D5Ertd579e (NM_001081232) Mouse Untagged Clone

CAT#: MC224407

D5Ertd579e (untagged) - Mouse DNA segment, Chr 5, ERATO Doi 579, expressed (D5Ertd579e), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: D5Ertd579e
• Synonyms: 9030221A05Rik; A930018H20Rik; Kiaa0232
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224407 representing NM_001081232
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCCCTGTCTGTACAGTTGTTGTGGATGGTTTGCCATCTGAAAGCACCTCAAGTTCTTATCCAGGCC
  CTGTGTCTGTTTCTGACATGTCGCTGCTTCATGCATTGGGCCCAGTGCAAACCTGGCTGGGACAAGAGCT
  GGAGAAATGTGGCATTGATGCCATGATTTATTCACGGTATATCCTCAGTCTTCTGCTGCATGACAGCTAT
  GACTACGACCTGCAGGAACAGGAGAATGACATCTTGAGCTGGGAGAAAGGAGCTTATAAGAAATGGGGAA
  GGAGTAAGAAGAAGTGTTCTGATCTAACGCTGGAGGAGATGAAAAAGCAGGCTGCTGTCCAGTGTCTGCG
  CTCTGCTTCTGATGAAAGCTCTGGGATCGAGACTTTAGTAGAAGAGCTCTGCTGCAGACTGAAAGACCTA
  CAGAGCGAGCAAGAAGAGAAGATTCACAAAAAGTTAGAAGGTTCTCCTTCTCCAGAGGAAGAATTGTCCC
  CTACAGCAAAAGATCAAGTGGAAATGTACTATGAAGCATTTCCACCACTGTCTGAGAAACCGGTTTGCCT
  GCAAGAGATCATGACCGTGTGGAACAAGTCCAAACCCTGTTCTTACTCTAGCTCCTCGTCATCATCCACA
  GTCCCACCAGCAAGCACGGATACATCTTCTCCAAAGGACTGCAACAGTGAGAGTGAAGCCGTCAGAGAGA
  GAAGCAGTGTCGCCTCTGTCCCCATGCATGAGAAAGCCCAGAGCAGGAGCAGACACGAGAAGGAGAGCAA
  GCTGAGCAGTAGCACCATCGAAGAAAAGCCTGCCTTTTACAAAAGGCAGATCCGACACAAACCTGAAGGG
  AAGACGCGCCCTCGCTCATGGTCATCTGGCTCTAGTGAAGCAGGTTCAAGTTCAAGTGGTAACCAAGGAG
  AGTTAAAAGCATCCATGAAGTATGTTAAAGTCAGACACAAGGCACGAGAAATTCGAAACAGAAAAGGGCG
  GAATGGGCAAAACAGGCATTCACTGAAGCATTGTGGAAAGGCAGAGAGAGGAGTCCATGCAGGCAGTGGT
  GGCAGCAGCAGCAGCAGCAGCAATGGGTCCATCAGGCAGCTGTGCAAGCGGGGTAAAAGACCAGCAAAGG
  AGACGGGAAGGAGCGACTCTGGGAACACTGCAGTGAAAGACCTGTATGTGGACAGCAGAAACAACAAAGA
  GTACAAGGAAGAGCCCCTGTGGTACACAGAGCCCATTGCTGAGTATTTTGTTCCTTTGAGTAGAAAAAGC
  AAACTAGAGACCACATACCGAAACAGGGAAGATACAAGTACTCTGACAGCAGAGGCAGTGGAAGACTTGT
  CTGACTCTGTGCGTGGTCTGTGTATCAGTAACAGTAATATCCACAGAACATACCTCGCAGCAGGTACTTT
  CATTGATGGTCATTTTGTAGAAATGCCTGCAGTTATAAACGAGGATATTGACCTCGCTGGGACCTCATTA
  TGTTCTCTACCAGAGGACAATAAGTATCTGGATGATATTCATCTATCAGAACTAACACACTTCTATGAAG
  TGGATATTGATCAATCCATGTTGGATCCTGGTGCCTCAGAAACAATGCAAGGAGAAAGTCGGATTTTGAA
  TATGATTCGACAAAAAAGCAAGGAGAATACAGATTTTGAGGCAGAATGTTGCATAGTGTTAGATGGTATG
  GAGTTGCAAGGGGAACGTGCAATATGGACAGATTCTACCAGCTCTGTGGGTGCTGAGGGCTTCTTCCTGC
  AAGACCTTGGCAATCTGGCTCAGTTTTGGGAGTGTTGTTCATCTAGTTCTGGTGATGCTGATGGAGAGAG
  TTTTGGAGGAGACTCTCCAGTTAGACTCTCTCCTATCTTGGACAGCACGATGCTCAGTTCACACATACTT
  GCTGGCAATCAAGAGCCCTTTTCCAATATTAATGAAGGATCTGGTATAAACTCTTGTTTTTCAGTATTTG
  AAGTGCAATGCAGTAATTCTGTTTTACCATTTTCTTTTGAAACACTCAACTTGGGAAGTGAACATGCAGA
  TTCTAGTGCTAATATGCTGGGGAAAACACAGTCTAGATTGCTCATATGGACCAAAAATAGTGCCTTTGAA
  GAAAACGAACACTGTTCTAATCTTTCAACAAGAACTTGTAGTCCGTGGTCCCATTCGGAAGAAGCACGCT
  CAGACAATGAGACACTAAACATTCAGTTTGAAGAATCGACACAGTTTACTGCAGAAGATATTAATTATGT
  GGTTCCCAGAGTCTCGTCAGATTTTGTAGATGAAGAGCTTCTAGATTTTTTGCAGGATGAAACTTGCCAG
  CAAAACAGTAGAACTTTAGGAGAAATTCCTACATTAGTTTTCAAAAAACGATCTAAGCTAGAATCTGTGT
  GTGGTATTCAGCTAGAACAAAAGGCAGAAAGCAAGAACTTTGAAACTACACATGCATGTAGTGAAAGCAG
  TCCACATGGAGATGGCTACAGCTCAGGGGTTATTAAAGACATTTGGACAAAGATGGTAGGTAGGAGTTCT
  GTGGCTGCAGTAGAGACAGAAAGAACTGGGGAGGAATTGTTTTCCACAGATGTAAATAACTACTGCTGCT
  GTTTGGATACTGAGGCGAAGATGGAAGCCCTTCAAGAGCCTAGTAGGGCTGTGCAGAGATCAGAGTATCA
  TCTCTGGGAGGGACAGAAGGAGAACATGGAGAAGAGAGCATTTGTCTCTAGTGAGCTGTCCAAGGTGGAT
  GGTGGCGATTACACCACACCCTCTAAACCTTGGGATGTGGCCCAAGATAAGGAGAACACATTCATTCTTG
  GAGGAGTTTATGGAGAGCTCAAAACATTCAACAGTGATGGGGAGTGGGCAGTTGTACCACCTGGTCACAC
  AAAAGGGAGCTTGCTACAGTGTGCAGCCTCTGATGTGGTGACAATCGCCGGTACAGATGTCTTTATGACC
  CCTGGAAACAGCTTTGCTCCTGGTCACAGGCAGCTCTGGAAGCCCTTTGTGTCATTTGAACAGAGTGACA
  TGCCGAAGAGCGGGGAAAATGGAGTAAACAAGGGGTTTTCTTTTATCTTCCATGAAGACTTGCTAGGAGC
  CTGCAGTAACTTTCAAGTTGAAGATCCTGGCCTTGAATACTCACTCTCTTCCTTTGACCTAAGCAACCCC
  TTCTCACAAGTTCTCCACGTAGAGTGTTCATTTGAACCAGAAGGGATTGCATCTTTTAGCCCCAGTTTCA
  AGCCGAAATCAATCCTCTGCTCTGATTCAGACAGTGAGGTTTTTCACCCCAGAATATGTGGTGTCGAAAG
  GACACAGTACAGGGCTATTCGAATCTCTCCCCGGACTCACTTTCGCCCGATTTCTGCATCTGAACTATCC
  CCTGGAGGAGGAAGTGAGTCAGAATTCGAATCTGAAAAAGATGAAGCGAGTGTTCCCATTCCTTCCCAAG
  TTGATGTATTTGAAGACCCACAAGCAGATCTCAAACCTTTAGAGGAAGATGCAGAGAAAGAAGGTCACTA
  CTATGGAAAGTTAGAGCTTGAGTCTGGGAAGTTCCTGCCCAGGTTAAAAAAATCTGGAATGGAGAAGAGT
  GCTCAGACATCACTGGATTCCCAGGAGGAGGCAACTGGGATTCTGCCGAAACAAAATCAGTGTTTGGAAT
  GTAACTTTAACGAATCTCTAGAAATTAATTTAGAAAGCTCAGCAGCAAACTGTAAAATAATGACACAATG
  TGAGGAAGAGATGAGTGAGTTTTGCAGTTGCAAAGCAGGTTGTCAGTTCCCTGCTTGTGAAGATAATCCA
  GTTTCTTCAGGACAATTGGAAGAGTTTCCTGTACTGAACACTGATGTGCAAGAAGTAACAAGAAATCAAG
  AAAAGCAGAGCTGGTGGGAAAAAGCTTTGTACTCTCCCCTTTTCCCCACGTCAGAGTGTGAAGAATGTTA
  TACAAATGCCAAGGGAGAGAATGGTATAGAAGAATGTCCAGATGCTAAAGAGACACCCAGTCGTGAAGAG
  CGTCTGTTAGATTTTAATAGGGTGTCTTCTGTTTATGAAGCAAGATGCACAGGAGAGAGAGGTTCTGAGA
  CAAAACCAAATGGCCTCCACAGGAAGATGTGTTCCAGTGCCAGCTCTGACACGGGCGACACAGGCTCCGA
  AGCCGGCGGAGAGTGGGTGGGCCCCAGCAGAGAGGAGCTCTTCTCCCGAACACATCTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001081232
• Insert Size: 4191 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081232.3, NP_001074701.3
• RefSeq Size: 6808 bp
• RefSeq ORF: 4191 bp
• Locus ID: 320661
• UniProt ID: Q80U59
• Cytogenetics: 5 19.18 cM