Phlpp2 (NM_001122594) Mouse Untagged Clone

CAT#: MC224335

Phlpp2 (untagged) - Mouse PH domain and leucine rich repeat protein phosphatase 2 (Phlpp2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Phlpp2
• Synonyms: AI481772; C130044A18Rik; Phlppl
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224335 representing NM_001122594
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGAGGTGGAGCCCGTGCCCGCGGGCCCGCTGGAGCCCCCGGAGCCACCTGAAGCGGCGGCGCCTC
  GCCGGCCCGGAGGGATTCGGGTCCTAAAGAGAAATATGAAACACAATGGGAGCAGAACTTGTTTGAATAG
  AAGAAGTAGGTTTGGTTCCCGAGAAAGAGACTGGCTAAGAGAAGATGTGAAGAGAGGCTGTGTTTACCTT
  TATGGAGCAGACACGACCACTGCCACTACAACCACCTCCTCCTCCTCCTCCTCCTCCTCCTCTTCTGATT
  TACATCTTGTCCTTTGCACAGTAGAGACACCAGCGTCAGAAATATGTGCTGGAGAGGGAAGAGAAAGCCT
  CTATCTACAGCTTCATGGAGATCTGGTCAGGAGACTGGAGCCCTCTGAACGGCCTCTCCAGATTGTTTAC
  GATTACTTATCCAGGCTGGGGTTTGAAGATCCCGTGCGCATACAGGAGGAGGCTACGAACCCTGACCTCA
  GCTGTATGATTCGATTTTATGGTGAAAAACCATGCCAGATGGATCATCTGGATCGAATCCTACTGTCTGG
  CATCTATAATGTACGCAAAGGAAAAACCCAGCTGCACAAATGGGCTGAGCGCCTCGTTGTTCTCTGTGGT
  ACCTGCCTTATTGTTTCCTCAGTGAAGGATTGTCAAACTGGAAAGATGCACATTTTGCCGCTGGTTGGGG
  GAAAGATAGAAGAAGTGAAGCGCCGGCAGCACTCCCTTGCTTTCAGCTCAGCTGGAGCCCAAGCTCAGAC
  CTATCATGTCAGCTTTGAGACTTTGGCTGAGTACCAGAGATGGCAACGGCAGGCGTCTAAGGTGGTGTCG
  CAGCGGATGAGCACTGTGGACCTCTCATGCTACAGCCTTGAAGAGGTTCCTGAGCATCTCTTCTACAGCC
  AAGACATCACCTACCTCAACCTGCGGCACAACTTCATGCAGCTAGAAAGACCAGGGGGCCTTGACACACT
  CCACAAATTTTCTCAACTGAAGGGCCTGAACTTGTCCCATAATAAACTTGGACTGTTTCCTGTGCTGCTG
  TGTGAGATTTCTACCCTGACTGAGCTCAGCCTTTCTTGTAATGGATTTCATGACTTGCCAAGTCAGATTG
  GTAAATTGCTAAATCTTCAGACCCTCAGCCTCGACGGCAACGGCCTCACGGCTCTGCCAGATGAGTTGGG
  AAACCTGCGGCAGCTCACTTCTCTGGGAATTTCCTTCAACGATTTTCGTCACATCCCTGAAGTTTTGGAG
  AAACTTACCATGTTAGATAAAGTGGCTATGGCAGGAAACCGTTTGGAGGTCCTGAACCTGGGGGCACTGA
  CCAGGATGAGCCAGGTCAAGCACGTGGATCTGAGGATGAACCACTTGAAAACGGTGATTACTGAAAATAT
  GGAAGGAAATAAACATATCACGCACATGGATTTGCGGGACAATCAGTTGACTGATTTGGACCTTAGCTCC
  TTATGTAGCTTGGAGCAGCTGCACTGTGAGCGGAACCAGCTGAGGGAGCTGACACTCAGCGGCTTCTCCC
  TTCGAACCCTCTACGCCAGTTGGAACAGGCTGACGGCAGTGAATGTGTACCCAGTGCCCAGCCTGCTCAC
  TTCTTTGGAGCTCTCCCAAAACTTGCTGGAGTGTGTCCCTGACTGGGCCTGTGAAGCAAAAAAGCTGGAA
  ATACTAGACATAAGCCACAATCTTCTTACAGAGGTCCCCATGAGAATTCTGAGTAGTCTGAGTCTTCGGA
  AACTGATGGTAGGACACAATCACATCCATGTCCTTCCTGCACTGGTGGAGCACATTCCCCTCGAGGTCCT
  GGATATCCAGCATAACACTCTCTCCAGGCTGCCGGACACGCTCTTCTCCAAGGCCTTAAATCTCAGGTAC
  TTGAATGCATCTGCAAATAGTCTGGAGTCCTTGCCTTCTGCGTGTGCTGGAGAGGAGAGTCTGAGTGTGC
  TGCAGCTGCTGTACCTGACCAGCAACCTTCTGACAGATCAGTGCATACCAGTTTTGGTCGGGCACCCACA
  CCTGCGAGTTCTGCACCTTGCAAACAACCAGCTCCAGACCTTCCCTGCAAGCAAGCTAAATAAACTGGAG
  CAGCTGGAAGAGCTGAATCTAAGTGGGAACAAACTTACAGCTATCCCCACAACCATAGCCAACTGCAAAA
  GGCTGCACACCCTTGTCGCCCATGCCAACAACATCAGCATCTTCCCAGAGATCCTGCAGCTGCCTCAGAT
  TCAGTTTGTAGACCTGAGTTGCAATGACTTGACAGAAATCCTAATTCCAGAAGCTCTGCCTGCTACTTTA
  CAAGACCTTGATCTGACAGGAAATACAAATCTGGTCCTGGAACATAAGACTTTGGACATGTTTAGCCACA
  TCACAGCCCTGAAGATTGATCAGAAGCCTTTGCCAGCCACAGACTCTGCAGTTACATCAACCTTCTGGAG
  CCATGGCCTGGCCGAGATGGCAGGGCAGAGGAATAAACTCTGTGTGTCTGCCCTGGCTATGGATAACTTT
  GCAGAGGGTGTGGGAGCTGTCTACGGCATGTTTGATGGGGACCGGAATGAAGAACTCCCTCGTCTGCTGC
  AGTGTACAATGGCAGATGTGCTTTTGGAAGAAGTACAGCACTCAACAAATGACACAGTTTTCATGACCAA
  TACCTTCTTGGTATCTCACAGGAAATTAGGAATGGCTGGCCAGAAGCTGGGTTCTTCGGCTCTCCTGTGC
  TATATTCGACCTGACACTGCCGACCCAACAAGTAGCTTTAGCTTGACTGTAGCCAATGTTGGCATGTGCC
  AAGCTGTCCTGTGCCGAGGTGGGAAGCCAGTACCTCTTTCTAAAGTCTTCAGCTTGGAGCATGACCCAGA
  GGAGGCTCAGAGGGTGAAAGACCAGAAAGCCATCATAACAGAGGACAACAAGGTGAACGGAGTAACCTGC
  TGCACCCGGCTGCTGGGGTGCACATACCTCTACCCATGGATCCTCCCCAAGCCCCACATAGCTTCCACTC
  CACTTACCATTCAAGATGAATTGCTGATTTTGGGAAACAAAGCACTGTGGGAACACTTGTCATATCTAGA
  AGCTGTCAATGCAGTACGTCACGTGCAAGACCCACTAGCTGCTGCTAAGAAGCTGTGCACATTAGCCCAG
  AGCTACGGTTGTCAGGACAACGTGGGGGCAATGGTGGTTTATTTGAATATTGGTGAAGAAGGCTGTACCT
  GTGAAATGAATGGGCTCACCCTTCCAGGCCCTGTGGGATTTGCTTCAACTGCAGCCCTCAAGGATACTCC
  CAAGCCAACCACTCCCTCCTCCAGTAGTGGGATTGCCTCCGAGTTCAGCAGTGAGATGTCCACCTCAGAG
  GTGAGCAGTGAAGTGGGCTCCACGGCCTCGGATGAGCACAACACTGTGGGCCTGGAGGCGAGCTTGCTGC
  CCAGGCCAGAGAGGCGCTGCAGTCTGCACCCAGCGTCCTCCGCAGGGGTGTTCCAGCGCCAGCCTTCTTG
  TGCTACTTTTTCCAGCAATCAGTCTGACAATGGCCTGGACAGTGACGATGACCAGCCTGTTGAAGGAGTC
  ATAACCAACGGCAGCAGGGTTGAAGTAGAGGTAGACATCCATTGCTGCCGAGGCAGAGAGCCAGAGAGCT
  CCCCTCCGCTCCCGAAGAACTCTTCCAACGCCTGTTCAGAGGAGCGTGCTAGAGGGGCGGGGTTTGGGAT
  CCGAAGACAGAACAGTGTCAACAGTGGTATACTTCTGCCAGCGAACAGAGACAAGATGGAGCTGCAGAAG
  TCTCCCTCCACTTCTTGTCTGTATGGAAAGAAACTCTCCAATGGCTCCATTGTGCCCCTAGAGGACAGCC
  TGAACCTCATTGAGGTGGCCACAGAAGCACCCAAAAGGAAAACTGGCTACTTTGCTGCCCCCACTCAGCT
  GGAACCAGAGGATCAGTTTGTCGTCCCTCGAGACCTGGAAGAGGAAGTGAAGGAGCAGATGAAGCAGCAC
  CAGGAGGGCAGGCCTGAGCCCGAGCCCCGGGGAGAGGAGCGGACAGAGCCCCTGGAGGAGTTTGATACAG
  CACTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001122594
• Insert Size: 4068 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001122594.2, NP_001116066.2
• RefSeq Size: 7854 bp
• RefSeq ORF: 4068 bp
• Locus ID: 244650
• UniProt ID: Q8BXA7
• Cytogenetics: 8 D3
• Gene Summary: Protein phosphatase involved in regulation of Akt and PKC signaling. Mediates dephosphorylation in the C-terminal domain hydrophobic motif of members of the AGC Ser/Thr protein kinase family; specifically acts on 'Ser-473' of AKT1, 'Ser-660' of PRKCB isoform beta-II and 'Ser-657' of PRKCA. Akt regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of Akt triggers apoptosis and decreases cell proliferation. Also controls the phosphorylation of AKT3. Dephosphorylates STK4 on 'Thr-387' leading to STK4 activation and apoptosis. Dephosphorylates RPS6KB1 and is involved in regulation of cap-dependent translation. Inhibits cancer cell proliferation and may act as a tumor suppressor. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Dephosphorylates RAF1 inhibiting its kinase activity (By similarity).[UniProtKB/Swiss-Prot Function]