Adgrf5 (NM_001081178) Mouse Untagged Clone

CAT#: MC224313

Gpr116 (untagged) - Mouse G protein-coupled receptor 116 (Gpr116), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Adgrf5
• Synonyms: 8430401C09Rik; 9330185D23; AI528491; Gpr116; mKIAA0758
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224313 representing NM_001081178
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGATCGCCAAGGACCTTCACGTTCTACTTCCTGCTTCTTGTGATTTGTTCTTCAGAAGCTGCGCTGA
  GCACACCAACAGAGCCCATTGTTCAACCTTCAATTCTCCAAGAACATGAGCTAGCCGGGGAAGAGCTACT
  GAGGCCAAAAAGAGCAGCTGCAGCGGGTGATCGCGTGGCGGAAGAATACATGGTGGATATTGAGATCAGC
  TTTGAAAATGTCTCCTTCCTGGAGTCCATCAGAGCTCACTTGAACAACCTCAGTTTTCCAATTCGAGGGA
  CCGAAGCTGACATTTTGAACATAGCAATGACTACAGTCTGCACTCCTGCTGGAAATGACCTCTTGTGCTT
  CTGTGAGAAAGGCTACCAGTGGTCTGAGGAAAGGTGTCTCCACTCTCTCACTTGTCAAGACTATGACAGT
  GCCCTGCCAGGCGGCTACTGCAGTTGTCTGAAAGGACTTCCTCCCCAGGGACCTTTCTGCCAGCTCCCAG
  AAGCATTCATTACCTTAAAACTCAAAGTCAGACTGAACATAGGATTTCAAGAAGACCTAAAGAACACGTC
  TTCTGCCCTCTATAGGTCCTACAAGACTGATCTGGAAAGAGCGTTCCGAGCGGGTTACAGAACTTTACCA
  GGATTCAGATCGGTGACTGTGACACAGTTCACCAAGGGCAGCGTGGTTGTGAACTACGTAGTCCGGGTTA
  CATCGGCACCACTGCCTGGATCTATTCATAAAGCCAATGAACAAGTCATACAGAACCTCAACCATACTTA
  CAAAATGGACTACAACTCCTTCCAGGGAACCCCGAGCAATGAGACGAAATTCACTGTCATACCAGAATTC
  ATCTTTGAGGGAGACAATGTGACTCTGGAGTGTGAAACTGAGTTCGTGACCTCCAACACGTCTTGGTACT
  ATGGAGAAAAACGCTCTGACATCCAGAACAGCGACAAATACTCCATTCACACCACTGTCATCAACAATAT
  AAGCCTGATCACCCGCCTTACCATTTACAACTTCACACAGCATGATGCGGGCATGTATGGTTGCAATGTG
  ACATTGGATATTTTTGAATATGGAACAGTCAGAAAATTAGATGTCACTCCCATCCGGATTTTGGCCAAGG
  AGGAGAGAAAGGTGGTGTGTGACAACCATCCTATATCGCTGAACTGCTGCAGCGAGAATATTGCAAACTG
  GAGCAGTATTGAATGGAAACAGGAAGGGAAGATAAGCATTCTAGGGAACCCAGAATCAGACCTAGAGTCT
  AGCTGCAGCACTTACACGCTCAAGGCAGATGGAACCCAGTGTCCCAGTGGGTCTTCTGGAACAACAGTCA
  TCTACACATGCGAGTTCGTCAGTGCCTATGGAGCCAGAGGCAGTAAGAACATAGCTGTGACCTTCACCTC
  TGTAGCCAACCTAACAATAACCCGGGACCCAATTTCTGTTTCTGAGGGACAAAGCTTTTCTATAACCTGC
  CTCAGCGATGTGAGTAGCTTTGATGAGGTGTACTGGAACACCTCAGCTGGTATTAAAATCCACCCAAGGT
  TTTATACCATGAGGAGGTATCAGGATGGAGCAGAGTCGGTGCTGATGGTGAAGACCTCCACCCGAGAGTG
  GAACGGAACCTACCACTGCATATTTAGATATAAGAATTCATACAGCATTGCCACCAAAGATGTCACCGTT
  CACCCGCTGCCTCTGGTGTCAGACATCATGATGGATCCTTTGGAAGCCAGTGGTCTGTGTACCAGTTCCC
  ATCAATTCAAATGCTGCGTAGAAGAGGATGCAGGGGAAGAGTACGCTGTGACTTTCCACGTGGACTCCTC
  ATCCTTTCCCGCTGAAAGAGAAGTTATCGGCAAGCAAGCATGCTACACATACAGTCTCCCAGCGAACTTA
  CCTCGATGTCCCAAAGACATCGCAGTGTTCTGCCACTTCACCAATGCAGCCAACAGCTCAGTCCGGAGCC
  CATCTATGAAGCTCAAACTTATTCCAAGGGAGAATGTCACATGCCAGGATCCCATCATTGGCATTGGGGA
  TCCTGGGAAAGTCATTCAGAAGCTGTGCCAGTTCTCAGGTGTTTACGGAAGCCCTGGACAGGCCATCGGC
  GGGACTGTCACTTACAAATGTGTGGGCACCCAGTGGAAGGAGGAGTCAAGGGCCTGCATCTCAGCTCCAA
  TCAATGGTTTGCTCCAGGTGGCCAAGGCTTTGATCAAGAGCCCCACCCAGGACCAGAAGCTCCCTACATA
  CCTGAGGGACCTTTCTGTTAGTGCTGGGAAGGAAGAACAGGACATCCGCTCATCTCCAGGAAGCCTGGGA
  GCCATCATCAGCATCCTTGACTTGCTCTCAACGGTTCCCACCCAAGTGAATTCAGAAATGATGAGAGACA
  TACTTGCTACCATTAACGTCATCTTAGACAAGTCTGCCTTGAACTCCTGGGAGAAGTTGCTACAGCAACA
  GAGCAATCAGAGTTCTCAGTTCCTGCACTCCGTGGAAAGGTTTTCCCAAGCCCTCCAGCTGGGAGACAGC
  ACTCCTCCATTCCTCGCCCACCCTAACGTTCAGATGAAGAGCATGGTGATAAAACGTGGCCACCCCCAAA
  TCTACCAACAGCAGTTTATCTTCAAAGACTCTGACCTGTGGGGTGATGTAGCCATTGATGAGTGTCAGCT
  GGGAAACTTGCAGCCTGATTCATCGATCGTTACTGTGGCTTTCCCAACTCTCAAAGCCATCCTTGCCCAG
  GATGTACAGAGAAAGACATCCTCCAACAGCTTAGTAATGACAACCACTGTCAGCCATAACATTGTCAAGC
  CATTTAGGATTTCTATGACTTTTAAGAACAACCATCGTTCGGGTGGCAAGCCACAGTGTGTCTTCTGGAA
  CTTCAGTCTTGCCAACAATACAGGGGGCTGGGACAGCAGTGGGTGCTCTGTGGAAGATGATGGTAGGGAC
  AATAGGGACAGAGTCTTCTGCAAGTGTAACCACCTGACGTCATTCTCCATCCTCATGTCCCCAGACTCCC
  CAGATCCAGGGTCTCTTTTAAAAATACTTCTGGATATCATTTCTTACATCGGGTTGGGGTTTTCCATAGT
  CAGCTTAGCTGCCTGTCTAGTTGTGGAAGCCATGGTGTGGAAATCTGTGACCAAGAACCGAACTTCCTAT
  ATGCGCCACATCTGCATAGTCAACATTGCCTTTTGCCTTCTGATTGCTGACATCTGGTTCATTGTGGCTG
  GTGCTATCCACGACGGTCGCTACCCACTCAACGAAACAGCCTGTGTGGCCGCCACATTCTTCATTCACTT
  CTTCTACCTCAGTGTCTTCTTCTGGATGCTAACTCTAGGCCTCATGCTCTTCTACCGGCTGATTTTCATT
  CTACACGATGCAAGCAAGTCCACTCAGAAAGCCATCGCATTTTCTCTAGGCTATGGCTGTCCCCTCATTA
  TCTCCTCTATCACAGTGGGGGTTACGCAGCCACAGGAAGTCTACATGAGGAAGAACGCGTGTTGGCTCAA
  CTGGGAGGACACCAGAGCACTGCTGGCTTTTGCCATCCCCGCGTTGATTATTGTGGTGGTAAATGTGAGC
  ATCACAGTTGTGGTCATCACCAAGATCCTGAGGCCCTCCATTGGGGACAAGCCAGGCAAGCAAGAGAAGA
  GCAGCCTATTCCAGATCAGCAAGAGTATCGGGGTCCTCACACCACTCTTGGGGCTCACTTGGGGTTTCGG
  TCTTGCCACAGTGATCCAGGGAAGCAATGCTGTGTTCCACATCATATTTACTCTCCTCAATGCCTTCCAG
  GGGCTCTTCATTTTGCTCTTTGGCTGCCTCTGGGATCAGAAGGTGCAGGAAGCTTTGCTGCATAAGTTTT
  CATTGTCAAGGTGGTCTTCTCAACACTCAAAGTCAACATCCATAGGTTCGTCAACACCCGTGTTTTCAAT
  GAGTTCTCCAATATCCCGAAGATTTAATAATCTTTTTGGTAAAACAGGAACATATAACGTTTCCACCCCA
  GAGACAACCAGTTCATCCCTGGAAAATTCCTCTAGTGCTTACTCACTGCTCAACTAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001081178
• Insert Size: 4047 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081178.1, NP_001074647.1
• RefSeq Size: 8535 bp
• RefSeq ORF: 4047 bp
• Locus ID: 224792
• UniProt ID: G5E8Q8
• Cytogenetics: 17 B3
• Gene Summary: Receptor that plays a critical role in lung surfactant homeostasis (PubMed:23590306, PubMed:23922714, PubMed:23684610). May play a role in controlling adipocyte function (PubMed:22971422).[UniProtKB/Swiss-Prot Function]