTCAGACAGAACCTGTTCCCATGAGTGAACAAATGGA
  AGGTCAGTTTTCTAAGTACAAATCCAAAAGGAAGCATGATGCATCTGAGGAAGAGACCACAGAGAAACGT
  CCACAGCCCAAGCAAAAGGCCAAGAACTCCAAGCATTGCAGAGATGCTAAGTTTGAGGGAACTCGAGTCC
  CGCACCTGGTGAAGAAAAGACGGTACCGCCAGCAAACCAGTGAGCAGGAGGGCGGAGCCAAGGACCGGAG
  CAGCGATGATTATGTGTTGGAAAAGCTTTTCAAAAAATCAGTGGGCGTGCACAGCGTCGTGAGGCATGAT
  GCCATCATAGATGGGTCCAGTCCAGATTATGTGCTGGTGGAAGCAGAAGCCAACCGAGTGGCTCAGGACG
  CCTTGAAAGCTCTGAGGCTCTCTCGTCAGCAATGCCTGGGGGCAGCATCTGGTGTTCCCACCTGGACTGG
  CCACAGGGGGATTTCTGGTGCACCAACAGGAGTAAAGAATAGGTTCGGTCAGAAAAGGGACTCCAGCCTC
  CCTGTGCAGCATCCTTCATCACTAACAGAAAAGACTCAGAACAACATGAAAAAGGAGGGAAAAGCTCACA
  CCCCTGAGCACTTCAGTGGCAAAGAAGATGGAGCATCTGTGTCTGGAGCGCCCAGTTCCTCCTCCCTCTT
  GGCCAGAATGCGAGCAAGAAACCACATGATTTTGCCGGAGCGCTTAGAGAGTGACAGTGAACATCTTGCT
  GAGGCTGCTGCCGTGCCTCCCTGCGGCACCGAACACGACGACCTTCTGGTGGACATGAGGAACTTCATTG
  CTTTCCAGGCCCAGGTGGATGGCCAGGCCAGCACTCAGGAAATCCTGCAGGAGTTTGAGTCCAAGCTGTC
  CGTGGCACAGTCCTGTGTCTTCCGAGAACTGCTGAGAAATCTGTGCAATTTCCATAGAACTCCTGGTGGG
  GAAGGGATTTGGAAGCTGAAGCCAGAATACTGCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001081221
• Insert Size: 4446 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081221.1, NP_001074690.1
• RefSeq Size: 8422 bp
• RefSeq ORF: 4446 bp
• Locus ID: 319955
• UniProt ID: F8VPZ5
• Cytogenetics: 14 B
• Gene Summary: Essential factor involved in transcription-coupled nucleotide excision repair which allows RNA polymerase II-blocking lesions to be rapidly removed from the transcribed strand of active genes (By similarity). Upon DNA-binding, it locally modifies DNA conformation by wrapping the DNA around itself, thereby modifying the interface between stalled RNA polymerase II and DNA (By similarity). It is required for transcription-coupled repair complex formation. It recruits the CSA complex (DCX(ERCC8) complex), nucleotide excision repair proteins and EP300 to the sites of RNA polymerase II-blocking lesions (By similarity). Plays an important role in regulating the choice of the DNA double-strand breaks (DSBs) repair pathway and G2/M checkpoint activation; DNA-dependent ATPase activity is essential for this function (By similarity). Regulates the DNA repair pathway choice by inhibiting non-homologous end joining (NHEJ), thereby promoting the homologous recombination (HR)-mediated repair of DSBs during the S/G2 phases of the cell cycle (By similarity). Mediates the activation of the ATM- and CHEK2-dependent DNA damage responses thus preventing the premature exit from the G2/M checkpoint (By similarity). Acts as a chromatin remodeler at DSBs; DNA-dependent ATPase-dependent activity is essential for this function (By similarity). Remodels chromatin by evicting histones from chromatin flanking DSBs, limiting RIF1 accumulation at DSBs thereby promoting BRCA1-mediated HR (By similarity). Required for stable recruitment of ELOA and CUL5 to DNA damage sites (By similarity). Involved in UV-induced translocation of ERCC8 to the nuclear matrix (By similarity). Essential for neuronal differentiation and neuritogenesis; regulates transcription and chromatin remodeling activities required during neurogenesis (By similarity).[UniProtKB/Swiss-Prot Function]