Ncoa2 (NM_008678) Mouse Untagged Clone

CAT#: MC224515

Ncoa2 (untagged) - Mouse nuclear receptor coactivator 2 (Ncoa2), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ncoa2
• Synonyms: bHLHe75; D1Ertd433e; GRIP-1; Grip1; KAT13C; NCoA-2; SRC-2; TIF2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224515 representing NM_008678
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGGGATGGGAGAAAACACCTCTGACCCGTCCAGGGCAGAGACCAGAAAACGCAAGGAATGTCCCG
  ACCAGCTCGGACCCAGCCCCAAAAGGAGCACTGAGAAACGGAACCGCGAGCAGGAGAATAAGTACATAGA
  GGAGCTGGCCGAGCTGATCTTCGCAAACTTTAATGATATTGACAACTTCAACTTCAAACCTGACAAATGT
  GCCATCCTAAAAGAAACTGTGAAGCAGATCCGCCAGATCAAAGAGCAAGAGAAAGCAGCAGCTGCCAACA
  TAGATGAAGTGCAGAAGTCAGATGTGTCGTCCACGGGGCAGGGTGTCATCGACAAGGATGCACTGGGGCC
  CATGATGCTTGAGGCCCTCGATGGGTTCTTCTTCGTTGTGAACCTGGAAGGCAGTGTGGTGTTCGTGTCA
  GAGAATGTGACACAGTATCTACGGTATAACCAAGAAGAGCTGATGAACAAGAGTGTCTACAGCATCCTGC
  ATGTCGGGGACCACACTGAATTTGTCAAGAACCTGCTGCCAAAGTCCATGGTGAATGGAGGATCCTGGTC
  TGGAGAACCTCCCAGGCGGAGCAGCCATACCTTCAACTGTCGCATGCTGGTGAAGCCTTTGCCAGATTCA
  GAAGAGGAAGGCCATGATAGCCAGGAAGCCCATCAGAAATACGAGGCGATGCAGTGCTTCGCTGTGTCTC
  AGCCCAAGTCCATCAAAGAGGAAGGCGAAGATTTGCAGTCCTGCTTGATTTGTGTGGCACGAAGAGTCCC
  CATGAAGGAAAGACCAACTCTTCCCTCATCAGAAAGCTTTACCACCCGCCAGGACCTCCAAGGCAAGATC
  ACTTCACTGGACACTAGCACCATGAGAGCCGCCATGAAGCCGGGCTGGGAAGATCTGGTAAGAAGATGCA
  TTCAGAAGTTCCACACACAGCATGAAGGGGAGTCTCTATCATATGCCAAGAGGCATCACCATGAAGTTCT
  GAGACAAGGGTTGGCGTTCAGTCAGATCTATCGTTTTTCTTTGTCTGATGGCACTCTCGTTGCTGCACAA
  ACCAAGAGCAAACTCATCCGTTCTCAGACTACTAATGAGCCTCAGCTTGTAATATCTTTACACATGCTTC
  ACAGAGAGCAGAATGTATGTGTAATGAATCCGGATCTGACTGGACAAGCGATGGGGAAGCCATTGAATCC
  AATTAGCTCTAGCAGCCCTGCCCACCAGGCCCTGTGCAGTGGGAACCCAGGTCAGGACATGACCCTCGGT
  AGCAATATAAATTTTCCCATGAATGGCCCAAAGGAACAAATGGGCATGCCTATGGGCAGGTTTGGTGGTT
  CTGGGGGCATGAACCATGTGTCAGGCATGCAAGCAACCACTCCTCAGGGTAGTAACTATGCACTCAAAAT
  GAACAGTCCCTCGCAAAGCAGCCCCGGCATGAACCCGGGGCAAGCCAGCTCCGTGCTCTCCCCAAGGCAG
  CGCATGAGCCCCGGCGTGGCTGGCAGTCCTCGCATCCCACCCAGTCAGTTTTCCCCTGCAGGAAGCTTGC
  ATTCCCCTGTGGGAGTTTGCAGCAGCACAGGAAATAGCCATAGTTATACCAACAGTTCCCTCAATGCACT
  GCAAGCCCTCAGCGAGGGCCATGGGGTCTCACTCGGGTCCTCGCTGGCTTCACCGGACCTAAAAATGGGC
  AATTTGCAAAACTCCCCAGTTAATATGAATCCTCCCCCACTCAGCAAGATGGGAAGCTTGGACTCCAAAG
  ACTGTTTTGGACTTTATGGGGAGCCCTCAGAAGGTACAACTGGACAAGCAGAGGCCAGCTGCCATCCTGA
  AGAACAAAAGGGGCCCAATGATTCCAGCATGCCCCAGGCGGCCAGCGGGGACAGGGCTGAGGGACACAGC
  CGGCTGCATGACAGCAAAGGGCAGACCAAACTCCTGCAGCTGCTGACCACCAAGTCCGACCAGATGGAGC
  CTTCACCCTTGCCCAGCTCCTTGTCGGACACAAACAAGGACTCAACAGGGAGCTTGCCTGGGCCTGGGTC
  CACGCATGGCACCTCGCTCAAGGAGAAGCATAAGATTTTGCACAGACTCTTACAGGACAGCAGTTCCCCT
  GTGGACTTGGCCAAGCTGACAGCAGAAGCCACAGGCAAAGAGCTGAGCCAGGAGTCCAGCAGCACAGCTC
  CTGGGTCGGAAGTGACTGTCAAACAGGAGCCAGCGAGCCCCAAGAAGAAAGAGAATGCACTACTGCGCTA
  TTTGCTCGACAAAGATGATACTAAAGATATTGGTTTACCGGAAATAACCCCCAAACTCGAGCGACTGGAC
  AGTAAGACAGATCCTGCCAGTAACACAAAGTTAATTGCTATGAAAACTGTGAAGGAGGAGGTGAGCTTTG
  AGCCCAGTGACCAGCCTGGCAGCGAGCTGGACAACTTGGAAGAGATTTTGGATGATTTGCAGAACAGTCA
  GTTACCACAGCTTTTCCCAGACACAAGGCCAGGAGCTCCTACTGGGTCAGTTGACAAGCAAGCCATCATC
  AATGACCTCATGCAACTCACAGCTGACAGCAGTCCCGTCCCACCTGCCGGAGCCCAGAAGGCAGCACTGC
  GCATGTCACAGAGCACTTTTAATAACCCACGACCAGGGCAACTGGGCAGGTTATTGCCAAACCAGAACTT
  ACCACTTGACATCACTTTGCAAAGCCCAACTGGTGCTGGACCTTTCCCACCAATCAGAAACAGTAGCCCC
  TACTCAGTGATACCTCAGCCAGGAATGATGGGTAACCAAGGGATGCTAGGAAGCCAAGGAAACTTAGGGA
  ACAATAGCACAGGAATGATTGGCAGCAGCACTTCCCGGCCCAGCATGCCTTCTGGGGAATGGGCACCACA
  GAGTCCAGCTGTGAGAGTCACTTGTGCTGCTACCACTGGTGCCATGAACCGACCAGTCCAAGGAGGCATG
  ATTCGGAACCCAACAGCCAGCATCCCCATGCGAGCCAACAGCCAGCCTGGCCAAAGACAGATGCTTCAGT
  CTCAGGTCATGAACATAGGCCCTTCTGAGTTAGAGATGAACATGGGAGGACCTCAGTATAATCAACAGCA
  GGCCCCTCCGAACCAAACTGCCCCGTGGCCTGAGAGCATCCTGCCTATAGACCAGGCATCGTTTGCCAGC
  CAGAACAGGCAGCCCTTCGGCAGCTCCCCTGATGACCTGCTGTGTCCACATCCTGCAGCAGAGTCGCCAA
  GCGATGAGGGCGCTCTTCTTGACCAGCTGTATCTGGCCTTGCGGAACTTCGATGGCCTTGAGGAGATTGA
  TAGAGCTCTGGGGATACCAGAACTGGTCAGCCAGAGCCAAGCTGTGGATGCAGAGCAGTTCTCAAGTCAG
  GAGTCCAGCATAATGCTGGAGCAGAAGCCCCCCGTTTTCCCACAGCAGTACGCATCTCAGGCACAAATGG
  CCCAGGGTGGCTATAATCCCATGCAAGATCCAAACTTTCACACCATGGGACAGCGGCCAAATTACACCAC
  ACTCCGTATGCAGCCACGGCCAGGCCTCAGGCCCACAGGCATTGTGCAGAACCAGCCAAACCAACTGAGA
  CTTCAGCTTCAGCACCGCCTCCAAGCACAGCAGAATCGCCAGCCGCTTATGAATCAGATCAGCAGTGTTT
  CCAATGTGAACCTGACTCTGAGGCCTGGAGTGCCCACTCAGGCTCCTATTAATGCACAGATGCTGGCCCA
  GAGGCAGAGGGAAATCCTCAACCAACATCTTCGGCAGAGACAGATGCAGCAGCAGGTGCAGCAGCGGACT
  TTGATGATGAGAGGACAGGGCTTGAATGTGACCCCAAGCATGGTGGCTCCCGCTGGCCTACCAGCAGCCA
  TGAGCAATCCCCGGATCCCCCAGGCCAATGCCCAGCAGTTCCCATTTCCTCCGAACTACGGAATAAGTCA
  ACAACCTGATCCTGGCTTTACTGGGGCTACAACTCCCCAGAGTCCTCTAATGTCTCCCCGGATGGCACAT
  ACTCAGAGTCCCATGATGCAGCAGTCTCAAGCCAACCCAGCCTACCAGCCCACCTCAGACATGAATGGAT
  GGGCACAGGGGAGCATGGGTGGAAACAGCATGTTCTCACAGCAGTCCCCACCACACTTTGGGCAACAAGC
  AAACACCAGCATGTATAGTAACAACATGAACATCAGTGTGTCGATGGCAACCAACACGGGTGGCTTGAGC
  AGCATGAACCAGATGACAGGCCAGATGAGCATGACCTCAGTGACCTCCGTGCCTACGTCAGGACTGCCCT
  CCATGGGTCCCGAGCAGGTCAATGACCCTGCTCTGAGGGGAGGCAACCTTTTCCCAAACCAACTGCCTGG
  AATGGACATGATCAAGCAGGAGGGAGATGCATCTCGGAAATACTGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008678
• Insert Size: 4389 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008678.3, NP_032704.2
• RefSeq Size: 8286 bp
• RefSeq ORF: 4389 bp
• Locus ID: 17978
• UniProt ID: Q61026
• Cytogenetics: 1 4.12 cM
• Gene Summary: Transcriptional coactivator for steroid receptors and nuclear receptors. Coactivator of the steroid binding domain (AF-2) but not of the modulating N-terminal domain (AF-1). Required with NCOA1 to control energy balance between white and brown adipose tissues. Critical regulator of glucose metabolism regulation, acts as RORA coactivator to specifically modulate G6PC expression. Involved in the positive regulation of the transcriptional activity of the glucocorticoid receptor NR3C1 by sumoylation enhancer RWDD3. Positively regulates the circadian clock by acting as a transcriptional coactivator for the CLOCK-ARNTL/BMAL1 heterodimer (PubMed:24529706).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (a) represents the longer transcript and encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.