Adamts20 (NM_001164785) Mouse Untagged Clone

CAT#: MC224437

Adamts20 (untagged) - Mouse a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 20 (Adamts20), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Adamts20
• Synonyms: Adamst20; ADAMTS; bt
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224437 representing NM_001164785
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGGTGGCCAAGTGGCTGACCGGGTTGCTCTGCCCGATCTCGCTCCTTCTCACTGGGTCCTGGGAAG
  TTCGTTTCCACCCACGACAGGAAGCCCTGGTGAAGACTCTGGCCTCCTACGAAGTGGTGACCCCCACGCG
  GGTCAATGAGTTCGGAGACGTGTTTCCCCAGAACCGGCATTTCAGCCGGAAGAAACGTAGCTCCGGGGTG
  CCGGAGCCTCCTCCGTTCAGGACCCACTACCGAATCAGCGCCTACGGGCAGCTCTTCCAGCTGAACCTGA
  GCGCTGATGCGGCCTTCCTGGCTGCAGGCTACACTGAGGTGCACTTGGGGACCCCGGTACCTGGACCAGG
  CGGGCGCAGCACGGAGTCCCCAGACCTGCGCCACTGTTTCTACCGTGGCCAGGTCAACGCGCGAGAGGAC
  CACACTGCTGTCTTCAGTCTCTGTGGAGGACTGATGGGGACATTCAAAGCAAATGATGGGGAGTATTTCT
  TAGAACCAGTCCTGAGGGCCGATGGGAGTGCGCATGACGATGACCACAACAAGCCACATCTGATCTACAG
  ACAGGAATTAAAGAGGAACTCCTTTGCGCGGTCTCACAAGCCTTGTGAAGTTTCAGAAAATCAAATGGAG
  AAAACTGCTTTGCCCTCTCAGAGCTCCAGAAACACGACTGGAGATGTTGATATCGAAGAGGAAGCGGTGT
  TCAGATTGGAAGGCGAACGGAGCCAGTTACATTCCAGGAATAAGCGTTTTTTATCATACCCAAGATACGT
  GGAAGTTATGGTTACAGCCGATGCTAAGATGGTTCACCATCATGGGCAGAATTTGCAGCACTATGTTCTG
  ACACTGATGTCGATTGTTGCTGCAATCTACAAGGATTCCAGCATTGGGAACCTTATCAATATAGTGATTG
  TGAAATTAGTTGTGATTCACAGTGAACAGGAAGGACCAGTGATCAGTTTTAACGCAGCCACCACGTTACG
  CAACTTTTGCTTATGGCAACAGAGTCAGAATGTCCCGGATGATGCTCACCCCTCCCACCATGACACTGCT
  GTCCTCATCACTAGGGAAGACATTTGCGGAGCTAAAGAGAAATGCGACACGTTAGGCTTAGCAGAGCTAG
  GCACCCTGTGTGATCCTTCACGGAGCTGCTCCATTAGCGAAGAGAACGGGCTGAGCGCCGCCTTCACCAT
  CGCCCATGAGCTTGGACACGTGTTTAATGTTCCACACGACGACAGCTTTAAATGTAAAGAAGCCGGAATT
  AAACATCAGTATCACGTCATGGCCCCGACTCTAAATTACCACACGAGTCCTTGGACCTGGTCAGCATGCA
  GTCAGAAGCATATCACAGAATTTCTAGACACTGGTCATGGAGAATGCCTTCTTGACAAACCCAACGGGAG
  AACATATGACCTGTCTCCACAGCTTCCGGGCTCCGTGTACGATGGGAACAGGCAGTGTGAGCTCATGTTT
  GGCCCTGGATCACAAGTGTGTCCCTACTTGAAGCACTGCCGACGTCTGTGGTGCACGAGTGCAGAAGGCG
  TCCACAAGGGCTGTCGCACACAGCACATGCCGCTGGCAGATGGGACGAGCTGTGGTCCCGGGATGCACTG
  CCACCGTGGGCTGTGTGTGACCAGGGACATGGAGACCCGTCCTGTGGATGGCGAGTGGGGACCATGGGGA
  CCCTACAGCTCGTGTTCAAGGACATGCGGAGGTGGAATCAAGAGCACAGCCCGGCTCTGTGACCGGCCCG
  AGCCACGAAACGGAGGAAGGTACTGTGTGGGCCGCAGGATGAAATTTCGATCATGTAATACTGATTCATG
  TCCAAAAGGCAAGCGAGACTTCCGAGAAAAGCAATGCTCCGACTTCGACGGTAAACATTTCGACATCAAC
  GGCCTCCCCCCCAATGTGAGGTGGCTTCCGAAGTACAGCGGGATTGCCGTAAAAGATCGCTGTAAACTCT
  ATTGCCGGGTCGCTGGGACTACCAGCTTCTACCAGCTGAAGGACAGAGTTGCTGATGGGACACCCTGTGG
  GACGGAAACTAACGACATCTGTGTCCAGGGCCTCTGTCGGCAAGCTGGCTGTGATCACGTGTTAAACTCC
  AAGGCCAAGAGAGACAAGTGTGGAGTGTGTGGTGGTGACAACTCTTCGTGCCAGACCCTGGCAGGTGTCT
  TCAACAGTGCCCATTACGGTTACAATGTGGTTGTAAAGATCCCTGCGGGGGCTACAAACATTGAAATCCT
  TCAGCACAGCTATTCTGGAAGACCAGAAGATGACAACTATCTCGCATTATCTGACACTCAAGGGAACTTT
  CTCCTGAATGGAAACTTTGTTGTAAGTATGGCGAAGAAAGAGATCAACATCCAGGGTGCTGTTTTCGAAT
  ACAGCGGGTCAAACAACTCGATTGAAAGAATTAACAGCACGGACCGGCTGGAGGCGGAGCTCGTTCTGCA
  GGTGTTGTGTGTGGGTAATTTATACAACCCTGACGTGCGCTATTCCTTCAACATCCCCATTGAGGAGAGG
  AGCAATCTGTTCTCCTGGGATCCATACGGGCCGTGGCAAGACTGTACCAAAATGTGCCAAGGTCTTCATA
  GAAGAAAAATTGCCTGCGTCCGTAAGAGCGACCATGCAGTTGTATCTGACCACAATTGTGGCCACTTACC
  GATGCCACTGTTTGTGACTGAAAAGTGCAATATGGACTGTGAACTCAGGTGGCACATCATTGGGAAAAGC
  GACTGCTCATCCCAGTGTGGCCAAGGATACAGAACCCTAGACGTTCACTGCATGAAGTACTCGGTTCATA
  AAGGACAGGCTGTCCCAGTAGGAGATCAGTACTGTGGTGACCAGCTCAAGCCTCCCAGCCGAGAGCCATG
  CCATGGCAGCTGTGTTTTGACAAGGTGGCATTATTCAGAGTGGTCCCAGTGTTCTAGGAGTTGTGGCGGC
  GGTGACAAGACTCGGGAATCCTACTGCGTAAATGGCTTTGGCCACCGCCTTGCTGAAAGCGAGTGTCGGG
  AGCTTCCGCGGGTGGTGCTGGAGAACTGCAATGAATTTCCGTGTCCCAGCTGGGCAACGAGTGAGTGGAG
  TGAGTGTCCTGTCACATGTGGAAAAGGAATGAAGCAGCGACAGGTTTGGTGTCAGCTGAGTGAAGACCCC
  ATGAGGGATGGTTTCTGTAATGCCAGTACCAAGCCCGAGTCGCTGAGACCCTGTGAGCTCCGTGCGTGCG
  CTTCTTGGCATGTAGGACCGTGGGGGTCTTGCACGGCCACCTGTGGACATGGATATCAGATGCGTGCTGT
  TAAGTGTATCAGTGAGATATTTGGCACCATGCTAGATGACAGAGAGTGCCCGCAAGCCAGCCGGCCAAGT
  GACAGACAGGACTGTATATTGGCACCTTGCCTGGCTATTCCAGAAGTGGGGGCCACCTCCTTACCAGCCA
  TTCCCCTGGGAAGGGCAGCACAGTGGCGCCATGGATCGTGGACCCCATGCTCTGTGTCCTGTGGAAGAGG
  CAGTCAGGCCCGTTATGTGAGCTGCCGGGATGCTCATGATGAAGTGGCAGATGAGTCGAATTGTGCCCAC
  TTGCCTCGACCTGCTGCGGTGTCTCTCTGCTTCAGCCCTTGTGGAGAGTGGCAAGCAGGGGACTGGTCAC
  CATGTTCAGCCTCCTGTGGACATGGGAAAACCACCCGACGTGTTTTATGCGTGAACTACCACCAACTGGT
  CGATGAGAGTTACTGTGACCCGGAAGGCCGTCCTGTGACTGAACAAGAATGTAGCCTGGCAGCCTGCCCA
  CCCTTATACAGCCGAGCTCCCAGCTCCTCTGAGCAGCCAAGCCACGTTCCCAGCAGAAATGTTCCATTAA
  CTCACAAACCAGGAGAGAATCAAGATCAGGGGGCCCAGCTGTCAATCAGAGGAAACCAGTGGAGAACAGG
  CCCTTGGGGAGCATGCTCCAGAAGCTGCGCCGGTGGTCTTCAGCACAGGGCTGTGGTCTGCCAGGATGAA
  GACGGGCGAAGCGCTACTTCCTGTGATGGGTCCTCCAAGCCCCCAGAGTCAAGGCACTGTGGCTCCGGAC
  CTTGTCCGCACTGGAATTATGGGGACTGGGGAGAATGCACACAAACCTGTGGTGGAGGGGTAAAATCAAG
  ATTTGTGATCTGTCAGTTTCCCAATGGCCAAATGACACAGGAACACAGCTGTGAACTGCCCAAGCCACCT
  AGCATGATGCAGTGTCACCTGCACGCCTGCCCTGAAGATGTGTCCTGGTATCGGGGACCATGGAAGTCGG
  TACGATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001164785
• Insert Size: 4278 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001164785.1, NP_001158257.1
• RefSeq Size: 6988 bp
• RefSeq ORF: 4278 bp
• Locus ID: 223838
• Cytogenetics: 15 48.2 cM
• Gene Summary: This gene encodes a member of "a disintegrin and metalloproteinase with thrombospondin motifs" (ADAMTS) family of multi-domain matrix-associated metalloendopeptidases that have diverse roles in tissue morphogenesis and pathophysiological remodeling, in inflammation and in vascular biology. The encoded preproprotein undergoes proteolytic processing to generate an active protease. Certain mutations in this gene cause defective development of neural crest-derived melanoblasts resulting in a "belted" phenotype that is characterized by white spots in the lumbar region. [provided by RefSeq, Jul 2016]
  Transcript Variant: This variant (2) differs in the 3' coding region and 3' UTR, compared to variant 1. The resulting isoform (2) has a distinct C-terminus and is shorter than isoform 1. This isoform (2) may undergo proteolytic processing similar to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.