Msh6 (NM_010830) Mouse Untagged Clone

CAT#: MC224318

Msh6 (untagged) - Mouse mutS homolog 6 (E. coli) (Msh6), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Msh6
• Synonyms: AU044881; AW550279; GTBP; Gtmbp
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224318 representing NM_010830
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCGACAAAGCACCTTGTACAGCTTCTTCCCCAAGTCCCCGGCACTCGGCGACACCAAGAAGGCCG
  CGGCCGAGGCCTCACGCCAAGGTGCCGCTGCCTCTGGAGCCTCCGCTTCCCGCGGCGGGGATGCGGCCTG
  GAGCGAGGCTGAACCCGGGTCCCGGTCCGCGGCGGTATCCGCCTCGTCGCCGGAGGCAAAGGATCTCAAC
  GGAGGGCTGCGGAGGGCCTCCTCCTCCGCGCAGGCCGTGCCTCCCAGTTCTTGTGACTTCTCACCAGGTG
  ATTTGGTTTGGGCTAAGATGGAAGGTTACCCCTGGTGGCCTTGCCTAGTTTATAATCATCCCTTTGATGG
  AACGTTCATCCGGAAGAAAGGGAAATCTGTCCGTGTTCATGTACAGTTCTTTGATGACAGCCCAACAAGG
  GGCTGGGTTAGCAAAAGGATGTTAAAGCCATATACAGGTTCAAAGTCAAAGGAAGCCCAAAAGGGAGGTC
  ATTTCTACAGTTCAAAGTCTGAGATACTCAGAGCAATGCAACGTGCAGATGAGGCCTTAAGTAAAGACAC
  GGCTGAGAGGCTGCAGCTGGCAGTGTGTGATGAGCCTTCAGAGCCGGAGGAGGAGGAGGAGACAGAGGTG
  CATGAGGCTTATTTATCTGATAAGAGTGAAGAGGATAATTACAACGAGAGTGAAGAAGAAGCACAGCCCA
  GTGTTCAGGGGCCTAGGCGAAGCAGCCGGCAAGTCAAGAAGCGACGGGTCATCTCAGACTCGGAGAGTGA
  CATTGGTGGCTCTGATGTAGAATTCAAGCCAGACACTAAGCAGGAAGGAAGCAGTGATGACGCGAGCAGT
  GGAGTTGGGGACAGCGATAGTGAGGACCTAGGCACCTTTGGCAAAGGCGCTCCAAAGCGGAAGAGAGCCA
  TGGTTGCTCAGGGTGGACTTAGAAGGAAAAGTCTGAAGAAGGAAACAGGCTCAGCCAAACGAGCAACTCC
  CATTCTCTCAGAAACCAAGAGTACCTTGAGCGCTTTTTCTGCCCCTCAGAATTCTGAATCCCAGACCCAC
  GTCAGTGGAGGAGGTAACGACAGTAGTGGACCCACTGTTTGGTATCACGAAACTTTAGAATGGCTTAAGC
  CAGAAAAGAGAAGAGATGAGCACAGGAGACGCCCCGATCACCCTGAATTTAACCCCACCACACTGTATGT
  GCCTGAAGAGTTCCTCAATTCTTGTACCCCAGGGATGAGGAAGTGGTGGCAGCTCAAGTCTCAGAACTTT
  GACCTCGTCATCTTTTATAAGGTGGGGAAGTTTTATGAGTTGTATCACATGGATGCTGTTATTGGAGTCA
  GTGAGCTGGGGCTGATATTCATGAAGGGCAACTGGGCCCATTCTGGTTTTCCAGAGATTGCGTTTGGCCG
  GTTCTCGGATTCCTTGGTGCAGAAGGGCTATAAGGTAGCACGAGTGGAGCAGACCGAGACTCCAGAAATG
  ATGGAGGCGCGATGTCGGAAAATGGCACACGTGTCCAAGTTTGATAGAGTGGTGAGAAGGGAGATTTGCA
  GGATCATTACCAAGGGCACACAGACCTACAGTGTGCTGGATGGTGATCCCTCTGAGAACTACAGTAGGTA
  TCTTCTTAGCCTCAAAGAGAAGGAGGAAGAGACTTCTGGTCACACTCGTGTGTATGGTGTTTGCTTTGTG
  GACACTTCACTGGGCAAGTTTTTCATCGGCCAGTTTTCGGATGACCGCCACTGTTCCAGATTTAGGACGC
  TAGTGGCTCACTATCCTCCAGTACAAATTTTGTTCGAGAAAGGAAATCTCTCAACAGAGACCAAAACTGT
  CCTAAAGGGTTCACTGTCATCTTGTCTTCAGGAAGGTCTCATACCAGGTTCCCAATTTTGGGATGCCACT
  AAGACCTTGCGAACACTCCTTGAAGGAGGGTACTTTACTGGAAATGGAGACAGTAGTACAGTGCTGCCTC
  TGGTGCTTAAAGGAATGACCTCGGAGTCAGATTCTGTTGGGTTGACACCAGGCGAGGAGAGTGAATTGGC
  TCTCTCTGCTCTAGGTGGTATTGTTTTCTACCTCAAAAAATGCCTGATTGATCAGGAGCTTCTGTCAATG
  GCTAATTTTGAAGAGTATTTCCCTTTGGATTCTGACACGGTCAGCACAGTAAAACCTGGGGCTGTCTTTA
  CTAAAGCCAGTCAGCGGATGGTGCTAGACGCAGTGACATTAAACAACCTGGAGATTTTCCTGAATGGGAC
  AAATGGCTCTACTGAAGGGACCCTGCTAGAGAGATTGGATACTTGCCATACGCCCTTTGGCAAGCGGCTC
  TTAAAACAGTGGCTCTGTGCCCCCCTCTGCAGCCCTTCTGCCATCAGTGACCGTCTAGATGCGGTGGAAG
  ATCTGATGGCCGTGCCTGACAAGGTCACTGAAGTTGCAGACCTCTTAAAGAAGCTGCCAGACCTTGAGAG
  GCTACTGAGTAAGATTCATAATGTTGGGTCTCCCCTGAAGAGCCAGAACCATCCAGACAGCAGGGCTATA
  ATGTACGAAGAAACGACATACAGCAAAAAGAAGATCATTGATTTTCTCTCTGCTCTAGAAGGATTCAAAG
  TAATGTGTAAAGTCTCAGGGCTTCTGGAGGAGGTCGCGGGTGGTTTTACGTCCAAAACCCTCAAGCAGGT
  TGTTACTCTGCAGTCAAAAAGTCCTAAAGGCCGCTTTCCTGACCTGACTGCAGAACTGCAGCGATGGGAC
  ACAGCCTTTGACCATGAGAAGGCTCGGAAGACCGGACTCATCACTCCAAAAGCAGGGTTTGACTCTGATT
  ATGACCAAGCACTTGCTGACATCAGAGAGAATGAACAGAGCCTCCTGGAGTACTTAGACAAACAGCGCAG
  TCGGCTTGGCTGTAAGAGCATAGTCTACTGGGGAATCGGTAGGAACCGTTACCAGTTAGAGATTCCAGAG
  AATTTCGCTACCCGTAATCTACCTGAAGAATATGAGCTGAAATCTACTAAAAAAGGCTGTAAACGATACT
  GGACCAAAACGATTGAGAAGAAATTAGCTAATCTTATTAATGCTGAAGAACGCAGGGACACATCCTTAAA
  GGACTGCATGCGGCGCTTGTTCTGTAACTTCGACAAGAACCACAAGGACTGGCAGTCTGCTGTAGAGTGC
  ATTGCAGTGTTGGATGTCTTACTGTGCCTGGCTAACTACAGTCAAGGAGGAGATGGTCCTATGTGTCGCC
  CAGAAATTGTGTTACCAGGAGAAGACACACATCCCTTCTTAGAGTTTAAAGGGTCACGACATCCCTGCAT
  TACAAAGACTTTTTTTGGAGACGATTTTATTCCTAACGACATTCTAATAGGCTGCGAGGAAGAAGCAGAG
  GAACATGGCAAAGCCTATTGTGTGCTTGTGACTGGACCAAATATGGGGGGCAAGTCTACACTCATAAGAC
  AGGCTGGTCTGTTGGCTGTGATGGCCCAGCTGGGTTGTTACGTACCTGCTGAGAAGTGCAGGCTCACACC
  AGTTGACAGAGTGTTTACTAGGCTTGGTGCCTCAGATAGAATAATGTCAGGAGAAAGCACATTTTTTGTT
  GAGTTGAGTGAAACTGCTAGCATACTCAGGCATGCAACAGCACATTCTTTGGTACTTGTGGATGAATTAG
  GAAGAGGCACCGCAACTTTTGATGGGACAGCGATAGCCAATGCGGTTGTTAAAGAACTTGCTGAAACCAT
  AAAGTGTCGAACACTGTTTTCTACACACTATCATTCATTAGTAGAAGATTATTCTAAAAGTGTTTGTGTG
  CGCCTAGGACATATGGCATGCATGGTAGAAAATGAATGTGAGGACCCCAGCCAGGAGACTATTACTTTCC
  TCTATAAATTCATTAAGGGAGCTTGTCCTAAGAGCTATGGCTTTAATGCAGCAAGGCTTGCTAATCTCCC
  GGAGGAGGTTATTCAGAAGGGACACAGAAAGGCAAGAGAATTTGAGAGGATGAATCAGTCATTACAGCTA
  TTTCGGGAAGTTTGTCTGGCTACTGAAAAGCCGACTATAAACGGTGAAGCTATCCATAGGCTGCTGGCCT
  TGATTAACGGATTGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_010830
• Insert Size: 4077 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010830.2, NP_034960.1
• RefSeq Size: 4288 bp
• RefSeq ORF: 4077 bp
• Locus ID: 17688
• UniProt ID: P54276
• Cytogenetics: 17 57.87 cM
• Gene Summary: Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. Recruited on chromatin in G1 and early S phase via its PWWP domain that specifically binds trimethylated 'Lys-36' of histone H3 (H3K36me3): early recruitment to chromatin to be replicated allowing a quick identification of mismatch repair to initiate the DNA mismatch repair reaction (By similarity).[UniProtKB/Swiss-Prot Function]