Vps8 (NM_001081366) Mouse Untagged Clone

CAT#: MC224457

Vps8 (untagged) - Mouse vacuolar protein sorting 8 homolog (S. cerevisiae) (Vps8), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Vps8
• Synonyms: AI315068; AU040738; mKIAA0804
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224457 representing NM_001081366
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAACTGAACCAGACCAGGAGCATTTGGACCAGAACCCGTGCGCCAGGACTGTGGAAGAAGAGTTGA
  GCAAGTCTTTTAATTTAGAAGCTTCCCTTTCAAAATTCTCTTGCCTAGATCTGGATAAGGAGCTGGAGTT
  CAGAAGTGACCTGATCGATGACAAGGAGTTTGATATTCCTCAGGTGGATACCCCTCCAACTCTGGAAAGC
  ATTCTTAATGAGACGGACGATGAAGATGAGTCTTTCGTTCTGGAGGACCCTACCTTGTTAAACGTTGACA
  CCATCGACTCTCACTCTTACGATACCTCATCTGTGGCGAGCTCAGACAGCGGCGACAGGGCCAACCTAAA
  GAGGAAGAAGAAGTTGCCGGACTCTTTCTCGCTGCATGGGTCGGTTATGCGACATTCACTTCTAAAGGGG
  ATATCTGCACAGATCGTGTCCGCAGCCGACAAAGTAGATGCTGGCTTGCCTACAGCAATTGCCGTGTCCA
  GTCTGATAGCGGTGGGAACGTCTCACGGACTGGCTTTAATATTTGGAAAAGATCAGAATCAAGCACTGCG
  GCTCTGCCTGGGTAGCACCAGTGTTGGAGGGCAGTATGGAGCCATCTCTGCCCTCAGCATCAACAATGAC
  TGCTCAAGACTTCTTTGTGGCTTTGCTAAAGGGCAGATCACCATGTGGGATTTGGCCAGTGGAAAACTTC
  TCAGATCAATAACAGATGCTCATCCTCCAGGGACCGCAATCTTGCACATCAAGTTCACAGATGATCCAAC
  TCTTGCGATTTGCAACGACAGTGGAGGCTCTGTTTTTGAACTAACATTTAAGAGAGTGATGGGCGTGCGA
  ACGTGTGAGTCTCGGTGCCTATTCAGCGGCTCCAAGGGTGAGGTCTGCTGCATCGAGCCGCTGCACTCAA
  AGCCCGAGCTGAAAGACCATCCCATCACGCAGTTCTCACTGCTGGCCATGGCGTCCTTAACAAAGATATT
  GGTCATTGGGTTGAAACCATCCCTGAAAGTGTGGATGACTTTCCCCTATGGACGGATGGATCCTTCCAGT
  GTTCCGCTGCTGGCCTGGCACTTCGTGGCAGTGAATAACTCTGTGAATCCCATGCTCGCCTTCTGCAGAG
  GCGACATGGTTCACTTTCTCCTGGTTAAGAGAGATGAGTCGGGCGCAATACACGTCACCAAGCAAAAGCA
  CCTTCACCTGTACTATGACCTGATCAACTTCACTTGGATCAACTCGCGCACAGTCGTGCTTTTAGACAGC
  GTGGAGAAGCTGCACGTGATTGATCGGCAGACGCAGGAGGAACTGGAGACAATGGAGATCTCGGAAGTCC
  AGCTGGTCTACAACAGCAGCCACTTCAAGTCATTGGCCACGGGAGGAAATGTTAGCCAGGCCCTGGCTTT
  GGTTGGAGAGAAGGCTTGCTACCAATCCATCAGTAGCTATGGAGGACAGATCTTCTATCTGGGGACCAAG
  TCTGTGTACGTGATGATGCTGAGAAGCTGGAGGGAGAGGATGGACCATCTCCTGAAACAGGATTGCCTTA
  CGGAAGCCTTGGCCCTCGCGTGGTCTTTTCATGAAGGAAAGGCAAAAGCAGTAGTGGGGCTATCGGGGGA
  TGTCAGCAAGAGGAAGGCTGTTGTTGCTGATCGGATGGTAGAGATCCTGTTCCATTATGCAGATCGGGCT
  CTGAAAAAGTGCCCGGACCAGGGGAAAATCCAAGTGATGGAGCAACACTTTCAGGATACGGTGCCAGTCA
  TAGTGGACTACTGCCTTCTGCTCCAGCGAAAGGATCTTCTATTTGGTCAAATGTACGACAAATTAAGTGA
  AAATTCCGTCGCAAAAGGCGTGTTTTTGGAGTGCCTTGAGCCATATATCTTAAGTGATAAACTGGTGGGG
  ATTACTCCCCAGGTCATGAAGGACTTAATTGTTCATTTCCAAGATAAAAAACTGCTGGAGAATGTGGAAG
  CCCTCATTGTCCACATGGACATCACCAGCCTCGACATTCAGCAGGTGGTCCTCATGTGTTGGGAGAATCG
  TCTGTATGACGCCATGGTCTATGTCTACAACCGAGGCATGAATGAGTTTATCAGTCCGATGGAGAAGCTT
  TTCAAAGTCATCGCCCCACCTCTGAATGCAGGGAAGACTCTTACAGATGAGCAAGTTGTTATGGGCAACA
  AGCTTCTGGTGTACATCAGCTGTTGCCTGGCAGGTCGTGCCTACCCTCTCGGTGACATTCCTGAAGATCT
  TGTTCCCTTGGTTAAAAACCAGGTTTTTGAGTTTCTGATTCGCCTGCATTCTGTAGAAGCATCCTCCGAG
  GAAGAAGTCTATCCTTATGTTCGGACTTTGCTACATTTTGACACAAGAGAATTTCTGAATGTGCTGGCAC
  TGACCTTTGAAGACTTTAAAAATGATAAGCAAGCTGTGGAATACCAGCAGCGCATTGTGGATATTTTATT
  GAAGGTTATGGTGGAGAATTCAGATTTTACCCCGTCACAAGTGGGGTGTCTCTTTACGTTCCTCGCTCGT
  CAGCTCGCAAAGCCTGACAATACCTTGTTTGTGAACAGGACCCTCTTTGATCAAGTCCTCGAGTTCCTCT
  GTAGCCCCGACGATGACTCCCGCCACTCTGAAAGACAGCAGGTCCTTTTAGAATTGCTGCAGGCCGGAGG
  CATAGTTCAATTTGAAGAGAGTCGGCTCATCCGCATGGCAGAAAAGGCAGAATTCTATCAAATCTGTGAA
  TTTATGTATGAGCGGGAACACCAGTATGACAAGATCATTGACTGCTACCTGCACGACCCATTGAGAGAGG
  AAGAAGTCTTCAATTATATCCACAACATCTTATCTATTCCTGGTCACAGTGCTGAGGAGAAGCAGTCTGT
  GTGGCAGAAAGCAATGAATCATATGGAGGAACTTGTGTCCCTGAAGCCCTGCAAAGCCGCAGAGCTGGTG
  GCTACTCACTTTTCTGAGCAGATCGAAGTGGTCATTGGACAGCTTCAGAACCAGCTTTTGCTTTTCAAAT
  TTTTGAGGAGTCTTCTTGATCCAAGGGAAGGTGTTCATGTCAATCAGGAGTTGCTGCAGATCCCGCCTCA
  CATTACAGAGCAGTTCATTGAGCTGCTGTGCCAGTTCAGTCCTGACCAAGTCATACAGACACTGCAGGTC
  CTTGAGTGCTACCGTCTGGAGGAAACGATTCAGATTACACAAAAGTATCAACTCCATGAAGTCACTGCGT
  ATCTGCTGGAGAAGAAAGGGGATGCACACGGCGCCTTCCTGCTGCTGCTGGAGAGACTGCAGAGCAGGCT
  GCAAGAGATGACACGGCAGGACGAAAATACCAAGGAGGACATCCTGCTGAAAGGTGTTGAAGATACCATG
  GTAGAGACAATTGCTCTTTGCCAGAGAAATTCACAGAATTTGAATCAGCAGCAACGAGAGGCTCTATGGT
  TTCCACTGTTGGAGGCAATGATGACACCACAGAAGCTGTCCAGCTCGGCTGCTGCTCCTCATCCGCACTG
  TGAAGCTCTGAAGTCTTTGACCATGCAAGTCCTAAACAGCATGGCAGCGTTCATCGCCCTCCCGTCCATC
  CTACAGAGAATCTTGCAGGACCCAATTTATGGAAAAGGAAAGCTTGGAGAAATCCAGGGCCTTATTCTGG
  GGATGCTGGACACCTTCAACTATGAGCAAACCTTGCTAGAAACAACAGCCAGCCTCCTGAACCAAGATCT
  CCATTGGTCACTGTGTAACCTGAGAGCATCAGTATCCAGAGGACTCAATCCCAAGCAGGATTACTGTTCT
  ATATGTTTACAGCAGTACAAAAGACGCCAAGAAATGGCTGATGAAATTATTGTCTTTAGCTGTGGCCATT
  TGTATCATTCATTCTGTCTCCAAAGTAAGGAATGCACCCTAGAGGTTGAGGGTCAGACGAGATGGGCGTG
  TCACAAATGCAGCTCAAGTAATAAAGCGGGCAAACTCAGTGAAAATCCTTCTGAAAACAAGAAAGGACGG
  ATAACCTCGTCTCAGGTAAAAATGTCGCCCTCGTATCATCAGTCCAAAGGGGATCCTCCCGCCAGGAAGG
  CAAACTCAGAACCTGTTCTGGACCCACAGCAAATGCAAGCCTTTGATCAGCTCTGCCGTCTCTACAGAGG
  AAGTTCTAGGTTGGCTCTCCTTACGGAGCTCTCCCAGAACCGAGGTGGCGACAGCTGCAGGCCGTTTGCT
  GGCCCCCAGAGTGGGCCTGCTTTCAACAGCGTCTTCCAGAAGGAGAACTTCCAGCTGCAGCTCGCGCCTC
  CGCCTGTGGCTGAAGACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001081366
• Insert Size: 4290 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081366.1, NP_001074835.1
• RefSeq Size: 5032 bp
• RefSeq ORF: 4290 bp
• Locus ID: 209018
• Cytogenetics: 16 B1
• Gene Summary: Plays a role in vesicle-mediated protein trafficking of the endocytic membrane transport pathway. Believed to act as a component of the putative CORVET endosomal tethering complexes which is proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations. Functions predominantly in APPL1-containing endosomes (By similarity).[UniProtKB/Swiss-Prot Function]