Eea1 (NM_001001932) Mouse Untagged Clone

CAT#: MC224411

Eea1 (untagged) - Mouse early endosome antigen 1 (Eea1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Eea1
• Synonyms: A430109M19Rik; B230358H09Rik; ZFYVE2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224411 representing NM_001001932
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTCGAAGGATCTTGCAGAGGACTCCTGGGCGAGTTGGCTCTCAAGGTTCTGATTTAGATTCATCAG
  CAACTCCTATAAATACGGTGGATGTCAATAATGAAAGTTCTTCAGAGGGTTTCATATGTCCCCAGTGTAT
  GAAATCCCTTGGCTCTGCTGATGAGCTTTTCAAGCACTATCAGGCCGTTCATGATGCTGGTAATGACTCG
  GGTCATGGTGGAGAGGCCGGTCTTGCTTTGACACGAGATGACATAACACTACTCAGACAAGAGGTCCAAG
  ACCTACAGGCCTCACTTAAGGAAGAAAAATGGTACTCAGAAGAATTAAAGAAAGAATTAGAAAAATATCA
  AGGGCTACAGCAGCAAGAGGCTAAATCTGATGGATTGGTGACTGATTCGTCAGCAGAGCTACAGGCTTTA
  GAGCAACAATTAGAAGAAGCACAAACAGAGAATTTTAATATTAAGCAAATGAAAGATCTGTTTGAACAGA
  AAGCAGCCCAACTTGCTACTGAAATTGCAGATATAAAGTCAAAATATGATGAAGAAAAGAGTCTCCGAGC
  AGCTGCTGAACAAAAAGTGACACATTTGACAGAAGACTTAAACAAACAGACAACTGTAATTCAAGATCTG
  AAAACTGAACTGCTTCAGAGACCTGGTATAGAAGATGTTGCTGTACTAAAGAAAGAACTGGTCCAAGTTC
  AAACACTAATGGATAACATGACTTTGGAACGTGAGAGAGAATCTGAAAAACTTAAAGATGAATGCAAAAA
  GTTACAATCAGAGCATGCTCATTTGGAGGCTACAATAAACCAGCTAAGGAGTGAACTTGCCAAAGGCCCC
  CAAGAAGTTGCAGTATATGTGCAGGAAATACAAAAACTTAAAGGTTCAATTAATGAATTAACACAAAAAA
  ACCAGAACTTGACTGAAAAATTGCAGAAGAAAGACCTAGACTACACCCACTTAGAGGAGAAGCACAATGA
  AGAGTCAGCAAGTCGGAAGACTCTCCAGGCATCCCTCCACCAGCGGGACCTGGATTGCCAGCAGCTTCAG
  GCAAGGCTAACTGCATCTGAAAGCTCACTCCAGAGGGCCCAGGGAGAACTCAGTGAAAAGGCAGAGGCTG
  CTCAGAAGCTCAGAGAAGAACTGCGGGAAGTAGAGAGCACGCGCCAGCATCTCAAGGTGGAGGTGAAGCA
  GCTGCAGCAGCAGCGGGAAGAGAAGGAACAGCACGGGCTGCAGCTGCAGGGGGAAGTCAGCCAGTTACAC
  TGCAAACTTCTGGAGACCGAGCGCCAACTGGGGGAAGCTCACGGCAGGCTGAAGGAACAGCGGCAGCTTT
  CCAGCGAGAAGCTGATGGAAAAGGAGCAGCAGGTGGCTGACCTACAGCTCAAGCTCTCTCGTCTGGAGGA
  GCAGTTGAAGGAAAAGGTAACAAATTCCACTGAATTACAACATCAGTTAGAAAAATCAAAGCAACAGCAT
  CAAGAACAGCAAGCTCTACAGCAAAGTGCTACAGCCAAACTCCGAGAAGCTCAGAATGATTTGGAGCAAG
  TGTTACGTCAGATTGGAGATAAGGACCAAAAGATCCAGAACCTTGAAGCCTTGCTGCAGAAGGGTAAAGA
  AAGCGTTTCCTTACTAGAAAAGGAAAGAGAAGATCTTTATGCAAAAATCCAGGCCGGTGAAGGAGAGACT
  GCTGTTCTTAACCAGTTACAAGAGAAAAATCATGCACTGCAGCAGCAGCTAACTCAACTGACGGAGAAGC
  TGAAGAATCAGTCGGAAAGCCATAAACAAGCCGAGGAGAACTTGCATGACCAGGTACAGGAACAGAAGGC
  ACATCTTCGAGCTGCACAAGACCGTGTTCTTTCTCTGGAAACCAGTGTCAGTGAACTAAGCAGTCAGTTA
  AATGAAAGCAAGGAGAAGGTCTCGCAGCTTGACATACAGATTAAAGCCAAAACTGAATTATTACTATCAG
  CAGAAGCAGCAAAAGCTGCTCAGAGAGCTGATCTTCAGAATCACTTGGACACAGCTCAACATGCGTTACA
  GGATAAACAACAGGAGTTAAATAAGGTCAGTGTGCAGTTGGATCAGCTCACTGCAAAGTTTCAGGAGAAG
  CAGGAGCATTGCATTCAGCTGGAAAGCCACCTTAAGGATCACAAGGAGAAGCACCTCTCTTTAGAACAAA
  AAGTTGAAGACTTAGAAGGCCACATTAAGAAACTAGAAGCTGATGCTCTTGAAGTTAAAGCCAGCAAGGA
  ACAGGCTTTGCAGAGTCTACAGCAGCAGAGGCAGCTGAGCACAGACCTGGAGTTGAGAAATGCAGAGCTG
  AGTAGAGAGCTCCAGGAGCAGGAGGAAGTAGTATCTTGTACAAAACTGGATCTACAGAATAAATCTGAAA
  TCCTTGAAAATATCAAACAAACCCTCACCAAGAAAGAAGAAGAAAATGTCGTCTTGAAACAAGAATTTGA
  AAAGTTAAGTCAAGATTCAAAGACACAGCACAAGGAGCTGGGGGACAGGATGCAAGCTGCGGTCACAGAG
  CTGACAGCAGTGAAGGCACAGAAGGACGCACTCCTGGCCGAGCTTTCTACAACAAAAGAAAAACTGTCAA
  AAGTTTCGGATTCTTTAAAAAACTCCAAAAGTGAATTTGAGAAAGAAAATCAGAAAGGAAAAGCTGCTGT
  ATTAGATTTGGAAAAAGCTTGTAAAGAATTAAAGCATCAGCTTCAAGTACAGGCAGAGAGTGCCCTGAAG
  GAGCAGGAGGACCTGAAAAAGTCACTCGAGAAAGAGAAAGAGACTTCTCAGCAGTTGAAGATAGAACTCA
  ATTCAGTCAAGGGAGAAGTCTCACAGGCTCAAAATACATTAAAGCAAAAGGAGAAAGACGAACAACAGCT
  TCAGGGGACCATAAATCAGCTAAAGCAATCAGCTGAACAAAAGAAAAAACAAATTGAAGCGCTTCAAGGA
  GAGGTTAAAAATGCTGTTTCACAAAAGACAGTGCTGGAGAATAAGCTGCAGCAGCAGTCATCACAGGCAG
  CCCAGGAGCTTGCAGCAGAGAAAGGAAAACTGTCAGCGCTGCAGAGCAACTACGAGAAGTGCCAGGCGGA
  CCTGAAACAGCTGCAGTCCGACCTCTATGGCAAAGAGTCTGAACTTCTAGCCACGAGGCAAGACCTTAAG
  TCTGTAGAAGAGAAGCTCACTCTGGCACAAGAAGACTTGATTTCAAACAGAAATCAAATTGGAAACCAAA
  ATAAATCAATTCAAGAATTGCAAGCCGCCAAAGCTAGTTTGGAGCAGGACTCAGCAAAGAAAGAGGCGCT
  GCTAAAGGAGCAGAGTAAGGCACTTGAGGATGCTCAGAGAGAAAAGTCAGTGAAGGAAAAAGAACTAGTA
  GCTGAAAAGTCTAAATTGGCAGAGATGGAGGAAATTAAATGTAGACAAGAAAAAGAAATCACAAAACTCA
  ATGAGGAGCTCAAGTCGCACAAACAAGAAAGCATAAAGGAAATAACAAATCTCAAGGATGCCAAGCAACT
  TTTGATTCAACAGAAATTAGAACTTCAAGGCAGAGTGGACTCACTGAAGGCAGCTCTTGAGCAGGAGAAG
  GAGAGTCAGCAGCTGATGCGAGAGCAGGTGAAGAAGGAGGAAGAGAAGCGGAAGGAAGAGTTTTCTGAGA
  AGGAAGCGAAGCTGCATTCTGAAATAAAAGAAAAGGAAGCGGGAATGAAGAAACACGAAGAAAACGAGGC
  TAAGCTTACTATGCAGGTCACAACATTAAATGAAAACTTGGGTACTGTGAAGAAGGAGTGGCAGTCTAGT
  CAACGGAGAGTCAGTGAGCTTGAGAAGCAAACGGATGACTTACGGGGAGAAATTGCTGTCTTAGAAGCAA
  CTGTTCAGAATAATCAAGACGAAAGAAGAGCACTCCTAGAGAGGTGTCTTAAAGGCGAAGGTGAAATAGA
  AAAGCTTCAGACCAAAGCCCTAGAGCTGCAGAGAAAGCTGGATAACACAACTGCGGCCGTGCAGGAGCTG
  GGCAGGGAGAACCAGTCTCTCCAGATCAAACACACACAAGCATTGAACAGAAAGTGGGCGGAAGACAACG
  AAGTGCAGAACTGCATGTCCTGTGGGAAGTGCTTCTCCGTCACCGTGCGGCGGCATCACTGCCGACAGTG
  TGGGAATATCTTCTGTGCCGAGTGCTCAACCAAAAATGCCTTAACTCCTTCTTCGAAGAAGCCTGTTCGT
  GTCTGTGATGCATGCTTCAATGATTTGCAAGGATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001001932
• Insert Size: 4236 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001001932.3, NP_001001932.1
• RefSeq Size: 7836 bp
• RefSeq ORF: 4236 bp
• Locus ID: 216238
• UniProt ID: Q8BL66
• Cytogenetics: 10 C2
• Gene Summary: Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.[UniProtKB/Swiss-Prot Function]