Pola1 (NM_008892) Mouse Untagged Clone

CAT#: MC224516

Pola1 (untagged) - Mouse polymerase (DNA directed), alpha 1 (Pola1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pola1
• Synonyms: AW321876; Pola
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224516 representing NM_008892
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCCCATGCACGAAGAGGACTGTAAACTGGAGGCAAGCGCTGTGTCAGATTCAGGGAGTTTTGCAG
  CTTCCCGAGCTCGGCGAGAAAAGAAATCAAAGAAAGGACGTCAAGAAGCTTTAGAGAGACTGAAAAAGGC
  TAAAGCTGGGGAGAAGTATAAATACGAAGTTGAGGACCTCACAAGTGTTTATGAAGAAGTTGATGAAGAG
  CAGTATTCGAAACTGGTTCAGGCCCGTCAGGATGATGACTGGATTGTGGATGACGATGGGATAGGCTATG
  TGGAAGATGGCAGAGAGATTTTTGATGATGACCTTGAGGATGATGCACTTGACACCTGTGGAAAAGGAAG
  TGATGGCAAAGCACACAGAAAGGACAGGAAGGATGTAAAGAAACCTTCAGTGACAAAACCAAACAATATC
  AAGGCAATGTTCATTGCTAGTGCCGGGAAGAAAACTACAGATAAAGCAGTAGACTTGTCCAAGGATGATC
  TGCTAGGTGACATTCTACAGGATCTAAACACTGAGACAGCTCAAATAACTCCACCACCTGTATTGATACC
  AAAGAAAAAAAGATCTACTGGGGCTTTGCTCAACCCTTTTTCAGTGCACACCCCTAAGGCAATTCCTTCA
  GGAAAACCTGCTTCCCCTGTCCTCAGGAATGAGCCTCTGTTAACTCCCATTCCTCTTAAACGTGCTGAAC
  TTGCTGGAGAGCTGGCTCAGCCAGAATGTCCAGAAGATGAGCAGGAGCTAGGGGTAATGGAGTTTGAAGA
  CGGTGACTTTGATGAGTCCATGGACACAGAGAAGGTAGATGAGAAGCCTGTGACTGCTAAGACTTGGGAC
  CAAGAGACTGAGCCAGTAGAAAGGGTGGAACATGAGGCAGATCCTGAGAGGGGAACCACATCTTACTTAG
  AAAATTTTCTGCCAGATGTCTCTTGTTGGGACATTGATCAGGATGATGAAAGCATACCTCAGGAAGTTCA
  AGTGGATTCCAGTAATCTCCCATTGGTAAAAGGGGCAGATGATGAACAAGTGTTTCAGTTTTATTGGCTG
  GATGCTTATGAAGATCCATACAACCAACCAGGTGTGGTTTTTCTGTTTGGCAAAGTGTGGATTGAATCAG
  TCAAAACCCATGTGAGCTGTTGTGTTATGGTGAAAAATATTGAGCGAACACTCTACTTCCTTCCCCGTGA
  AATGAAATTTGACCTAAACACAGGGAAAGAAACAGCAATTCCAGTTACAATGAAGGATGTTTATGAAGAA
  TTTGATTCAAAAATTTCAGCAAAATATAAAATAATGAAGTTCAAGTCCAAGATAGTGGAAAAGAACTATG
  CTTTTGAGATACCTGATGTTCCAGAAAAATCTGAGTACCTGGAAGTTAGATACTCGGCAGAAGTGCCACA
  GCTTCCACAGAATTTAAAAGGAGAAACATTTTCTCATGTGTTTGGAACTAACACGTCTAGCTTGGAACTG
  TTCTTGATGAATAGGAAGATCAAAGGACCTTGTTGGCTTGAAGTAAAAAATCCACAACTCTTGAATCAGC
  CAATCAGTTGGTGTAAATTTGAGGTAATGGCTTTGAAACCAGACCTGGTGAATGTAATTAAGGATGTCAG
  TCCTCCTCCACTTGTGGTGATGTCTTTCAGCATGAAGACAATGCAGAATGTACAGAACCATCAACATGAG
  ATTATTGCTATGGCGGCTTTGGTTCATCACAGTTTTGCATTAGATAAAGCACCTCCAGAGCCTCCCTTTC
  AGACACATTTCTGTGTTGTGTCTAAACCAAAGGACTGTATTTTCCCATGTGATTTCAAAGAAGTGATTTC
  GAAAAAGAATATGAAGGTTGAGATCGCTGCAACAGAAAGAACCTTGATAGGATTTTTCCTTGCAAAAGTT
  CACAAAATTGACCCTGACATTCTTGTGGGTCATAATATTTGTAGTTTTGAACTGGAAGTGCTGCTGCAAA
  GAATTAATGAGTGCAAGGTTCCTTACTGGTCCAAGATAGGTCGGCTGAGGAGATCCAACATGCCAAAACT
  TGGGAGCCGGAGTGGATTTGGTGAAAGAAATGCTACCTGTGGCCGAATGATATGTGATGTGGAAATTTCA
  GCAAAAGAATTGATTCATTGTAAAAGCTACCATTTGTCTGAACTTGTTCAGCAGATTCTGAAAACTGAGA
  GGATTGTAATTCCAACAGAAAATATACGAAACATGTATAGTGAATCTTCTTATCTGTTGTACCTGTTGGA
  ACATATCTGGAAAGATGCCAGATTCATTTTGCAGATCATGTGTGAGCTAAATGTTCTTCCATTAGCATTG
  CAGATCACTAACATCGCTGGGAACATTATGTCTAGGACACTGATGGGTGGACGATCTGAGCGAAATGAGT
  TTTTGTTGCTTCATGCATTTTATGAAAACAACTATATTGTGCCTGACAAGCAGATTTTCAGAAAACCTCA
  GCAAAAACTGGGAGATGAAGATGAGGAAATTGATGGGGACACCAATAAATACAAGAAAGGGCGTAAGAAA
  GCAACTTACGCTGGAGGCTTGGTTTTGGACCCCAAAGTTGGTTTTTATGACAAGTTCATTTTGCTCCTGG
  ATTTCAACAGTTTATATCCTTCCATCATTCAGGAATTTAACATTTGTTTTACAACAGTGCAAAGAGTTAC
  TTCAGAGGTGCAGAAAGCTACAGAGGATGAGGAACAAGAACAGATCCCTGAGTTGCCAGATCCAAACTTA
  GAGATGGGCATTTTGCCCAGAGAGATTCGGAAACTGGTAGAGCGGAGAAAACAAGTCAAACAGCTAATGA
  AACAGCAAGATTTAAATCCAGACCTTGTTCTTCAGTATGACATCCGACAGAAGGCTTTGAAGCTCACAGC
  AAACAGTATGTATGGTTGCCTGGGATTTTCGTACAGCAGATTTTATGCTAAACCACTGGCTGCCTTGGTG
  ACATACAAAGGAAGGGAGATTTTGATGCATACGAAAGACATGGTACAGAAGATGAATCTTGAAGTTATTT
  ATGGAGACACAGATTCAATTATGATAAATACCAATAGCACCAACCTGGAAGAAGTATTTAAGTTGGGAAA
  CAAGGTAAAAAGTGAAGTGAATAAATTGTACAAACTGCTTGAAATAGACATTGATGCTGTTTTCAAGTCT
  CTGCTGTTGCTGAAGAAAAAGAAATATGCTGCTCTAGTTGTTGAACCAACATCAGATGGGAATTACATTA
  CCAAGCAGGAGCTGAAAGGATTAGATATAGTTAGAAGAGACTGGTGTGATCTTGCCAAGGACACTGGAAA
  CTTTGTCATTGGCCAGATTCTTTCTGATCAAAGCCGGGACACCATTGTGGAAAACATTCAGAAGAGGTTA
  ATAGAAATTGGAGAAAATGTGCTAAATGGCAGTGTCCCAGTGAGCCAGTTTGAAATTAACAAGGCATTGA
  CAAAGGATCCCCAGGATTACCCTGATAGAAAAAGCTTACCTCATGTACATGTTGCACTGTGGATAAATTC
  TCAAGGAGGCAGGAAGGTGAAAGCTGGTGATACGGTGTCCTATGTTATTTGTCAGGATGGGTCAAACCTC
  ACTGCAACTCAGAGGGCCTATGCCCCAGAACAATTGCAGAAACTTGACAATTTAGCCATCGACACCCAAT
  ACTACTTGGCCCAGCAGATCCACCCTGTTGTGGCTCGGATCTGTGAGCCAATAGATGGAATTGATGCTGT
  ACTCATTGCATTATGGCTAGGACTTGACTCCACTCAATTTAGAGTTCATCAGTATCACAAAGATGAAGAG
  AATGATGCCCTCCTTGGTGGTCCAGCACAGCTAACTGATGAAGAGAAATACAAGGATTGTGAAAAATTCA
  AATGCCTTTGTCCTTCATGTGGAACTGAAAATATTTATGATAATGTCTTTGAAGGCTCGGGACTGGATAT
  GGAACCCAGTTTGTATCGTTGCAGTAATGTTGATTGTAAGGTTTCACCGTTGACCTTCATGGTACAGCTG
  AGCAACAAATTGATCATGGACATTAGACGGTGCATTAAAAAGTACTATGATGGCTGGTTGATATGTGAAG
  AGCCAACTTGTTGCAGTCGCCTTCGACGTCTTCCCCTTCATTTCTCCCGAAATGGGCCTCTGTGTCCAGT
  CTGCATGAAAGCTGTGCTTAGACCAGAGTATTCTGACAAGTCCTTGTACACCCAGCTGTGCTTTTACCGG
  TACATTTTTGATGCGGACTGTGCACTGGAAAAACTTACTGAACATGAGAAAGATAAATTGAAGAAGCAGT
  TTTTCCCTCTCCGAGTTCTTCAGGACTACAGAAAAGTCAAGAACATAGCAGAGCAGTTCTTGTCCTGGAG
  TGGCTACTCTGAAGTGAACCTCAGCAAGCTCTTCGCCAATTACGCTGGGAAGTCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008892
• Insert Size: 4398 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008892.2, NP_032918.1
• RefSeq Size: 5350 bp
• RefSeq ORF: 4398 bp
• Locus ID: 18968
• UniProt ID: P33609
• Cytogenetics: X 41.06 cM
• Gene Summary: Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes. In the cytosol, responsible for a substantial proportion of the physiological concentration of cytosolic RNA:DNA hybrids, which are necessary to prevent spontaneous activation of type I interferon responses.[UniProtKB/Swiss-Prot Function]