Akna (NM_001045514) Mouse Untagged Clone

CAT#: MC224410

Akna (untagged) - Mouse AT-hook transcription factor (Akna), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Akna
• Synonyms: AI597013
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224410 representing NM_001045514
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGCTCAGGGGCAAAGGCACAATGGGTTGGGCCAAGCCTGGGGCAAGGGCCCCGAAGGCGGCGCT
  GGGCTTGGGCTGAGGAACAAGATACAGATGGAAGAAGAGACCAGGGCTGGGGAAACAGCCAGTCTCTTCC
  CGAAGCCCCCAGTCCTGAGCTCCTAGAGGACTTCCGTCGGGCACAGGAGCACCTGCCACCCCTGGAGTGG
  GACCCAGACATGCAGGACTCCGAAGAGTCTTCTGGAGAAGAGACCGAAGCTGACGATGCAAGCAGCCCAG
  AGGGTTCCACCGTGCCTCTGCCCTGGCTCTCCAGGAGCAACCAACAGCTGGACATGTCTGAAGAGGAGCT
  GGATGAGGCCTCTGGGAGGCCTGAGGTAGACTTAGCTGGAGAGAGTTGTACGGAGCTTGAGTGTGAAGAC
  CAGGGAGATTCCAGCCCTCCACCTCCAGGACAGGGGCCAGCCAAAGGCTGGGTCACCTTTATCAAACAAG
  GCAGTAACTACAGACCTTCAGAGCATCTCGAGGCCCAGCCATCTGTTGAACATAGCCGCACAAAGTCTTG
  GAGCAGTGGAACTGTGAGCCTCCGTCAACCTAGTGACAGCCTTGGTTCTACCTGGGAAGGAGATACCGAA
  GTCCCTCAGCCTTCCATCTTGCCCAAAGCCCTGCCACAGAGTCCGTGCCACAACTTTCCACACCCAGGTG
  ACAGAAATGGAGGTGACGTTGCTCCGGCAACACCCACAGAATTCCGGGACTCCTTAGCAGCGCCAGCCCA
  GAATGCCGAGTGCTCTGCAGGCACGTGGGGGGAAGAAACCACCAGCCTACCCAGCTCTCGGCCTGAGGAC
  CAAACCTGGAAGAGGACTAAGACATCACCTAAGCCACTGCCCTCCCGGTTTACTGGTTCTGTCAGCCCCC
  TGAGTACTCGGCTTGGGGCAATAAAGAAAGTTGTGCCCCAGCACAAGCAGGGAGCCACTCTGGCTGGTCA
  CTCTTCTTCACAAGCTCCCAAGTATGGCAGGGGGCGCCGCCTGAACTACCCACTCCCCGACTTCTCCAAG
  GTGGGGCCCCGGGTGAGGTTTCCCAAAGATGAGAACTACCGACCCCCGAAGTCCAGAGGCCACAACAGGC
  AACAAGGCTCTACCAGACCCCTCATCTTCAAGTCGCCAGCTGAGATTGTTCGGGATGTACTGTTGAGTAG
  TGGAGAGGCGTCCCTGGCCAAGGAATCTTCTCTTGCTCACACCATCACCAGGGTGCCCCAGGAGTTCCAG
  ACACCCGAACAAGCCACTGAGCTAGTTCATCAGCTCCAGGAGGACTATCACAAGCTCCTCACCAAGTATG
  CGGAGGCTGAGAACACTATCGACCAACTTCGCCTTGGGGCCAAGGTGCACCTGTATTCAGACCCACCACA
  GCCCAGTCAGAGCTTCTGCTCGGGGTCAATGCCACAAGGAAGCAAGGTCTTATCCTTCTCTATCCCGCAA
  CCCAGAGTAGCAGAGTGGTGGCCTGACCCTGCCCAGGATCCACAGGCCTCTGAGGCCACAGGGTGGCCGT
  TCCCACGAACAGACCTGAGTCCCTCCTCGTCCCCCGGCGTGGCCACTCCAGGGCGGCTTCCTCAGAGCCA
  GGGCATTGCCACAGACCAGCCCTCCACAGGACAGACCCAGGCACTGACCTCTCAGGCCAGCCGGCTCCTG
  GCCAAGGTGCAGTCCTTCGAGGAACTGGTACTGGCTGGACACCTCCCACCTCAGGACCAAATCAAGAGCT
  TGGAGCAACTGAGGGCAGCCCACATGGCCCTAGAGGCAGAGTACCTGCAGGCCTGCAGAGAAGAGCTCCT
  GGACCCTCAGCTTGATGCCTCTCAGGGGAGTCCCAGGACCCTCAACCTCTGCAGGGAGCTAGAAGCAGAG
  ATATACCACCTGGGACAGCGCCTGGAAGAGCTACAGGACCATATGGACCAGACCCAAAGAGAGACAGAGC
  CATGTAGGCCAGACCTGCAAGACAGCACCCCAACCATGTCCTTTCTGCCCCAGTCAGCTCATCTGTCCAT
  GCCTTCAGGACCTGTCTCCTTGCCAGATGGCCAAACCTACCAGGAGCCTGCCACCACCACCACCACCTCC
  CCTGGAAGCTCTTGCACGCTCCCCATCAACAAGAAGTTAAGCCTGAGCATCAAAACTGAGGAAAGCCCAA
  GAGGTCTCCCGGTCCCACTCAGGGACAGGACGCTTCAGGTGGAGCAGGACTTCCATGGCCTTCTGGAGAG
  ATACCTCAGCGTTAAATCCCTCCCTGAAGCCTTGAGGGATGAAGATGAGGATGACCTGGAGGAGGAGGAA
  GAGGAGCAGGACCATCAAGGCCCCTTGGAAGTTGATAGTCCAGCCACAGCTCCAGGGAAAACAGAAGCCG
  TGAGGGTACCACCAGGAGAGCGCCCAACACAGGCTGAGGAAAGCCATAGGGATGCTACCCAGGAGGATGA
  GGAACAGATGGGACCTATGAAGTCACCAGACTTCCGGCCATCCATGGCCAGAGATACGTACACACCAGTC
  CTGGATACAGCTGAAGTGGCTCAACGGGGCACCAAAGCCATGGTGTCACATCAGAGCAGTCTGACCAGCC
  TGGAAGAGAGCAGGCCCTCAGAGCTCCTTCCTCGAAAAGCCCTGCTTCGGGCTGGTGGGCCCCACACTGA
  GGAGCCCTGGATGGTGTCACCAGAGACAGACAGTGGCTTCGTGGGCTCAGAAACCAGTATAGTGTCACCC
  TTCACCCAGACCCCAGAGCACCGGCTCTCCCATGTCAGCACTTCAGGACCATCAGCTCAACACCTCACTG
  CCTCTGTGCCTGGTGATAGAACCTCCCATCCCAAGGCCAGGGGTCTCATGGTTCCCAGAAGAGCCACTGA
  GACTGGCATACCCAGAAGCCGAACCCAGCAGCATTTCTCCAGCCTGAGCTCTCCCGGGAGAGGGGCACAG
  AGCTGCCACCTAGAGGAGACATCAGTGGCTAAAATAGCGGTTCCCAGGTCTGAGTTCAAGAGACAGAAGC
  AGATTTCCAAACAGCTCCTTCCCAGTGGAAGAACCAGCCCCGACTCTGCCCCAGCTCCCACAGCTGCCTC
  CACGCCCCATGGATCAGCAGAGAGCACTGCCAACCTCCTCCTCAACAGGACAGAGCGCGACCAGGCCATC
  AAGGATCTGCAGGCGGAGGTGTCACGGCTCCGCCTTCAACTGGAGGACAGCCTGCACCGGCCACACCCAG
  ACGGCCCAGCCTGTGTAGCTTCTGCTTTCAACCACTCCACCCAGACCCAGGAAAAGCTGGGTTCCTCACC
  CTCCTGGGGCCCCCACTATGGCAGTAAATCCACAGAGAGGCTGTCTAGGGAGCCTAATGGTGTAGAGCCA
  GCTGAGCCAATGGGAAGACGTCGAGCCAGGTCCTCTTCGGTGCCTCGGGATGTACCCAGACTGTACCTAA
  GTTCTGAATCGGAGTCGCCTGCCCCTCGGCTCTCATCTGAGAAGAGCAGGACCTTTGAGGAGCACCCCGA
  GGCTGCACAGTGGGGGACAAGACCACAGAGCAGCTCGAAGCGGAGGGAAAGAGTTTCCTTCCGGGGTCAA
  TACACAGGCCAGGAGTACCACATTCTATCCCCAAAGGCCATCCTGAAGGACAGTGGCACACCGTCCTGTC
  CCCACTGCCATCCCATTAGGACCCAGGACACAGGCAGTGCTGTCTCTAGAGACACCACACGGGGCTCATC
  TGCCGCTGACACCCTCCGCTGTGCCCTGTGTGGTGAAGTCAAGTCCTCTGCAGAAGCAGATGGCTCCAGC
  TCAGGTCCCTCTGAGAAGAACACCCCCAAAAAGCCTTCCACCCCCATCCTCAAACGGAAGAACAGGCAGA
  CGGGCTCGCCAGTACGAATGGCCCCTGGACTCTGGTACCTGGCTGCTGCGCCCCCAGCCCCAGCCCCACC
  GGCTTTGGCCTACATCTCCTCAGCTCCCATTATGCCTTATCTACCACCCACTGTGTACTATGCAGCCCCA
  GCACCTACCTCAGCCCAGACAGCTTCCCCGCAGCCTGCTCGGGGACCTCGGAGAACCCGGCACTCAGTCC
  AACTGGGTCTGAATGACCTGGAGGAGCTGCAGGCCGCACTCAGAGAGGCTGCACAGGCCGCCGAGAACGT
  TCGCTCCACCACCCGCCAGCTGAGCCGCTCCCTGTCTGCGGACCTGCGCCATGCCCGTAGCCTGCGAGGC
  TCCTGCCTTTTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001045514
• Insert Size: 4215 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001045514.3, NP_001038979.1
• RefSeq Size: 5480 bp
• RefSeq ORF: 4215 bp
• Locus ID: 100182
• UniProt ID: Q80VW7
• Cytogenetics: 4 B3-C1
• Gene Summary: Centrosomal protein that plays a key role in cell delamination by regulating microtubule organization (PubMed:30787442). Required for the delamination and retention of neural stem cells from the subventricular zone during neurogenesis (PubMed:30787442). Also regulates the epithelial-to-mesenchymal transition in other epithelial cells (PubMed:30787442). Acts by increasing centrosomal microtubule nucleation and recruiting nucleation factors and minus-end stabilizers, thereby destabilizing microtubules at the adherens junctions and mediating constriction of the apical endfoot (PubMed:30787442). In addition, may also act as a transcription factor that specifically activates the expression of the CD40 receptor and its ligand CD40L/CD154, two cell surface molecules on lymphocytes that are critical for antigen-dependent-B-cell development (By similarity). Binds to A/T-rich promoters (By similarity). It is unclear how it can both act as a microtubule organizer and as a transcription factor; additional evidences are required to reconcile these two apparently contradictory functions (Probable).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein.