Disp2 (NM_170593) Mouse Untagged Clone

CAT#: MC224312

Disp2 (untagged) - Mouse dispatched homolog 2 (Drosophila) (Disp2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Disp2
• Synonyms: AI840597; B230210L08Rik; Di; DispB; mKIAA1742
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224312 representing NM_170593
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCCTGAGGCAAGCCCAGAGAGAAGCTGCTCCCTCCACACCTGTCCCCTTGAAGACCCAACCGGTG
  CTCCGGTACCACCACCAACCGTGTCCACGCTCCAGGCTATAGACCCGACGAGTCCGTTAACGGCTGGTCA
  TTTTGCCTTTCCTCGAGCGCCTCAAGACTATCAAGAAGGAAGTTCATTATTAGGATTAGGAGACCAAGCA
  TCTCTATGTGCCCATGTCTCCAACCTCAGCACCTCCATAGACACCTCTCAGCATGATGGGGTCTGGAAGC
  AACCTTCTGTGCAGCGCCATGTGGTCAGTGTCAGGCAGGAACGCACTTTCCGGATGCCGAAGAGCTATTC
  TCATATGATTGCTGATTGGCCAGTGGCTGTGATCGTGGGGTGTCTGGCCTTCATCTTCCTCTGTACCCTA
  GCTGGGCTGTTGGGAAGCCCACCACTTGACTTTTCTGAGCCTTTACTGGGTTTTGAGCCTCGAGACACAG
  AGATAGGCCGAAGGTTAGAAGTTTGGAAAGCCATGCAGGCCCTCACTGGCCCCAAGAATCTGCTTTCTCT
  TTCCCCAGACCCTGAGATGAACAGCTCAAGCCTCCTCAGCACCCTGAGCCCTGCAGCCTGGGGCAGGGCC
  GAGGAAAGTGTGGTCCGGACCAAGAGGATGGTGGGGCCTGTGGAAGTCAAAGAAGAAGAGAACTTCTTTT
  GTGGCCGCCCTGAGAAGAGCCATGCAAAGCTGGTGTTTGTGTCCACCTCTGGGGGAAGCCTGTGGAACCT
  GCAAGCCATCCATTCCATGTGTCGCATAGAACAGGAGCAGATCCGCTCTCACATCAGTTTTGGGGCTCTG
  TGCCAGCGATCAGCAGCCAATGAGTGCTGCCCCAGCTGGTCCTTGGGCAACTATCTGGCCGTGCTGTCTA
  ACCGCTCCTCCTGCCAAGACACTACCCAAGCCGATACAGACCGCACATTGGCCCTGCTACGGTTCTGTGC
  CACCTTCTACCATCGTGGTGTCCTGGTGCCTGCTTGTGTGGGGTCTAGCCAGGACAAACCCCCGTTTTGT
  GCCCAAGTTCCTGCCAAGTGTACCGGAAGCAACGTGGTCTATGAATTCCTGCACTACCTGCTAGACAGAG
  ACTTTCTGAGTCCCCAGACTGCGGATTACCAGGTGCCCTCCCTCAAGTTTGCCCTGCTCTTCCTGCCCAT
  TATAAAGACCTCCTCTCTTCTAGACATCTACCTGGACGGCCTAGGTGACCCAATTAAAGTCTCTGACAAC
  TACACATCTATCAGTGGCATGGACTTGGGCCTCAAGCCCAGACTGCTGAAGTATTACCTAGCCGAAGACA
  CCATGTATCCCTTGATAGCCCTGGTTGTCATCTTCTTTGGCATGTCCCTCTACCTGCGCTCACTCTTCAT
  CACGTTCATGTCACTTCTAGGGGTGCTGGGCTCCCTAATGGTGGCCTACTTTCTTTACCATGTGGCATTC
  CGCATGGCCTACTTCCCCTTTGTCAATCTAGCGGCTCTCCTTCTGCTTAGTGGTGTCTGTGTCAATTACA
  CGCTCATCTTCTTGGATATGTGGCGCCTCAGCAGGGGTCAGGTGCCTTCCGGGGGCATGCCACACCGTGT
  AGGCCGTACCATGCACCACTTTGGCTACCTGCTCCTGGTCTCAGGCCTCACCACCAGCGCAGCCTTCTAC
  GGCAGCTACCTGAGCCGCCTGCCCGCAGTGCGTTGCTTTGCTCTTTTCATGGGCACTGCTGTACTAGTGC
  ACATGGGGCTCACACTGCTCTGGCTTCCCGCCACCGTGGTGCTCCATGAGCGCTACTTGGCTCACGGCTG
  TGTGGCCCAAGCGCACGGCCAGAGGGGTGGCAGCGACCCCCTGAGGCTGTTGCTGGCGTTGCACAGACGG
  ATCCGTATTTTTCGCAAGATTATTTCCATCCTCTCGCGCCTGCTTTTCCAGCGCCTGCTGCCCTGTGGTG
  TCATTAAGTTTCGGTACATCTGGATCTGCTGGTTCGCGGCGTTGGCGGCAGGGGGCGCCTACATCGGCGG
  CGTCAGCCCTCGCCTGCAACTGCCCATTCTATTGCCACTTGGAGGCCAGTTCTTCCGTTCTAGCCATCCC
  TTTGAACGCTTTGACGCAGAGTATCGCCAGCAGTTCCTGTTCGAGGATCTGCCTCCAAACGAGGGCGGCA
  ACTTGCCAGTGGTTCTGGTGTGGGGAATCCTGCCAGTGGACACTAGTGATCCCCTGGACCCTCGCACCAA
  CAGCTCAGTGGTAAGTGATCCTGACTTCTCGCCTAGCAGCCCGGAGGCCCAGGAGTGGCTCCTGGCTCTC
  TGCCATGGAGCCCAGAATCAGAGCTTCTTTGGAGATCAGCCAGAGGGCTGGCCTACGCTGTGTTTAGTGG
  AAGCCTTACAGCAGTGGATGGAGAGCCCTAGCTGTGGCCGCCTGGGTCCTGATCTATGCTGCGGACAATC
  AGAATTCCCCTGGGCCCCCCAGCTTTACCTGCACTGTCTCAAGATGATGGCTCTGGAGCAAAGTCCTGAT
  GGCACACGTGACCTGGGACTCCGCTTCGATACTCATGGCAACCTGGCAGCTCTAGTTCTAAAGTTCCAGA
  CCAACTTACCCTACAGTACAGAGTACGGCCCGGTCCACCACTTCTACACTGAAATCAGCCGCTGGTTGTC
  AACCGAGATGAGCAAGGCACCTCCTGGCCTCAACCAGGGTTGGTTCACCAGCAACTTGGAGCTGTACAGC
  TTGCAGCATAGTCTAAGCACAGAGCCAGCTGTCGTGCTTGGTCTGGCCCTGGCACTAGCCTTTGCCACAC
  TGCTGCTGAGCACCTGGAATGTCCCCCTCAGTCTGTTCTCTGTGGCAGCTGTGGCTGGCACCGTACTGCT
  CACGGTGGGCTTGCTGGTTCTTCTTGAGTGGCAACTCAACACTGCCGAAGCTCTCTTTCTCTCTGCCTCT
  GTGGGCCTCTCTGTAGACTTAACCATTAACTACTGCATCTCCTATCACTTGTGCCCACACCCGGACCGCC
  TGAGCCGTGTGGCCTTCTCTTTACGTCAGATCAGCCGGGCCACGGCGATGACGACTGGAGTGTTGTTTGC
  CTCTGGTGTGATCATGCTGCCTTCCACTATACTGCTCTATCGGAAGCTGGGCATCATCGTCATGATGGTC
  AAGTTCCTTGGCTGTGGCTTTGCCAGCTTCTTCTTTCAGTCCCTGTGCTGTTTCTTCGGGCCAGAAAAGA
  ACTGTGGCCAGATCCTCTGGCCCTGCGCCCATCTGCCGTGGGATGCCGGGACTGAGGATCCTGACGAGAA
  GGGGCGAGCGGGGCCACCAGGATTCTCCGAACACTATGAGTTGCAGCCCCTGGCACGGCGCCGGAGTCCC
  AGCTTCGACACCAGCACAGCCACCAGCAAGCTTTCCCATCGGCCTTCCATACTCTCTGAAGACCTGCAGA
  TACATGATGGCAGCTGCTGCCTCCAGCATGCCCAAGCCCCTGTCTCCCCAAGGGATCTGCTCCTAGACCA
  CCAGACAGTCTTCAGCCAGTGTCCAGCCCTGCAGACCTCCTCTCCATATAAGCAGGCTGGCCCCACCCCC
  CAAACCTGGATCAGGCAAGATTCCCAGGGACAGAAAACTGAGCCCCTGCAGGCACTGCCAGAAGGCCCTG
  CCCACTGCCCTAAGTCCAAAGTGGAAGAGCTCCCCGATGGCCTGTGCTCCTCAGCCAGCACCCTGGAGGG
  ACTCAGCGTCTCAGATGACACCTGTGCCTCTGAGCATAGTGTCCGTGTGCCAGATTCTGTGGGTACCTCC
  CCAGAAGTCATGAATGGCACTGGACACCCCATACTTGAGCGGGGCCAGCTGAATGGGAAGCGCGACACCC
  TCTGGCTAGCACTGAAGGAGACCATCTATGACCCAAACATGCCCAATTCTCACCACAGCAGCTTATCCTG
  GAAGGGCCGTGGAGGGCCAGGCGATATCAGCCCTGTGATGCTTCCCAACAGTCAGCCAGATCTCCCAGAT
  GTTTGGCTCCGTAGGCCCAGCACCTACACCTCTGGCTACAGCAGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_170593
• Insert Size: 4038 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_170593.3, NP_733481.1
• RefSeq Size: 6629 bp
• RefSeq ORF: 4038 bp
• Locus ID: 214240
• UniProt ID: Q8CIP5
• Cytogenetics: 2 E5
• Gene Summary: The pattern of cellular proliferation and differentiation that leads to normal development of embryonic structures often depends upon the localized production of secreted protein signals. Cells surrounding the source of a particular signal respond in a graded manner according to the effective concentration of the signal, and this response produces the pattern of cell types constituting the mature structure. A segment-polarity gene known as dispatched has been identified in Drosophila and its protein product is required for normal Hedgehog (Hh) signaling. [provided by RefSeq, Sep 2015]