Dcaf1 (NM_001015507) Mouse Untagged Clone

CAT#: MC224421

Vprbp (untagged) - Mouse Vpr (HIV-1) binding protein (Vprbp), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dcaf1
• Synonyms: AI447437; B930007L02Rik; mKIAA0800; Vprbp
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224421 representing NM_001015507
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTACAGTAGTGGTACATGTGGACTCCAAAGCTGAGCTCACTACCCTGCTGGAGCAGTGGGAAAAGG
  ACCATGGCAGTGGCCAGGATATGGTACCAATCCTTACCAGAATGTCTGAATTAATTGAAAAAGAAACTGA
  AGAATATCGTAAAGGAGATCCAGATCCATTTGATGATCGACATCCTGGTCGAGCTGATCCAGAGTGTATG
  CTGGGCCATTTGCTGAGAATACTCTTCAAGAATGATGATTTCATGAATGCGCTGGTAAATGCATATGTGA
  TGACAAGTAGAGAGCCCCCTTTAAATACTGCAGCTTGCAGACTGCTGCTGGACATCATGCCGGGCCTAGA
  AACCGCAGTTGTCTTTCAAGAAAAGGAAGGAATTGTTGAAAATCTTTTCAAATGGGCCCGAGAGGCTGAT
  CAGCCACTTAGGACATATTCTACTGGACTGCTGGGAGGTGCTATGGAAAATCAAGATATTGCTGCCAACT
  ACAGAGATGAAAATTCACAGTTGGTGGCAATAGTGCTTAGAAGACTGAGGGAGCTCCAGCTTCAAGAAGT
  GGCTTTGCGGCAGGACAGTAAGCGTCCTAGTCCACGGAAGCTCTCTTCGGAACCCCTTTTGCCTCTGGAT
  GAGGAAGCTGTGGATATGGACTATGGTGACATGGCAGTGGATGTTGTGGATGGAGAACAAGAATCTTCTA
  GAGATATGGAGATCTCCTTTCGTCTTGATTCAAGTCACAAGACCAGTAGTAGAGTGAACTCAGCAACTAA
  ACCTGAGGAAGGAGGATTAAAGAAAAACAAGTCAGCAAAGCATGGTGACAGAGAGAACTTTAGGAAGGCC
  AAGCAGAAGTTGGGCTTCTCATCTTCTGACCCAGATCGTGTGTTTGTTGAGCTGTCTAATAGCAGCTGGT
  CAGAAATGTCCCCCTGGGTGATCGGCACCAATTACACTCTCTATCCTATGACTCCTGCTATTGAGCAGAG
  ACTTATTCTTCAGTATTTGACCCCTTTAGGAGAGTATCAGGAGTTACTTCCCATATTCATGCAACTTGGA
  TCAAGAGAATTGATGATGTTTTATATTGATCTGAAACAGACGAATGATGTCCTGCTTACTTTTGAGGCAC
  TCAAGCACCTAGCATCTCTCCTGCTTCATAACAAATTTGCCACAGAATTTGTTGCACATGGTGGAGTACA
  GAAATTACTGGAAATTCCTCGTCCTTCTATGGCTGCAACTGGTGTGTCCATGTGCTTGTATTACCTGTCC
  TACAATCAGGATGCTATGGAAAGAGTTTGCATGCATCCTCACAATGTTCTATCTGATGTGGTGAACTATA
  CCCTGTGGTTAATGGAATGTTCTCATGCTTCAGGATGTTGCCATGCTACTATGTTTTTCTCAATTTGTTT
  CTCATTCCGGGCTGTTTTGGAGCTCTTTGACCGTTATGATGGTCTTCGTCGTCTGGTGAACTTGATCAGT
  ACTTTGGAGATTTTAAATTTGGAAGACCAGGGTGCACTTTTGAGTGATGATGAAATATTTGCTAGTCGCC
  AAACAGGAAAACATACCTGCATGGCTCTGCGCAAGTATTTTGAGGCTCATCTGGCCATTAAATTGGAACA
  AGTCAAGCAGTCACTTCAGAGGACTGAGGGTGGTATTCTTGTTCACCCCCAGCCTCCATACAAGGCATGC
  TCATATACTCATGAGCAGATTGTAGAAATGATGGAATTTTTGATAGAGTATGGCCCAGCTCAGCTATATT
  GGGAACCTGCTGAAGTCTTCCTCAAGCTTTCCTGTGTGCAGCTTTTACTACAGCTTATTTCCATCGCCTG
  CAATTGGAAGACCTATTATGCAAGGAATGATACTGTGCGTTTTGCTTTGGATGTCCTTGCTATTCTCACT
  GTGGTGCCAAAAATCCAGCTCCAGTTGGCGGAGTCAGTGGATGTACTGGACGAGGCCGGCTCTGCTGTCT
  CCACTGTAGGTATCAGCATTATTTTGGGCGTGGCTGAGGGTGAGTTCTTTATCCATGATGCTGAAATTCA
  GAAGTCAGCACTTCAAATTATCATCAATTGTGTATGTGGTCCAGATAACCGAATATCCAGTATTGGCAAA
  TTTATTTCTGGAACTCCTCGGAGAAAGCTGTCTCAGACTCCCAAGAGTAGTGAGCATACTCTGGCCAAGA
  TGTGGAATGTGGTCCAGTCCAACAATGGCATCAAGGTGCTTCTGTCCCTGTTGTCCATCAAGATGCCCAT
  TACAGACGCAGACCAGATCCGGGCCCTGGCCTGCAAAGCCCTTGTAGGCCTGTCTCGAAGTAGCACTGTT
  CGACAGATCATCAGTAAACTTCCCCTTTTTAGTAGCTGCCAGATTCAACAGCTGATGAAAGAGCCTGTGC
  TGCAGGATAAGCGTAGTGACCATGTCAAGTTCTGCAAATACGCTGCTGAGCTCATTGAGCGTGTATCAGG
  AAAGCCTCTTCTCATTGGCACTGATGTGTCTCTCGCACGACTGCAGAAAGCAGATGTTGTTGCTCAGTCG
  AGGATCTCCTTCCCTGAGAAAGAGCTGCTCCTGTTGATACGGAACCATCTCATTTCTAAAGGGCTTGGAG
  AGACAGCAACTGTGCTGACGAGAGAGGCTGACCTGCCCATGACTGCTGCCTCACATTCTTCTGCCTTCAC
  CCCAGTGACTGCTGCTGCTTCTCCTGTCTCTCTTCCCCGGACCCCTCGGATTGCCAACGGCATTGCAAGT
  CGACTGGGCAGCCATGCTACTGTGGGTGCTTCTGCCCCTTCTGCTCCCCCTGCCCATCCTCCACCACGGC
  CCCCCCAGGGTTCATTGCCTTTGCCTGGCCCATCATATGCAGGCAATTCCCCTTTGATTGGTAGGATCAG
  TTTTATCAGAGAGAGGCCATCACCGTGCAATGGCAGGAAAATTCGAGTGTTGCGGCAGAAGTCGGACCAT
  GGTGCATACAGCCAGAGCCCTGCCATAAAAAAACAATTGGATAGACATCTTCCTTCCCCACCTACGCTGG
  ACAGTATCATCACAGAGTATCTTAGAGAGCAACATGCTCGATGCAAGAACCCCGTTGCCACCTGTCCTCC
  TTTTTCTCTCTTTACTCCTCATCAGTGTCCTGAACCAAAACAGAGGCGGCAAGCGCCAATAAACTTTACC
  TCAAGGCTAAACCGCAGGGCATCATTTCCAAAATATGGAGGGGTAGATGGCGGATGCTTTGATAGGCACC
  TTATCTTTAGCAGATTTCGTCCGATTTCAGTATTCCGGGAAGCTAATGAAGATGAAAGTGGCTTCACCTG
  TTGTGCATTCTCAGCCCGGGAGCGGTTCCTGATGCTTGGCACTTGTACAGGGCAGCTGAAGCTCTACAAT
  GTGTTTAGTGGACAGGAAGAGGCCAGCTATAACTGTCACAACTCAGCCATCACACACCTTGAACCGTCAA
  GGGATGGGTCCTTACTTCTGACATCTGCCACTTGGAGCCAGCCTTTGTCTGCACTTTGGGGAATGAAATC
  AGTATTTGATATGAAGCATTCCTTCACAGAAGATCACTATGTTGAGTTCAGTAAGCACTCTCAGGATCGG
  GTCATAGGCACAAAAGGAGACATTGCCCATATTTATGATATTCAGACTGGCAACAAGCTGTTGACTCTGT
  TTAACCCAGATCTTGCCAACAACTACAAGAGGAACTGTGCCACCTTTAATCCTACAGATGATCTTGTCTT
  AAATGATGGCGTCCTCTGGGATGTCCGCTCTGCACAGGCCATCCACAAGTTTGACAAGTTCAACATGAAT
  ATCAGTGGTGTTTTCCATCCAAATGGGCTGGAAGTCATCATCAATACAGAGATTTGGGACCTTCGGACTT
  TCCATCTTTTACACACTGTTCCTGCTCTGGATCAATGCCGTGTTGTTTTTAACCACACTGGGACAGTGAT
  GTATGGAGCAATGTTGCAGGCAGATGATGAAGATGACTTACTGGAAGAGAGGATGAAAAGCCCTTTTGGG
  TCATCCTTTCGAACATTCAACGCAACTGATTATAAACCTATAGCAACTATTGATGTGAAACGAAACATCT
  TTGACCTGTGCACAGACACCAAAGACTGTTATCTTGCTGTCATTGAGAATCAAGGCAGCATGGATGCACT
  GAATATGGACACAGTGTGCAGGCTCTATGAAGTGGGCAGGCAGCGTCTGGCAGAGGATGAGGATGAAGAG
  GAGGACCAGCAGACAGCTCGGACAACTCTGATTTGGAAGATGACATCATCTTATCTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001015507
• Insert Size: 4260 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001015507.2, NP_001015507.1
• RefSeq Size: 5392 bp
• RefSeq ORF: 4260 bp
• Locus ID: 321006
• UniProt ID: Q80TR8
• Cytogenetics: 9 F1
• Gene Summary: Acts both as a substrate recognition component of E3 ubiquitin-protein ligase complexes and as an atypical serine/threonine-protein kinase, playing key roles in various processes such as cell cycle, telomerase regulation and histone modification. Probable substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex, named CUL4A-RBX1-DDB1-DCAF1/VPRBP complex, which mediates ubiquitination and proteasome-dependent degradation of proteins such as NF2. Involved in the turnover of methylated proteins: recognizes and binds methylated proteins via its chromo domain, leading to ubiquitination of target proteins by the RBX1-DDB1-DCAF1/VPRBP complex. The CUL4A-RBX1-DDB1-DCAF1/VPRBP complex is also involved in B-cell development: DCAF1 is recruited by RAG1 to ubiquitinate proteins, leading to limit error-prone repair during V(D)J recombination. Also part of the EDVP complex, an E3 ligase complex that mediates ubiquitination of proteins such as TERT, leading to TERT degradation and telomerase inhibition. Also acts as an atypical serine/threonine-protein kinase that specifically mediates phosphorylation of 'Thr-120' of histone H2A (H2AT120ph) in a nucleosomal context, thereby repressing transcription. H2AT120ph is present in the regulatory region of many tumor suppresor genes, down-regulates their transcription and is present at high level in a number of tumors. Involved in JNK-mediated apoptosis during cell competition process via its interaction with LLGL1 and LLGL2.[UniProtKB/Swiss-Prot Function]