Tnik (NM_026910) Mouse Untagged Clone

CAT#: MC224337

Tnik (untagged) - Mouse TRAF2 and NCK interacting kinase (Tnik), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tnik
• Synonyms: 1500031A17Rik; 4831440I19Rik; AI451411; C530008O15Rik; C630040K21Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224337 representing NM_026910
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGAGCGACTCCCCAGCTCGCAGCCTGGATGAAATCGATCTCTCCGCCCTGAGGGACCCTGCAGGGA
  TCTTTGAGTTGGTGGAACTTGTCGGAAATGGCACGTATGGTCAAGTTTATAAGGGTCGTCATGTCAAAAC
  GGGCCAGCTTGCTGCCATTAAGGTTATGGATGTCACAGGGGATGAAGAGGAAGAAATCAAACAAGAAATT
  AACATGTTGAAGAAATATTCTCATCACAGGAACATTGCTACATACTACGGTGCTTTTATCAAAAAGAACC
  CTCCTGGCATGGATGACCAACTCTGGTTGGTTATGGAGTTCTGTGGTGCTGGCTCTGTCACTGACCTGAT
  CAAGAACACGAAAGGCAACACATTGAAAGAGGAGTGGATTGCATACATCTGCAGGGAGATCTTACGGGGC
  CTGAGTCACCTGCACCAGCACAAAGTGATTCATCGAGATATCAAAGGGCAGAACGTCTTGTTGACTGAAA
  ATGCAGAGGTTAAGCTAGTGGATTTTGGAGTGAGTGCCCAGCTTGACCGAACTGTGGGCAGGAGGAACAC
  GTTCATCGGGACTCCCTACTGGATGGCACCAGAAGTCATTGCCTGTGATGAGAACCCGGATGCCACATAT
  GATTTCAAGAGTGACTTGTGGTCTTTGGGAATCACCGCCATTGAGATGGCAGAAGGTGCCCCCCCCCTCT
  GTGACATGCATCCCATGAGAGCCCTCTTCCTCATCCCACGGAACCCTGCACCTCGGCTCAAGTCTAAGAA
  GTGGTCAAAAAAATTCCAGTCATTTATCGAGAGCTGCTTGGTAAAGAATCACAGCCAGCGGCCAGCCACG
  GAGCAGTTGATGAAGCACCCATTCATACGAGACCAACCTAATGAGAGGCAGGTCCGCATCCAGCTGAAGG
  ACCACATTGATCGAACAAAGAAGAAGCGAGGAGAAAAAGATGAGACTGAGTATGAATACAGCGGAAGTGA
  GGAAGAAGAGGAAGAGAATGACTCTGGGGAACCCAGCTCCATTCTGAACCTACCAGGGGAGTCAACACTG
  CGAAGGGACTTCCTGAGACTGCAGCTGGCCAACAAGGAGCGCTCAGAGGCCCTGCGGCGCCAACAGCTGG
  AGCAGCAGCAGCGGGAGAATGAAGAACACAAGCGGCAGCTACTGGCTGAGCGCCAGAAGCGCATCGAAGA
  GCAGAAGGAGCAAAGGCGGAGGCTGGAGGAGCAACAAAGGCGAGAAAAAGAGCTTCGGAAACAGCAGGAG
  CGGGAACAGCGCCGGCACTACGAAGAACAGATGCGTCGGGAGGAGGAGAGGAGGCGTGCCGAACATGAGC
  AGGAATACATCAGGCGGCAGCTAGAAGAAGAGCAAAGGCAGTTAGAGATCTTACAGCAGCAGCTACTGCA
  TGAGCAAGCTCTACTTCTGGAATATAAGCGCAAACAATTGGAAGAACAGAGACAAGCAGAAAGACTGCAG
  AGGCAGCTAAAGCAAGAGCGGGACTATCTGGTTTCCCTCCAGCATCAGCGGCAGGAGCAGAGGCCCCTGG
  AGAAGAAGCCACTGTACCATTACAAGGAGGGCATGAGTCCTAGTGAGAAGCCGGCCTGGGCCAAGGAGGT
  AGAAGAACGCTCAAGACTCAACCGACAGAGTTCACCTGCCATGCCTCACAAGGTTGCCAACAGGATCTCG
  GACCCCAACCTGCCCCCAAGATCAGAGTCCTTCAGCATTAGTGGGGTTCAGCCTGCAAGGACACCCCCAA
  TGCTCAGACCTGTTGACCCCCAGATCCCGCAGCTGGTAGCTGTCAAATCCCAGGGACCTGCCTTGACGGC
  CTCCCAGTCAGTACATGAGCAACCCACAAAGGGCCTGTCTGGGTTCCAGGAGGCTCTGAATGTGACCTCT
  CACCGGGTCGAGATGCCACGCCAGAACTCGGATCCCACCTCAGAAAACCCTCCTCTCCCCACGAGAATTG
  AGAAGTTTGACAGAAGCTCTTGGTTACGACAGGAAGAAGACATTCCACCAAAGGTGCCTCAAAGAACAAC
  TTCTATATCCCCAGCACTAGCCAGAAAGAATTCCCCTGGCAATGGCAGTGCTCTGGGCCCCAGACTTGGA
  TCTCAGCCCATCAGAGCAAGCAACCCTGATCTGCGCAGGACAGAGCCAGTCCTGGAGAGTTCCCTGCAGC
  GGACAAGCAGTGGCAGTTCCTCCAGCTCCAGCACTCCCAGCTCCCAGCCCAGCTCCCAAGGAGGCTCTCA
  ACCTGGCTCCCAAGCAGGATCTAGTGAGCGGTCCAGAGTGCGGGCCAACAGTAAGTCCGAAGGATCACCC
  GTGCTCCCCCATGAGCCTTCCAAGGTGAAACCAGAAGAATCCAGAGACATCACACGGCCCAGTCGGCCAG
  CTAGCTATAAGAAAGCTATAGATGAGGATCTGACGGCATTAGCCAAAGAATTACGAGAACTCCGCATTGA
  AGAAACAAACCGCCCCCTGAAGAAAGTGACTGATTACTCTTCCTCCAGCGAGGAGTCGGAGAGCAGTGAG
  GAAGAAGAGGAAGATGGAGAGAGTGAGACACATGACGGGACGGTGGCTGTCAGTGACATACCCAGACTAA
  TACCCACCGGAGCTCCAGGGAACAATGAGCAGTACAACATGGGGATGGTCGGGACACATGGGCTGGAAAC
  TTCGCATGCGGACACCTTTGGCGGCAGCATTTCAAGAGAAGGAACCTTGATGATCAGAGAGACGGCTGAA
  GAGAAGAAGCGATCTGGCCACAGTGACAGTAATGGATTCGCCGGTCACATCAATCTCCCAGACCTTGTAC
  AGCAGAGCCATTCGCCAGCTGGAACTCCCACTGAGGGGCTGGGCCGCGTCTCCACTCATTCCCAGGAGAT
  GGACTCTGGGGCTGAATATGGTATAGGGAGCAGCACCAAAGCCTCTTTCACCCCCTTCGTGGACCCTCGA
  GTGTACCAGACATCGCCCACTGATGAAGATGAAGAGGATGATGAGTCTTCAGCTGCTGCCCTGTTTACTA
  GCGAACTTCTTAGGCAAGAACAGGCCAAACTCAATGAAGCGAGGAAGATTTCAGTGGTAAATGTGAACCC
  AACAAACATTCGCCCTCATAGTGACACACCGGAAATCAGAAAATACAAGAAACGCTTCAATTCAGAAATA
  CTTTGTGCAGCTCTATGGGGTGTGAACCTTCTGGTGGGGACTGAAAATGGCCTGATGCTTTTGGACAGAA
  GTGGCCAAGGCAAAGTCTACAACCTAATCAACCGGAGGCGGTTTCAGCAGATGGATGTGCTAGAAGGACT
  AAATGTTCTTGTCACGATATCAGGAAAGAAGAACAAGCTCCGTGTGTACTATCTCTCATGGTTAAGAAAC
  AGAATCCTGCACAATGACCCAGAAGTGGAAAAGAAGCAGGGCTGGATCACTGTCGGTGACTTGGAAGGCT
  GCATCCATTACAAAGTCGTTAAATATGAAAGAATCAAGTTCCTGGTGATTGCCTTAAAGAATGCAGTAGA
  GATATATGCGTGGGCCCCTAAACCTTACCATAAGTTCATGGCATTTAAGTCTTTTGCAGATCTTCAGCAT
  AAGCCTCTGCTCGTTGACCTCACAGTAGAAGAAGGTCAAAGGTTAAAGGTCATATTTGGCTCACACACTG
  GTTTCCATGTAATTGATGTTGATTCTGGAAACTCCTACGATATCTATATACCATCCCATATTCAGGGCAA
  TATCACTCCTCATGCTATCGTCATCTTGCCTAAAACAGATGGAATGGAGATGCTTGTCTGCTATGAGGAT
  GAAGGGGTGTACGTGAACACCTACGGCCGGATCACTAAGGATGTGGTGCTCCAATGGGGAGAAATGCCCA
  CATCTGTGGCCTACATTCATTCCAATCAGATAATGGGCTGGGGCGAGAAAGCTATTGAGATCCGGTCAGT
  GGAAACAGGACATTTGGATGGAGTGTTTATGCATAAACGAGCTCAAAGGTTAAAGTTTCTATGTGAAAGA
  AATGATAAGGTATTTTTTGCATCCGTGCGATCTGGAGGAAGTAGCCAAGTGTTTTTCATGACCCTCAACA
  GAAATTCCATGATGAACTGGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_026910
• Insert Size: 4083 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_026910.1, NP_081186.1
• RefSeq Size: 7146 bp
• RefSeq ORF: 4083 bp
• Locus ID: 665113
• Cytogenetics: 3 A3
• Gene Summary: Serine/threonine kinase that acts as an essential activator of the Wnt signaling pathway. Recruited to promoters of Wnt target genes and required to activate their expression. May act by phosphorylating TCF4/TCF7L2. Appears to act upstream of the JUN N-terminal pathway. May play a role in the response to environmental stress. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. More generally, it may play a role in cytoskeletal rearrangements and regulate cell spreading (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes isoform 1.