2700049A03Rik (NM_029818) Mouse Untagged Clone

CAT#: MC224455

2700049A03Rik (untagged) - Mouse RIKEN cDNA 2700049A03 gene (2700049A03Rik), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: 2700049A03Rik
• Synonyms: mKIAA0586; Ta3; Talpid3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224455 representing NM_029818
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAAACGTTGAGTTTAGCCTGGAAAGGGGGCAAAGGCTTAAAATGCCAGCGAGGAAACTGCGAGAGA
  TTGTTTCTCCAAACCAAGGGAATAAATTGGCTGTGGTGGAAGATGAGCTGCCCCGTGTTCCTCCAGCCCT
  GGCTGCAAATAAACGTCTTGCTGTTGAAACGAGGACTAGTTCTAATGGAACGTTATGTGGCTCCTTGGAT
  TTAACTTCTGCAAGACTGTATCACCAGCCTCTGTTAGAAAGTCCTCCTGCGTCCAAGAAAAGTGATTTTT
  CTAAAGATGCTGTAGTTCGGCAGTTACCTTTGAATAAAACAGAAGAGAACAACGCACCAAAAGCAAATGA
  CATCTTTATTTCTCAGTACACCATGGGACAGAAAGATGCTCTGAGAACTGTTTTAAAGCAAAAAGCTCAA
  AGCATGCCTGTTTTTAAGGCGGTGAAAGTACATCTTTTTGAAGACACGAGTACAGAAAAGAATACTGTTG
  CTCAGGAGACTGAAACTCCACCCAATAGAATTGATTCAGCTACAACTGTGGCTGCGGCAACTGCCGCTGC
  CATCGCAACAGCAGCTCCTTTGATCAAGGTACAGAGTGATTTGGAGGCAAAGGTTAATTGTGTTGGAGAG
  TTACTTACTAAGTTACAGGAGACTGATAAACAGTTACAGCGCGTGACAGAGCACCAAGCCAGCGTTCAGA
  GTAAGCAAGAGAAAGTGCACTGTCATGACCACGACAAGCAGATGAACGCGTTCATGGAGCAGCATATCAG
  GCACCTTGAAAAACTACAGCAACAGCAGATAGACATTCAGACCCATTTTATTGATGCTGCACTCAAGGCT
  AGCAGTCTTCAACTTGGCATGTCCACGTCCAGAGCAGTGGGGAAGTATTCTGGAAAACTGGGAAGCCCTA
  GTGTTGGGAGCAGTGTTTTTTCACACAACACATTTGTTTCTAAACGAGTGCCTTTAAGTGAAGACACAGA
  TTTTGATGGGCAGAAATCTCCTTTAGAGACCCCAGCACCTCGCAGGTTTGCCCCTGTACCTGTTTCAAGG
  GATGGAAAAATAACAAAAAGGGAAAGCCCTACAGAAGAAAAAGAAAATATGGAAATGAACAGTCCTAAAG
  GAAATGTAAGACTGTTGGAACAAGTACTGAATAGTAATGAGTGTTTGACAAGAAAGACTGAATCATCAGA
  TATAACCTCACTAACTCAGCCAAAAATGGGATGGAATTTAGAGAAAAGAGACAGCACTGAAACTCTACAT
  TCACAAATATTTCCTTCCTCTGAAGAGCGGGGAACAGCTCAAGTACCCGTGCCGAAGTATAATGATGTTG
  TTCATGATCTTGGCCAGAAGAAACAAGCAAGTGACATGCTCCAGATAAAGCAATCTCCAGTTACCTTGAG
  GCTTTCAGATCATCCGCATAATCCTGCCTTGCTCCAGACAACCAATACAAGGTCTGTCTTGAAAGATGCT
  GCGAAGATTCTGAGAGGAGTACAAAACAATAAAAAAGTACTAGAAGAAAACCTGGAAGCTATCGTTCGTG
  CAAAGGATGGAGCTGCTATGTATTCATTTATCAATGCTTTAGCTACCAATAGAGAGATGTCAGAGAAAAT
  TAGGATCAGAAAGCAGGTAGATGAATGGATTAAAATCATTTCAGCAGAAATCCAGGATGAACTTATGAGA
  AAAGATTATGAACAAAAGAGATTTGATCCAAAGAATCAAAGAAACAAGAAAGCTCTAACTATGAGTAGAG
  ATATTAAAGCCAATAACCAAGAAAAAACGGTGAACAGATCTGTAATTCCAAGAAGCCATTATCAAAAGCA
  AACCCAGGAGCAGTTTACAAGTCCACCTGTGAGGAACTTGCCTGCCTCCGGCCCGCAGAAAGAGAGGAGT
  GGCCTCTTGAAGTCAGCCACAACGTTGCAAGACGAGGATTATATGTTACAGATATATGGGAAGCCAGTTT
  ATCAGGGCCACCGCAGCACTCTTAAGAAAGGACCGTATCTCAGATTCAGTTCTCCGTCTCCTAAGGCCAA
  GCCACAGAGGCCAAGGGTGATAGAGCTAGTGAAAGGCACTAAAGTCAAGTCAGCAAAAACACAAACTGAC
  TTTCATGCTGCATCACGTATGAAAATGGATTCTAAAATACAGCATCCCATCACCGCACTACCTCATGCTG
  ATCAGCAGTATATGGTCAGCCCAAGCAGAGAAATGCCTACTGTTTCAGGTACCCTGGAAGGTCATCTAAT
  TCCTATGGCAATCCTCTTAGGACAAACCCAAAGCAATAGTGACTCCATGCCACCTGCTGGAGTCACTGTA
  AACAAGCCACGCCCCGTGACTGTGACCACTTCTATTCCTCCGGCATCTCGAAAAGGGAACGCTGGAGTCA
  AGAAACCCAACGTAGCCATTGTAGAGATGAAATCAGAGAAGAAGGACCCTCCCCAGCTCTCTGTACAGAT
  ATTACCTAGTGTGGATATTGATAGCGTTTCATACAGCAGTACTGACGGGGCTTCCTCTCCACCAAGTCCC
  AAAGAAGCCTCTCTTCCTCCACTGCACACCTGGATCCAGACTCCAGACTTTATGAAGGTGGATGAAGAAG
  AGGTGCCCCTTCCAGGAACTAACTTTGATGAAGTAATCGATGTTATTCAGGAAGAAGAAAAACGTGATGA
  AATTCCTGAATGCTCTGCACCAATGTTGGAGTTTAACAGAAGTGTTAAAGTTGTTCCTACAAAATATAAT
  GGTCCTTCATTTCCTCCTGTAGTTTCTGCTTACCATCCCACTACTGATATTTTAGATAAAGTAATTGAGA
  GAAAAGAAACATTGGAAAATAGTTTAATTCAGTGGGTAGAACAAGAAATAATGTCAAGAATTATCTCTGG
  GCTCTTTCCACTCCAGCAACAGGCCAGACTTGATGCCAGTGTCTCAGTAAGTGAGGCGAGCGAACCGTCA
  GCTTCTGACATTGTTGCAGGAACAAGCAGTGGAGCGCTCCAGCGTATGGTTGATGCCAGAGTTCCTGTGA
  ACTCGGACATGGTCAGCCATTTTGTCAATGAAGCTCTGACTGAGACCATTGCTGTCATGTTAGCTGACAG
  AGAAGCAGAGAGGCAAAGGGCTGCTGCCACAAGTGTTCCTGGTGATCTTTCAGGAACTGAAACTAACCTG
  TTGGCAAGAGTTTGTGCTCCAGTAGCGACGCCGCAGCCCACTCCCCCCTGCTCACCATCACCTGTGAGGG
  AGCACGTGCGAGTGAAAACGCCAGACTCTTCTCCCTGTGAATCCGATCCAGATGCGGCTTCTTCTATAAA
  AGAGATACGCGTTGAAAAAGGAAGTGATATGCCTGCTGTCATGCTTGTTAGTACACCAACACGCACTCCT
  GTCGCTACCCCTCCTCCAGCAGCAGCTCTTACCCCAACTTTGTCCGAAACTTCCATTGACAAGTTGAAGT
  TATCAAGCCCTGAACTTCCCAAGCCATGGGACAGTGGAGACCTGCCACTGGATGAAGAGAATCCTAACTC
  TCTTCAAGAGCTTCCTCACCCAAGAGCGGTTGTAATGTCTGTGGCTAATGAAGAACCTGAGAGTGTGGAC
  TTTTCTGCTCAGCCTGCACCTCCGGAGCCAGCTCCCTCAGCACCACTTCCTGAGGGCACCAAGGCCCCTT
  CCCTCCAGCGAGTACCAAGTTCAGGTTCATCCACCCTGGAGAACACTCTGAGCACTGTCACTGAAACAGA
  AACGCTAGACAGGCATATATCTGAAGGGGAGATCTTATTTAGCTGTGGTCAAAACTTGGCTACCAAGAGA
  CCTGGGGACTTGTTTCTGATGAACATAAATGACAGCTTATCCAGCACTCTGCAGGATGCCCTTGAAATGG
  AAGATGACCCTCCCAGTGAAGGGCAGGTGATTAGGAGGCCCCACAAAAAGCGTCATGAAGATGCCATCGT
  TGCCCTTCTTACTAAGCAACAACGGGAGTTATTAGTTTCACAGCAAGAGGAGGACTTGGATAACAGCGTG
  GGTGAGCTTAGTGAAGGACAGAGGCTCGTGCTGAAAGCAGCAGAGGACATCTCAGCAGGACCGTCCGGCC
  AAATGCTGCCGCCCACTTCTCCTGCAGAGCCCTCCTATCAGCACGCAGACCCTCGGCTGGTATTGCAGCA
  GTCTGACATGGCCTCAGGTGGCCCGGCAGATCTGCTCCTCATTGCACATGTGTCACCCGCCAGGATGTCT
  GTGACTCTACCCTCTGCGAACCTTGAGGACTGCTCTCAGTCCCTGAGCACCAGCTCCATGCATGGCGGCA
  CCGAATCCTCAGGGACAGACACCTTCTGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_029818
• Insert Size: 4299 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029818.1, NP_084094.1
• RefSeq Size: 5499 bp
• RefSeq ORF: 4299 bp
• Locus ID: 76967
• Cytogenetics: 12 C2- C3
• Gene Summary: Required for ciliogenesis and sonic hedgehog/SHH signaling (PubMed:21750036). Required for the centrosomal recruitment of RAB8A and for the targeting of centriole satellite proteins to centrosomes such as of PCM1. May play a role in early ciliogenesis in the disappearance of centriolar satellites that preceeds ciliary vesicle formation (By similarity). Involved in regulation of cell intracellular organization (PubMed:26386247). Involved in regulation of cell polarity (By similarity). Required for asymmetrical localization of CEP120 to daughter centrioles (PubMed:25251415).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks two in-frame exons in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 2) compared to isoform 1.