Rpap1 (NM_177294) Mouse Untagged Clone

CAT#: MC224415

Rpap1 (untagged) - Mouse RNA polymerase II associated protein 1 (Rpap1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rpap1
• Synonyms: 1190005L06Rik; A730023M06Rik; AU041788; AW107702; mKIAA1403
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224415 representing NM_177294
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGCTGTCCAGACCGAAGCCTGGAGAGTCAGAGGTGGACCTGCTGCGCTTCCAGAGCCAGTTCCTTG
  AGGCTGGTGCAGCCCCGGCGGTGCAGCTGGTGAAGGGGAGTAGGAGGCATGGTGATGCTCCTCCAGACCG
  GCTCCCACCGCAGGACCATCGGGATGTGGTGATGCTGGACAATCTCCCAGATTTGCCCCCAGCTCTGCTT
  CCCGCTCCTGCAAAAAGAGCAAGACCGAGCCCTGGTCACCCGCTGCCTCACGATGAAGACCCTGAAGAGA
  GGCTGAACAGGCATGATCAGCACATCACTGCTGTCTTGTCTAAGATTGTTGAACGAGATACAAGTTCAGT
  CACTGTGACTCTGCCTGTGCCCAGTGGTGTTGCTTTCCCGCCTGTGTTCCATCGCTCTCAGGAGAGACAG
  GTGAAACCAGCAGCATCTGGTAAAAGGAGCATCTTTGCCCAAGAGATTGCAGCAAGAAGGGTGTCAGGAA
  ACAGGGTGACATCAGCTGAGCAAGTTGTCCCCAGCCTAGACACACCAGAGGGTGCTGTGCCCTGTGAGAC
  ACCCTCCTTCAGGGACAGAAGCAACCAGCTTCCTGGGAGGAGTCATGGCTTCCACAGACCAAATCTAGTC
  ACAGGAAAGGGGCTCAGGAGCAAGGTGGCTGAGCAGGAGGTCCAGACAATCCATGAAGAGAATGTAGCAA
  GACTACAAGCCATGGATCCTGAGGAGATCCTGAAGGAGCAGCAGCAGTTACTGGCTCAACTCGACCCCAG
  CTTGGTTGCCTTCTTGAGATCTCATAGCCAGGTCCAGGAACAAACAGGAACAAAGGCCACCAAGAAGCAG
  AGTCCAAAGAGACCCTCAGTTCTTGTCACTAAAGAAGAGCCTGTCACGTCAACTCGTACAAGGGAGCCTA
  GGACTGGGGACAAGCTGGAAGAAAAGCCGGAGGCCACGGTGGAGGACAAGATGGAAGACAAGCTGCAGCC
  AAGAACCCCAGCACTGAAACTGCCCATGACCCCCAGCAAAGACTGGCTCCACATGGACACCGTAGAGCTG
  GACAAGCTCCACTGGACCCAGGACCTGCCCCCACTCCGGCGGCAGCAGACACAGGAGAGAATGCAGGCTC
  GATTCAGCCTTCAGGGCGAGCTCCTGGCGCCTGATGTGGATCTTCCCACACATCTGGGCCTGCACCACCA
  CGGAGAAGAGGCCGAGCGAGCAGGGTATTCTCTACAGGAGCTGTTCCACCTGACCCGTAGCCAGGTGTCC
  CAGCAGAGAGCACTAGCATTGCAAGTGTTGTCCCAGATCGTCGGCAGGGCCCAGGCTGGTGAGTTTGGGG
  ACCGCCTAGTGGGCAGTGTCTTGCGCCTCCTCTTGGATGCTGGTTTCCTCTTCCTGCTGCGCTTCTCTCT
  GGATGACAGGGTGGACAGTGTCATCGCAGCAGCTGTCCGGGCTCTTCGTACTCTGCTAGTGGCTCCTGGA
  GATGAGGAGCTCCTGGACCGCACCTTCTCTTGGTATCATGGAGCTTCGGTGTTCCCTCTGATGCCCAGCC
  AGGACGATAAAGAGGATGAAGATGAAGATGAGGAACTCGAAACAGAAAAGGTGAAGAGAAAGACACCTGA
  GGAAGGAAGCCGCCCTCCCCCGGACCTGGCCAGACATGATGTCATCAAGGGGCTCCTGGCTACCAACCTG
  CTTCCTCGGCTCCGCTATGTGCTGGAGGTGACCTGCCCAGGACCCTCTGTGATCCTTGACATCCTGGCTG
  TGCTTATCCGCCTGGCCCGGCATTCCCTGGAGTCGGCCATGAGGGTCCTGGAGTGTCCTCGGCTGATGGA
  GACCATAGTTCAAGAGTTCCTGCCTACCAGTTGGTCCCCTATAGGGGTGGGGCCTACTCCCAGTCTATAT
  AAAGTGCCTTGTGCTTCTGCCATGAAACTGCTTAGAGTCCTGGCTTCAGCTGGGAGGAATATTGCTGCTC
  GACTGCTAAGCGGCTTTGATGTCAGGAGCCGCCTGTGCCGATTCATAGCTGAAGCGCCACATGACCTGGC
  CTTGCCCCCCGAGGAAGCAGAGATTCTGACCACTGAGGCCTTCCGTCTGTGGGCCGTGGCTGCCTCCTAT
  GGCCAGGGTGGTGACCTTTACAGGGAGCTGTACCCAGTGCTGCTGCGGGCCTTGCAGACACTGCCTACAG
  AGCTCAGCGCTCATCCCCTACAGCCCCTGGCCATGCAGCGGGTGGCCGCTCTGTTCACACTGCTTACCCA
  GCTCACCCTGGCAGCTAGCAGCATACCTCCTGAGCCCGCCAGTGGCCCTGCTGAGTCCTGTGTGCCGGCC
  ATCCCTTCCTCAGTCACCTGGACACAGGTGTCTGGACTCAAGCCACTGGTGGAGCCATGTCTAAAGCAGA
  CCCTAAAGTTCCTGCCCAGGCCGGATGTGTGGAATGCCCTGGGCCCAGTGCCCAGTGCCTGCCTTCTGTT
  CTTGGGTGCTTACTATCAGGCCTGGAGCCGGCAGTCACACTTGTGCCCAGAAGATTGGCTTCAGGACATG
  GAGCGCTTGTTGGATGAGTCCTTGCTGCCACTGCTGAGCCAGCCTCCTCTGGGCAGTCTGTGGGATTCCT
  TAAGGGACTGCTCCCCACTCTGCAATCCGCTGTCCTGTGCTTCCAACCCGGAAGCCCTCCCCAGCCTTGT
  GTCACTGGGCTGTGCAGGAGGCTGTCCCCCTCTCAGTGTGGCTGGCTCAGCCTCACCCTTCCCGTTCCTC
  ACTGCCCTCCTCTCCCTCATCAACACTCTGGTCCAGAGCCACAAGGGGCTCTGTGGACAGCTGTCTGCTG
  TGTTGACTGCCCCAGGACTCCAGAACTACTTTCTCCAGTGTGTGGCTCCTGCGCCTGCCCCACAGCTCAC
  ACCCTTCTCTGCCTGGGCCCTGCACCATGAGTACCACCTACAGTATCTGGTGCTTTCCTTCGCCCACAAA
  GCGGCAACACTGCAGCCAGAACCAGCTGCCAGCACTGCCCTCCACCATGCTGTAGCCTTGGTCCTGCTGA
  GCCGGCTGTTGCCTGGAAGCGAATACCTTGCCCAGGAGCTGCTGCTGAGCTGTGTGTTCCGGCTGGAGTT
  CCTGCCGGAAAGTGCTTCAGGGGGCCCAGAGGCAGCTGACTTCTCCGATGGACTGTCCTTAGGGAGCAGT
  GGGGACCCTCAGTGTAGACGAGGGGCCCTCCTCGTTCAAGCTTGCCGGGATCTCCCCAGCATCCGCAGCT
  GCTACCTGGCCCATTGTTCACCAGCCCGAGCCAGTCTGCTGAGCTCCCAAGCCTTGTACTGTGGAGAGCT
  ACTGCGAGTCTCAAGTCTGCTACTGCCTGTACCTAAGGAGCCACTGCTGGCCACTGACTGGCCCTTCCAG
  CCGTTGATACATCTCTACCACCGAGCTTCAGATACTCCCTCTGGGCCACCTGCTGCTGACACTGTAGGCG
  TTGCCATGCGGGTCCTACAATGGGTGCTAGTTCTGGAGAGCTGGCGCCCCGAGGTCCTCTGGGCTGTGCC
  CCCTGCTGCCCGCCTAGCACGTCTCATGTGTGTGTACCTGGTGGATAGCGAGCTGTTTCGAGAGACTCCC
  ATACAGCGACTGGTGGCAGCTCTTTTAGCTCGGCTCTGTCGGCCCCAAGTCTTGCCAAACCTCAAGCTGG
  ACTGCCCCCTCCCTGGCCTGACCTCTTTTCCTGACCTTTATGCCAGCTTCTTAGACCATTTTGAGGCTGT
  CTCTTTTGGGGACCACTTGTTTGGGGCCCTGGTCCTCCTTCCTCTGCAGCGTAGGTTCAGTGTTACCTTG
  CGCCTCGCCCTCTTTGGGGAGCACGTGGGAGTGTTGCGAGCACTTGGCCTGCCTCTGACTCAGCTGCCTG
  TGCCTCTGGAGTGCTACACAGAGCCTGCTGAAGACAGCCTGCCTCTCCTTCAGCTCTACTTCCGGGCCTT
  GGTTACTGGTACACTCCGTGCACGTTGGTGCCCCATCCTCTACACTGTAGCCGTGGCTCATGTCAACAGT
  TTCATCTTCTGCCAAGACCCAAAGAGCTCAGATGAGGTAAAGACTGCCCGAAGGAGTATGCTGCAGAGAA
  CGTGGCTGCTGACAGATGAGGGGCTGCGGCAGCATCTCCTCCACTATAAGCTTCCCAACTCCAGCCTCCC
  AGAGGGCTTTGAGCTGTATTCCCAGTTACCTCGCCTGAGGCAGCAGTGCCTTCAGACACTGCCCACAGAG
  GGGCTCCAGAATGGAGGAGTCAAGACATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_177294
• Insert Size: 4230 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177294.5, NP_796268.3
• RefSeq Size: 4822 bp
• RefSeq ORF: 4230 bp
• Locus ID: 68925
• UniProt ID: Q80TE0
• Cytogenetics: 2 E5
• Gene Summary: Forms an interface between the RNA polymerase II enzyme and chaperone/scaffolding protein, suggesting that it is required to connect RNA polymerase II to regulators of protein complex formation. Required for interaction of the RNA polymerase II complex with acetylated histone H3 (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein.