Magi3 (NM_001159354) Mouse Untagged Clone

CAT#: MC224534

Magi3 (untagged) - Mouse membrane associated guanylate kinase, WW and PDZ domain containing 3 (Magi3), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Magi3
• Synonyms: 4732496O19Rik; 6530407C02Rik; AA407180; AI120132; mKIAA1634
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224534 representing NM_001159354
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGAAGACGTTGAAGAAGAAGAAGCACTGGCTCAGCAAGGTGCAGGAGTGTGCGGTATCCTGGGCCG
  GGCCCCCGGGCGACTTGGGCGCCGAGATCCGCGGCGGCGCCGAGCGCGGCGAGTTCCCTTACCTGGGGCG
  GCTCCGCGACGAGGCTGGCGGCGGCGGAGGCACCTGCTGCGTGGTCTCGGGCAAGGCGCCCAGTCCGGGC
  GATGTGCTGTTGGAGGTGAACGGGACGCCGGTCAGTGGGCTCACCAACCGGGACACTCTGGCTGTCATCC
  GCCACTTCCGCGAGCCCATCCGTCTGAAGACGGTGAAGCCAGGCAAAGTTATAAATAAAGATTTGCGACA
  CTACCTGAGTCTTCAGTTTCAGAAAGGATCAATTGACCACAAACTACAGCAAGTCATCAGAGATAATCTC
  TATTTGAGAACCATCCCATGCACTACAAGGGCTCCCAGGGATGGGGAAGTCCCAGGGGTGGATTATAACT
  TCATTTCTGTTGAACAATTCAAGGCACTGGAAGAAAGCGGAGCATTGTTAGAAAGTGGAACGTATGATGG
  AAATTTCTATGGCACACCCAAGCCTCCAGCAGAGCCTAGTCCTTTCCAGCCCGATCCAGTTGATCAGGTC
  CTCTTTGACAATGAGTTTGACACAGAATCCCAAAGAAAAAGAACCACATCTGTCAGCAAGATGGAAAGGA
  TGGACAGCTCTCTCCCTGAAGAAGAAGAAGATGAGGACAAAGAAGCTGTTAATGGCAGCGGAAGCATGGA
  AACTAGAGAGATGCATTCTGAGACATCTGACTGCTGGATGAAGACTGTTCCAAGTTATAACCAAACAAAT
  AGCTCCATGGACTTTAGAAATTATATGATGAGAGATGAGAATCTGGAACCGCTGCCCAAAAACTGGGAAA
  TGGCCTACACTGACACAGGAATGATCTACTTCATTGATCATAATACGAAGACAACCACCTGGTTGGATCC
  TCGTCTCTGCAAGAAAGCCAAAGCCCCTGAAGACTGTGAAGATGGAGAGCTTCCCTATGGCTGGGAGAAA
  ATAGAGGACCCTCAGTATGGAACATACTACGTTGATCACCTCAACCAGAAAACTCAGTTTGAAAATCCAG
  TGGAGGAAGCCAAGAGGAAAAAGCAGTTAGGACAGGCTGAAATTCATTCTGCAAAAACAGATGTGGAAAG
  AGCACACTTTACTCGGGACCCATCCCAACTTAAAGGTGTACTTGTTCGAGCATCGCTGAAAAAAAGCACC
  ATGGGCTTTGGCTTTACCATTATTGGTGGAGATAGGCCTGATGAATTTCTACAAGTAAAAAACGTGCTCA
  AAGATGGTCCTGCCGCTCAGGATGGGAAAATTGCACCAGGTGATGTTATTGTAGACATCAATGGCAACTG
  TGTCCTTGGTCACACCCATGCAGATGTTGTCCAGATGTTTCAACTGGTACCTGTCAACCAGTATGTCAAC
  CTTACTTTATGCCGTGGTTATCCACTTCCTGATGACAGTGAAGATCCTGTTGTGGACATTGTTGCTGCTA
  CCCCTGTCATCAATGGACAATCTTTAACAAAGGGAGAGACATGCATGAATACTCAGGATTTTAAACTGGG
  AGCAATGGTTTTGGATCAGAATGGAAAATCAGGACAAATCTTGGCCAGTGATCGTCTCAATGGTCCATCT
  GAGTCAAGTGAGCAGAGGGCATCTCTGGCATCATCAGGCAGCTCACAGCCTGAACTAGTGACTATCCCTC
  TGATTAAGGGCCCCAAAGGCTTTGGGTTTGCAATTGCTGACAGCCCAACTGGACAGAAGGTGAAAATGAT
  ATTGGATAGCCAGTGGTGTCAAGGCCTTCAAAAAGGGGATATCATCAAAGAAATTTACCATCAGAATGTG
  CAGAATTTAACACATCTCCAAGTCGTAGAAGTCCTAAAGCAGTTCCCAGTAGGTGCAGATGTTCCATTGC
  TTATCTTAAGAGGAGGTCCTTGTTCACCAACTAAAACTGCCAAAACGAAAACAGATACAAAGGAAAATTC
  AGGAAGTTTGGAGACTATAAATGAGCCTATTCCCCAGCCTATGCCTTTTCCGCCCAGCATAATCAGATCA
  GGATCCCCAAAATTGGATCCTTCTGAGGTCTACCTGAAATCTAAGACTTTATATGAAGATAAACCACCAA
  ACACCAAAGATCTGGATGTCTTTCTTCGGAAACAAGAATCAGGGTTTGGCTTCAGGGTGCTAGGAGGAGA
  TGGACCTGACCAGTCTATATATATTGGGGCCATCATTCCCCTGGGAGCAGCTGAAAAAGATGGTAGGCTC
  CGTGCTGCTGATGAATTGATGTGTATTGATGGGATTCCTGTTAAAGGAAAATCACACAAGCAAGTATTAG
  ACTTAATGACTACTGCTGCTCGAAATGGACACGTGCTACTAACTGTCAGGAGGAAGATCTTCTATGGAGA
  GAAACAACCCGAGGACGAAAGCCATCAAGCCTTCTCACAGAACGGATCTCCTCGCCTGAATCGGGCAGAG
  CTTCCAACCAGGTCTGCCCCACAGGAGGCCTATGATGTCACCTTACAGAGGAAAGAAAATGAAGGGTTTG
  GTTTTGTCATCCTCACCTCTAAAAGCAAGCCGCCCCCAGGAGTTATTCCTCATAAAATTGGCCGGGTCAT
  AGACGGAAGTCCAGCTGATCGTTGTGGAGGACTGAAAGTTGGAGATCACATCTCTGCTGTGAATGGGCAG
  TCCATTGTCGACCTATCCCATGATAATATTGTTCAGCTCATCAAAGATGCTGGAGTCACCGTCACGCTGA
  CAGTGGTTGCTGAAGAAGAGCATCATGGTCCACCATCAGGAACAAACTCAGCCAGGCAGAGCCCAGCACT
  ACAGCACAGGCCCATGGGACAAGCACAAGCCAACCACATACCTGGGGACAGAATTGCCCTAGAAGGTGAA
  ATTGGGAGAGATGTCTGCAGTTCTTACAGACATTCCTGGTCTGACCATAAGCACCTTGCACAGCCTGACA
  CTGCAGTGATTTCAGTTGTGGGCAGTCGGCACAATCAGAGCCTTGGCTGTTACCCTGTGGAGCTGGAGAG
  AGGCCCTCGAGGCTTTGGGTTCAGCCTCCGCGGAGGGAAGGAGTACAACATGGGGCTGTTCATCCTGCGC
  CTGGCCGAGGACGGGCCCGCCATCAAAGACGGCAGGATTCACGTTGGTGACCAGATCGTTGAAATCAATG
  GGGAACCGACACAAGGCATCACACACACTCGAGCAATTGAGCTCATTCAGGCTGGTGGGAATAAAGTCCT
  CCTCCTTCTGAGGCCAGGAACTGGCTTGATACCTGACCACGGTGATTGGGATACTAATAGTCCTTCATCT
  TCCAATGTCATTTATGATGAACAGCCGCCACCTCTCCCATCTTCACATTCTGCTTCCACATTTGAAGAGT
  CTCATGTGCCAGCAACTGAAGACTCTCTGACTAGAGTTCAGATATGTGAAAAGGCAGAAGAATTAAAGGA
  CACTGTACAAGAAAAGAAAAGCACTTTAAATGGAAGCCAGCCTGAGATGAAGTATCAGTCTGTCCACAAA
  ACTATGAGTAAGAAGGATCCACCCAGAGGTTCCGGGCATGGCGAGAAGAGTCGACTAAAAGGAGAAAATG
  GTGTTACACGGAGAGGTAGATCTGCTAGTCCCAAAAAGTCAGTTAACCGACATTCAGAGGAACATTTGGA
  AAAGATTCCCAGGCCCCTAAAAAGTGATCCCAAAGAAAAATCTAGAGACCGCTCACTGAGCCCTAGGAAA
  GGAGAAAGTAAAGGTCGGCTCACCATTAAGGCGGGCTCTGGACAAGATCCCAATAGAAAAGACAGAGGAC
  GGTCTTCTAGCCCCAAGAAGCAGCAAAAGATAGGAGGCAACAGCCTGTCAAACACTGAAGGCAAATTATC
  AGAGGCTGGGAGCCGAAGAGCAGCAGGCCACCCTAGAGACAGCACTGAACAGCTCCCAGATGGGAGAGAA
  AAGTCAGGGGTCAGCAGGAAAGATCTGAAGCAGAGTCAGCCAGGAAAAACCAGAACCAAGTCTCCAGAGA
  AAAAGAGCAGTAAAGTTGATGAAACATCTCTCCCATCCAAAAAGACTAGCAGCACTGCTGGTAGGGTAGT
  ATCTGAAAAGGAAAAAGGAAAGAAGCCAACAGCAGGAGAAACAAGTAGAGAAACAGTAGAGCACACCCAG
  ATCTCAGCAAAGCAGCTCAAGCAGGAAGCTCAAGAGAAGACGGCATTAGGTAACGCAGACGATCACAAAG
  GCAGAGAATCAGAGGTCACGGACAGATGCAGGGAGCGTGCGGGGTGCACACCTCAAAGTAGCTCTCTAGT
  CAAGAAAGCACCCATCACTCCAGGACCCTGGAGGGTGCCACGTGCAAATAAAGTCACAGGCACTACTGGC
  ATGGCTGACAAACAGCTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001159354
• Insert Size: 4431 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001159354.1, NP_001152826.1
• RefSeq Size: 6581 bp
• RefSeq ORF: 4431 bp
• Locus ID: 99470
• Cytogenetics: 3 45.52 cM
• Gene Summary: Acts as a scaffolding protein at cell-cell junctions, thereby regulating various cellular and signaling processes. Cooperates with PTEN to modulate the kinase activity of AKT1. Its interaction with PTPRB and tyrosine phosphorylated proteins suggests that it may link receptor tyrosine phosphatase with its substrates at the plasma membrane. In polarized epithelial cells, involved in efficient trafficking of TGFA to the cell surface. Regulates the ability of LPAR2 to activate ERK and RhoA pathways. Regulates the JNK signaling cascade via its interaction with FZD4 and VANGL2.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.