Hfm1 (NM_177873) Mouse Untagged Clone

CAT#: MC224461

Hfm1 (untagged) - Mouse HFM1, ATP-dependent DNA helicase homolog (S. cerevisiae) (Hfm1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Hfm1
• Synonyms: A330009G12Rik; Gm1046; Mer3; Sec63d1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224461 representing NM_177873
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAAAGTCAGACGATTGCTTCTTTTCCATGGATAATTTGTTTTTCAGTAGCCCAGATGAAACTGAAA
  ACTTTCCAGTTAAGGAAAAATCACTGGATTGGTTTCTCCCTCCTGCTCCACTGATTTCAGAAATTCCAGA
  TATTCAAGAGTTAGAGGAAGAAATTGAAAGTTATAAGCTACTAGGGAAAGGAAAGATGCCAAGAATGTTA
  ACATCAAACTTGAAGATCATTAATGAAGACACAAATTGTATTTCACCAACACAAAAAATCCATTTTTCCT
  ATAATGTACATGAACAGGATTACCTAAATTTAGGAGGATCAAATAACAATGACATGTCCCATGTAGCTGG
  GAAGCTGATGTATGGTTCTTCTCAGAAATATAAAAATCACATGGGTGCTAAGAGTCCATCTGCAAGGAGT
  AGTCCTGGTGATACAAAATTACATGATGTGGCAGAAGACAGACAGGGCACATCAGCATTCAAAAAAAGAC
  TGTCTAGAACATGTGACAGTGAACATGACTGTGACTATGCTGATGGAAGTTTAAACTTGAGTTCTCATAT
  CAGCCCAGTGAAACTTACCCAAACGAAAATAAGCAAAGAGAATGCATGGACGTGTAGCAATAGTAAGCAG
  AAACGTCAGTATTCTACAAACAAATTCAAAGCAAATGATGCTTTTTCTGCTTCTGGAATTGGAAAAGACA
  TATTCAAAGCACCATCATTTCCTGCTGCATCTCAGCCTCATGATATTCAAGGAATAACCCCAAATGGCTT
  AGGTTCTTTGAAAGCTGTCACGGAAATCCCGGCAAAATTTAGAAATATATTCAAAGAATTCCCTTACTTC
  AACTATATACAATCCAAAGCCTTTGATGATCTTCTTTACACAGATAGGAATTTTGTGATTTGTGCCCCCA
  CTGGTTCTGGGAAAACTGTAGTGTTTGAGCTAGCGATAACAAGATTGTTAATGGAAGTACCATTGCCATG
  GCTAAACATGAAGATTGTTTACATGGCACCAATAAAAGCCCTTTGTAGTCAGCGGTTTGATGACTGGAAA
  GAAAAGTTTGGACCAGTAGGCTTGAATTGTAAAGAACTTACTGGTGATACAGTAATGGATGACCTGTTTG
  AGATTCAGCATGCAAATATAATTATAACAACTCCAGAGAAGTGGGACAGTGTGACTAGGAAGTGGAGAGA
  CAACTCTTTTATCCAACTGGTTCGACTGTTTCTCATTGATGAGGTGCATGTTATTAAAGATGAAAATCGT
  GGTCCAACTCTTGAAGTTGTAGTTAGCAGAATGAAAACTGTACAGTCTTTATCTAGGGATTTAGAAAGTG
  CCAGCCCTGTTCCAGTGAGATTTGTAGCTGTGTCTGCAACAATTCCCAATGCTGAGGATATCGCAGAATG
  GCTTTCAGATGGTGAGAGACCTGCTGTATGTCTCAAAATGGATGAGAGCCATAGACCTGTGAAACTTCAG
  AAAGTAGTCCTGGGTTTTCCCTGTAGTAGTAGCCAAACTGAATTTAAGTTTGATTTGGCCCTCAACTATA
  AAGTGTACAGTGTTATTCGAACATACTCCGATCAGAAGCCCACACTTGTGTTTTGTTCAACAAGGAAAGG
  TGTGCAGCAGGCTGCTTCTGTTCTTGTGAAGGATGCTAAGTTTATCATCTCTGTGGAGCAGAAACTACGG
  TTGCAGAAGTCTGCATATTCTATAAGGGATTCAAAGCTGAAAGATACCTTGGTATATGGTGTTGGTTACC
  ATCATGCTGGAATGGAGCTGTCAGATAGAAAATTGGTTGAAGGATTGTTTACCTCTGGAGATCTACCAGT
  TCTTTTCACTACCAGTACTCTAGCTATGGGCATGAACATGCCGGCTCACCTGGTTGTTATAAAGTCTACC
  ATGCACTACAGTGGAGGTGTGTTTGAAGAGTACAGCGAGACCGACATCCTGCAGATGATTGGGAGAGCTG
  GCAGACCCCAGTTTGACACTACAGCTACTGCAGTCATTATGACTCGACTAAGTACAAGAGAGAAGTACGT
  GCAGATGCTAGCTTGTAACGACACTGTAGAGAGCAGTTTGCACAGACATCTTATTGAACATTTAAATGCA
  GAAATAGTGCTGCATACCATCACAGACGTGAACATTGCTCTGGACTGGATCCGATCAACAATGCTGTATA
  TCAGAGCTCTGAAAAACCCATCTCACTATGGTTTTTCATCTGGATTGAACAAAGATGGGATTGAAGCAAA
  ATTACAAGAATTATGTTTGAAGAATCTGAAAGATTTATCATCACTAGACCTGATAAAGATGGATGAAGAT
  GTTAATTTTAAACCAACAGAGGCAGGAAGACTAATGGCTTGGTATTATATTACATTTGAGACAGTCAAGA
  AATTTTGTGCAATCAGTGGAAAAGAAACTTTATTGGATTTAATTTCAATGATATCGAGCTGCAATGAGTT
  TCTAGATGTGCAGCTGAGGATAAGTGAAAAGAGAATACTGAACACCTTAAACAAAGACCCAAACCGGATA
  ACTATCAGATTTCCAATGGCAGAAAGAATTAAAACAAGAGAAATGAAAGTAAACTGTCTTATTCAGGCTC
  AGCTAGGATGCATTCCTATACAAGATTTTGCTTTGACACAAGATACAGTGAAGATTTTCAGAAATGGCTC
  ACGAATTGCAAGATGGTTGTCAGATTTTGTGGCTGCTCAGGAAAAGAAGTTTGCTGTGCTTTTAAACAGT
  GTGATTTTAACTAAATGTTTTAAGTGTAAACTCTGGGAAAACTCTAAACATGTATCAAAACAGCTGGATA
  AAATTGGCATATCATTGTCAAATACCATGGTAAATGCAGGCTTAACTTCTTTTAAAAAAATAGAAGAAGC
  AAATGCAAGAGAACTTGAACTGATTTTAAATAGACACCCACCATTTGGGACCCAGATAAAAGAAGCTGTG
  GCGCATCTGCCGAAGTATGAGCTTGAGGTGGAGCAGATTGCAAGATACAGTGATATCAAGGCAGAAATAC
  TGGTGACCATTATATTAAGAAACTTTGAGCAGCTGCAAACTAAAAGAACAGCACCAGACTTCCACTATGC
  CACCTTAATCATAGGTGATGCAGATAACCAAGTCGTTTTTAAGCACAAAATTATGGATTCTGTCTTGTTA
  AAAAGTGGAAATTGGGTTAAAAAAATTGATGTGAAGAGAGCTCTTATATCAGAAGATCTCAGCATAAATC
  TAATTAGTTCTGATTATGTTGGTCTTGATATTCACCAGAAATTTACAGTCTTTTATTTCGGACCCAGGAA
  GTTTGTAAATGAAACAGCTATGGAAAGAAGCTCAGAAACAGATATTTCTCATTCTGACTACTCAGGTAGA
  GCCACTGCAACAGGATCCAGTAAAGGAATGGCTACCTGCAAAAAACCTGGGAACAGAGAATGCCATCATC
  ACTGTAAAAATAAACACGCATGTGGACATGACTGCTGTAAAATTGGAGTTGCACAAAAGCCGGAAGTTAA
  AGAGTCAGCAATGTCTTCATATCTGTCTGACTTAAAAAGCAGAGATGCTGTTTCTTCTCTTCCTCTAGCC
  AAGCGACTCAAGATACAGATGAACAAATCTCAAAATGTGGACCTTAAAGAGTTTGGTTTTACTCCAAGGC
  CTTCCCTTTCCAGTATATCAAGGTCAGAATATTTAAATACCCCTGAATTGTCAATATTAGAACAGAGGAA
  TCAGCATGAAATCTATGGAAAAGTTCAACAGGGACCATCTGAATATCGTGACAAAGAAGTTCTGGGTGTG
  AACTTAGAGCTGGGAAATGAAGTCTGGGATGACTTTGATGATGAGAGCTTAATAGAAGTTATGAGCCTTT
  CAGCTGATGCTGAGAAGATGGCTGCATCAGGATTTGGAGACACTCGTGATTCAAGTCTCGGCGGAAGTAA
  GCTACCCTTCCAAAAGTCAAGCAGCAGATTCCAAAGAGACAACTCAAACAGTTTTGCTTCGTCGCCTGGG
  AAGCCAGATGCTTATTTGCGAGATTGTTCCAGGTCCAGCTTCGGGCTGTCCTCTGTGGCAGAGATCCCTC
  AGCGGGCTGAGAATGCAAGTCTTGCCAATTTACAAGAGAGGAGACCGCTGACTCTCTCACCAGTGATTGA
  GAGGATGTGCTTTGCCCACTCTAAGAAAACACCACAGTCTCCCAAGTTTAAGGAAGTGGATATTTTTATT
  GGAAACAGTGGCTCTAAAAAGGAAATTGATCTTAGTAAGTATTATCCTGATGATGCAGCTGAAGAAATGA
  AGGCTCTTCTGGGAATATTTAATGGCATTTTCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_177873
• Insert Size: 4305 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177873.3, NP_808541.2
• RefSeq Size: 5018 bp
• RefSeq ORF: 4305 bp
• Locus ID: 330149
• UniProt ID: D3Z4R1
• Cytogenetics: 5 E5
• Gene Summary: Required for crossover formation and complete synapsis of homologous chromosomes during meiosis.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.