Blm (NM_007550) Mouse Untagged Clone

CAT#: MC224422

Blm (untagged) - Mouse Bloom syndrome, RecQ helicase-like (Blm), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Blm
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224422 representing NM_007550
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGATCATGGCTGCTGTTCCTCTGAACAATCTACAAGAACAACTACAGCGACACTCAGCCAGAAAAC
  TTAATAATCAACCCAGCCTTTCAAAACCAAAATCTTTAGGTTTTACTTTTAAAAAGAAAACATCAGAGGG
  TGATGTGTCTGTCACTAGTGTGTCCGTAGTAAAAACACCTGCGTTAAGTGATAAAGATGTGAACGTGTCT
  GAGGCCTTTTCATTCACTGAGTCTCCACTCCACAAACCAAAGCAGCAGGCAAAGATTGAAGGCTTCTTTA
  AACATTTCCCTGGAAGGCAGCAAAGCAAGGGGACCTGCTCTGAGCCGTCACTGCCGGCCACGGTACAGAC
  TGCTCAGGACACTTTGTGCACTACCCCCAAAACCCCCACTGCGAAGAAACTGCCCGTGGCTGTTTTCAAG
  AAATTAGAATTTAGTTCTTCTGCAGACTCCCTCAGTGACTGGGCTGATATGGATGACTTTGATATGTCAG
  CATCAGATGCGTTTGCTTCACTGGCTAAAAATCCTGCCACAAGAGTAAGCACCGCTCAGAAAATGAAAAA
  GACTAAGAGAAACTTCTTTAAACCACCACCTCGTAAAGCCAATGCAGTAAAGACTGACTTGACTCCTCCC
  TCCCCCGAATGCCTGCAAGTGGATTTAACGAAGGAATCGGAGGAGGAGGAGGAGGAGGAGGAGGAGGCGG
  AGGGGGCGGACTGCCTGAGCAGGGATGTGATCTGCATTGACAATGATTCTGCTTCTGAAGAGCTCACGGA
  GAAAGACACGCAGGAAAGCCAGTCTTTGAAAGCTCACTTGGGAGCTGAAAGAGGTGACAGTGAAAAGAAG
  AGCCATGAAGACGAAGCTGTGTTCCATTCAGTTCAGAACACTGAATACTTTGAACACAATGACAATGATT
  ATGATATAGATTTTGTTCCACCTTCTCCAGAAGAAATCATCTCCACTGCTTCTTCCTCGTTGAAATGTTC
  CAGTATGTTAAAGGATCTTGATGACTCTGACAAAGAAAAGGGCATTCTTAGCACCTCAGAAGAGCTTCTG
  TCAAAACCAGAGGAAATGACCACACACAAGTCTGATGCAGGAACCAGTAAAGACTGTGATGCCCAGCAGA
  TACGCATACAGCAGCAGCTTATTCATGTGATGGAGCACATCTGTAAGTTAGTTGATACTGTTCCTACTGA
  TGAACTGGAAGCTTTGAATTGTGGGACCGAATTGCTTCAACAACGAAACATAAGGAGGAAGCTCCTAGCT
  GAAGCAGGTTTTAATGGAAATGACGTCAGACTTCTGGGTTCTCTGTGGAGGCACAGGCCTGATTCACTTG
  ATAACACAGTGCAGGGCGACTCCTGCCCTGTGGGGCATCCTAATAAAGAGTTAAATTCTCCATACCTTCT
  CTCACATTCCCCTTCCACTGAGGAATGTTTACCCACCACCACTCCAGGAAAGACAGGATTCTCAGCCACC
  CCGAAGAATCTCTTTGAAAGGCCGTTATTGAATTCCCATTTACAGAAGTCCTTTGTAAGTAGCAACTGGG
  CTGAAACACCAAGGATGGAAAACAGGAACGAAAGCACTGACTTCCCAGGGAGTGTTCTCACCAGCACCAC
  TGTGAAAGCTCAGAGTAAACAAGCTGCTTCAGGATGGAACGTAGAGAGACACGGCCAGGCTTCCTATGAT
  ATCGATAACTTTAATATTGATGACTTTGATGATGATGATGATGATGATGACTGGGAAAACATAATGCACA
  ATTTTCCAGCCAGCAAATCTTCCACAGCCACCTACCCACCCATCAAGGAAGGTGGGCCAGTTAAATCTCT
  CTCAGAAAGGATTTCTTCAGCCAAGGCAAAGTTTCTTCCAGTGGTATCAACCGCTCAAAATACAAACCTC
  TCAGAGTCAATTCAGAATTGCTCTGATAAGCTGGCCCAAAATTTATCATCAAAAAATCCAAAACATGAAC
  ATTTTCAAAGTCTTAATTTTCCTCATACAAAAGAAATGATGAAGATTTTCCATAAGAAATTTGGCTTGCA
  TAATTTTAGAACTAATCAGCTAGAGGCGATCAATGCTGCGCTGCTTGGTGAAGACTGCTTTATCCTAATG
  CCCACTGGAGGAGGTAAAAGTTTGTGCTACCAGCTCCCTGCCTGTGTTTCTCCTGGGGTCACAATTGTCA
  TTTCTCCCTTGAGATCACTAATAGTAGATCAAGTCCAAAAGCTGACTTCCTTTGATATTCCAGCTACATA
  TCTGACAGGGGATAAGACTGACTCAGAAGCTGCAAATATTTACCTCCAATTATCCAAAAAAGACCCAATC
  ATCAAGCTTTTGTATGTTACTCCAGAGAAGGTCTGTGCAAGTAACAGGCTGATTTCTACTCTGGAGAATC
  TGTATGAGCGGAAGCTCTTGGCACGTTTTGTCATTGATGAAGCGCATTGTGTGAGTCAGTGGGGTCATGA
  TTTTCGTCAAGATTACAAAAGGATGAATATGCTTCGCCAGAAGTTTCCTTCTGTTCCAGTGATGGCCCTC
  ACGGCCACAGCGAACCCCAGGGTCCAGAAGGACATCCTCACTCAGCTGAAGATCCTCAGACCTCAGGTGT
  TTAGCATGAGCTTTAACAGACACAATCTGAAGTACTATGTATTACCCAAGAAGCCCAAAAAAGTAGCATT
  TGATTGCCTAGAGTGGATCAGAAAGCATCACCCTTATGACTCGGGGATAATTTACTGCCTCTCCAGGAGG
  GAATGTGACACAATGGCTGACACTTTACAGAGAGAAGGCCTGGCTGCCCTGGCTTACCATGCGGGCCTCA
  GTGACTCTGCCAGAGATGAGGTGCAGCACAAGTGGATCAACCAGGACAACTGCCAGGTTATCTGTGCGAC
  AATTGCGTTTGGAATGGGAATTGACAAACCTGACGTGCGATTTGTGATTCATGCATCTCTTCCTAAATCT
  ATGGAGGGTTATTATCAAGAATCCGGCCGAGCTGGAAGAGATGGGGAAATATCTCACTGCGTGCTTTTCT
  ATACATATCATGATGTGACCAGACTGAAGAGACTTATAATGATGGAAAAAGATGGAAACTATCATACGAA
  GGAAACTCACGTCAATAATCTATATAGCATGGTACATTACTGTGAAAACATAACGGAATGCAGAAGAATA
  CAGCTTTTAGCTTACTTTGGTGAAAAAGGATTCAACCCTGATTTTTGTAAGAAATACCCAGATGTTTCTT
  GTGACAATTGCTGTAAAACAAAGGATTATAAAACAAAAGATGTGACTGATGACGTGAAAAATATTATAAG
  ATTTGTTCAAGAACACAGTTCATCACCAGGAACAAGAAATATAGGACCTGCTGGAAGATTTACTCTGAAC
  ATGCTGGTCGACATTTTCTTGGGGAGCAAGAGTGCAAAAGTTAAGTCTGGAATATTTGGAAAGGGGACTA
  CATATTCACGACATAATGCCGAAAGACTTTTTAAAAAGCTGATTCTAGACAAAATCCTGGATGAAGACTT
  ATATATCAATGCCAATGACCAACCAATTGCCTATGTGATGCTAGGAACAAAAGCCCACAGTGTGCTGAGT
  GGCCACTTGAAGGTGGACTTCATGGAAACGGAAAATTCCAGCAGTATTAAAAAACAAAAAGCTTTAGTGG
  CCAAAGTATCCCAGAGAGAAGAGGTAGTTAAGAAATGTCTTGGAGAACTTACAGAGGTCTGCAAATTGCT
  GGGGAAAGTCTTTGGTGTCCATTACTTCAATATTTTTAATACAGCCACACTCAAAAAGCTTGCAGAATCT
  TTATCTTCTGATCCTGAGGTTTTGCTTCAGATTGATGGTGTTACCGAAGACAAGCTGGAAAAATATGGTG
  CAGAAGTGATTCCAGTATTACAGAAGTACTCAGAATGGACAGTGCCAGCTGAGGATGGTTCCCCAGGCGC
  CAGAGGCGCCCCAGAGGACACTGAGGAGGAGGAGGAGGAAGCGCCTGTATCTTCTCACTACTTTGCAAAT
  CAAACTAGAAATGAAAGAAAGAGAAAGAAAATGTCAGCCACCCATAAGCCCAAGAGGAGAAGAACTAGTT
  ACGGTGGCTTCAGAGCAAAGGGGGGCTCTACTACATGCAGAAAAACGACTTCTAAAAGTAAATTCTATGG
  TGTAACTGGATCCCGCTCGGCCTCATGTGCTTCTCAGGCAACATCATCAGCCAGTAGAAAACTGGGGATT
  ATGGCTCCTCCAAAGCCTGTAAATAGAACGTTCCTCAGGCCTTCATATGCCTTCTCCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_007550
• Insert Size: 4260 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007550.4, NP_031576.4
• RefSeq Size: 4869 bp
• RefSeq ORF: 4260 bp
• Locus ID: 12144
• UniProt ID: O88700
• Cytogenetics: 7 45.65 cM
• Gene Summary: ATP-dependent DNA helicase that unwinds single- and double-stranded DNA in a 3'-5' direction (PubMed:9840919). Participates in DNA replication and repair (By similarity). Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA (PubMed:9840919). Negatively regulates sister chromatid exchange (SCE) (PubMed:9840919, PubMed:27010503). Stimulates DNA 4-way junction branch migration and DNA Holliday junction dissolution. Binds single-stranded DNA (ssDNA), forked duplex DNA and DNA Holliday junction (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).