Skint5 (NM_001167878) Mouse Untagged Clone

CAT#: MC224474

Skint5 (untagged) - Mouse selection and upkeep of intraepithelial T cells 5 (Skint5), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Skint5
• Synonyms: OTTMUSG00000008560; Skint-5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224474 representing NM_001167878
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGCTGTAGGAATACCTCTTACCGCACACTGCGTTGTTTTGTTTCTCCTCCAGATGGTAGCACTGA
  GTTCAGAACAATTCACTGTGAATGGCTTAGAGAGTCCAGTCTTGGTTCCATTGGATGGAAACCTAGAACT
  CAGTTGTCAGTTGTCTCCACCACAGCAAGCAAAACACATGGAAATTCGCTGGTTCAAGAATCGCTATTCA
  GAGCCAGTATACCTGTATAGAAATGGTAAAGACCTTTTTGGAGAAATTGTCTACAAGTATGTAGAGAGAA
  CTGAACTCCTGAAAGATGACATCGGGAAAGGGAAAGTAACCCTCAGGATCTTTAAAGTAACTAGTGTTGA
  CAGTGGGTCCTACCACTGCTTCTTCAAAGATGGCAAGTTCTATGAAGAGCATATCATAGACGTCAAGGTC
  ACAGCTACAAGCTCAGACATACAGATTCTTATGCATCCCCCAAATATCAAAGGTGTGAGGTTGGAGTGTC
  ATTCAGGCGGTTGGTTCCCACAGCCTCACATGGAATGGAGAGATAGCAAAGGAGAGTACATTCCAGCTAC
  ATCAAAATCCCATTCACAGGATGAAAATGAATTTTTCAACATGACAATGGACCTTTTTATTAAATCCAAC
  TCTCACTGGAGTGCAACCTGCTATATTCAAAACTTTGTAACTCACCAAGAAGAAAGCATAAGTATTGTCC
  TACCAGGTGTGTGGGATTCATGGTGTCCTGCTTGGATAATGATTACTTTGCTGATTGTCATCCACATGAC
  TTACTATATTAAATTATACCGCACATATGCGACAAAAGAGATGCTTTTGAAAAGTATTCAATCAAGTTCT
  CAAAGTTCCACTGTGGATACAGAGAAAAAAGAGTGGCTGTTGAAAAGTATTCAATCAAGGATTCAAAGTT
  CCAGTGTGGATGCAGAGAAAAAAGAGTGGCTGTTGAAATTTATTCAATCAAGTATTCAAAGTTCCAGTGT
  GGATCTAGAAAAAAAAGAGTGGCTGTTGAAAAGCATTCAATCAAGTATTCAAAGTGCCATTGTGGATCGA
  GGAACAAAAGAGTGGCTGTTGGAAAGCATTCAATCAAGTATTCTAAGTTCCATTGTGGATCAAGGGACAA
  AAGTGTTGCTGTTGAAAAGTATTCAATCAAGCATTCAAAGTTCCACTCTGGATATAGAAACAAAGGAGTT
  GCTGTTGAAAAGCATTCAATCAAGTATTCAAAGTTCCATTATGGATCTAGGAACAAAAGAGTTGCTGTTG
  AAAATAATTCAATCGAGTATTCAAAGTTCCAGTGTGGATCTAGGAACAAAAGAGTTGCTGTTGAAAATAA
  TTCAATCGAGTATTCAAAGTTCCACTGTGGATCTAGGAACAAAAGAGTTGCTGTTGAAAATAATTCAATC
  GAGTATTCAAAGTTCCAGTGTGGATCTAGGAACAAAAGAGTTGCTGTTGAAAATAATTCAATCGAGTATT
  CAAAGTTCCACTGTGGATCTAGGAACAAAAGAGTTGCTGTTGAAAATAATTCAATCGAGTATTCAAAGTT
  CCAGTGTGGATCTAGGAGCAAAAATGGGGCTGTTGGAAAGCATTTTGTCAAGTATTCAAAGTTCCAATGT
  GGATCTAGAGACAAAAGTGTTGCTGTTGAAAAGTATTCAATCAAGTATTCAAAGTTCCACTGTGTATCTA
  GGAATAAAACAATGGCTGTTGGAAAGAATTGAATCAATTATTCAAAGTTCCAGTGTGTATCTAGATACAA
  AAGAGTTGCTGTTGAAAATTATTCAATCAAGTATTGAAAGTTACAGTGTGGATCTAGGGAACAAAGAGTT
  TCTGTTGAAAATTATTCTATCAAGTATCCAAAGTTCCAGTGTGGATCTAGGGACAAAAGAGTTGCTGGTG
  AAATTTTTTCAATCAAATATTCAAAGTTCCAGTGTGATTCCAGGGACAAAAAAGTTGCTGGTGAAAATTA
  TTCAATCAAGTCTTCAAAGTTCCAGTGTGGATCTAGGGACAAAAAAGGTGTTGTTGGATATTATTCAAAC
  AAGTATTCAAAACTCCAATGTGCATACAGAGAGAAAAGGGTTGCTGTTGAAAATTATTCAATCAAGTGTT
  CAAAGTTCCAGTGTGGATCAAGGGACAAAAGAGATGCTGTTGGAAATTATTCAATCAAGTATTCAAAATT
  CCAGTGTGAATCAATGGACAAAAGATTTGCTGCTGAAAATTATTCAATCAATTATTCAAAGTTCCAGTGT
  GGATCTAGGGACAAAAGGGTTCCTTTTGAAAATTATTCAATCAAGTATTCAAAGTTCCAGTGTGGATATA
  GGGACCAAATCCATGCTGTTGAAAAAAACAGGATTGATTTTGAAGAGTTCCATTGTGAATCCAGGGACAG
  AGTTGCTGTTTCAACTAATTGAATCAATTCGTCATAGTTCCAGTGTGAATTCAGAGACAAAAAAGATGCT
  GTCTGAAATAACTCAATCAACTGTTCAAAGTTCCAGTGTGAATCCAGTGACAGAAGAGATGATAGAGGAG
  AAATATCAATTGCTTCTTCAAAGTTCCAGTGTGAATCTAGAAGCAGAAAATATACTGAATGTTACAACCC
  AATGGATTCTTCAAATTTCCAGTGTGAATCAAGGAAAAGAAATGTTGCTCTTGGATAAAATTCAGCAGAT
  TCTTCAAATTTCAAGTGTGAATCCAGAAACAAAAGATTTGTTGTTGGAAAAAATTGAGTTGATTCTTCAA
  AATCCCGGTGTGCATCAAGAGACAAAAGATTTGCTGTTGGAAAGAATTCAGCTGATCCTTCAAAATTCAA
  GTGTGCAAAAAGAGACAAAATATTTGCTGTTGGAAAGAATTCGGTCGATCCTTCAAAGTTCGTGTGTGCA
  AAAAGAGACAAAAGATTTGCTGTTGGAAAGAATTCAGTCACTCCTTCAAAGTTCGTGTGTTCAAAAAGAG
  ACAAAAGATTTGTTGTTGGAAAAAATTCAGTTGATTCTTCAAAATTCCACTGTGCATCAAGAAACAAAAG
  ATTTGTTGTTGGAAAGAATTCAGTTGTTTCTTCAAAATTCCACTGTGGATCAAGAGACAAGTTATTTACT
  CCTGAAAAGAATTCAGTTGATTCTTCAAAGTTCCAGTGTGAAAAAAGAGACAAAAGAGTTCGTGTTGTAT
  ATAATTGAGTCGATTCTTCACAGTTCCAGTGTGCATCAAGAGACGAAAGATTTGCTGTTCGACAGAGTTC
  AGTTGATTCTTGAAAGTTCCAGTGTGCAGCAAGGGTTGAAATGTTTGCTGTTGAACATGATTCAGGTGAT
  TTTTCAAAGGACAAGTGTGCAAAAAGAGATGAAAGATTTGCTATTCAACAGAATTCCGTTGATTCTTGAA
  AGTTCCAGTGTGCAGCAAGAGACAAAAGATAAGCTGTTGGACATAATTCAGTCGATTCTTCAAAGGACCA
  GTGTACAAGAAGAGACAAAAGATAAGCTGTTGGACATAATTCAGTCGATTCTTCAAAGAAGCAGTGTGCA
  AAAAGATACGATAGATACGCTGTTGGACAGAATTCAGTTGATTCTTCAAAGAACCAGTGTGCAAAAAGAG
  ATGAAAGACTTCCTGCTGGACAGAATTAAGTCAATTCTTGAAAGTTTCAGTGTGCATCAAGAGACGAAAA
  AATTGCTGTTGAACAGAATTATGTCGATTCTTGAAAGTTCCACTGTGCATCAAGAGACAAAAAAATTGCT
  GTTGGACAGAATTCATTCGATTCTTAAAAGTTCCAGTGTGCAAAAAGAGACAAAAAACTTGCTGTTGGAC
  AGAATTCATTCGATTCTTAAAAGTTCCAGTGTGCAAAAAGAGTCCTGCAATAAGAGAAATCCTTTTTGGA
  AAAAGTATGCATTGGACCTCGGAATTTCAGTGTTCACAATTATAGTAGTCACTCTCATTATGCATTTAAA
  ACAAAGAGAAGCAGACCAACATTTTGAACTGAATACTTTGTGGTCAAAAGATACTAGTGTGATCCTGTGT
  GTGCTGATCATGTTTAACAACAGGCTCAAGGCATTGATATACTTCAGACTGTATGGATTTTCACCTCCAG
  GTAAAGCACATAAGTACATAGTAAACTACATATTAAGATTCTCACATCCCGTCTTTTGCATTGTCTACTC
  TGCAACAATCCTTTATATGTATCTGCAAATACAGAACAAAGATTCCTTATTTTCCTTGTATAATAGCTGG
  ATGGTAGAAATGGAAATGGTTCTAATATTTCTGCTCGTAATTTTCAATGTTAAAAACATTGCAACTGTAT
  TGTTATACTTTGACAGTACAACTCTACGACTATTCTTCTGGATTAAAGGCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001167878
• Insert Size: 4323 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001167878.1, NP_001161350.1
• RefSeq Size: 4434 bp
• RefSeq ORF: 4323 bp
• Locus ID: 242627
• UniProt ID: A7XUY5
• Cytogenetics: 4 D1
• Gene Summary: May act by engaging a cell surface molecule on immature T-cells in the embryonic thymus.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 1, resulting in a shorter protein (isoform 2), compared to isoform 1.