Fyco1 (NM_001110253) Mouse Untagged Clone

CAT#: MC224467

Fyco1 (untagged) - Mouse FYVE and coiled-coil domain containing 1 (Fyco1), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Fyco1
• Synonyms: 2810409M01Rik; Mem2; RUFY3; ZFYVE7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224467 representing NM_001110253
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTCTAGCAGCACTGAGACTCAGCTCCAGAGGATCATCCGTGATCTACAAGATGCAGCGACAGAAC
  TAAGCCATGAATTTAAGGAAGGCGGAGAGCCAATCACAGATGACAGCACCAGTTTGCACAAGTTTTCTTA
  CAAACTTGAGTATCTTCTGCAATTTGATCAGAAGGAGAAGGCGAGCCTCCTGGGAAGCAAGAAAGACTAC
  TGGGATTACTTTTGTGCGTGCCTAGCCAAGGTGAAAGGAGCAAATGATGGCATTCGATTTGTCAGGTCCA
  TCTCTGAGCTCCGAACATCTCTGGGGAAAGGGAGAGCCTTCATTCGCTACTCTTTGGTGCACCAGAGGCT
  GGCAGACACCTTGCAGCAGTGCTTCATGAACACTAAAGTGACCAGTGACTGGTATTATGCAAGAAGCCCC
  TTTCTGAAGCCAAAGCTGAGCTCTGACATTGTGGGTCAACTGTATGAGCTGACTGAGGTTCAGTTTGATC
  TAGCACCAAGAGGCTATGACTTGGATGCCGCCTGGCCAACATTTGCCAGGAGGACGCTAGCCACCAGCAC
  GTCTGCTTACATGTGGAAACCCCCCAGCCGAAGCTCCAGCATGAGTAGTTTAGTGAGCAACTACTTGCAG
  ACTCAGGAGATGGCCTCCAGTCTTGACCTGAATTGTTCTCTAAACAATGAAGCACTAGAAAGCTTCGACG
  AGATGCGGCTGGAGCTGGACCAGTTGGAGGTTCGGGAGAAACAGCTACAAGAACGTGTACAGCAGCTAGA
  CAGGGAGAACCAAGCACTGCGAATGTTGGTCAGCAGACAAGGGGGGCAGCTTCAGGTAGAGAAGGAGATG
  GGGTACCTTGCAGTCGAGGACAGCATTGGCCTTGTGAGCCTGGTAGCAGAACTCCAGAAGCAGGGGGACG
  TCAGCCAGGCCACAGTGAAGAAGCTACAGTCATGTCTGCAGGCCTTGGAGCTAAATGTAGACAAGAAAGA
  GTACAGTCCCTCAGCCTTGCAGCTTGAGAACATGGCGAAGGAGCTTGACACTGTGAGAGGCTCCTTGGGT
  AGGGAGAACCAGCTCCTGGCCAGCCTTTCAGAGCGCCTTGCTAGGGCAGAGAAAGGGGAAAAGACACCTC
  CAGACACAGAGCTTCATCAGGAGCCAGTTCCTGCGGATCTGGTGCTCAAGTTCCAGGAGCTGAAGGGAAA
  ACTTCAAGCCCTAGAAGGAGAGAACACCGAGGCCCAGGAGCTCAACAGGCAGCAGAGTATCAAGCTAGAG
  CAACTGGCCAAGGAACTACAGCTGAAGGAGGAGGCCCGGGCTAGCCTAGCACACTTAGTGAAGGATGTGG
  TCCCACTTCAGGAAGAGCTGTCTGGAAAGAAGCAAGAGTCAGCTCAGCTCCGACGACAGCTTCAGGAATC
  CCTGGCCCACTTGAGCTCTGTGGAGGAGGAGCTGGCTGAAGCCAGGCAGCAGGAAAAGCAGCATCGAGAA
  GAAAAGCAGCTACTGGAGCAGGAAGCCACGTCTCTTACATGGCAGCTGCAGCTCCTAGAGACCCAGCTAG
  GACAGGTGAGTCAGCTTGTAAGTGACCTGGAGGAGCAGAAGAAGCAACTCATGCAGGAGAGAGACCATCT
  CAGCCAGAGAGTGGGCACACTGGAGCAGCTAGCCGAGGTGCATGGCCCGCCACAGTCTGCGGAGATGCCG
  GAAAAGAGGCAGCAGTGCCTCCGGGAAGAACAGGTAAACAATAGCACAGTGTCAGAGGCAGAGCAGGAGG
  AGTTGCAGAAGGAGCTGCAGAACATGGTTGACCGCAACCAGCTCCTCGAAGGCAAACTGCAGGCCTTGCA
  AACTGACTATAAGGCACTGCAGCAGCGAGAGGCAGCAATCCAGGGCTCTTTAGCCTCCCTGGAAGCTGAA
  CAGGCTAGCATCCGGCACCTGGGAAACCAGATGGAAGCAAGCCTCCTGGCTGTGAAGAAAGCCAAAGAGA
  CTATGAAAGCCCAGGTGGCAGAGAAGGAGGCTGCCCTGCAGAGTAAGGAGAGTGAGTGCCAGAGGCTGCA
  GGAAGAGGCAGATCAGTGCCGGCTTCAGGCAGAGGCCCAGGCCCAGGAGCTCAGAGCTCTTGAGAACCAG
  TGCCAGCAGCAGATTCAGCTGATTGAGGTCCTCTCTGCAGAGAAAGGCCAACAGGGACTCAGCCTGCCCC
  AAGTCAACACAGACCAACTGGCCCTGTCTCAAGCACAGCTGGAAATCCACCAGGGGGAAGCCCAGCGGTT
  ACAGAATGAGGTGGTGGACCTCCAGGCCAAGCTCCAGGTGGCCCTAGGTGATCGAGACAAGTTACAGAGC
  CAGCTGGGTGTGGCTGAGACAGTTCTAAGGGAGCACAAGACCCTGGTGCAACAACTGAAAGAGCAGAATG
  AAGCCCTCAACAGGGCCCATGTTCAGGAGCTGCTTCAGTGCTCAGAGAGAGAGGGGATACTACAGGAGGA
  GAGCATCTACAAGGCCCAGAAGCAGGAACAAGAGCTACGAGCCCTGCAGGCAGAGCTGTCACAGGTGAGG
  TGCAGCTCTGAGGGAGCCCACCTGGAACATGCAGAGCTGCAAGACCAGCTGCACCGAGCCAACACAGACA
  CAGCTGAGCTTGGTATACAGGTCTGTGCACTGACAGCTGAGAAGGATCGAATGGAGGAGGCCCTGGCCAG
  CCTAGCCCAGGAGCTCCAGGACTCCAAAGAGGCAGCACTACAGGAACGAAAGGGCTTGGAGCTCCAAGTG
  ATGCAACTTCAGCAAGAGAAGGAGAAGTTGCAGGAAAAGGTGAAGGCAGCTGAGGAGGCAGCCAGTTCAT
  TCTCTGGTCTGCAGGCACAGCTGGCCCAGGCTGAGCAGCTAGCCCAGAGCCTCCAAGAGACTGCACACCA
  GGAACAAGATGCCCTCAAGTTCCAACTAAGTGCTGAGATTATGGACCACCAGAACCGATTAAAGACAGCC
  AATGAAGAGTGTGGGCACCTCAGGGCCCAGCTAGAAGAACAAGGCCAGCAGCTGCAAATGACTAAGGAGG
  CTGTGCAGGAACTGGAGATCACCAAGGCGGCCATGGAGGAGAAGCTGAATTGCACCAGTAGCCACCTTGC
  AGAGTGCCAGGCCACTTTACTGCGCAAAGATGAAGAAAGCACTATGCTTCAAACCAGTCTAGAAAGAACA
  CAGAAGGAACTTGAAAAGGCTACATCAAAAATTCAAGAATATTACAACAAACTCTGCCAAGAGGTGACAA
  ACAGGGAAAGGAATGACCAGAAGATGCTTGCAGACCTCGATGACCTGAACAGAACCAAGAAATACCTTGA
  GGAGCGGCTGATAGAGCTGCTCAGGGACAAAGATGCTCTCTGGCAAAAATCCGATGCACTGGAATTCCAG
  CAGAAGCTCAGTGCTGAAGAGAAATGTCTTGGGGACATGGAAGTCAACCACTGCCATGACTGCAAGCGGG
  AGTTCAGCTGGATAGTGCGACGGCACCACTGCAGGATATGTGGCCGTATCTTCTGTTACTACTGCTGTAA
  TAACTATGTTGTGACTAAGCCCAGTGGCAAGAAGGAACGCTGCTGCCGAGCCTGCTTCCAGAAGTTCGGC
  GAAGGCTCTGGATCCAATGATAGCAGTGGTTCAGGCACTAGCCAGGGAGAGCCCAGCCCCATGGTGTCAC
  CAGCTGAAGCAAGTCCCCAGTCCATAGGAAGCCAAGGGATAAACTCAGTCTGCAGACCACCAGACGATGC
  TGTGTTTGACATCATCACTGATGAAGAACTGTGCCAGATCCAAGAATCTGGCTCCTCCTTGCCTGAAACA
  CCCACTGAAACTGATTCAATGGACCCGAATACGGCTGAACAGGACACCACATCAAACTCATTAACCCCTG
  AAGACACTGAAGACGTGCCCATGGGGCAAGATGCTGAAATCTGCTTGCTGAAGTCAGGAGAGCTGATGAT
  CAAATTACCCCTCACAGTAGAGGAGGTCGCCAGCTTCGGGGAGGGCAGCAGGGAGCTGTTTGTGAGGTCC
  AGTACCTACAGCCTGATCACCATCACCGTGGCTGAGCCTGGCCTCACCATCAGCTGGGTCTTCTCTTCTG
  ATCCCAAGAGCATCTCCTTCAGTGTGGTCTTCCAAGAGACAGAAGACACGCCGCTCGACCAGTGCAAGGT
  CCTCATTCCCACCACCCGATGCAATTCCCACAAGGAGAACATCCGGGGCCAGCTGAAGGTCCGAATCCCT
  GGCATCTACTTGCTCATCTTTGACAACACCTTTTCAAGATTTATCTCCAAAAAAGTGCTTTACCACCTGA
  CTGTTGACCGGCCTGTGATCTACGATGGAAGCGATTTCCCATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001110253
• Insert Size: 4314 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001110253.2, NP_001103723.2
• RefSeq Size: 7702 bp
• RefSeq ORF: 4314 bp
• Locus ID: 17281
• UniProt ID: Q8VDC1
• Cytogenetics: 9 74.52 cM
• Gene Summary: May mediate microtubule plus end-directed vesicle transport.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Both variants 1 and 2 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. CCDS Note: The coding region has been updated to remove a 3-nt insertion versus the reference genome sequence, supported by the available transcript data.