Kdm6a (NM_009483) Mouse Untagged Clone

CAT#: MC224431

Kdm6a (untagged) - Mouse 4lysine (K)-specific demethylase 6A (Kdm6a), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Kdm6a
• Synonyms: Utx
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224431 representing NM_009483
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAATCCTGCGGAGTGTCGCTCGCTACCGCCGCCGCCGCCGCCGCCGCCGCCGCTTTCGGTGATGAGG
  AAAAGAAAATGGCGGCGGGAAAAGCGAGCGGCGAGAGCGAGGAGGCGTCCCCCAGCCTGACAGCGGAGGA
  GAGGGAGGCGCTCGGCGGACTGGACAGCCGCCTTTTCGGGTTCGTGAGGTTTCATGAAGATGGCGCCAGG
  ATGAAGGCCCTGCTGGGCAAGGCTGTTCGCTGCTACGAATCTCTAATCTTAAAAGCTGAAGGGAAAGTGG
  AGTCTGATTTCTTTTGTCAATTAGGTCACTTCAACCTCTTATTGGAAGATTATCCAAAAGCATTATCTGC
  ATACCAGAGGTACTACAGTTTACAGTCTGATTACTGGAAGAATGCTGCCTTTTTATATGGTCTTGGTTTG
  GTCTACTTCCATTACAATGCATTTCAGTGGGCTATTAAAGCATTTCAGGAGGTGCTTTATGTCGATCCCA
  GCTTTTGTCGAGCCAAGGAAATTCATTTACGACTTGGGCTTATGTTCAAAGTGAACACAGACTATGAGTC
  TAGTTTAAAGCATTTTCAGTTAGCTTTGGTTGACTGTAATCCCTGCACTTTGTCCAATGCTGAAATTCAG
  TTTCACATTGCCCACTTATATGAAACCCAGAGGAAGTATCATTCTGCAAAAGAAGCTTATGAGCAACTTT
  TGCAGACAGAAAACCTTTCTGCACAAGTAAAAGCAACTATTTTACAACAATTAGGTTGGATGCATCACAC
  TGTGGATCTCCTGGGAGATAAGGCCACCAAGGAAAGTTATGCTATTCAGTATCTCCAGAAGTCCTTGGAA
  GCAGATCCAAATTCTGGCCAGTCCTGGTATTTCCTTGGAAGGTGCTATTCAAGTATTGGGAAAGTTCAGG
  ATGCCTTTATATCTTACAGGCAATCTATTGATAAATCAGAAGCAAGTGCAGATACATGGTGTTCAATAGG
  TGTGCTCTATCAACAGCAAAATCAGCCTATGGATGCTTTGCAAGCTTATATTTGTGCTGTACAATTGGAC
  CACGGTCATGCTGCAGCCTGGATGGATCTAGGCACTCTCTATGAATCCTGCAACCAACCTCAGGATGCTA
  TTAAATGCTATTTAAATGCAACTAGAAGCAAAAATTGTAGTAATACCTCTGGACTTGCAGCACGAATTAA
  GTATTTACAGGCTCAGTTGTGTAACCTTCCACAAGGTAGTCTACAGAATAAAACTAAATTACTTCCTAGT
  ATTGAGGAGGCATGGAGCCTACCAATCCCCGCAGAGCTTACCTCCAGGCAGGGTGCCATGAACACAGCAC
  AGCAGAATACTTCTGATAATTGGAGTGGTGGCAATGCACCACCTCCAGTAGAACAACAAACTCATTCATG
  GTGTTTGACACCACAGAAATTACAGCACTTGGAACAGCTCCGAGCAAACAGAAATAATTTAAATCCAGCA
  CAGAAACTAATGCTGGAACAGCTGGAAAGTCAGTTTGTCTTAATGCAGCAACACCAAATGAGACAAACAG
  GAGTTGCACAGGTACGGCCTACTGGAATTCTTAATGGGCCAACAGTTGACTCATCACTGCCTACAAACTC
  AGTTTCTGGCCAGCAGCCACAGCTTCCTCTGACCAGAATGCCTAGTGTCTCTCAGCCTGGAGTCCACACT
  GCCTGTCCTAGGCAGACTTTGGCCAATGGACCCTTTTCTGCAGGCCATGTTCCCTGTAGCACATCAAGAA
  CACTGGGAAGTACAGACACTGTTTTGATAGGCAATAATCATGTAACAGGAAGTGGAAGTAATGGAAACGT
  GCCTTACCTGCAGCGAAACGCACCCACTCTACCTCATAACCGCACAAACCTGACCAGCAGCACAGAGGAG
  CCGTGGAAAAACCAACTATCTAACTCCACTCAGGGGCTTCACAAAGGTCCGAGTTCACATTTGGCAGGTC
  CTAATGGTGAACGACCTCTATCTTCCACTGGGCCTTCCCAGCATCTCCAGGCAGCTGGCTCTGGTATTCA
  GAATCAGAATGGACATCCCACCCTGCCTAGCAATTCAGTAACACAGGGGGCTGCTCTCAATCACCTCTCC
  TCTCACACTGCTACCTCAGGTGGACAACAAGGCATTACCTTAACCAAAGAGAGCAAGCCTTCAGGAAACA
  CATTGACGGTGCCTGAAACAAGCAGGCAAACTGGAGAGACACCTAACAGCACTGCCAGTGTTGAGGGACT
  TCCTAATCATGTCCATCAGGTGATGGCAGATGCTGTTTGCAGTCCTAGCCATGGAGATTCTAAGTCACCA
  GGTTTACTAAGTTCAGACAATCCTCAGCTCTCTGCCTTGTTGATGGGAAAAGCTAATAACAATGTGGGTC
  CTGGAACCTGTGACAAAGTCAATAACATCCACCCAACTGTCCATACAAAGACTGATAATTCTGTTGCCTC
  TTCACCATCTTCAGCCATTTCCACAGCAACACCTTCTCCTAAGTCCACTGAACAGACAACCACAAACAGT
  GTTACCAGCCTTAACAGCCCTCACAGTGGGCTGCACACAATTAATGGAGAAGGAATGGAAGAATCTCAGA
  GCCCCATTAAAACAGATCTGCTTCTAGTTAGCCACAGACCTAGTCCTCAGATCATACCATCAATGTCTGT
  GTCCATATATCCCAGCTCAGCAGAAGTTCTGAAAGCTTGCAGGAATCTAGGTAAAAACGGCCTGTCTAAT
  AGTAGCATTCTGTTGGATAAATGTCCGCCTCCAAGACCACCATCCTCACCATACCCTCCCTTGCCAAAGG
  ACAAGTTGAATCCACCTACACCTAGTATTTATTTGGAAAATAAACGTGATGCTTTCTTTCCTCCATTACA
  TCAATTTTGTACAAACCCAAACAACCCTGTTACAGTAATACGTGGCCTTGCTGGAGCTCTTAAATTAGAC
  TTGGGACTTTTCTCTACTAAAACTTTGGTGGAAGCTAACAATGAACATATGGTAGAAGTGAGGACACAGT
  TGTTACAACCAGCAGATGAAAATTGGGACCCTACTGGAACCAAGAAAATCTGGCACTGTGAAAGTAATAG
  ATCTCATACTACAATTGCTAAATATGCTCAGTACCAGGCCTCCTCATTCCAAGAATCATTGAGAGAAGAA
  AATGAGAAAAGAAGTCACCATAAAGACCACTCAGACAGTGAATCTACATCATCAGATAATTCTGGGAAAA
  GAAGAAAAGGACCCTTTAAAACCATTAAGTTTGGGACCAACATTGACCTGTCTGATGACAAAAAGTGGAA
  GTTACAGCTACATGAGCTGACTAAACTTCCTGCCTTCGTGAGAGTTGTATCTGCAGGAAATCTTTTAAGC
  CACGTTGGTCATACTATACTGGGCATGAACACAGTTCAACTATACATGAAAGTTCCAGGAAGCAGAACAC
  CAGGTCATCAAGAAAATAACAACTTCTGTTCAGTTAATATAAATATTGGCCCAGGTGACTGTGAATGGTT
  TGTTGTTCCTGAAGGCTACTGGGGTGTTTTGAATGACTTCTGTGAAAAAAATAATTTGAATTTCTTAATG
  GGTTCTTGGTGGCCCAACCTTGAAGATCTATATGAAGCAAATGTTCCAGTGTATAGGTTTATTCAGCGAC
  CTGGAGATCTGGTCTGGATAAATGCTGGCACTGTTCATTGGGTTCAAGCTATTGGCTGGTGCAACAATAT
  TGCTTGGAATGTTGGTCCACTTACAGCCTGTCAGTATAAGTTAGCAGTGGAACGTTATGAATGGAACAAG
  TTGCAAAATGTAAAGTCAATAGTACCCATGGTTCATCTTTCCTGGAATATGGCACGAAATATCAAGGTTT
  CAGATCCAAAGCTTTTTGAAATGATTAAGTATTGTCTTCTGAGAACGCTGAAGCAATGTCAGACATTGAG
  GGAAGCTCTAATTGCTGCAGGAAAAGAGATCATATGGCACGGGCGGACAAAAGAAGAACCAGCTCATTAT
  TGTAGTATTTGTGAGGTGGAGGTTTTTGATCTGCTTTTTGTCACTAATGAGAGTAATTCTCGAAAAACCT
  ACATAGTACATTGCCAAGATTGTGCACGAAAAACAAGTGGGAATCTGGAAAATTTTGTGGTGCTAGAACA
  GTACAAAATGGAGGATCTGATGCAAGTCTATGACCAATTTACATTAGTAAGTGAAATCAACATGCTCCTC
  CATTACCATCCGCCTCATCTTGATATTGTTCCATGGACATTAAACATGAGACCTTTTCTGCTATTCAGAA
  AGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009483
• Insert Size: 4275 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009483.1, NP_033509.1
• RefSeq Size: 5309 bp
• RefSeq ORF: 4275 bp
• Locus ID: 22289
• UniProt ID: O70546
• Cytogenetics: X 13.45 cM
• Gene Summary: Histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-27'. Plays a central role in regulation of posterior development, by regulating HOX gene expression. Demethylation of 'Lys-27' of histone H3 is concomitant with methylation of 'Lys-4' of histone H3, and regulates the recruitment of the PRC1 complex and monoubiquitination of histone H2A (By similarity). Plays a demethylase-independent role in chromatin remodeling to regulate T-box family member-dependent gene expression (PubMed:21095589).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1).