Arhgef10 (NM_172751) Mouse Untagged Clone

CAT#: MC224314

Arhgef10 (untagged) - Mouse Rho guanine nucleotide exchange factor (GEF) 10 (Arhgef10), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Arhgef10
• Synonyms: 6430549H08Rik; mKIAA0294
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224314 representing NM_172751
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCAGGGAGAGGCCTCACCTCCTGTTCCTGCAGAACATGAAGCGAAATGTGACACCAGCAACAACG
  AAGAGGAGGGAGAACTGTTTGACTTCGACAGTGGAGATGAAGTTCCCGAAGCGGATCGGCAAGTCCCGTC
  TGCCGACGATAGGACGAGAGGTGCTGAAGCAGGAGGTGCAGACGAGAACACGTGCCCTGCAGGAAATGGA
  ACTGGAGCTGAACCTGCCCCCGAGGCAGAGCCTGCGAAACTTGTGGTCCCCACAAAAGTGAACCCGTATT
  CTGTCATCGACATCACGCCGCTGCAGGAAGACCAGCCGTCATCTCCAGACGCGAATACAGAGGAGGAAGG
  CGTGGGGCTGCGTGTGCCCAGTGGTTACTCTGTGCCTGTGCCCTGTGGCTATGCTGTCCCCTCCAACCTG
  CCATTGCTGCTGCCTGCCTACTCCAGTCCTGTCATCATCCGAGCCGAGTCTGTAGAGGAGGAAGAAGCAG
  CAGAAACTGTGGGAGATGGGCAGTGCAATTCCCTAAGCTCCGAGGACCTTCCACACAGCAGTGAGCAAGG
  CAGCCAAGAAGGCAGTGCCCTGGCCCGATGGGCAGCCGACCCTGCCAACACAGCATGGATGGAAAATCCA
  GAGGAAGCAATTTACGACGACGTTCCGAGGGAAAACTCGGACTCTGAGCCAGATGAAATGATTTATGATG
  ATGTGGAGAACGGCGAAGAAGGTGGAAACAGCTCCCCAGAGTACGGGTGGAGTTCCAGCGAATTCGAAAG
  CTATGAGGAGCCAAGCGACTCGGAGGGCAAGAACGGAATTCCCAGGTCCTTCCTGCGCAGCAGTCATAAA
  AAGCAACTCTCTCATGACCTAACCCGCTTTAAGGCGCACTGTGAGGAAAAGATGAGAGGCTTGGTGGCCA
  GTACGGTGGGAGCCATGGAGATTCAGCAGGCAAAGCAGAGGCAGGAGCGGAAGATGCAGAAGCTTATGAA
  GGCAGCCAAGGAGGGCACCAAAGATGGGCTGGAGAAAACCAAAGCTGCTGTGAAGAGAGGCGGCTCCTTC
  ATCAGGACCAGGTCCTTGGTCTCTCAAGATCACAGGTGTTATTTTGAAGAAGAGCAGAATTTGTTCATTG
  ACGTGGACTGCAAGCACCCGGAGGCTGTCCTCACCCCAATGCCCGAGGGCTTGTCTCAGCAGCAGGTTGT
  GAGAAGATACATTCTGGGCTCCATTGTTGAGAGCGAGAAGAACTATGTAGATGCTCTCCGGAGGATTTTG
  GAGCAATATGAGAAGCCGCTGTCTGAGATGGAGCCCCGGCTCCTGAGTGACAGGAAGCTCAGGATGGTTT
  TCTACCGCGTCAAGGAGATCCTGCAGTGTCACTCCATGTTCCAGATTGCGCTGGCCAGCCGCGTGTCCGA
  GTGGGATGTCGTGGAAACAATCGGCGACGTTTTTGTGGCCTCGTTCTCCAAGTCCATGGTGCTGGATGCT
  TACAGCGAGTACGTGAACAACTTCAGCACGGCCGTGGCTGTCCTCAAGAAGACGTGTGCCACAAAGCCTG
  CTTTCCTTGAGTTCTTAAAGCTGAGCCAGGATTCCAGTCCAGACCGAGTCACACTCCACAGCCTTATGAT
  GCGGCCCATCCAGAGGTTCCCACAGTTCATCCTCCTACTGCAGGACATGCTGAAGAACACCGCCAAAGGA
  CATCCTGACAGGCTGCCCCTTCAGATGGCGCTGACGGAGCTCGAGACCCTGGCAGAGAAGCTAAACGAGA
  GAAAAAGAGATGCCGACCAGCGATGCGAAATCAAACAAATAGCAAAAGCCATAAACGAGAGATACCTGAA
  CAAGCTCCTCAGCAGTGGAAACCGGTACCTCGTCCGGTCCGATGACGTCATCGAGACGGTTTACAACGAC
  CGGGGAGAAATCGTCAAGACCAAACAACGCCGGATCTTCATGCTGAATGACGTGCTCATGTGTGCCACCG
  CCAGCTCACGGAACTCACATGAAAGCCATGCCGTGATGAGCCAGAGGTACCTGCTCAAGTGGAGTGTCCC
  TCTAGGTCACGTGGATGTGATTCAGTACAATGGAGGCTCTGGCGCCGGGGAGCACTGTCGGCACCACGCT
  GCACACTCACCTGAGAGCCTGGCCGTGGTGGCCAACGCGAAGCCACACAAAGTTTACATGGGGCCAGGGC
  AGCTGTACCAGGATCTGCAGAACCTACTGCACGACCTGAACGTTGTTGGCCAGATCTCGCAGCTGATAGG
  AAACCTCCGAGGGAGCTATCAGAACTTAAACCAGTCTGTAGCCCATGATTGGACGTCGGGCTTACAGCGG
  CTGATACTGAGGAAAGAAGATGCGATCAGAGCTGCCGACCGCTGCCGGATCCAGTTACAGCTCCCGGGAA
  AGCAGGACAAGTCCGGGCGGCCCACGTTCTTCACTGCTGTGTTCAATACGCTTACACCAGCCATCAAGGA
  GTCCTGGGTCAGCAGCCTGCAGATGGCCAAGCTGGCCCTTGAGGAGGAGAACCATATGGGATGGTTCTGT
  GTGGATGACGATGGCAACCTAGCCAAGAAGGAGACACACCCTCTCCTGGTGGGACACATGCCTGTGATGG
  TGGCCAAGCAGCCAGAGTTCAAGATTGAATGTGCAGCTTATAACCCTGAACCTTACCTGAGCAATGAAAG
  TCAACCAGCCTCACCCTCCACAGCCCGTGGCTTTCTGTGGATTGGAAGCTGTAGCAACCAGATGGGTCAG
  GTCGCCATTGTCTCCTTCCAAGGTTCCAACCCCAAAGTCATCGAGTGCTTCAATGTGGAATCACGCATCC
  TGTGTATGGTATACATTCCCGCAGAGGAGTCAAAGCCCCAAGAAACCACCGAAACCAAGGATCCAGAAGC
  CACAGCCTCGAGAGCACCCCATGTGCCCACCATCTGCCTGGGCACAGAAGAGGGAAGCATTTCCATTTAC
  AAAAGCAGTCAGGGCTGCAAGAAGGTGAGGCTTCAGCACTTCTATGCACCGGACAAGTCCACGGTCATGA
  GCTTGGCCTGCTCCCCACAAGGCCTGTATGCAGGCCTGGTCAATGGGTCCGTAGCCAGCTACACCAAGGC
  TCCAGATGGATCCTGGAACTCGGAACCCCAGCAAGTGATCAAGTTGGGTGTCCTGCCAGTCAGAAGCCTT
  CTCCTGGTGGAGGGTGCACTGTGGGCAGCCTCGGGAGGGCAGGTGTTCATGGCCAGCGTGGAGACTCACA
  CTATAGAGAATCAGCTGGAGGCCCACCAAGATGAAGGGATGGTCATCTCCCACATGGCTGTGGCTGGCGT
  TGGCATCTGGATTGCCTTCACCTCGGGGTCCACCCTCCGCCTCTTCCACACAGAGACCTTGAAGCACCTG
  CAGGATGTCAACATTGATGCCCCTGTGCATAGCATGCTGCCAGGGCACCAGCGTCTATCTGTGACCAGCC
  TGCTCGTCTGCCACGGCTTGCTCATGGTCGGTACCAGCCTGGGTGTCGTGGTGGCCCTGCCAGTCCCTCG
  GCTTCAGGGTATCCCCAAAGTGACAGGGCGAGGCATGGTCTCCTACCATGCGCATAACGGGCCCGTTAAG
  TTCATCGTCTCAGCCACGGCGTTTCAAAACAAGGACAGAGCCAGGGACAGCCCACGTTCCGGTTCTGAGC
  TTCAGGATGAAGACCCCAAGGACTTGCTCTGCAGCGAGGAAGGTCCTTCCTGCCCGGGCCAGCCTGACAC
  CTACACATCCGTTTGGTTGGGAGACTCCCTAGGATTGCCAACTCAAAAGAATGATCTGTCTTCGTCCTCG
  GGGTCACTGAACCTGTCCCATGGCTCCAGTTCCCTAGAGCACAGATCTGTGGACAGCAGTCTGTGTGACC
  TGCTGAGGGACCCCAGTGCCTCCCCAAGGAGCAGACCACAGGGCTCTAGGCGAGCAAGAGCCAGTTCAGC
  GCTGGTGGTATGTGGGGGCCAGGGCCACCGGCGGGTGCACAGGAAGGCCAGGCAGCCTTCTCAGGAGGAC
  CTGGTTTCTTCAGTGATGGTGTGGCAGATCCCCCTGCTGGGCATGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_172751
• Insert Size: 4038 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172751.3, NP_766339.2
• RefSeq Size: 5541 bp
• RefSeq ORF: 4038 bp
• Locus ID: 234094
• UniProt ID: Q8C033
• Cytogenetics: 8 A1.1
• Gene Summary: May play a role in developmental myelination of peripheral nerves.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform a).