Fgd5 (NM_172731) Mouse Untagged Clone

CAT#: MC224333

Fgd5 (untagged) - Mouse FYVE, RhoGEF and PH domain containing 5 (Fgd5), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Fgd5
• Synonyms: C330025N11Rik; mFLJ00274; ZFYVE23
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224333 representing NM_172731
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACAGAGCAGATTCACCAAAGCCACCACTTGCTCCCAAGCCAAAGGTTGCTACCAACCCTTATGCAC
  CGGCAGCCAAGTTTCCCCCTTCACAGAGGCCTGACAGCTTCCCCAGTCCCAACTCCATGTCCAGGGGCCC
  CAAGCCCCCTATCGCTCCTAAGCCCAGACTGACTGGCCCCAGTGAGTACCTGAACAACAGCCTCGGCAAA
  TGCAGCAATGGGAGGCTGCTCTGTGAGGACCGGGGCCTGTACGACGGACACCACTCCACCCTGAATTGCT
  TGGAGTTGGAGCCTGATGAGCAGTATATCATGGTTCCCAGGGCTCCACAGAAAGAAGATACTCCCGTGGA
  TGGGGCTACCGAGGAGCCGGGGTTTGAGGGGGAAGTCCAGGAGCATGGTACAGAGCAGACAGGAACTGAG
  GGGGACCTGGAAGCTCCAGATGAAGAGGCACCAAGTAGAGACAGTGAGGAAGGCATGGTCCACGCTCTGG
  AAGATGAAGACTGTGATCACGATCCAGAGACGGATGGGACCCCAACATCGCCAGATGAAGGGGCACCAAG
  CAGAGACAGTGAGGAAGGTGAGGAGGACTGTGATCAGGGCCCAGGTATGGAGGAGCATCCCATGAGTGAA
  GAGGAGGGAGAAGAGGAGGAGGTGAAGGAGCACGTGTACAACAGTGATAACAGGGCACCCTGGGATGGAG
  AGGAGCCCTTCCCCAATGAGGTCATTCTCACACATGTCCGCTCTCAGTCCCCTGAAGTTCCCTGTTGGGA
  GCCAGGCCCTCCTGAGACTCCTGGAGAGGCAGAAGAGGATTGTGAAGACATCTGTAACAACACAGAACCT
  GGGAAACCCAATCAGGACACTGGTCAGGACACAGAGGATGCCGGCATGGGATCCCCTGAGAGTGAGGTGT
  CCCCAGATGTCCAGGAGCAAGAGGCAGCAACGGACAACCCTGAGGTCTTTGAGGAGGACTCTGCAGATGC
  TGCAGAAGGTGAGGATCAGATAGAGCAGGAGGAACCACCCAATTGTGACGAGGAAGCCTATAATAGAGAT
  GCCGCAGCAGCCACCATGCAGGTGGGAGAGGACCTCGGAGAGGAGGGAGACCATGTGCAGGAGGACCCTG
  CTGAGGAAAGCTGCCAGATCATTCCCTTTGAGAGCGACAGTGTGGAGGAGGATTTCTCACCTACACTCAC
  AGAGAATCCCTATGAGATTTTCCCAACCGAGAGCACTTCCTTCTGCAATAACACCTATTCCCTTGACGAG
  TCAGCCAATGGGCACGAGCCAGTGTGCGAGATCTGTGTAGAGGAGGTTCCTGGTGTTGGCCCTCCACTTA
  ACCAGCATGATTCCCTGCCAGATGGATCTGGAGAGGACTCCCCGGTGGTCCCTGATGTGGTGGTCGTGCC
  AGAGAATGAGGGGCCCGTGGATGATGCACTCAGCAGTCCATACGTGATGGGAGTTGGCTTGCTGAGCCTT
  GGAGAGGGAGCGCAGTCAGACACCCAGGCTGCATCAGGCACTCTGAGTGGGTACAGTACATGGGAGGAAG
  GGGACTCTGAGGGAGGGCAGGTCCCAGTGGATAGGAAGAATATTGCCACAAGGGCCCGGCCTCACTCTGG
  GAAGGTGGCTGGTCATGTTCCAGAAACTGTTCTAGAAGAAACGGGACCAGAAACCTGTTCATCAGGCATG
  GGCATCAGAGATACCAGTGATGAAGTGAGGAAGATAGGTATATTGCCAGAGGGAAAGCCTCCCGAGTGTG
  TTCGGGCCTTGCCGGCCAAGCCCAGAGCATTTACTCTCTACCCAAGGTCCTTCTCTGTAGAAGGCCGGGA
  GAGTCCCCTGTCCATGTTCCGGGAGCCAGAGGGAGCCGGGCTGGACAGCCACCGTGTAAGGAGGAAAGAG
  GACAACCTCTCTCTGCCGGGCGCCATCGGCTCCTCCGGTAGCTTCTCACAGCGCAGCCACCTGCCTTCCA
  GTGGCACCTCCACACCATCCTCTGTGGTTGACATCCCACCCCCTTTTGACTTGGCCTGCATCACGAAGAA
  ACCCATCACTAAAAGCTCACCCTCACTCCTGATAGACGGAGACACCCTGGAAAAAGCCTCTAAGAAGAAG
  AAGTCCTCCTTCAAACGCTTCCTGGAGCTGACGTTCAGGAAGAAGACAGAGAGCAAGGTGCACGTGGACA
  TGAACCTGTCGTCTTCCAGGTCTTCCTCTGAGTCCAGCTACCATGGTCCAGCCAGGGTACTGGAACTTGA
  CCGCAGAAGCCTCAGCAACTCGCCCCAGCTCAAGTGTCGCACTGGAAAGCTCCGGGCCTCTGACTCCCCG
  GCCGCCCTCATCTTCTACAGGGACAGCAAGAGGAAAGGCGTCCCCTTCAGCAGGACGGTGTCCAGAGTGG
  AGTCCTTCGAAGACCGCTCCCGGCCGCCCTTTCTGCCTCTGCCCCTCACCAAGCCACGGTCCATCTCATT
  CCCCAATGCCGACACTTCGGACTATGAGAACATTCCAGCCATGAACTCAGACTATGAGAATATCCAGATC
  CCCCCTCGCAGGCCGGTGAGGACTGGCACTTTCACAAAGCTGTTCGAAGAACAGAGCCGAGCCCTGTCCA
  CCGCAAATGAAAATGACGGCTACGTGGACATGAGCAGCTTCAATGCCTTCGAGAGCAAGCAGCAGAGTTC
  AGAGCAGGAAGCTGAGAGCGCCTACACTGAGCCCTACAAGGTCTGTCCCATCTCAGCGGCTCCCAGAGAG
  GACCTCACATCAGACGAAGAACAAGGAAGCTCCGAGGAGGAGGACAGTGCTTCAAGAGACCCCAGCCTCT
  CACACAAGGGTGAAGGACAGTCTAGAGCCCTTGTCATCGCTCAGGAGCTGCTGTCTTCAGAGAAAGCATA
  TGTGCAGATGTTGCAGCACTTAAGCCTGGATTTCCATGGAGCCGTCCTGAGGGCCTTGGAGAACGTAGAG
  CAAGAGGGCAGAGAGCCACTGGCCCAGGAGGAGCTGCGGCAGGGCCTGCGGGAGCTCCCAGCTATCTGTG
  ACCTTCACCAGGGCATCCTGGAGAGCCTGGAGCAGAGGCTGGGGGATTGTGGGGAGGGCCAGCCGCAGGT
  GGCCGACATCTTCCTGGCCCAGGAACAGGAGTTCGAGCATCATGCCGCACACATCCTGCAATTTGACAGG
  TACCTGGGGCTGCTCGCTGAGAGCTGCCTGCTCTCACCCCGGCTAGCCACCACGGTCCGGGAGTTTGAGC
  AGAGTTCACAAGGGGGTGGCCAGAGCATGAAGCATCGTATGCTGCGTGTGGTCCAGCGCCTCTTCCAGTA
  CCAAGTGCTGCTCACTGATTATTTAAATAACCTGTGCCCGGACTCAGCAGAGTACGACAACACTCAGAGT
  GCTCTGACTCTCATCTCTAAAGTGACAGACCGTGCCAACGAAAGCATGGAACAGGGGGAAAACCTGCAGA
  AGCTGGTCCACATCGAGTACAGTGTACGAGGCCAAGGGGACCTCCTCCAGCCAGGAAGGGAGTTCCTGAA
  GGAAGGGACACTGATGAGGGTGAGAGGGAAAAGCCGACACCCCCGCCACCTGTTCCTGATGAATGACACA
  CTCCTGTACACACATCCCCAGAAGGATGGGAAGTACCGGCTGAAGAGCTCGCTGCCAGTGGCCAACATGA
  AGGTCAGCCGCCCTGTGATGGACAAAGTGCCCTATGCTCTGAAGATCGAAACTCCTGAGTCTTGTCTGAC
  ACTGTCTGCAAGCTCCTGCGCGGAGCGGGACGAGTGGCACTACTGTCTGAGCAGAGCCCTTCCGGAGGAT
  TACAAGACTCAGGCCCTGGCTGCCTTCCACCACAGTGTGGAGATCCGGGAAAGGCTGGGGATCAGCCTCG
  GTGAGAGGCTCCCCACCCTGGTGCCTGTCACTCATGCCATGATGTGCATGAACTGCGGCTGTGACTTCTC
  CCTCACCGTGAGGCGCCACCACTGCCATGCCTGCGGCAAGATTGTGTGCCGGAACTGCTCTCGAAACAAG
  TACCCATTGAAGTGCCTCAAGAACAGGATGGCCAAGGTCTGTGATGGCTGCTTCCGGGAGCTGAAGTTGA
  GGAATGGGCCTGTCCCAGGCTCCATGAGAGAGCGTCCAGTCAGCATGAGCTTCCCACTGTCCTCGTCCCG
  CTTTTCCTCGGGCAGCGCCTTGTCCTCTGTCTTCCAGAGTATTAGCCCCTCAACTTTCAAGAAGCAGAAA
  AAAGTCCCTTCGGCTCTGTCCGAGGTGGCCGCGTCAGGAGAGGGCTCTGCCATCAGCGGCTACTTGAGCC
  GCTGTAAGAGTGGCAAGCGGCGCTGGAAGAAACTCTGGCTCGTCATCAAGGGCAAAGTACTCTACACCTA
  CTTGGCCAGCGAGGACAAAGTGGCCATGGAGAGCATACCTCTGCTGGGTTTTACTATTGCCCCAGAAAAG
  GAGGAGGGCAGCAGCGAAGTGGGCCCTGTTTTTCATCTTTATCACAAGAAAACCCTGTTTTACAGCTTCA
  AAGCAGAGGACAGCAATTCTGCTCAGAGATGGATGGAGGCCATGGAAGATGCCAGTGTGTTATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_172731
• Insert Size: 4545 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172731.3, NP_766319.3
• RefSeq Size: 6023 bp
• RefSeq ORF: 4545 bp
• Locus ID: 232237
• UniProt ID: Q80UZ0
• Cytogenetics: 6 D1
• Gene Summary: Activates CDC42, a member of the Ras-like family of Rho- and Rac proteins, by exchanging bound GDP for free GTP. Mediates VEGF-induced CDC42 activation. May regulate proangiogenic action of VEGF in vascular endothelial cells, including network formation, directional movement and proliferation. May play a role in regulating the actin cytoskeleton and cell shape (By similarity).[UniProtKB/Swiss-Prot Function]