Ift140 (NM_134126) Mouse Untagged Clone

CAT#: MC224521

Ift140 (untagged) - Mouse intraflagellar transport 140 homolog (Chlamydomonas) (Ift140), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ift140
• Synonyms: AI661311; mKIAA0590; Tce5; Wdtc2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224521 representing NM_134126
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCTGTATTTTGACCACCGAATCAAAGCTCCAGACACACCCAGCTCTCCCTCCCACATCACCTGGC
  ACCCCACCCACCCATTCCTGGCAGTCGCATCTATAAGCCCATCTTCTGGAGGCAACGTAGACATCTACCT
  TGAGCAAGGTGAGCCCGTGCCTGACACCCACATTGAGAGGTCTTTCCAGGCCACTTCACTGTGCTGGCAC
  CCAACAAGGCTTATCCTGGCTATTGGCTGGGAAACTGGAGAAGTGATAATGTTTAATAAGCAGGACAAGG
  AACAGCACACAGTGCCCCTGCCACATACGACTGACATTGCCATCCTCAGTTGGAGCACCAGTGGGAGCTG
  CCTGGTATCTGGAGATAAGCTTGGGGTCCTGCTCTTGTGGAGATTGGACCAGCGAGGCCGAGTACAGGGA
  ACACCACTCCTGAAACACGAATATGGGAAAGCACTCACCCATTGCATCTTCCGGCTCCCCCCTCCTGGGG
  AGGATCTGGTTCAGTTGGCAAAGGCAGCAGTAAGCGGGGATGAGAAAGCCCTGGACATGTTTAACTGGAG
  AAAAAGCAGCTTTGGAAGTTTCCTGAAGACGGGGTCTCAAGAGGGGCTGTCTTTCTTTGTCAGTCTCATG
  GATGGCACAGTGCACTATGTGGATGAAAAGGGCAAGACTGCCCAGGTGGCATCCACAGACAGCTCCATCC
  AGACCCTGTTCTACATTGAGAGGAGAGAGGCACTGGTTGTGGTCACAGAGAACCTTCTCCTGTCTCTGTA
  TGTGGTGACTCCTGAGGGAGAAGCTGAAGAAGTCATGAAGGTAAAGTTGAGTGGGAAGACAGGCTGCCGG
  GCAGATATCACTTTGATAGAGGGCAGCCTTCTGGTGACAGCTATTGGGGAGCCTGTGCTCAGGTTCTGGG
  ACTTAGAACGGGGAGAGAATTACATACTGAGTCTACAGGAGAAGTTTGGCTTTGAAAAGGGAGAGAGTAT
  AAACTGTGTGTGTTTCTGTAAAGCCAAAGGTCTCCTAGCAGCAGGTACCAATAAAGGACGAGTAGCTATG
  TGGAAAAAAGTGCCAAGCTTCCCAAATGGCCGTGGGGCGGAGGGCAAGGACATGTGGGCCCTTCAGACCC
  CTACTGAGCTTGAAGGGAACATCACACAGATAAAGTGGGGCTCCAGGAAGAACCTTCTGGCAGTGAGCAG
  CACCGAGTCTGTGTCCATCCTCAGTGAGCAAGCCATGTCGTCACACTTCCACCAGCAAGTAGCTGCCGTG
  CAGATCTCCCCGAGCCTAGTCAACGTGTCTTTCCTGTCCACGGGGGGCACACACAGCCTGCACACCGACA
  TGCACATCAGTGGAGTGTTCGCCACCAAGGATGCTGTGGCTGTCTGGAATGGAAAACAGGTGGCGATCTT
  TGAACCTTCAGGATCCACCTTAAGGAATGCAGGGACATTCCTGTGTGAAACATCAGTGCTAGCCATGCAT
  GAGGAAAGCATTTACACCGTGGAGCCAAACCGACTCCAAGTCCGGACCTGGCAGGGGACTGTCAAACAAC
  TTCTGCTATTCTCTGAGACCGAGGGGAGCCCCTGCTTCCTGGACGTCTGTGGGACTTTCCTGGTTGCAGG
  GACAGATTTAGCTCATTTTAAAAGTTTTGATCTTTCCAGAAGGGAAGCAAAAGTGCACTGCAGCTGCAAG
  AACCTGGCCCAGCTGGTCCCTGATGTGGGGAGCATCACTTCTCTGAGATGCAATGCAAATGGGAACAAGA
  TCAGCATCCTCCTTAGCAAGGTCAACAACAGTCCTGATTCCAAAATCTACATCTATGATGTTGAAATGGA
  CACAGTGAACGTCTTCAACTTCACAACTGGACAGATTGGACAGATACAGACTCTGCCCTTTAACGAGCCG
  CCGACTAATGAGACCCGTTCCTTTATGGACAAGAGCCTAGCAGGTTACACGCCGGTGAACCATTTCTGGG
  ATCAGAGTGAGCCCAGGCTCTTTGTGTGCGAGGCCTTGCAGGAGGCCCCTGGAGCCCAGCCACAGGCTGT
  AGACAAGCAGCCCCGTGTAGAGGAGGGCACATGCCACAAGGAAGAAGTGTTGATTCTCTCCTTCTTTGCT
  TCTGAAGAGCATGGCTTTCTGCTCCATGACAGCTTCCCCCGGCCTTCAACCTACCAGAGCCTTCTGGGCA
  TGGAAGTGCCTCACTATTACTTCACAAAAAAGCCTGGAGAAGCAGACAAAGAAGACAGGGTGGATTCTGG
  GTACTACCACATCCCTCAGATGGTAGCCAAGAGGCCCCTACGAGACTTTGTGGGGCTGGAGGACTGTGAC
  AAGTCTACTCGGGATGCCATGCTCAACTTTAGCTTCTTTGTCACCATTGGAGACATGGATGAAGCTTTCA
  AGTCTATCAAACTCATCAAGAGTGAGGCTGTCTGGGAGAACATGGCACGCATGTGTGTGAAGACTCAACG
  GCTAGATGTTGCCAAGGTGTGCCTAGGGAACATGGGCCACGCCCGTGGGGCCCGAGCACTGCGGGAAGCT
  GAGCAGGAGCCAGAACTGGAAGCCAGAGTGGCCATGCTGGCCATACAGCTGGGCATGCTGGAGGAGGCAG
  AACAACTGTATAAGAAGTGCAAACGCTACGACCTCCTGAACAAGTTCTACCAGGCCTCAGACCAGTGGCA
  GAAAGCTGTGGAGGTTGCGGAGCTCCACGACCGTGTCCACCTGCGCACCACCTACTACAACTACGCCAAG
  CACCTGGAGGCCAGCGCTGACTGTGGCCAGGCCCTCAGTTATTATGAGAAGTCAGACACCCACCGTTTTG
  AGGTACCTAGGATGCTCTCAGAAGACCTGCAGTCCCTGGAGCTCTACATCAATAGGATGAAGGACAAGAC
  CCTCTGGAGATGGTGGGCCCAGTATCTTGAGAGCCAGGCAGAGATGGACACTGCACTGCGCTACTATGAG
  CTGGCGCAGGACTACTTCTCCCTGGTCCGTATTCACTGCTTCCAGGGCAACATTCAGAAGGCTGCTGAAA
  TAGCCAACGAGACTGGAGACTGGGCTGCATCCTACCACCTTGCCCGGCAGTACGAGAGCCAGGATGAGGT
  AAAACAGGCAGTGCACTTCTACACTCGGGCACAGGCTTTCAACAACGCCATCCGCCTGTGCAAGGAGAAC
  GGCTTAGATGACCAGCTCATGAACTTGGCGCTGTTGAGTTCCCCAGAGGACATGATTGAGGCAGCCCGGT
  ATTATGAGGAGAAGGGCGAGCAGATGGACAGGGCTGTCATGCTGTACCACAAGGCTGGACACTTTTCCAA
  GGCCCTGGAGCTGGCCTTCACCACCCAGCAGTTTGCAGCACTACAGCTCATAGCCGAGGACCTGGATGAG
  AAGTCAGATCCTGCTCTCCTGGCCCGCTGCTCAGACTTCTGCATTGAGCACAGGCAGTTTGAAAAGGCTG
  TGGAGCTTCTCCTGGCTGCCAAGAAGTACCATGAGGCTCTGCAGCTGTGCCTTGAGCAGAACATGACCAT
  CACTGAGGACATGGCAGAGAAGATGACTGTGTCCAAGGACTCAAAGGACATGTCTGAGGAGTCACGGCGT
  GAGCTTCTGGAGCAGATTGCCAACTGCTGTATGCGCCAGGGCAACTATCACCTGGCCACCAAGAAGTACA
  CACAGGCTGGGAACAAGCTAAAGGCCATGAGAGCACTGCTCAAATCTGGAGACACAGAGAAAATTGTGTT
  CTTTGCGGGTGTCTCAAGACAGAAGGAAATCTACATCATGGCTGCCAATTACCTACAGTCCCTGGACTGG
  CGGAAGGAGCCAGAGATCATGAAGAGTATCATTAGCTTCTACACCAAAGGGCGGGCCTTGGACCTCCTGG
  CTGGCTTCTATGATGCCTGTGCCCAGGTGGAGATTGACGAATACCAGAACTATGACAAGGCCCATGGAGC
  ACTGACTGAGGCCTATAAGTGCCTGTCCAAAGCCAAGACCAAGAACCCCCTGGACCAGGAGACCAAGCTA
  GCCCAGCTACAGAGCAAGATGACACTGGTGAAGAGGTTCATCCAGGCCCGAAGGACATACACAGAGGACC
  CCAAAGAGTCCCTCAGGCAGTGTGAGCTGCTCCTAGAAGAGCCAGACCTGGACAGCACCATCCGTGTCGG
  AGACGTCTATGGCTTCCTGGTGGAACACCATGTACAGATGGAGGAATACCAGATGGCCTATAAGTATCTT
  GAGGAGATGAGGAAAAGACTTCCTTCCGCCAACATGTCATACTATGTGGACCAGCGCACTGTGGACACTG
  TGCACCAGGGCCTGGGCCTCCTCCCTCCATCTCGTATCATGCCTGAGCGGGTCCGCCACAATAGCATGGA
  GGACCATAAGGAGGTGTACGAGGAGGTGATAGAAGAGGTGGACAATGACCCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_134126
• Insert Size: 4395 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_134126.3, NP_598887.3
• RefSeq Size: 5814 bp
• RefSeq ORF: 4395 bp
• Locus ID: 106633
• UniProt ID: E9PY46
• Cytogenetics: 17 A3.3
• Gene Summary: Component of the IFT complex A (IFT-A), a complex required for retrograde ciliary transport and entry into cilia of G protein-coupled receptors (GPCRs) (By similarity). Plays a pivotal role in proper development and function of ciliated cells through its role in ciliogenesis and/or cilium maintenance (PubMed:22282595). Required for the development and maintenance of the outer segments of rod and cone photoreceptor cells. Plays a role in maintenance and the delivery of opsin to the outer segment of photoreceptor cells (PubMed:24619649).[UniProtKB/Swiss-Prot Function]