Pogz (NM_001165948) Mouse Untagged Clone

CAT#: MC224405

Pogz (untagged) - Mouse pogo transposable element with ZNF domain (Pogz), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pogz
• Synonyms: 9530006B08Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224405 representing NM_001165948
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGACACCGACCTGTTTATGGAATGTGAGGAGGAGGAACTGGAGCCATGGCAGAAAATCAGTGATG
  TCATTGAGGACTCTGTAGTTGAAGATTACAATTCTGTGGATAAAACTACTTCCGTTTCTGTGAGCCAGCA
  GCCAGTCTCAGCTCCAGTGCCCATTGCTGCCCATGCTTCTGTTGCTGGGCACCTCTCTACATCCACTACT
  GTTAGTAACAGTGGGGCACAGAACAGCGACAGTACAAAGAAGACTCTTGTCACACTAATTGCCAACAACA
  ATGCTGGTAATACTTTGGTCCAGCAAGGTGGACAGCCACTCATCCTCACCCAAAACCCAGCACCAGGTCT
  GGGAACAATGGTTACTCAACCAGTATTGAGACCTGTCCAGGTCATGCAGAATGCCAATCATGTGACTAGC
  TCCCCTGTGGCCTCACAACCTATCTTCATCACTACCCAGGGATTTCCTGTAAGGAATGTTCGGCCTGTAC
  AAAATGCAATGAATCAGGTTGGGATTGTGCTGAACGTACAGCAAGGCCAAACAGTTAGACCAATTACATT
  GGTCCCAGCCCCAGGTACACAGTTTGTTAAGCCGACAGTTGGCGTCCCACAAGTGTTCTCTCAGATGACC
  CCCGTGAGGCCAGGCTCCACAATGCCTGTGAGGCCCACCACCAACACCTTTACCACCGTCATCCCAGCTA
  CTCTCACCATCCGAAGCACTGTCCCACAGTCCCAGTCCCAGCAGACCAAGTCCACCCCCAGTACCTCCAC
  TACTCCCACTGCCACACAACCAACCTCACTGGGGCAGCTAGCTGGTCAGCCTCCAGGCCAGTCCAACCAG
  ACCTCGAATCCCAAACTAGTGAGCATTGCCAGCTTTGTCACTGTGAAGCGACCTGGGGTTACAGGTGAAA
  ATAGCAACGAAGTGGCCAAATTGGTGAATACTCTTAACACTGTCCCTTCCCTGGGCCAGAGTCCTGGGCC
  CGTGGTGGTGTCCAACAACAGTTCTGCTCAAAGAACCAGTGGGCCCGAATCTTCAGTGAAAGTGACCTCT
  TCCATCCCAGTGTTTGACCTCCAGGACGGTGGACGGAAAATATGTCCACGGTGTAATGCCCAGTTCCGTG
  TTACTGAAGCTCTGAGAGGTCACATGTGTTACTGCTGCCCTGAAATGGTTGAGTACCAAAAGAAAGGCAA
  GTCCCTCGATGCAGAGCCCAGTGTCCCATCGGCAGCAAAGCCCTCGTCCCCTGAGAAGACAGCCCCTGTT
  ACTTCCACACCCTCCTCTACACCAATTCCTGCTCTCTCACCACCTACCAAAGTACCAGAGCCAAATGAGA
  ATGCTGGAGATGCTGTCCAGACCAAGCTTATTATGCTGGTTGATGATTTCTACTATGGACGGGATGGGGG
  CAAAGCAGCCCAGCTCACGAGCTTCCCTAAGGTTGCCACGTCTTTCCGATGCCCTCATTGTACTAAGAGG
  CTAAAGAACAACATTCGATTCATGAACCACATGAAGCACCACGTAGAACTTGATCAACAGAATGGTGAGG
  TGGATGGTCACACCATCTGCCAGCACTGTTACCGCCAGTTCTCCACCCCTTTCCAGCTCCAGTGCCACTT
  GGAAAATGTTCATAGTCCCTATGAATCTACTACCAAGTGCAAGATCTGTGAGTGGGCCTTTGAAAGCGAG
  CCCTTGTTCCTCCAGCATATGAAGGACACCCACAAGCCTGGAGAGATGCCTTATGTTTGTCAGGTGTGTC
  AGTATCGCTCCTCCCTCTACTCTGAAGTAGATGTCCATTTTCGGATGATCCATGAAGATACTCGGCATTT
  GCTTTGCCCGTATTGCCTAAAAGTCTTCAAAAATGGCAATGCCTTCCAGCAACACTACATGAGGCACCAG
  AAGAGAAATGTTTATCACTGCAACAAATGCCGGCTGCAGTTTCTCTTTGCCAAGGACAAAATTGAACACA
  AGCTGCAGCACCATAAAACCTTCCGTAAGCCTAAGCAACTGGAAGGCTTGAAACCAGGCACCAAGGTGAC
  AATCCGGGCTTCCCGGGGGCAGCCGAGAACTGTTCCTGTGTCTTCAAATGATGCACCTTCTGGCACCTTG
  CAGGAGGCAGCAGCGCTGACCTCCACTGACCCCCTACCTGTCTTCCTTTATCCCCCTGTCCAGCGCAATA
  TCCAGAAGAGAGCTGTTAGGAAAATGAGCGTCATGGGCCGGCAGACATGTCTGGAGTGCAGCTTTGAGAT
  TCCAGATTTTCCTAATCATTTCCCTACCTATGTGCATTGTTCTCTCTGCCGATACAGCACCTGCTGCTCT
  AGAGCTTATGCCAATCACATGATCAACAATCATGTTCCACGGAAAAGTCCCAAGTATTTGGCTTTGTTTA
  AAAATTCTGTAAGTGGAATCAAGCTGGCCTGCACTTCGTGTACCTTTGCTACCTCCGTGGGAGATGCGAT
  GGCCAAGCATTTAGTGTTCAACCCCTCCCACAGATCTAGCAACATCTTACCACGGGGGCTCAGTTGGATG
  TCTCACTTGAGGCCTGGCCAGGCTTCTGAACGAGTGTTCGACTGGAGCATGAAGAATACATATCTTCCAC
  CTCCCCTCGTCCCTAACAAAGCTGCCACTGTGAAGCCTGTAGGAGTCACCCCAGCGGAGCCCCAAGAGCT
  GGCAGGTCCTGTGCTCCAGGCACTTCCATCCCCAGCCTCAACTGCAACCCCACCAGCAACCCCCACTCAC
  CCACAGCCCTCCGCCCTGCCACCCTCGGCCACAGAGGGGACTGAGTGTCTGAATGTCAGTGAACAGGAAG
  AAGGAAGCCCTGTCACCCAGGATCCTGAGCCAGCATCAGGTGGTGGAGGTGGCAGTGGGGTTGGCAAGAA
  AGAGCAGCTGTCTGTGAAGAAGCTTCGGGTAGTACTGTTTGCCCTGTGCTGCAACACTGAACAGGCGGCT
  GAACACTTTCGAAACCCCCAGCGGCGAATCCGTCGTTGGCTTCGACGCTTCCAGGCCTCTCAGGGAGAGA
  ACTTAGAGGGCAAATATCTCAGCTTTGAGGCAGAAGAAAAACTGGCTGAATGGGTGCTGATCCAGAGAGA
  GCAGCAGCTCCCTGTAAATGAGGAGACCTTGTTTCAGAAGGCCACCAAAATTGGACGCTCTTTGGAAGGG
  GGGTTTAAGATCTCCTATGAGTGGGCTGTGCGCTTCATGCTTCGGCACCACCTCACTCCCCATGCCCGGC
  GAGCTGTCGCCCACACGTTACCGAAGCATGTAGCAGAAAATGCTGGACTCTTCATTGAGTTTGTGCAGCG
  GCAGATTCACAATCAGGACTTACCTTTGTCTATGATTGTGGCTATTGATGAGATCTCTTTGTTCCTGGAC
  ACAGAGGTGCTGAGCAGTGATGACCGAAAGGAGAACGCTCTGCAGACAGTGGGCACAGGGGAGCCTTGGT
  GTGACGTCGTGCTGGCTATCCTAGCAGATGGCACTGTCCTCCCCACCCTTGTTTTCTTCCGAGGACAAGC
  GAATCGGTTCGCTAATGTGCCAGACTCTATATTACTAGAGGCCAAAGACAGTGGCTACAGCGACGACGAA
  ATCATGGAGCTTTGGTCAACCCGTGTATGGAAGAAGCACACAGCTTGTCAGCACAGCAAAAGCATGTTGG
  TGATGGACTGTCATCGCACTCACTTGTCAGAAGAAGTACTGGCCCTGCTCAGTGCCTCTAGCACTTTGCC
  TGCGGTAGTCCCCGCCGGTTGTAGCTCCAAAATCCAGCCACTAGATGTATGCATCAAACGAACTGTTAAG
  AACTTCCTGCACAAAAAGTGGAAGGAACAGGCTCGGGAAATGGCTGACGCTGCTTGTGATTCTGATGTCT
  TGCTTCAGCTGGTGTTGGTCTGGCTGGGAGAGGTGCTGGGTGTCATTGGGGACTCCCCAGAGCTAGTTCA
  GCGGTCCTTCCTTGTGGCTAGTGTTCTGCCAGGTCCCGATGGCAACGTTAACTCACCCACACGCAACGCT
  GACATGCAGGAGGAGCTCATTGCCTCCCTAGAGGAGCAGCTCAAGCTAAATGGAGAACAGTCTGAGGAGC
  ACTCAGCTTCTGCCCCCCGACCCAGGTCATCTCCTGAAGAGACAGTCGAGCCTGAAAGCCTTCACCAGCT
  TTTTGAGGGGGAAAGTGAGACGGAGTCTTTCTATGGCTTTGAGGAAGCTGACCTTGATCTGATGGAGATC
  TAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001165948
• Insert Size: 4203 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001165948.1, NP_001159420.1
• RefSeq Size: 7615 bp
• RefSeq ORF: 4203 bp
• Locus ID: 229584
• Cytogenetics: 3 F2.1
• Gene Summary: Plays a role in mitotic cell cycle progression and is involved in kinetochore assembly and mitotic sister chromatid cohesion. Probably through its association with CBX5 plays a role in mitotic chromosome segregation by regulating aurora kinase B/AURKB activation and AURKB and CBX5 dissociation from chromosome arms (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1, resulting in an isoform (2) that is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.