Ptprj (NM_008982) Mouse Untagged Clone

CAT#: MC224301

Ptprj (untagged) - Mouse protein tyrosine phosphatase, receptor type, J (Ptprj), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ptprj
• Synonyms: AI450271; BET; Byp; CD148; DEP-1; Ptpb2; PTPbeta2; RPTPJ; Scc-1; Scc1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224301 representing NM_008982
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGCGCGGAGGAGGCAGAGGGAGCAGCCGGGGCCGCGGGAGCCGGGAGCTGGGAGCCACGCGCGGAG
  GGTGGGCGCCGCTCGCTCCGCCCCGCGAAGCCCCTGCGAGTCTAAGGCCGCGGCCGCTCCGCGCCAGGCG
  CGCTAGGCTCCGCCGTGTGGCCGCCGCCGCCGCCGCTGCCATGTCCCCGGGGAAGCCCGGGGCGGGCGGA
  GCGGGGACTAGGCGGACCGGCTGGCGGAGAAGGAGGCGGAGGCGTCGGCTGGAGACGGAGACGAGGGCGC
  CCGGCTTCGGGCACACGGCGGGGCGCGTCCCGGGCACGTTCCAGGGCGCGCAGGGCATGAAGCCCGCGGC
  GCGGGAGACGCGGACACCCCCGCGCTCGCCCGGGCTCCGCTGGGCGCTGCTGCCGCTGCTGCTGTTGCTA
  CGCCAGGGCCAGGTCCTGTGCGCAGGTGCGGCACCGAATCCTATATTTGACATTGAAGCTGTCGTCAGCC
  CAACTAGTGTGTTATTAACATGGAAGCACAATGACTCAGGCGCTTCAGAATGTAGAATAGAGAATAAGAT
  GGAGAGCAATCTGACGTTTCCTGTTAAAAACCAGACATCATGTAACATTACAGGCTTAAGCCCAGGCACT
  TCGTATACATTCTCCATCATCTCTGTAACAACCAATGAGACCTTGAACAAAACTATCACAACAGAGCCCT
  GGCCAGTGTCTGATCTCCATGTCACCTCTGTGGGTGTGACACAGGCTCGTCTCACCTGGAGCAATGCAAA
  TGGCACTGCCTCCTACCGGATGCTGATTGAAGAGTTGACCACACATTCCTCAGTCAATATTTCAGGTCTG
  AAGCCGGGGACCAATAATAGCTTCGCTTTCCCAGAATCAAATGAGACACAGGCTGACTTTGCAGTTGCAG
  AGGAGGTCCCGGATGCCAATGGTACCAAGAGAATCCCAGTGACCAACCTATCCCAACTACACAAGAATTC
  TCTTGTCTCTGTGGACCCACCCTCTGGCCAGGATCCCTCCCTCACAGAGATCTTGCTTACTGACCTAAAG
  CCTGATACTCAGTACAATGCCACCATCTATTCTCAAGCAGCAAATGGCACTGAAGGACAGCCCAGGAACA
  AAGTGTTTAAAACAAATTCCACCCAGGTTTCTGACGTCCGAGCTATGAACATCAGTGCCTCAAGCATGAC
  CCTGACCTGGAAAAGCAATTACGATGGGTCCCGTACTTCAATTGTCTACAAAATACACGTGGCTGGGGGG
  ACCCACTCCGTCAACCAAACTGTCAATAAGACTGAGGCCATCATCCTCGGACTCAGCTCCAGCACCTTGT
  ACAACATCACAGTTCATCCTTTCCTGGGTCAGACGGAGGGCACACCAGGCTTCCTCCAAGTGTACACTTC
  CCCCGATCAGGTCTCTGACTTCCGAGTGACAAATGTCAGCACAAGGGCAATTGGTTTGGCTTGGAGGAGC
  AATGACTCCAAGTCCTTCGAGATTTTCATCAAGCAGGACGGAGGTGAGAAGCATCGAAATGCTTCGACGG
  GAAACCAGAGCTATATGGTTGAAGATTTAAAGCCTGGAACCAGTTACCATTTTGAGATAATTCCACGAGG
  ACCAGACGGGACAGAAGGGCTGTCCAGTACAGTGAATGGGAGCACTGACCCCAGTGCTGTGACTGACATC
  CGGGTGGTCAACATTAGCACCACTGAAATGCAGTTGGAGTGGCAGAATACGGACGATGCCTCTGGATACA
  CTTACCATTTAGTTCTAGAGTCTAAAAGTGGCTCCATCATCAGGACCAACAGTTCTCAGAAGTGGATCAC
  AGTAGGGAGCCTCACCCCAGGCACCTTATACAATGTCACAATCTTTCCAGAAGTGGACCAGATCCAGGGA
  ATCTCCAACTCCATTACCCAGTACACACGGCCCAGCAGTGTGTCCCACATTGAAGTAAACACCACCACCA
  CCACGGCAGCCATCCGATGGAAGAACGAGGACGCAGCCTCTGCTTCCTATGCCTACTCCGTCCTTATCTT
  GAAGACTGGAGATGGCAGCAATGTAACCAGCAACTTCACAAAAGACCCTTCTATTCTAATCCCTGAGTTA
  ATCCCTGGTGTCTCTTACACAGTGAAGATCCTTACACAAGTTGGGGATGGTACAACATCACTGGTACCTG
  GTTGGAATCTGTTCTGTACGGAACCTGAACCAGTGACCTCCTTCCACTGTGAAGTGGTCCCTAAGGAGCC
  AGCATTGGTTCTCAAGTGGGCCTGCCCCTTTGGCATGTACACAGGCTTCGAGCTGGGGGTCAGGAGTGAT
  TCCTGGGACAATATGACACGCCTAGAGAACTGCACTTCGGATGATGACACAGAGTGCAGGACGGAAGTCG
  CCTATTTGAATTTTTCTACCTCGTACAACATCAGCATCGCCACCTTGTCATGTGGGAAGATGGCGCTTCC
  CGCCCAGAACATCTGCACCACTGGCATCACAGACCCACCTACTCCGGATGGATCCCCTAATATTACATCG
  GTCAGTCACAATTCAGTAAAGGTTAAGTTCAGCGGGTTTGAAGCCAGCCACGGACCTATCAAAGCCTATG
  CTGTCATCCTCACCACCGGGGAAGCTGCCCAACCTTCTGCAGATGTTTTGAAGTACACGTATGAGGATTT
  CAAAAGGGGAGCCTCGGATACTTATGTCACATACCTCATAAGAATAGAAGAGAAGGGACAGTCTCAGGGC
  TTGTCTGAAGTCTTGAACTATGAAATTGATGTGGGGAACCAATCCACTACCCTCGGCTACTACAACGGGA
  GGCTGGAGCCTCTGGGCTCCTACCGGGCTTGTGTTGCTGGCTTTACCAATATTACCTACAACCTTCAGAA
  TGACGGCCTCATCAATGGGGATGAGAGCTATGTGTCTTTCAGTCCATATTCAGAGGCCGTGTTCTTGCCC
  CAGGACCCAGGTGTCATCTGCGGAGCAGTGTTTGGATGTATCTTTGGTGCCCTGGCCATCACAGCTGTGG
  GAGGCTTCATCTTCTGGAGAAAGAAAAGGACAGATGCCAAGAATAATGAAGTGTCCTTTTCTCAAATTAA
  ACCTAAAAAATCCAAGTTAATCCGAGTGGAGAATTTTGAGGCCTACTTTAAGAAGCAGCAAGCTGACTCT
  AACTGTGGGTTTGCAGAGGAATATGAGGACCTGAAGCTGATTGGGATAAGTTTACCTAAATACACAGCTG
  AGATAGCCGAGAACAGAGGGAAGAACCGCTACAACAATGTTCTGCCCTATGATATTTCTCGAGTCAAACT
  TTCAGTCCAGACCCATTCGACAGATGACTACATCAATGCCAACTATATGCCTGGCTACCATTCCAAGAAA
  GATTTCATTGCCACACAAGGACCTTTACCCAACACTTTGAAAGATTTCTGGCGTATGGTTTGGGAGAAAA
  ACGTATATGCCATTGTTATGTTGACCAAATGCGTGGAGCAGGGAAGGACCAAATGTGAGGAGTACTGGCC
  TTCCAAGCAGGCTCAGGACTACGGGGACATAACTGTGGCGATGACATCAGAAGTCGTTCTTCCAGAATGG
  ACCATCAGAGATTTTGTGGTGAAAAATATGCAGAATAGCGAGAGCCATCCTCTGCGGCAGTTCCATTTCA
  CCTCCTGGCCTGACCACGGTGTTCCTGACACCACTGACCTGCTCATCAACTTTCGGTACCTGGTCCGGGA
  TTACATGAAGCAGATACCCCCCGAGTCACCAATTCTGGTGCATTGCAGTGCTGGGGTTGGAAGGACGGGC
  ACTTTCATCGCCATCGATCGCCTGATCTATCAGATAGAGAATGAGAACACCGTGGACGTGTATGGGATTG
  TCTATGACCTTCGGATGCACAGGCCTCTGATGGTGCAGACAGAGGACCAGTATGTTTTCCTCAATCAGTG
  TGTTTTGGATATTATCAGAGCCCAGAAAGACTCAAAAGTTGATCTCATCTATCAGAACACAACGGCAATG
  ACAATCTATGAAAACCTCGAGCCAGTGAGCATGTTTGGAAAGACTAATGGTTACATCGCCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008982
• Insert Size: 4053 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008982.5, NP_033008.3
• RefSeq Size: 7634 bp
• RefSeq ORF: 4053 bp
• Locus ID: 19271
• UniProt ID: Q64455
• Cytogenetics: 2 50.19 cM
• Gene Summary: Tyrosine phosphatase which dephosphorylates or contributes to the dephosphorylation of CTNND1, FLT3, PDGFRB, MET, RET, KDR, LYN, SRC, MAPK1, MAPK3, EGFR, TJP1, OCLN, PIK3R1 and PIK3R2. Plays a role in cell adhesion, migration, proliferation and differentiation. Involved in vascular development. May be involved in the mechanism of contact inhibition of cell growth. Regulator of macrophage adhesion and spreading. Positively affects cell-matrix adhesion. Positive regulator of platelet activation and thrombosis. Negative regulator of cell proliferation. Negative regulator of PDGF-stimulated cell migration; through dephosphorylation of PDGFR. Positive regulator of endothelial cell survival, as well as of VEGF-induced SRC and AKT activation; through KDR dephosphorylation. Negative regulator of EGFR signaling pathway; through EGFR dephosphorylation. Enhances the barrier function of epithelial junctions during reassembly. Negatively regulates T-cell receptor (TCR) signaling. Upon T-cell TCR activation, it is up-regulated and excluded from the immunological synapses, while upon T-cell-antigen presenting cells (APC) disengagement, it is no longer excluded and can dephosphorylate PLCG1 and LAT to down-regulate prolongation of signaling.[UniProtKB/Swiss-Prot Function]